Papers by Nikolai Lazarov
Dokladi na Bʺlgarskata akademiâ na naukite, Apr 26, 2024
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Advances in Anatomy Embryology and Cell Biology, Dec 31, 2022
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Advances in Anatomy Embryology and Cell Biology, Dec 31, 2022
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Advances in Anatomy Embryology and Cell Biology, Dec 31, 2022
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Advances in Anatomy Embryology and Cell Biology, Dec 31, 2022
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Advances in Anatomy Embryology and Cell Biology, Dec 31, 2022
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Advances in Anatomy Embryology and Cell Biology, Dec 31, 2022
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Advances in Anatomy Embryology and Cell Biology, Dec 31, 2022
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Advances in Anatomy Embryology and Cell Biology, Dec 31, 2022
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Advances in Anatomy Embryology and Cell Biology, Dec 31, 2022
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Biomedical Reviews/Bio-Medical Reviews, Dec 31, 2023
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trakia journal of sciences, 2019
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Methods in molecular biology (Clifton, N.J.), 2017
Enzyme histochemical methods are valuable for the studies on the enzyme involvement in different ... more Enzyme histochemical methods are valuable for the studies on the enzyme involvement in different pathological processes. Here we describe two protocols for chromogenic and fluorogenic histochemical demonstration of tripeptidyl aminopeptidase I (TPPI), a protease that is crucial for neuronal functions. The procedures are based on newly synthesized substrates for TPPI-glycyl-L-prolyl-L-metionyl-5-chloro-1-anthraquinonylhydrazide (GPM-CAH) and glycyl-L-prolyl-L-metionyl-4-hydrazido-N-hexyl-1,8-naphthalimide (GPM-HHNI). Using such protocols, precise enzyme localization can be obtained in tissue sections of mammalian organs.
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Morphofunctional and Neurochemical Aspects of the Mammalian Carotid Body
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Morphofunctional and Neurochemical Aspects of the Mammalian Carotid Body
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Scripta scientifica medica, Oct 10, 2017
Inflammatory signal molecules are suggested to be involved in the mechanism underlying comorbid d... more Inflammatory signal molecules are suggested to be involved in the mechanism underlying comorbid depression in epilepsy. The aim of this study was to examine the effect of the novel antidepressant agomelatine (Ago), a potent melatonin MT1 and MT2 receptor agonist and serotonin 5HT2C receptor antagonist, on glial activity during kainate (KA)-induced epileptogenesis.Brain sections were immunostained with the microglial marker Iba-1 and two astrocyte-specific markers, GFAP and S100 beta, and the glial cell morphology in brain structures vulnerable to KA-induced neurotoxicity was described in the KA-treated groups and their matched controls, respectively.Both control and Ago-treated groups were characterized with ramified microglial cells with fine processes. After administration of KA, activated Iba-1-immunoreactive microglial cells in the dorsal and ventral hippocampus, dentate gyrus, as well as in the basolateral amygdala (BLA) and piriform cortex were increased in number and displayed enlarged round-shaped bodies and arborization. Chronic Ago treatment during epileptogenesis significantly diminished their number compared to that in the matched vehicle-treated epileptic group, specifically in the CA1a, CA1b, CA1c and CA3c subfields of dorsal hippocampus, BLA and piriform cortex. Conversely, the KA-induced reactive astrogliosis was more restricted and both GFAP- and S100 beta-immunostained cells were observed mainly in the CA1 and CA3c fields of dorsal hippocampus and piriform cortex. The Ago treatment did not significantly reduce the number of immunopositive astrocytes in the CA2, CA3a, CA3b subfields of the dor sal and CA1, CA2, CA3 subfields of the ventral hippocampus, the dentate gyrus and the BLA. In addition, only a few immunostained ramified astrocytes were scattered in the ventral hippocampus of the KA-veh group, while the Ago-treated group showed a considerable increase of immunoreactive cells in this area compared to controls.The present results show that chronic Ago treatment during epileptogenesis prevents the KA-induced microglial activation and suppresses the neuronal cell death in the vulnerable limbic regions. It can provide effective therapy for brain disorders involving neuronal damage, neuroinflammation and oxidative stress such as epilepsy
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Scripta scientifica medica, Oct 10, 2017
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Papers by Nikolai Lazarov