Papers by Sameh S. Zaghlool
Biomedicine & Pharmacotherapy, 2022
Pulmonary fibrosis (PF) is a chronic progressive disease that portends a very poor prognosis. It ... more Pulmonary fibrosis (PF) is a chronic progressive disease that portends a very poor prognosis. It has been suggested that STAT3 is a potential target in PF. This study highlights the importance of cubosomes as a drug delivery system in enhancing the bioavailability of nifuroxazide (NXZD), a poorly soluble STAT3 inhibitor. NXZD-loaded cubosomes (NXZD-LC) were in vitro and in vivo evaluated. In vitro, cubosomes presented a poly-angular nanosized particles with a mean size and zeta potential of 223.73 ± 4.73 nm and - 20.93 ± 2.38 mV, respectively. The entrapment efficiency of nifuroxazide was 90.56 ± 4.25%. The in vivo pharmacokinetic study and the lung tissue accumulation of NXZD were performed by liquid chromatography-tandem mass spectrometry after oral administration to rats. The nanoparticles exhibited a two-fold increase and 1.33 times of bioavailability and lung tissue concentration of NXZD compared to NXZD dispersion, respectively. In view of this, NXZD-LC effectively attenuated PF by targeting STAT3 and NF-κB signals. As a result, NXZD-LC showed a potential anti-inflammatory effect as revealed by the significant decrease in MCP-1, ICAM-1, IL-6, and TNF-α and suppressed fibrogenic mediators as indicated by the significant reduction in TGF-β, TIMP-1, and PDGF-BB in lung tissues. Besides, NXZD-LC improved antioxidant defense mechanisms and decreased LDH and BALF total protein. These effects contributed to decreased collagen deposition. To conclude, cubosomes represent an advantageous pharmaceutical delivery system for enhancing pulmonary delivery of poorly soluble drugs. Additionally, repurposing NXZD as an antifibrotic agent is a promising challenge and new therapeutic approach for unmet therapeutic needs.
Limited pharmacological studies have been conducted on plant species used against poultry helmint... more Limited pharmacological studies have been conducted on plant species used against poultry helminths. The objective of this study was to provide a basis for plant based anthelmintics as possible alternatives against poultry anthelmintic resistance. The study justified the need for alternative anthelmintics. The study places emphasis on the increasing anthelmintic resistance, mechanism of resistance, and preparational protocols for plant anthelmintics and their associated mechanism of action. Pharmaceutical studies on plants as alternative therapies for the control of helminth parasites have not been fully explored especially in several developing countries. Plants from a broad range of species produce a wide variety of compounds that are potential anthelmintics candidates. Important phenolic acids have been found in Brassica rapa L. and Terminalia avicenniodes Guill. and Perri that affect the cell signaling pathways and gene expression. Benzo (c) phenanthridine and isoquinoline alkal...
Peptic ulcer is one the most serious diseases over wide the world. Most classic treatment lines p... more Peptic ulcer is one the most serious diseases over wide the world. Most classic treatment lines produce adverse drug reactions. Therefore, this study aimed to investigate the protective effects of two natural extracts, namely ginger and marshmallow extracts, on peptic ulcer induced experimentally in rats compared to famotidine as a reference anti-ulcer agent. In the present study, two different models of animal experiments have been involved. The first was rat pyloric ligation-induced gastric ulceration model. The second model of experiments was indomethacin-induced gastric ulceration in rats. Results demonstrate that administration of either ginger or marshmallow extracts could protect against pyloric ligation-induced peptic ulcer and indomethacin-induced peptic ulcer in rats presumably via their antioxidant properties, being promising for further clinical trials.
UK Journal of Pharmaceutical Biosciences, 2015
Gastric ulcer is one of the most gastro-intestinal disorders in world. There are many drugs used ... more Gastric ulcer is one of the most gastro-intestinal disorders in world. There are many drugs used for the treatment of gastric ulcer, but most of these associates with several adverse effects. In the present study we investigate the protective effects of extracts of Zingiber officinale and Althaea officinalis on pyloric ligation-induced gastric ulcer in rats. Animals were divided into 5 groups; a normal control group, an ulcer control group, a standard treatment group receiving famotidine (20 mg/kg), and two treatment groups receiving Z. officinale extract (100 mg/kg) and A.officinalis extract (100 mg/kg). Treatments were given orally for 14 days. On the 15th day, animals were subjected to pyloric ligation except normal control group. Four hours later, rat stomachs were excised and gastric juice and blood samples were collected. Pyloric ligation significant increases ulcer number, ulcer index, gastric volume, titratble acidity, acid output, mucin content and peptic activity, accompan...
Journal of Bioequivalence & Bioavailability, 2015
Gastric ulcer is one of the most serious diseases over wide the world. There are many drugs used ... more Gastric ulcer is one of the most serious diseases over wide the world. There are many drugs used for the treatment of gastric ulcer, but most of these produce several adverse reactions. This study aims to investigate the protective effects of extracts of Ginger and Marshmallow on pyloric ligation-induced gastric ulcer in rats. Animals were divided into 5 Groups; a normal control group, an ulcer control group, a standard treatment group receiving famotidine (20 mg/kg), and two treatment groups receiving Ginger (100 mg/kg) and Marshmallow (100 mg/kg). Treatments were given orally for 14 days. On the 15th day, animals were subjected to pyloric ligation except for the normal control group. Four hours later, rat stomachs were excised and gastric juice and blood samples were collected. Pyloric ligation caused significant elevations in ulcer number, ulcer index, gastric volume, titratble acidity, acid output, mucin content and peptic activity, accompanied by significant decreases in blood ...
Journal of Natural Science, Biology and Medicine, 2015
Pharmaceuticals
Background: Alzheimer’s disease (AD) is a neurodegenerative disorder that is associated with abno... more Background: Alzheimer’s disease (AD) is a neurodegenerative disorder that is associated with abnormal cognition. AD is aided in its initiation and progression by hereditary and environmental factors. Aluminum (Al) is a neurotoxic agent that causes oxidative stress, which is linked to AD progression. Additionally, Nrf2/HO-1, APOE4/LRP1, Wnt3/β-catenin, and TLR4/NLRP3 are the main signaling pathways involved in AD pathogenesis. Several phytochemicals are promising options in delaying AD evolution. Objectives: This study aimed at studying the neuroprotective effects of some phytochemicals as morin (MOR), thymol (TML), and thymoquinone (TMQ) on physical and mental activities (PhM) in Al chloride (AlCl3)-induced AD rat model. Another objective was to determine the specificity of phytochemicals to AD signaling pathways using molecular docking. Methods: Eighty male Dawley rats were divided into eight groups. Each group received: saline (control group), AlCl3, (ALAD), PhM, either alone or w...
Antioxidants
Recently, an alternative disease treatment approach is the research of medicaments from tradition... more Recently, an alternative disease treatment approach is the research of medicaments from traditional medicine. Plants with anti-oxidant capabilities are used as herbal treatments for ulcer diseases. Medicinal/herbal extracts containing phytoconstituents have significant anti-ulcer activities in in vivo experiments on animal models, compared to reference drugs. The current study aims to inspect gastro-protective as well as in vitro and in vivo anti-oxidant potential of Althaea officinalis and Solanum nigrum extracts on pyloric-ligation/indomethacin-induced gastric-ulceration in rats. Rats were divided into six groups: normal control, gastric ulcer control, two standard pretreatment groups receiving omeprazole and misoprostol, and two test pretreatment groups receiving Althaea officinalis and Solanum nigrum. Pretreatments were administrated orally for 14 days. On the 15th day, animals, excluding the normal control group, were exposed to pyloric-ligation followed by indomethacin injecti...
1. Ginger and Marshmallow Extracts Can Protect against Pyloric Ligation-Induced Peptic Ulcer in R... more 1. Ginger and Marshmallow Extracts Can Protect against Pyloric Ligation-Induced Peptic Ulcer in Rats 2. Ginger and Marshmallow Extracts Can Protect against Indomethacin-Induced Peptic Ulcer in Rats
Gastric ulcer is one of the most gastro-intestinal disorders in world. There are many drugs used ... more Gastric ulcer is one of the most gastro-intestinal disorders in world. There are many drugs used for the treatment of gastric ulcer, but most of these associates with several adverse effects. In the present study we investigate the protective effects of extracts of Zingiber officinale and Althaea officinalis on pyloric ligation-induced gastric ulcer in rats. Animals were divided into 5 groups; a normal control group, an ulcer control group, a standard treatment group receiving famotidine (20 mg/kg), and two treatment groups receiving Z. officinale extract (100 mg/kg) and A.officinalis extract (100 mg/kg). Treatments were given orally for 14 days. On the 15th day, animals were subjected to pyloric ligation except normal control group. Four hours later, rat stomachs were excised and gastric juice and blood samples were collected. Pyloric ligation significant increases ulcer number, ulcer index, gastric volume, titratble acidity, acid output, mucin content and peptic activity, accompanied by significant decreases in blood superoxide dismutase activity (SOD) activity and gastric mucosal nitric oxide (NO) and glutathione (GSH) contents. In addition, elevations in gastric mucosal lipid peroxide and histamine contents were observed. Pretreatment with famotidine, Z. officinale or A.officinalis extracts significantly corrected all blood and tissue parameters by varying degrees.Famotidine, Z. officinale and A.officinalis extracts may protect against pyloric ligation-induced peptic ulcer in rats, being promising for further clinical trials.
Background: Gastric ulcer is one of the most serious diseases over wide the world. There are many... more Background: Gastric ulcer is one of the most serious diseases over wide the world. There are many drugs used for the treatment of gastric ulcer, but most of these produce several adverse reactions. This study aims to investigate the protective effects of extracts of ginger and marshmallow on pyloric ligation-induced gastric ulcer in rats.
Methods: Animals were divided into 5 Groups; a normal control group, an ulcer control group, a standard treatment group receiving famotidine (20 mg/kg), and two treatment groups receiving ginger (100 mg/kg) and marshmallow (100 mg/kg). Treatments were given orally for 14 days. On the 15th day, animals were subjected to pyloric ligation except for the normal control group. Four hours later, rat stomachs were excised and gastric juice and blood samples were collected.
Results: Pyloric ligation caused significant elevations in ulcer number, ulcer index, gastric volume, titratble acidity, acid output, mucin content and peptic activity, accompanied by significant decreases in blood superoxide dismutase (SOD) activity and gastric mucosal nitric oxide (NO) and glutathione (GSH) contents. In addition, elevations in gastric mucosal lipid peroxide and histamine contents were observed. Pretreatment with famotidine, ginger or marshmallow significantly corrected all blood and tissue parameters by varying degrees.
Conclusions: Famotidine, ginger and marshmallow may protect against pyloric ligation-induced peptic ulcer in rats, being promising for further clinical trials.
Background: Gastric ulcer is one of the most serious diseases. Most classic treatment lines produ... more Background: Gastric ulcer is one of the most serious diseases. Most classic treatment lines produce adverse drug reactions.
Therefore, this study aimed to investigate the protective effects of two natural extracts, namely ginger and marshmallow extracts,
on indomethacin-induced gastric ulcer in rats. Materials and Methods: Animals were divided into five groups; a normal control
group, an ulcer control group, and three treatment groups receiving famotidine (20 mg/kg), ginger (100 mg/kg), and marshmallow
(100 mg/kg). Treatments were given orally on a daily basis for 14 days prior to a single intra-peritoneal administration of indomethacin
(20 mg/kg). Results: Indomethacin administration resulted in significant ulcerogenic effect evidenced by significant elevations
in ulcer number, ulcer index, and blood superoxide dismutase activity accompanied by significant decreases in gastric mucosal
nitric oxide and glutathione levels. In addition, elevations in gastric mucosal lipid peroxides and histamine content were observed.
Alternatively, pretreatment with famotidine, ginger or marshmallow significantly corrected macroscopic and biochemical findings,
supported microscopically by results of histopathological study. Conclusion: These results demonstrate that administration
of either ginger or marshmallow extract could protect against indomethacin-induced peptic ulcer in rats presumably via their
antioxidant properties and inhibition of histamine release.
Gastric ulcer is one of the most serious diseases over wide the world. There are many drugs used ... more Gastric ulcer is one of the most serious diseases over wide the world. There are many drugs used for the treatment of gastric ulcer, but most of these produce several adverse reactions. This study aims to investigate the protective effects of extracts of Ginger and Marshmallow on pyloric ligation-induced gastric ulcer in rats. Animals were divided into 5 Groups; a normal control group, an ulcer control group, a standard treatment group receiving famotidine (20 mg/kg), and two treatment groups receiving Ginger (100 mg/kg) and Marshmallow (100 mg/kg). Treatments were given orally for 14 days. On the 15th day, animals were subjected to pyloric ligation except for the normal control group. Four hours later, rat stomachs were excised and gastric juice and blood samples were collected. Pyloric ligation caused significant elevations in ulcer number, ulcer index, gastric volume, titratble acidity, acid output, mucin content and peptic activity, accompanied by significant decreases in blood superoxide dismutase activity (SOD) activity and gastric mucosal nitric oxide (NO) and glutathione (GSH) contents. In addition, elevations in gastric mucosal lipid peroxide and histamine contents were observed. Pretreatment with famotidine, ginger or marshmallow significantly corrected all blood and tissue parameters by varying degrees. Famotidine, ginger and marshmallow may protect against pyloric ligation-induced peptic ulcer in rats, being promising for further clinical trials.
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Papers by Sameh S. Zaghlool
Methods: Animals were divided into 5 Groups; a normal control group, an ulcer control group, a standard treatment group receiving famotidine (20 mg/kg), and two treatment groups receiving ginger (100 mg/kg) and marshmallow (100 mg/kg). Treatments were given orally for 14 days. On the 15th day, animals were subjected to pyloric ligation except for the normal control group. Four hours later, rat stomachs were excised and gastric juice and blood samples were collected.
Results: Pyloric ligation caused significant elevations in ulcer number, ulcer index, gastric volume, titratble acidity, acid output, mucin content and peptic activity, accompanied by significant decreases in blood superoxide dismutase (SOD) activity and gastric mucosal nitric oxide (NO) and glutathione (GSH) contents. In addition, elevations in gastric mucosal lipid peroxide and histamine contents were observed. Pretreatment with famotidine, ginger or marshmallow significantly corrected all blood and tissue parameters by varying degrees.
Conclusions: Famotidine, ginger and marshmallow may protect against pyloric ligation-induced peptic ulcer in rats, being promising for further clinical trials.
Therefore, this study aimed to investigate the protective effects of two natural extracts, namely ginger and marshmallow extracts,
on indomethacin-induced gastric ulcer in rats. Materials and Methods: Animals were divided into five groups; a normal control
group, an ulcer control group, and three treatment groups receiving famotidine (20 mg/kg), ginger (100 mg/kg), and marshmallow
(100 mg/kg). Treatments were given orally on a daily basis for 14 days prior to a single intra-peritoneal administration of indomethacin
(20 mg/kg). Results: Indomethacin administration resulted in significant ulcerogenic effect evidenced by significant elevations
in ulcer number, ulcer index, and blood superoxide dismutase activity accompanied by significant decreases in gastric mucosal
nitric oxide and glutathione levels. In addition, elevations in gastric mucosal lipid peroxides and histamine content were observed.
Alternatively, pretreatment with famotidine, ginger or marshmallow significantly corrected macroscopic and biochemical findings,
supported microscopically by results of histopathological study. Conclusion: These results demonstrate that administration
of either ginger or marshmallow extract could protect against indomethacin-induced peptic ulcer in rats presumably via their
antioxidant properties and inhibition of histamine release.
Methods: Animals were divided into 5 Groups; a normal control group, an ulcer control group, a standard treatment group receiving famotidine (20 mg/kg), and two treatment groups receiving ginger (100 mg/kg) and marshmallow (100 mg/kg). Treatments were given orally for 14 days. On the 15th day, animals were subjected to pyloric ligation except for the normal control group. Four hours later, rat stomachs were excised and gastric juice and blood samples were collected.
Results: Pyloric ligation caused significant elevations in ulcer number, ulcer index, gastric volume, titratble acidity, acid output, mucin content and peptic activity, accompanied by significant decreases in blood superoxide dismutase (SOD) activity and gastric mucosal nitric oxide (NO) and glutathione (GSH) contents. In addition, elevations in gastric mucosal lipid peroxide and histamine contents were observed. Pretreatment with famotidine, ginger or marshmallow significantly corrected all blood and tissue parameters by varying degrees.
Conclusions: Famotidine, ginger and marshmallow may protect against pyloric ligation-induced peptic ulcer in rats, being promising for further clinical trials.
Therefore, this study aimed to investigate the protective effects of two natural extracts, namely ginger and marshmallow extracts,
on indomethacin-induced gastric ulcer in rats. Materials and Methods: Animals were divided into five groups; a normal control
group, an ulcer control group, and three treatment groups receiving famotidine (20 mg/kg), ginger (100 mg/kg), and marshmallow
(100 mg/kg). Treatments were given orally on a daily basis for 14 days prior to a single intra-peritoneal administration of indomethacin
(20 mg/kg). Results: Indomethacin administration resulted in significant ulcerogenic effect evidenced by significant elevations
in ulcer number, ulcer index, and blood superoxide dismutase activity accompanied by significant decreases in gastric mucosal
nitric oxide and glutathione levels. In addition, elevations in gastric mucosal lipid peroxides and histamine content were observed.
Alternatively, pretreatment with famotidine, ginger or marshmallow significantly corrected macroscopic and biochemical findings,
supported microscopically by results of histopathological study. Conclusion: These results demonstrate that administration
of either ginger or marshmallow extract could protect against indomethacin-induced peptic ulcer in rats presumably via their
antioxidant properties and inhibition of histamine release.