The present study was conceptualized to study the effect of explants and plant growth regulators ... more The present study was conceptualized to study the effect of explants and plant growth regulators on organogenesis to establish an efficient regeneration protocol for Coleus barbatus, an endangered ethnomedicinal Plant. In course of this, sterilized explants were cultured onto MS medium augmented with different concentrations and combinations of auxin (IAA) and cytokinin (BAP). The best callogenic response was observed on media M4 followed by M9 and M5 in terms of Days of callogenesis, degree of response, colour and texture, considering all the three explants. Regenerated shoots from embryogenic calli were further multiplied on fresh media. Highest per cent shoot regeneration response was obtained on medium M12 (84.7%) and M7 (84.7%) followed by media M6 (81.4%), whereas in terms of number of shoots per culture, shoot apex (0.0-3.7) and internodal stem (0.0-3.7) exhibited as better explant then young leaf (0.0-1.7). The long and healthy shoots were harvested and subcultured on rootin...
Kali haldi (Curcuma caesia) is a medicinal plant. The rhizome of the plant traditionally known fo... more Kali haldi (Curcuma caesia) is a medicinal plant. The rhizome of the plant traditionally known for treating various diseases such as leukoderma, asthma, tumours, piles, leprosy, epilepsy, cancer by the tribal peoples. The medicinal properties of Kali haldi might be various phytochemical constituents present inside the plant. In present investigation an attempt was taken to induce callus for its in vitro propagation and phytochemical extraction. For inducing callus, MS media supplemented with various combinations and concentrations of 2, 4-D, BAP, IAA and NAA was used. The most suitable media composition on which 90.28% callus generated was MS medium added with CaCl2 and supplemented with 2,4D (1.0/ mglˉ) and NAA (0.5/mglˉ) phytohormones.
The present study was conceptualized to study the effect of explants and plant growth regulators ... more The present study was conceptualized to study the effect of explants and plant growth regulators on organogenesis to establish an efficient regeneration protocol for Coleus barbatus, an endangered ethnomedicinal Plant. In course of this, sterilized explants were cultured onto MS medium augmented with different concentrations and combinations of auxin (IAA) and cytokinin (BAP). The best callogenic response was observed on media M4 followed by M9 and M5 in terms of Days of callogenesis, degree of response, colour and texture, considering all the three explants. Regenerated shoots from embryogenic calli were further multiplied on fresh media. Highest per cent shoot regeneration response was obtained on medium M12 (84.7%) and M7 (84.7%) followed by media M6 (81.4%), whereas in terms of number of shoots per culture, shoot apex (0.0-3.7) and internodal stem (0.0-3.7) exhibited as better explant then young leaf (0.0-1.7). The long and healthy shoots were harvested and subcultured on rootin...
Kali haldi (Curcuma caesia) is a medicinal plant. The rhizome of the plant traditionally known fo... more Kali haldi (Curcuma caesia) is a medicinal plant. The rhizome of the plant traditionally known for treating various diseases such as leukoderma, asthma, tumours, piles, leprosy, epilepsy, cancer by the tribal peoples. The medicinal properties of Kali haldi might be various phytochemical constituents present inside the plant. In present investigation an attempt was taken to induce callus for its in vitro propagation and phytochemical extraction. For inducing callus, MS media supplemented with various combinations and concentrations of 2, 4-D, BAP, IAA and NAA was used. The most suitable media composition on which 90.28% callus generated was MS medium added with CaCl2 and supplemented with 2,4D (1.0/ mglˉ) and NAA (0.5/mglˉ) phytohormones.
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