Significance of a short-time survival test for the prediction of quality parameters of sperm obta... more Significance of a short-time survival test for the prediction of quality parameters of sperm obtained by gradient centrifugation was evaluated from an aspect of their functional resistance. Frozen semen samples from 27 bulls before and after separation were assessed by a 120-min survival test. The evaluation of morphological examination of ejaculates was carried out using the SASMO computer program. Statistically significant interrelationship between sperm quality parameters in the initial insemination doses, the amounts of obtained sperm after separation and their functional and vital resistance in the survival test was documented.
Objective This study was focused on the influence of the skimmed milk-based extender (SM), the IN... more Objective This study was focused on the influence of the skimmed milk-based extender (SM), the INRA 96 extender and BotuSemen Gold extender on parameters of stallions' ejaculate during storage. Methods In this study, 14 stallions between 4 and 20 years of age were monitored. Total and progressive motility, viability and morphology of sperm were evaluated at time intervals of 24, 48 and 72 hours after collection. Results The total motility, progressive motility, and values of sperm with normal morphology were significantly higher in the INRA 96 and BotuSemen Gold extenders than in the skimmed milk-based extender (p < 0.01). The sperm viability differed significantly in all extenders (p < 0.01). The highest value of sperm viability was in INRA 96 (64.69 ± 0.67%) and lowest in skimmed milk-based extender (59.70 ± 0.81%). The highest differences occurred at 72 hours of storage. Values of total motility, progressive motility and sperm viability decreased over time (p < 0.01)...
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2018
The objective of the performed experiment was to evaluate growth performance and selected carcass... more The objective of the performed experiment was to evaluate growth performance and selected carcass traits and meat quality parameters in pigs of a native Czech breed Prestice Black-Pied pig (PB) and a commercial hybrid (H) used for meat production. The observation included 40 pigs, 20 pigs of the native breed PB and 20 pigs of the hybrid combination. The PB piglets showed higher (P ≤ 0.05) birth weight than the H piglets. The lifetime weight gain was higher (P ≤ 0.01) in the H pigs than in the PB pigs. The results also showed higher (P ≤ 0.01) backfat thickness in the PB breed than in the H pigs. Lean meat content was higher (P ≤ 0.01) in the hybrid pigs than in the PB breed. Higher (P ≤ 0.01) values of intramuscular fat content were found in the PB pigs than in the H pigs. Drip loss value was higher (P ≤ 0.01) in the H pigs by 1.3 % than in the PB pigs. The PB pigs showed more favourable (P ≤ 0.05) values of pH45,24 in comparison with the hybrid pigs.
In this field study, embryos were derived from genetically highly valuable cows excluded from bre... more In this field study, embryos were derived from genetically highly valuable cows excluded from breeding due to reproductive disorders. Cows, 5 to 10 years old, of Czech Siemmental, Holstein Dairy and Beef Cattle breeds were used as oocyte donors. Oocytes were obtained either in the growth phase of the first follicular wave from cows with synchronized oestrus or in any other phases of follicular development from cows without oestrus synchronization. The embryos were prepared by a standard protocol described previously. The mean number of usable oocytes, transferable and freezable embryos per donor, and the mean percentage of usable, transferable and freezable embryos were assessed. The results were analyzed by Student’s-t and Chi-squared tests. The embryos were frozen according to a slow freezing protocol. After thawing, they were transferred to recipients on Day 7 after oestrus. Irrespective of the breed, the mean numbers of usable oocytes and transferable and freezable embryos colle...
The objective of the study was to characterize the effect of the oestrous cycle stage on the yiel... more The objective of the study was to characterize the effect of the oestrous cycle stage on the yield, morphology and meiotic competence of porcine oocytes. A total of 46 cycling gilts, at 8.5–9 months of age, were used as oocyte donors. Their oestrous cycle was synchronized by Regumate and the onset of oestrus was checked (Day 0). The gilts were slaughtered at the early (Days 1–5), middle (6–10) and late (11–14) luteal or early (Days 15–16), middle (17–19) and late (20–21) follicular phase. Oocytes were isolated separately from medium (5–9 mm) and small (≤ 4 mm) follicles. Cumulus-oocyte complexes with dark, evenly granulated cytoplasm and at least two compact layers of cumulus cells were selected as useful for maturation. They were matured by a standard protocol, denuded from cumulus cells, fixed in glutaraldehyde, stained with 33258-Hoechst and examined by epifluorescence. The oocytes collected from small and medium follicles differed in their yield, morphology and meiotic competenc...
The present study was designed to compare the efficiency of bovine embryo production for cryopres... more The present study was designed to compare the efficiency of bovine embryo production for cryopreservation between oocytes collected from donors in the growth phase of follicular development (GPFD) and those recovered from donors in the undefined phase (UPFD). Cyclic cows, Czech Siemental or Holstein dairy breeds, 4–6 years of age, slaughtered at the local abbatoir were used. They were divided into two groups based on ovarian morphology: I. GPFD donors with ovaries corresponding to the growth phase of the first follicular wave (estrus cycle days 3–4; n = 52), and II. UPFD donors with ovaries in any other phase of follicular development (undefined estrus cycle days; n = 89). A total of 3 771 oocytes were collected and 1 134 embryos were prepared as two separate populations by standard protocol. In total 352 excellent or good quality embryos at the early, advanced or expanded blastocyst stage from both donor groups were pooled and used for cryotolerance assessment. They were frozen on ...
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2019
The aim of the study was to supplement the original knowledge about the reproduction of one of fo... more The aim of the study was to supplement the original knowledge about the reproduction of one of four species of storks (Mycteria). The storks are classified by IUCN as endangered species with different degrees of extinction and their reproduction in human care is difficult. Therefore, it is necessary to continuously supplement and deepen the knowledge of their reproduction in captivity. The subject of the thesis was the analysis of reproductive indicators of the yellow‑billed ibis (Mycteria ibis). By analyzing partial reproduction indicators, we wanted to supplement the knowledge that is needed to ensure the necessary degree of reproduction of the yellow‑billed stork in human care. The monitoring was carried out in the ZOO Zlín – Lešná in these years 2011–2014. After laying, the eggs were measured and weighed, which was repeated in several‑day intervals. In addition, hatchability, the number of reared young and the weight of the young after hatching were monitored. During the monitor...
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2017
The aim of the present study was focused on analysis of reproductive traits in the painted stork ... more The aim of the present study was focused on analysis of reproductive traits in the painted stork (Mycteria leucocephala). The analysis of partial reproductive traits was intended to complete the knowledge necessary for ensuring reproduction of the painted stork in captivity on the required level. The observation was performed in the Zoo Zlín – Lešná from 2011 to 2014. The eggs were measured and weighed after laying and then in several‑day intervals. Other observed traits were hatchability of the eggs, number of raised young birds and their weight after hatching. During whole observation period, a total of 90 eggs of the painted stork were evaluated from 12 parental pairs. The average share of fertilized eggs was 38.9 %. Average length of eggs was 68.57 mm, average width was 46.43 mm and average weight was 79.79 g. Average weight loss of eggs during their incubation was 9.87 g. Average hatchability of all the laid eggs was 27.8 %. Average hatchability of the fertilized eggs was 71.4 ...
A good functional status of cryopreserved boar spermatozoa is very important for successful ferti... more A good functional status of cryopreserved boar spermatozoa is very important for successful fertilization of porcine oocytes and in vitro embryo production. The purpose of the study was to evaluate the changes in functional status of boar spermatozoa separated from frozen-thawed semen and capacitated in vitro by caffeine. The effect of acrosome reaction development in spermatozoa on the efficiency of oocyte fertilization has been studied in boars A, B and C. Motile spermatozoa were separated by Percoll gradient, untreated (control) or treated with both 1mM and 2mM caffeine, and capacitated or co-cultured with matured oocytes. The motility, viability, chromatin and acrosome integrity, and fertilizing ability of spermatozoa were assessed. The separation significantly increased (P < 0.05) the percentage of viable spermatozoa in all tested boars and percentages of motile and acrosome intact spermatozoa in boars B and C. The capacitation significantly decreased (P < 0.05) the perce...
Significance of a short-time survival test for the prediction of quality parameters of sperm obta... more Significance of a short-time survival test for the prediction of quality parameters of sperm obtained by gradient centrifugation was evaluated from an aspect of their functional resistance. Frozen semen samples from 27 bulls before and after separation were assessed by a 120-min survival test. The evaluation of morphological examination of ejaculates was carried out using the SASMO computer program. Statistically significant interrelationship between sperm quality parameters in the initial insemination doses, the amounts of obtained sperm after separation and their functional and vital resistance in the survival test was documented.
Objective This study was focused on the influence of the skimmed milk-based extender (SM), the IN... more Objective This study was focused on the influence of the skimmed milk-based extender (SM), the INRA 96 extender and BotuSemen Gold extender on parameters of stallions' ejaculate during storage. Methods In this study, 14 stallions between 4 and 20 years of age were monitored. Total and progressive motility, viability and morphology of sperm were evaluated at time intervals of 24, 48 and 72 hours after collection. Results The total motility, progressive motility, and values of sperm with normal morphology were significantly higher in the INRA 96 and BotuSemen Gold extenders than in the skimmed milk-based extender (p < 0.01). The sperm viability differed significantly in all extenders (p < 0.01). The highest value of sperm viability was in INRA 96 (64.69 ± 0.67%) and lowest in skimmed milk-based extender (59.70 ± 0.81%). The highest differences occurred at 72 hours of storage. Values of total motility, progressive motility and sperm viability decreased over time (p < 0.01)...
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2018
The objective of the performed experiment was to evaluate growth performance and selected carcass... more The objective of the performed experiment was to evaluate growth performance and selected carcass traits and meat quality parameters in pigs of a native Czech breed Prestice Black-Pied pig (PB) and a commercial hybrid (H) used for meat production. The observation included 40 pigs, 20 pigs of the native breed PB and 20 pigs of the hybrid combination. The PB piglets showed higher (P ≤ 0.05) birth weight than the H piglets. The lifetime weight gain was higher (P ≤ 0.01) in the H pigs than in the PB pigs. The results also showed higher (P ≤ 0.01) backfat thickness in the PB breed than in the H pigs. Lean meat content was higher (P ≤ 0.01) in the hybrid pigs than in the PB breed. Higher (P ≤ 0.01) values of intramuscular fat content were found in the PB pigs than in the H pigs. Drip loss value was higher (P ≤ 0.01) in the H pigs by 1.3 % than in the PB pigs. The PB pigs showed more favourable (P ≤ 0.05) values of pH45,24 in comparison with the hybrid pigs.
In this field study, embryos were derived from genetically highly valuable cows excluded from bre... more In this field study, embryos were derived from genetically highly valuable cows excluded from breeding due to reproductive disorders. Cows, 5 to 10 years old, of Czech Siemmental, Holstein Dairy and Beef Cattle breeds were used as oocyte donors. Oocytes were obtained either in the growth phase of the first follicular wave from cows with synchronized oestrus or in any other phases of follicular development from cows without oestrus synchronization. The embryos were prepared by a standard protocol described previously. The mean number of usable oocytes, transferable and freezable embryos per donor, and the mean percentage of usable, transferable and freezable embryos were assessed. The results were analyzed by Student’s-t and Chi-squared tests. The embryos were frozen according to a slow freezing protocol. After thawing, they were transferred to recipients on Day 7 after oestrus. Irrespective of the breed, the mean numbers of usable oocytes and transferable and freezable embryos colle...
The objective of the study was to characterize the effect of the oestrous cycle stage on the yiel... more The objective of the study was to characterize the effect of the oestrous cycle stage on the yield, morphology and meiotic competence of porcine oocytes. A total of 46 cycling gilts, at 8.5–9 months of age, were used as oocyte donors. Their oestrous cycle was synchronized by Regumate and the onset of oestrus was checked (Day 0). The gilts were slaughtered at the early (Days 1–5), middle (6–10) and late (11–14) luteal or early (Days 15–16), middle (17–19) and late (20–21) follicular phase. Oocytes were isolated separately from medium (5–9 mm) and small (≤ 4 mm) follicles. Cumulus-oocyte complexes with dark, evenly granulated cytoplasm and at least two compact layers of cumulus cells were selected as useful for maturation. They were matured by a standard protocol, denuded from cumulus cells, fixed in glutaraldehyde, stained with 33258-Hoechst and examined by epifluorescence. The oocytes collected from small and medium follicles differed in their yield, morphology and meiotic competenc...
The present study was designed to compare the efficiency of bovine embryo production for cryopres... more The present study was designed to compare the efficiency of bovine embryo production for cryopreservation between oocytes collected from donors in the growth phase of follicular development (GPFD) and those recovered from donors in the undefined phase (UPFD). Cyclic cows, Czech Siemental or Holstein dairy breeds, 4–6 years of age, slaughtered at the local abbatoir were used. They were divided into two groups based on ovarian morphology: I. GPFD donors with ovaries corresponding to the growth phase of the first follicular wave (estrus cycle days 3–4; n = 52), and II. UPFD donors with ovaries in any other phase of follicular development (undefined estrus cycle days; n = 89). A total of 3 771 oocytes were collected and 1 134 embryos were prepared as two separate populations by standard protocol. In total 352 excellent or good quality embryos at the early, advanced or expanded blastocyst stage from both donor groups were pooled and used for cryotolerance assessment. They were frozen on ...
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2019
The aim of the study was to supplement the original knowledge about the reproduction of one of fo... more The aim of the study was to supplement the original knowledge about the reproduction of one of four species of storks (Mycteria). The storks are classified by IUCN as endangered species with different degrees of extinction and their reproduction in human care is difficult. Therefore, it is necessary to continuously supplement and deepen the knowledge of their reproduction in captivity. The subject of the thesis was the analysis of reproductive indicators of the yellow‑billed ibis (Mycteria ibis). By analyzing partial reproduction indicators, we wanted to supplement the knowledge that is needed to ensure the necessary degree of reproduction of the yellow‑billed stork in human care. The monitoring was carried out in the ZOO Zlín – Lešná in these years 2011–2014. After laying, the eggs were measured and weighed, which was repeated in several‑day intervals. In addition, hatchability, the number of reared young and the weight of the young after hatching were monitored. During the monitor...
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 2017
The aim of the present study was focused on analysis of reproductive traits in the painted stork ... more The aim of the present study was focused on analysis of reproductive traits in the painted stork (Mycteria leucocephala). The analysis of partial reproductive traits was intended to complete the knowledge necessary for ensuring reproduction of the painted stork in captivity on the required level. The observation was performed in the Zoo Zlín – Lešná from 2011 to 2014. The eggs were measured and weighed after laying and then in several‑day intervals. Other observed traits were hatchability of the eggs, number of raised young birds and their weight after hatching. During whole observation period, a total of 90 eggs of the painted stork were evaluated from 12 parental pairs. The average share of fertilized eggs was 38.9 %. Average length of eggs was 68.57 mm, average width was 46.43 mm and average weight was 79.79 g. Average weight loss of eggs during their incubation was 9.87 g. Average hatchability of all the laid eggs was 27.8 %. Average hatchability of the fertilized eggs was 71.4 ...
A good functional status of cryopreserved boar spermatozoa is very important for successful ferti... more A good functional status of cryopreserved boar spermatozoa is very important for successful fertilization of porcine oocytes and in vitro embryo production. The purpose of the study was to evaluate the changes in functional status of boar spermatozoa separated from frozen-thawed semen and capacitated in vitro by caffeine. The effect of acrosome reaction development in spermatozoa on the efficiency of oocyte fertilization has been studied in boars A, B and C. Motile spermatozoa were separated by Percoll gradient, untreated (control) or treated with both 1mM and 2mM caffeine, and capacitated or co-cultured with matured oocytes. The motility, viability, chromatin and acrosome integrity, and fertilizing ability of spermatozoa were assessed. The separation significantly increased (P < 0.05) the percentage of viable spermatozoa in all tested boars and percentages of motile and acrosome intact spermatozoa in boars B and C. The capacitation significantly decreased (P < 0.05) the perce...
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