To study antibody responses associated with molecular mimicry in periodontitis. Fifty-four period... more To study antibody responses associated with molecular mimicry in periodontitis. Fifty-four periodontitis cases (mean age 54.0 years) and 44 controls (53.6 years) were examined, after which cases received periodontal treatment. Established immunoassays were used to analyse levels of antibodies against two pathogens, Aggregatibacter actinomycetemcomitans (Aa) and Porphyromonas gingivalis (Pg), heat shock proteins (Hsp), Hsp60, Hsp65, and Hsp70, and epitopes of oxidized low-density lipoprotein (oxLDL) (CuOx-LDL and MDA-LDL) in plasma samples that were collected at baseline and after 3 (n = 48) and 6 (n = 30) months. When age, sex, smoking habit, and the number of teeth were considered in multivariate logistic regressions, Aa and Pg IgG, Hsp65-IgA, CuOx-LDL-IgG and -IgM, and MDA-LDL-IgG antibody levels were associated with periodontitis, whereas Hsp60-IgG2 antibody levels were inversely associated. The Aa antibody levels significantly correlated with the levels of IgA antibodies to Hsp65 and Hsp70, and both OxLDL IgA antibody levels. The levels of antibodies to Pg correlated with IgG antibodies to Hsp60, Hsp70, and both oxLDL antibody epitopes. None of the antibody levels changed significantly after treatment. Periodontitis is associated with persistently high levels of circulating antibodies that are reactive with pathogen- and host-derived antigens.
The International journal of oral & maxillofacial implants, 1989
Four mandibular specimens were radiographically examined bilaterally to locate the mandibular can... more Four mandibular specimens were radiographically examined bilaterally to locate the mandibular canal. The following radiographic techniques were used: periapical and panoramic radiography, hypocycloidal tomography, and computed tomography (CT). The distance from the crest of the alveolar process to the superior border of the mandibular canal was measured in millimeters on all radiographs. The specimens were then sectioned, and the location of the mandibular canal (as measured on contact radiographs of the sections) was compared with measurements made on the other radiographs. The results showed that CT gave the most accurate position of the mandibular canal and is therefore probably the best method for preoperative planning of the implant surgery involving the area close to the mandibular canal.
... Mustafa W, Sharafeldin A, Diab A, Huang YM, Bing H, Zhu J, Link H, Frithiof L, Klinge B. Coor... more ... Mustafa W, Sharafeldin A, Diab A, Huang YM, Bing H, Zhu J, Link H, Frithiof L, Klinge B. Coordinate Up-Regulation of the b ... Cytokines 1993;5:175–9. 18 Rollins BJ, Yoshimura T, Leonard AJ, Pober J. Cytokine activated human endothelial cells synthesize and secrete monocyte ...
... men behandlas ändå som en separat biologisk enhet såväl i socialförsäkringssystemen som i den... more ... men behandlas ändå som en separat biologisk enhet såväl i socialförsäkringssystemen som i den medicinska utbildningen, menar Björn Klinge ... Och patienterna vittnar redan nu om uppskattning och tillfreds-ställelse i det professionella mötet mel-lan tandläkare och patient. ...
Gastrectomy induces bone loss, suggesting that the stomach is important for calcium homeostasis. ... more Gastrectomy induces bone loss, suggesting that the stomach is important for calcium homeostasis. In this study we examined the effects of gastrectomy, with or without CaCl2 supplementation, on the structure of the calvaria of the rat. The calvaria were dissected out and transilluminated, and the calvaria thickness was measured before (micrometer) and after fixation and sectioning (microscopy). Sections of the skull were analysed planimetrically for bone tissue area, using computer-assisted image analysis. The time course of the gastrectomy-produced bone loss was studied. After 4 weeks the remaining bone represented about 70% of that in control rats, and after 8 weeks the value was 50%. The thickness of the calvaria was lower in gastrectomized rats than in sham-operated controls. Bone marrow and samples from liver and spleen were examined; no differences were found between experimental and control groups. Daily ingestion of 100 mg CaCl2.2H2O did not prevent the bone loss. It is unlikely that the gastrectomy-produced bone loss reflects calcium deficiency. The results rather support the view that the stomach is important for calcium homeostasis through another mechanism, perhaps involving a hypothetical gastric hormone.
The aim of this study was to describe, in the domestic ferret, the composition of the normal ging... more The aim of this study was to describe, in the domestic ferret, the composition of the normal gingival sulcus and the near related mucosa microflora and the shift in the composition during an acute periodontal breakdown induced by the placement of ligatures. Five animals were used. At the start of the experiment (day 0) and before the placement of the ligatures, 2 microbiological samples were obtained from the gingival sulcus and the related mucosa at the mid-buccal surface of the right upper P4. The same sites were sampled on the left side, serving as control sites. On day 28, new microbiological samples were taken from the same 4 sites. The samples were analyzed with darkfield microscopy and with viable counts. In the darkfield analysis, on day 0, the predominant bacterial morphotype was cocci, ranging from 88-92%. The viable counts showed that nearly 100% of the total cultivable flora was composed of facultative anaerobic gram-negative and gram-positive rods. Pasteurella spp., Corynebacterium spp. and Rothia spp. were the major components of these bacterial groups. No anaerobic bacteria were detected. On day 28, cocci decreased to approximately 75% in the experimental gingival sulcus sample, and filaments and rods increased significantly. Spirochetes remained largely unchanged during the experiment. The composition of the experimental site samples changed. There was a decrease in the mean numbers of facultative anaerobic gram-positive rods, and approximately one third of the microflora comprised anaerobic gram-negative rods, mainly Porphyromonas gingivalis and Fusobacterium spp. Although the composition of the microflora of the ferret presented few similarities to that described in dogs and monkeys, the anaerobic portion of the gingival sulcus and oral mucosa microflora related to ligature-induced periodontitis presented microorganisms observed in experimental periodontitis both in dogs and monkeys as well as in human periodontal diseases.
A safe and easy-to-manage technique for various craniotomy procedures in young rabbits has been d... more A safe and easy-to-manage technique for various craniotomy procedures in young rabbits has been developed. This technique, which minimizes the need for special instrumentation, has been tested in 90 animals with a minimal mortality and morbidity: one death perioperatively caused by sagittal sinus bleeding and one rabbit disclosing a brief period of postoperative illness, respectively. The technique, including postsurgical strategy, is described in detail.
Growing experimental evidence implicates chronic inflammation/infection due to periodontal diseas... more Growing experimental evidence implicates chronic inflammation/infection due to periodontal diseases as a risk factor for death. The objective was to evaluate the role of periodontitis in premature death in a prospective study. The causes of death in 3273 randomly-selected subjects, aged 30-40 years, from 1985 to 2001 were registered. At baseline, 1676 individuals underwent a clinical oral examination (Group A) and 1597 did not (Group B). Mortality and causes of death from 1985 to 2001 were recorded according to ICD-9-10. In Groups A (clinically examined group) and B, a total of 110 subjects had died: 40 subjects in Group A, and 70 in Group B. In Group A significant differences were present at baseline between survivors and persons who later died, with respect to dental plaque, calculus, gingival inflammation and number of missing molars in subjects with periodontitis (p < 0.001). The multiple logistic regression analysis results of the relationship between being dead (dependent variable) and several independent variables identified periodontitis with any missing molars as a principal independent predictor of death. Young individuals with periodontitis and missing molars seem to be at increased risk for premature death by life-threatening diseases, such as neoplasms, and diseases of the circulatory and digestive systems.
The aim was to validate self-perceived oral health with salivary IgG as an inflammatory parameter... more The aim was to validate self-perceived oral health with salivary IgG as an inflammatory parameter in children with type 1 diabetes. Unstimulated whole saliva samples were collected from 36 children with well controlled and 12 with poorly controlled type 1 diabetes and 40 non-diabetic children (Controls). Salivary flow rate, random blood glucose level, salivary protein concentration and immunoglobulin A and G levels were recorded using standard techniques. Data concerning oral health and diabetes status were collected. Self-perceived gingival bleeding (bleeding gums), bad breath and dry mouth were higher in diabetic children when compared with those in controls (P < 0.05). Gingival bleeding was frequently perceived by children with poorly controlled compared to well-controlled type 1 diabetes (P < 0.05) and controls (P < 0.001). Bad breath was common perceived by children with poorly controlled compared to well-controlled type 1 diabetes (P < 0.05) and controls (P < 0.0001). Salivary flow rate was lower in the diabetic children compared to controls (P < 0.01) with no difference between children with poorly controlled and well-controlled type 1 diabetes. Salivary IgG per mg protein concentration was higher in the diabetics when compared with the control group (P < 0.0001). IgG per mg protein levels were also higher in children with poorly controlled when compared with well-controlled type 1 diabetes (P < 0.05). There was no difference in IgA per mg protein and total protein concentrations between children with poorly controlled and well-controlled type 1 diabetes. Self-perceived gingival bleeding and salivary IgG per mg protein concentration were increased in children with type 1 diabetes compared with controls. These variables were also increased in children with poorly controlled compared with well-controlled type 1 diabetes.
The aim of this study was to analyse changes in bone height after 17 years in smokers and non-smo... more The aim of this study was to analyse changes in bone height after 17 years in smokers and non-smokers with periodontal disease, and to compare these with clinical assessment outcome. Participants comprised 50 adults with periodontitis and 18 healthy controls from a randomly selected epidemiological sample. Their mean age at the end of the study was 54.2 (SD+/-3.09) years. The study included radiographic analysis compared with clinical data. The periodontitis group had significantly (p<0.001) higher values than their healthy counterparts for plaque index (PLI), gingival index (GI), calculus index (CI), and bleeding on probing (BOP) at baseline and after 17 years. At the end of the follow-up, never-smokers with periodontitis had higher values for PLI (p<0.05) and ex-smokers and smokers had higher GI and BOP (p<0.001) than the controls. In all individuals with periodontitis, maxillary molars were most affected. Smokers had more severe marginal bone loss over time. Vertical bone defects were more often seen on the mesial side of teeth (p<0.05). Marginal bone level in this prospective study did reveal tooth groups at higher risk for progression of periodontal disease.
The aim of this study was to evaluate the clinical and histological characteristics of ligature i... more The aim of this study was to evaluate the clinical and histological characteristics of ligature induced periodontitis in 12 domestic ferrets. On day 0, silk ligatures were placed at the gingival margin level of selected experimental teeth. The contralateral teeth served as control teeth. On day 14, 4 animals and on day 28, 8 animals were sacrificed. Clinical measurements including gingival index (GI), probing pocket depth (PPD) and probing attachment level (PAL) were taken in 5 animals on days 0, 14 and 28. Histological sections were prepared for light microscopy. The histometric measurements performed were (1) the distance between the cemento-enamel junction (CEJ) and the alveolar bone crest and (2) the distance between CEJ and connective tissue attachment level. The number of sections with root resorption areas was also observed. Cell counts were taken in 4 different areas. Clinical results showed, on days 14 and 28, a significant increase of the mean values of PPD and PAL at the experimental teeth when compared to the control teeth. The histometric results showed a significant loss of connective tissue attachment and bone loss in the experimental teeth on day 14. On day 28, the results were similar, although more sections with root resorption areas were observed in the experimental teeth. The predominant inflammatory cell in all the 4 experimental areas analyzed was polymorphonuclears (PMN), and they were mainly increased in areas closely related to the junctional epithelium. The results indicate that the domestic ferret may be used as an experimental animal in the ligature-induced periodontitis model.
To study antibody responses associated with molecular mimicry in periodontitis. Fifty-four period... more To study antibody responses associated with molecular mimicry in periodontitis. Fifty-four periodontitis cases (mean age 54.0 years) and 44 controls (53.6 years) were examined, after which cases received periodontal treatment. Established immunoassays were used to analyse levels of antibodies against two pathogens, Aggregatibacter actinomycetemcomitans (Aa) and Porphyromonas gingivalis (Pg), heat shock proteins (Hsp), Hsp60, Hsp65, and Hsp70, and epitopes of oxidized low-density lipoprotein (oxLDL) (CuOx-LDL and MDA-LDL) in plasma samples that were collected at baseline and after 3 (n = 48) and 6 (n = 30) months. When age, sex, smoking habit, and the number of teeth were considered in multivariate logistic regressions, Aa and Pg IgG, Hsp65-IgA, CuOx-LDL-IgG and -IgM, and MDA-LDL-IgG antibody levels were associated with periodontitis, whereas Hsp60-IgG2 antibody levels were inversely associated. The Aa antibody levels significantly correlated with the levels of IgA antibodies to Hsp65 and Hsp70, and both OxLDL IgA antibody levels. The levels of antibodies to Pg correlated with IgG antibodies to Hsp60, Hsp70, and both oxLDL antibody epitopes. None of the antibody levels changed significantly after treatment. Periodontitis is associated with persistently high levels of circulating antibodies that are reactive with pathogen- and host-derived antigens.
The International journal of oral & maxillofacial implants, 1989
Four mandibular specimens were radiographically examined bilaterally to locate the mandibular can... more Four mandibular specimens were radiographically examined bilaterally to locate the mandibular canal. The following radiographic techniques were used: periapical and panoramic radiography, hypocycloidal tomography, and computed tomography (CT). The distance from the crest of the alveolar process to the superior border of the mandibular canal was measured in millimeters on all radiographs. The specimens were then sectioned, and the location of the mandibular canal (as measured on contact radiographs of the sections) was compared with measurements made on the other radiographs. The results showed that CT gave the most accurate position of the mandibular canal and is therefore probably the best method for preoperative planning of the implant surgery involving the area close to the mandibular canal.
... Mustafa W, Sharafeldin A, Diab A, Huang YM, Bing H, Zhu J, Link H, Frithiof L, Klinge B. Coor... more ... Mustafa W, Sharafeldin A, Diab A, Huang YM, Bing H, Zhu J, Link H, Frithiof L, Klinge B. Coordinate Up-Regulation of the b ... Cytokines 1993;5:175–9. 18 Rollins BJ, Yoshimura T, Leonard AJ, Pober J. Cytokine activated human endothelial cells synthesize and secrete monocyte ...
... men behandlas ändå som en separat biologisk enhet såväl i socialförsäkringssystemen som i den... more ... men behandlas ändå som en separat biologisk enhet såväl i socialförsäkringssystemen som i den medicinska utbildningen, menar Björn Klinge ... Och patienterna vittnar redan nu om uppskattning och tillfreds-ställelse i det professionella mötet mel-lan tandläkare och patient. ...
Gastrectomy induces bone loss, suggesting that the stomach is important for calcium homeostasis. ... more Gastrectomy induces bone loss, suggesting that the stomach is important for calcium homeostasis. In this study we examined the effects of gastrectomy, with or without CaCl2 supplementation, on the structure of the calvaria of the rat. The calvaria were dissected out and transilluminated, and the calvaria thickness was measured before (micrometer) and after fixation and sectioning (microscopy). Sections of the skull were analysed planimetrically for bone tissue area, using computer-assisted image analysis. The time course of the gastrectomy-produced bone loss was studied. After 4 weeks the remaining bone represented about 70% of that in control rats, and after 8 weeks the value was 50%. The thickness of the calvaria was lower in gastrectomized rats than in sham-operated controls. Bone marrow and samples from liver and spleen were examined; no differences were found between experimental and control groups. Daily ingestion of 100 mg CaCl2.2H2O did not prevent the bone loss. It is unlikely that the gastrectomy-produced bone loss reflects calcium deficiency. The results rather support the view that the stomach is important for calcium homeostasis through another mechanism, perhaps involving a hypothetical gastric hormone.
The aim of this study was to describe, in the domestic ferret, the composition of the normal ging... more The aim of this study was to describe, in the domestic ferret, the composition of the normal gingival sulcus and the near related mucosa microflora and the shift in the composition during an acute periodontal breakdown induced by the placement of ligatures. Five animals were used. At the start of the experiment (day 0) and before the placement of the ligatures, 2 microbiological samples were obtained from the gingival sulcus and the related mucosa at the mid-buccal surface of the right upper P4. The same sites were sampled on the left side, serving as control sites. On day 28, new microbiological samples were taken from the same 4 sites. The samples were analyzed with darkfield microscopy and with viable counts. In the darkfield analysis, on day 0, the predominant bacterial morphotype was cocci, ranging from 88-92%. The viable counts showed that nearly 100% of the total cultivable flora was composed of facultative anaerobic gram-negative and gram-positive rods. Pasteurella spp., Corynebacterium spp. and Rothia spp. were the major components of these bacterial groups. No anaerobic bacteria were detected. On day 28, cocci decreased to approximately 75% in the experimental gingival sulcus sample, and filaments and rods increased significantly. Spirochetes remained largely unchanged during the experiment. The composition of the experimental site samples changed. There was a decrease in the mean numbers of facultative anaerobic gram-positive rods, and approximately one third of the microflora comprised anaerobic gram-negative rods, mainly Porphyromonas gingivalis and Fusobacterium spp. Although the composition of the microflora of the ferret presented few similarities to that described in dogs and monkeys, the anaerobic portion of the gingival sulcus and oral mucosa microflora related to ligature-induced periodontitis presented microorganisms observed in experimental periodontitis both in dogs and monkeys as well as in human periodontal diseases.
A safe and easy-to-manage technique for various craniotomy procedures in young rabbits has been d... more A safe and easy-to-manage technique for various craniotomy procedures in young rabbits has been developed. This technique, which minimizes the need for special instrumentation, has been tested in 90 animals with a minimal mortality and morbidity: one death perioperatively caused by sagittal sinus bleeding and one rabbit disclosing a brief period of postoperative illness, respectively. The technique, including postsurgical strategy, is described in detail.
Growing experimental evidence implicates chronic inflammation/infection due to periodontal diseas... more Growing experimental evidence implicates chronic inflammation/infection due to periodontal diseases as a risk factor for death. The objective was to evaluate the role of periodontitis in premature death in a prospective study. The causes of death in 3273 randomly-selected subjects, aged 30-40 years, from 1985 to 2001 were registered. At baseline, 1676 individuals underwent a clinical oral examination (Group A) and 1597 did not (Group B). Mortality and causes of death from 1985 to 2001 were recorded according to ICD-9-10. In Groups A (clinically examined group) and B, a total of 110 subjects had died: 40 subjects in Group A, and 70 in Group B. In Group A significant differences were present at baseline between survivors and persons who later died, with respect to dental plaque, calculus, gingival inflammation and number of missing molars in subjects with periodontitis (p < 0.001). The multiple logistic regression analysis results of the relationship between being dead (dependent variable) and several independent variables identified periodontitis with any missing molars as a principal independent predictor of death. Young individuals with periodontitis and missing molars seem to be at increased risk for premature death by life-threatening diseases, such as neoplasms, and diseases of the circulatory and digestive systems.
The aim was to validate self-perceived oral health with salivary IgG as an inflammatory parameter... more The aim was to validate self-perceived oral health with salivary IgG as an inflammatory parameter in children with type 1 diabetes. Unstimulated whole saliva samples were collected from 36 children with well controlled and 12 with poorly controlled type 1 diabetes and 40 non-diabetic children (Controls). Salivary flow rate, random blood glucose level, salivary protein concentration and immunoglobulin A and G levels were recorded using standard techniques. Data concerning oral health and diabetes status were collected. Self-perceived gingival bleeding (bleeding gums), bad breath and dry mouth were higher in diabetic children when compared with those in controls (P < 0.05). Gingival bleeding was frequently perceived by children with poorly controlled compared to well-controlled type 1 diabetes (P < 0.05) and controls (P < 0.001). Bad breath was common perceived by children with poorly controlled compared to well-controlled type 1 diabetes (P < 0.05) and controls (P < 0.0001). Salivary flow rate was lower in the diabetic children compared to controls (P < 0.01) with no difference between children with poorly controlled and well-controlled type 1 diabetes. Salivary IgG per mg protein concentration was higher in the diabetics when compared with the control group (P < 0.0001). IgG per mg protein levels were also higher in children with poorly controlled when compared with well-controlled type 1 diabetes (P < 0.05). There was no difference in IgA per mg protein and total protein concentrations between children with poorly controlled and well-controlled type 1 diabetes. Self-perceived gingival bleeding and salivary IgG per mg protein concentration were increased in children with type 1 diabetes compared with controls. These variables were also increased in children with poorly controlled compared with well-controlled type 1 diabetes.
The aim of this study was to analyse changes in bone height after 17 years in smokers and non-smo... more The aim of this study was to analyse changes in bone height after 17 years in smokers and non-smokers with periodontal disease, and to compare these with clinical assessment outcome. Participants comprised 50 adults with periodontitis and 18 healthy controls from a randomly selected epidemiological sample. Their mean age at the end of the study was 54.2 (SD+/-3.09) years. The study included radiographic analysis compared with clinical data. The periodontitis group had significantly (p<0.001) higher values than their healthy counterparts for plaque index (PLI), gingival index (GI), calculus index (CI), and bleeding on probing (BOP) at baseline and after 17 years. At the end of the follow-up, never-smokers with periodontitis had higher values for PLI (p<0.05) and ex-smokers and smokers had higher GI and BOP (p<0.001) than the controls. In all individuals with periodontitis, maxillary molars were most affected. Smokers had more severe marginal bone loss over time. Vertical bone defects were more often seen on the mesial side of teeth (p<0.05). Marginal bone level in this prospective study did reveal tooth groups at higher risk for progression of periodontal disease.
The aim of this study was to evaluate the clinical and histological characteristics of ligature i... more The aim of this study was to evaluate the clinical and histological characteristics of ligature induced periodontitis in 12 domestic ferrets. On day 0, silk ligatures were placed at the gingival margin level of selected experimental teeth. The contralateral teeth served as control teeth. On day 14, 4 animals and on day 28, 8 animals were sacrificed. Clinical measurements including gingival index (GI), probing pocket depth (PPD) and probing attachment level (PAL) were taken in 5 animals on days 0, 14 and 28. Histological sections were prepared for light microscopy. The histometric measurements performed were (1) the distance between the cemento-enamel junction (CEJ) and the alveolar bone crest and (2) the distance between CEJ and connective tissue attachment level. The number of sections with root resorption areas was also observed. Cell counts were taken in 4 different areas. Clinical results showed, on days 14 and 28, a significant increase of the mean values of PPD and PAL at the experimental teeth when compared to the control teeth. The histometric results showed a significant loss of connective tissue attachment and bone loss in the experimental teeth on day 14. On day 28, the results were similar, although more sections with root resorption areas were observed in the experimental teeth. The predominant inflammatory cell in all the 4 experimental areas analyzed was polymorphonuclears (PMN), and they were mainly increased in areas closely related to the junctional epithelium. The results indicate that the domestic ferret may be used as an experimental animal in the ligature-induced periodontitis model.
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