(Q40150009)

17 September 2017

title

An optimized protocol for protein purification in cultured mammalian cells using a tandem affinity purification approach (English)

1 reference

17 September 2017

Arthur Tsai

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17 September 2017

Russ P Carstens

series ordinal

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17 September 2017

publication date

1 January 2006

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17 September 2017

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17 September 2017

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17 September 2017

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17 September 2017

page(s)

2820-2827

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A rapid and efficient protocol to purify biologically active recombinant proteins from mammalian cells.

17 September 2017

cites work

1 reference

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The tandem affinity purification (TAP) method: a general procedure of protein complex purification

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An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells

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Highly efficient tandem affinity purification of trypanosome protein complexes based on a novel epitope combination

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Biochemical analysis of the EJC reveals two new factors and a stable tetrameric protein core

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Comprehensive proteomic analysis of human Par protein complexes reveals an interconnected protein network

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Identification of novel protein-protein interactions using a versatile mammalian tandem affinity purification expression system

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A systematic comparison of relative promoter/enhancer activities in mammalian cell lines

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Two methods for improved purification of full-length mammalian proteins that have poor expression and/or solubility using standard Escherichia coli procedures.

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The novel mechanism of initiation of picornavirus RNA translation

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Proteomic Analysis of SRm160-containing Complexes Reveals a Conserved Association with Cohesin

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Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei

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Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency

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A segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation

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inferred from DOI database lookup

7 January 2021

based on heuristic

Identifiers

DOI

10.1038/NPROT.2006.371

1 reference

17 September 2017

1 reference