(Q33638891)

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Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli

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Europe PubMed Central

PubMed publication ID

28 July 2017

Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli (English)

1 reference

Europe PubMed Central

PubMed publication ID

28 July 2017

Escherichia coli

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Robert E. Bruccoleri

8

object named as

R E Bruccoleri

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Europe PubMed Central

PubMed publication ID

28 July 2017

Meredith Mudgett-Hunter

3

object named as

M Mudgett-Hunter

1 reference

Europe PubMed Central

PubMed publication ID

28 July 2017

Jiří Novotný

5

object named as

J Novotný

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Europe PubMed Central

PubMed publication ID

28 July 2017

author name string

J S Huston

1

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Europe PubMed Central

PubMed publication ID

28 July 2017

D Levinson

2

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Europe PubMed Central

PubMed publication ID

28 July 2017

M S Tai

4

1 reference

Europe PubMed Central

PubMed publication ID

28 July 2017

M N Margolies

6

1 reference

Europe PubMed Central

PubMed publication ID

28 July 2017

R J Ridge

7

1 reference

Europe PubMed Central

PubMed publication ID

28 July 2017

E Haber

9

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Europe PubMed Central

PubMed publication ID

28 July 2017

R Crea

10

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Europe PubMed Central

PubMed publication ID

28 July 2017

language of work or name

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publication date

1 August 1988

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Europe PubMed Central

PubMed publication ID

28 July 2017

number of pages

5

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Proceedings of the National Academy of Sciences of the United States of America

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Europe PubMed Central

PubMed publication ID

28 July 2017

85

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Europe PubMed Central

PubMed publication ID

28 July 2017

16

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Europe PubMed Central

PubMed publication ID

28 July 2017

5879-5883

1 reference

Europe PubMed Central

PubMed publication ID

28 July 2017

Protein measurement with the Folin phenol reagent

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

RECOVERY OF SPECIFIC ACTIVITY AFTER COMPLETE UNFOLDING AND REDUCTION OF AN ANTIBODY FRAGMENT

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

RECOVERY OF ANTIGENIC SPECIFICITY AFTER DENATURATION AND COMPLETE REDUCTION OF DISULFIDES IN A PAPAIN FRAGMENT OF ANTIBODY

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Generation of antibody activity from immunoglobulin polypeptide chains produced in Escherichia coli

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Assembly of functional antibodies from immunoglobulin heavy and light chains synthesised inE. coli

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Numbers of Receptor Sites from Scatchard Graphs: Facts and Fantasies

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Codon frequencies in 119 individual genes confirm consistent choices of degenerate bases according to genome type.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

1 Measurement of molecular weights by electrophoresis on SDS-acrylamide gel

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Properties of the Fd fragment from rabbit immunoglobulin G

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Active antibody fragment (Fv) composed of the variable portions of heavy and light chains

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Localization of antibody-combining sites within the variable portions of heavy and light chains

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

An Active Derivative of Rabbit Antibody Light Chain Composed of the Constant and the Variable Domains Held Together Only by a Native Disulfide Bond

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Specific cleavage between variable and constant domains of rabbit antibody light chains by dilute acid hydrolysis.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Binding and structural diversity among high-affinity monoclonal anti-digoxin antibodies.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

The synthesis and in vivo assembly of functional antibodies in yeast.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Replacing the complementarity-determining regions in a human antibody with those from a mouse

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Variable region framework differences result in decreased or increased affinity of variant anti-digoxin antibodies

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Phosphocholine binding immunoglobulin Fab McPC603. An X-ray diffraction study at 2.7 A

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Site-directed mutagenesis of an invariant amino acid residue at the variable-diversity segments junction of an antibody

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Chemical synthesis and expression of a calmodulin gene designed for site-specific mutagenesis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

The Protein Data Bank: a computer-based archival file for macromolecular structures

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Analysis of the repertoire of anti-(4-hydroxy-3-nitro-phenyl)acetyl (NP) antibodies in C 57 BL/6 mice by cell fusion. II. Characterization of idiotopes by monoclonal anti-idiotope antibodies

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Cold pepsin digestion: a novel method to produce the Fv fragment from human immunoglobulin M

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Translation of the leader region of the Escherichia coli tryptophan operon

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

DNA sequencing with chain-terminating inhibitors

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Immunogenicity and kinetics of distribution and elimination of sheep digoxin-specific IgG and Fab fragments in the rabbit and baboon

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

Preparation of Fv fragment from the mouse myeloma XRPC-25 immunoglobulin possessing antidinitrophenyl activity

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

21 June 2018

The hydrolysis of rabbit y-globulin and antibodies with crystalline papain

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

30 October 2018

Characterization of the Fv fragment isolated from a human immunoglobulin M.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=281868

30 October 2018

Folding and interaction of subunits at the antibody combining site

1 reference

https://pubmed.ncbi.nlm.nih.gov/3045807

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Preliminary refinement and structural analysis of the Fab fragment from human immunoglobulin new at 2.0 A resolution

1 reference

https://pubmed.ncbi.nlm.nih.gov/3045807

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Crystallographic refinement and atomic models of the intact immunoglobulin molecule Kol and its antigen-binding fragment at 3.0 A and 1.0 A resolution

1 reference

https://pubmed.ncbi.nlm.nih.gov/3045807

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

A gas-liquid solid phase peptide and protein sequenator

1 reference

https://pubmed.ncbi.nlm.nih.gov/3045807

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

High-affinity monoclonal antibodies to the cardiac glycoside, digoxin

1 reference

https://pubmed.ncbi.nlm.nih.gov/3045807

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Rapid analysis of amino acids using pre-column derivatization

1 reference

https://pubmed.ncbi.nlm.nih.gov/3045807

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Prediction of the folding of short polypeptide segments by uniform conformational sampling

1 reference

https://pubmed.ncbi.nlm.nih.gov/3045807

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Immunoglobulin chain recombination among antidigoxin antibodies by hybridoma-hybridoma fusion

1 reference

https://pubmed.ncbi.nlm.nih.gov/3045807

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Proteolytic dissection of a hapten binding site

1 reference

https://pubmed.ncbi.nlm.nih.gov/3045807

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Characterization of antibodies of high affinity and specificity for the digitalis glycoside digoxin

1 reference

https://pubmed.ncbi.nlm.nih.gov/3045807

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Anomalous cleavage of aspartyl-proline peptide bonds during amino acid sequence determinations

1 reference

https://pubmed.ncbi.nlm.nih.gov/3045807

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1073/PNAS.85.16.5879

1 reference

Europe PubMed Central

PubMed publication ID

28 July 2017

1988PNAS...85.5879H

0 references

PMC publication ID

1 reference

Europe PubMed Central

PubMed publication ID

28 July 2017

PubMed publication ID

1 reference

Europe PubMed Central

PubMed publication ID

28 July 2017

ResearchGate publication ID

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