(Q30974237)

27 June 2017

title

Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display (English)

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27 June 2017

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Hanes J

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27 June 2017

Schaffitzel C

series ordinal

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27 June 2017

Knappik A

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27 June 2017

Plückthun A

series ordinal

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27 June 2017

publication date

1 December 2000

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27 June 2017

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27 June 2017

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27 June 2017

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27 June 2017

page(s)

1287-1292

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Ribosome display: an in vitro method for selection and evolution of antibodies from libraries

27 June 2017

cites work

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7 January 2021

Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface

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7 January 2021

15 Libraries of peptides and proteins displayed on filamentous phage

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7 January 2021

Making antibodies by phage display technology

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7 January 2021

An in vitro polysome display system for identifying ligands from very large peptide libraries

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7 January 2021

In vitro selection and evolution of functional proteins by using ribosome display

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7 January 2021

Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries.

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7 January 2021

RNA-peptide fusions for the in vitro selection of peptides and proteins

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7 January 2021

In vitro virus: bonding of mRNA bearing puromycin at the 3'-terminal end to the C-terminal end of its encoded protein on the ribosome in vitro

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7 January 2021

Selection of a human anti-progesterone antibody fragment from a transgenic mouse library by ARM ribosome display

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7 January 2021

Mimicking somatic hypermutation: affinity maturation of antibodies displayed on bacteriophage using a bacterial mutator strain

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7 January 2021

CDR walking mutagenesis for the affinity maturation of a potent human anti-HIV-1 antibody into the picomolar range

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7 January 2021

Strategies for the in vitro evolution of protein function: enzyme evolution by random recombination of improved sequences

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Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides

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7 January 2021

Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis

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Partial resistance of nascent polypeptide chains to proteolytic digestion due to ribosomal shielding

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Interaction of secreted nascent chains with surrounding membrane in Bacillus subtilis

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7 January 2021

Diversity of oligonucleotide functions

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7 January 2021

Disrupting the hydrophobic patches at the antibody variable/constant domain interface: improved in vivo folding and physical characterization of an engineered scFv fragment.

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7 January 2021

A natural antibody missing a cysteine in VH: consequences for thermodynamic stability and folding.

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7 January 2021

Antibody scFv fragments without disulfide bonds made by molecular evolution

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7 January 2021

Competition BIAcore for measuring true affinities: large differences from values determined from binding kinetics.

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Real-time competitive kinetic analysis of interactions between low-molecular-weight ligands in solution and surface-immobilized receptors

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7 January 2021

Use of surface plasmon resonance to probe the equilibrium and dynamic aspects of interactions between biological macromolecules.

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7 January 2021

Rapid evolution of a protein in vitro by DNA shuffling

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7 January 2021

Randomization of genes by PCR mutagenesis

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7 January 2021

Molecular evolution by staggered extension process (StEP) in vitro recombination

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7 January 2021

Co-selection of cognate antibody-antigen pairs by selectively-infective phages.

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7 January 2021

Use of T7 RNA polymerase to direct expression of cloned genes

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7 January 2021

Parameters affecting transcription termination by Escherichia coli RNA polymerase. I. Analysis of 13 rho-independent terminators

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7 January 2021

In vitro transcription: preparative RNA yields in analytical scale reactions

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Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system

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Selection for a periplasmic factor improving phage display and functional periplasmic expression

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7 January 2021

Identifiers

DOI

10.1038/82407

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Dimensions Publication ID

27 June 2017

1011912944

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