(Q28241142)

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Enzymatic assembly of DNA molecules up to several hundred kilobases

scientific article (publication date: May 2009)

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scholarly article

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Enzymatic assembly of DNA molecules up to several hundred kilobases (English)

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genome assembly

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4

object named as

J Craig Venter

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6

object named as

Hamilton O Smith

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Clyde A Hutchison

5

object named as

Clyde A Hutchison

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Ray-Yuan Chuang

3

object named as

Ray-Yuan Chuang

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author name string

Daniel G Gibson

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Lei Young

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language of work or name

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publication date

May 2009

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6

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5

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343-5

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Ligation-independent cloning of PCR products (LIC-PCR)

1 reference

https://pubmed.ncbi.nlm.nih.gov/19363495

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New plasmid vectors for specific gene targeting in Spiroplasma citri

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https://pubmed.ncbi.nlm.nih.gov/19363495

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Designing biological systems

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Properties of overexpressed phage T5 D15 exonuclease. Similarities with Escherichia coli DNA polymerase I 5'-3' exonuclease.

1 reference

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Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome

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USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products

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Formation of covalent circles of lambda DNA by E. coli extracts

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Gene synthesis by circular assembly amplification.

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A restriction enzyme from Hemophilus influenzae. I. Purification and general properties

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Cell-free cloning using phi29 DNA polymerase

1 reference

https://pubmed.ncbi.nlm.nih.gov/19363495

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Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction

1 reference

https://pubmed.ncbi.nlm.nih.gov/19363495

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Transformation ofEscherichia coliwith large DNA molecules by electroporation

1 reference

https://pubmed.ncbi.nlm.nih.gov/19363495

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Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing

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Engineering BioBrick vectors from BioBrick parts

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Foundations for engineering biology

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Systematic assembly of a full-length infectious cDNA of mouse hepatitis virus strain A59.

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PCR-mediated recombination and mutagenesis. SOEing together tailor-made genes

1 reference

https://pubmed.ncbi.nlm.nih.gov/19363495

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Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC.

1 reference

https://pubmed.ncbi.nlm.nih.gov/19363495

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