Cytotoxic T lymphocyte antigen-4 (CTLA-4) has been identified as an immunosuppressive molecule in... more Cytotoxic T lymphocyte antigen-4 (CTLA-4) has been identified as an immunosuppressive molecule involved in the negative regulation of T cells. It is highly expressed in several types of autoimmune diseases and cancers including colorectal cancer (CRC). (1) Objective: To explore the association between CTLA-4 single nucleotide polymorphisms (SNP) and risk to (CRC) in the Saudi population. (2) Methods: In this case-control study, 100 patients with CRC and 100 matched healthy controls were genotyped for three CTLA-4 SNPs: rs11571317 (−658C > T), rs231775 (+49A > G) and rs3087243 (CT60 G > A), using TaqMan assay method. Associations were evaluated using odds ratios (ORs) and 95% confidence intervals (95% CIs) for five inheritance models (co-dominant, dominant, recessive, over-dominant and log-additive). Furthermore, CTLA-4 expression levels were evaluated using quantitative real-time PCR (Q-RT-PCR) in colon cancer and adjacent colon tissues. (3) Results: Our result showed a sig...
Checkpoint programmed death-1 (PD-1) has been identified as an immunosuppressive molecule implica... more Checkpoint programmed death-1 (PD-1) has been identified as an immunosuppressive molecule implicated in the immune evasion of transformed cells. It is highly expressed in tumor cells in order to evade host immunosurveillance. In this study, we aimed to assess the association between single nucleotide polymorphisms (SNP) of PD-1 and the risk of colorectal cancer (CRC) in the Saudi population. For this case-control study, the TaqMan assay method was used for genotyping three SNPs in the PD-1 gene in 100 CRC patients and 100 healthy controls. Associations were estimated using odds ratios (ORs) and 95% confidence intervals (95% CIs) for multiple inheritance models (codominant, dominant, recessive, over-dominant, and log-additive). Moreover, PD-1 gene expression levels were evaluated using quantitative real-time PCR in colon cancer tissue and adjacent colon tissues. We found that the PD-1 rs10204525 A allele was associated with an increased risk of developing CRC (OR = 2.35; p = 0.00657)...
Fig. 4. Maximum likelihood phylogenetic tree based on the SSU rDNA sequences showing the position... more Fig. 4. Maximum likelihood phylogenetic tree based on the SSU rDNA sequences showing the position of Myxobolus opsaridiumi sp. nov. (in bold) and related species. Accession numbers and infected tissues are listed adjacent to the species names. Numbers at the nodes represent Bayesian posterior probabilities and ML bootstrap percentages. Kudoa thyrsites (Gilchrist, 1924) was used as the outgroup.
Fig. 1. Photomicrographs of Myxobolus opsaridiumi sp. nov. infecting skin, muscle and spleen of O... more Fig. 1. Photomicrographs of Myxobolus opsaridiumi sp. nov. infecting skin, muscle and spleen of Opsaridium ubangiensis (Pellegrin, 1901). A. Fresh myxospores in frontal view. B. Fresh myxospore in lateral view. C. Giemsa-stained myxospores. D. Diagrammatic drawing of a mature myxospore.
Environmental Science and Pollution Research, 2021
The toxicity of copper, cadmium, and dieldrin in adult Gammarus locusta (a marine amphipod) is cu... more The toxicity of copper, cadmium, and dieldrin in adult Gammarus locusta (a marine amphipod) is currently unclear. Thus, G. locusta from the North Lake of Tunis were subjected to acute toxicity tests to assess LC50s at 48–96 h and to biomarker response tests through the assessment of catalase and acetylcholinesterase activities and malondialdehyde levels. The present study demonstrated the abilities of a chlorinated hydrocarbon pesticide (dieldrin) induce to oxidative stress and neurotoxicity. The comparison of metal toxicity showed that G. locusta was more sensitive to cadmium than copper. The three stressors caused significant inductions of all three biomarkers in a concentration-dependent manner. Catalase induction was dependent on exposure duration for all pollutants, while only copper led to increased malondialdehyde with longer exposure times. Catalase induction and malondialdehyde increase appeared to be sex dependent for all three pollutants. The neurotoxic effects of the pollutants were concentration dependent according to inhibition of acetylcholinesterase activity. In conclusion, catalase, malondialdehyde, and acetylcholinesterase are efficient biomarkers of copper, cadmium, and dieldrin in G. locusta.
Background: Although MAIT cells regulate the pathogenesis of various inflammatory diseases, their... more Background: Although MAIT cells regulate the pathogenesis of various inflammatory diseases, their roles in the development of colorectal cancer are still unclear. The objectives of this study is to investigate the level of circulating MAIT cells and the level of expression of the membranous KIRs receptors among CRC patients and control subjects. Peripheral blood mononuclear cells (PBMCs) were isolate and of MAIT cells were phenotypically identified by flow cytometry using various monoclonal antibodies. The presence of HLA-C1 and HLA-C2 groups were typed by PCR. Results: The result showed that the percentage of MAIT cells was slightly higher in the control subjects than in in all stages of CRC patients. Stratification analysis of 46 CRC patients indicated that the percentages of circulating MAIT cells were lower in stage II and higher in stages III, and IV. A higher frequency of HLA - C2 among patients with CRC (87%) compared with control subjects (74.4%) was detected. The protein ex...
Physical inactivity has increased in prevalence among adults in industrialized and developing cou... more Physical inactivity has increased in prevalence among adults in industrialized and developing countries owing to the fact that the majority of job situations require individuals to remain seated for extended periods of time. This research aims to evaluate the influence of cycling on a stationary bike while executing a keyboard assembly task on the task completion time, error percentage, and physiological and subjective measurements. The physiological measures were electroencephalography (EEG) and electrocardiographic (ECG) signal responses, whereas the subjective measures were subjective workload ratings and subjective body discomforts. Two variables were evaluated, namely assembly methods (with versus without pedal exercises at a moderate intensity) and session testing (pre- versus post-test). Thus, the repeated measures design (i.e., assembly method by session testing of participants) was used. According to the completion time, error %, participant self-reports, and ECG and EEG st...
Figure 2. Novomitchellia bursaelongata Harrath, Sluys & Riutort, sp. nov. Holotype. A, photograph... more Figure 2. Novomitchellia bursaelongata Harrath, Sluys & Riutort, sp. nov. Holotype. A, photograph of preserved specimen in dorsal view; some protuberances are seen on the head (arrowheads); arrow indicates the tail (T). B, photograph of preserved specimen in ventral view; arrow indicates the pharynx (PH). Scale bars not available.
Figure 1. Bayesian tree inferred from the 18S rDNA sequences showing the relationship of the new ... more Figure 1. Bayesian tree inferred from the 18S rDNA sequences showing the relationship of the new Novomitchellia species to other Tricladida species included in this analysis. Maximum likelihood (ML) yielded the same topology. Asterisks at nodes indicate posterior probabilities = 1/bootstrap values> 75% obtained respectively in the ML and Bayesian inference analyses. Scale bar: number of substitutions per nucleotide position.
New benzimidazolium salts were synthesized as <i>N</i>-heterocyclic carbene precursor... more New benzimidazolium salts were synthesized as <i>N</i>-heterocyclic carbene precursors. These NHC precursors were metallated with Cu<sub>2</sub>O and CuI in acetone and water under reflux to give novel copper(I) complexes. The structures of these benzimidazolium salts and copper(I) complexes were characterized on the basis of elemental analysis, <sup>1</sup>H NMR, <sup>13</sup>C NMR, IR and LC–MS spectroscopic techniques. The (NHC)Cu(I) complexes <b>3–4</b> were tested against <i>MCF7</i> and <i>MDA-MB-231</i> cancer cells, <i>Escherichia coli</i>, methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and <i>Candida albican</i>s microorganisms, <i>Leishmania major</i> promastigotes and amastigotes, <i>Toxoplasma gondii</i> parasites and against Vero cell line <i>in vitro</i>. The synthesized copper NHC carbene complex <b&g...
Figure 3. Seasonal variation of the prevalence of Ceratomyxa ghannouchensis n. sp. and Ceratomyxa... more Figure 3. Seasonal variation of the prevalence of Ceratomyxa ghannouchensis n. sp. and Ceratomyxa pallida from Boops boops in the Gulf of Gabès.
This dataset contains the digitized treatments in Plazi based on the original journal article Tha... more This dataset contains the digitized treatments in Plazi based on the original journal article Thabet, Aouatef, Abdel-Baki, Abdel-Azeem S., Harrath, Abdel Halim, Mansour, Lamjed (2019): Morphological and molecular aspects of Ceratomyxa ghannouchensis n. sp. and C. pallida Thélohan 1894 infecting the bogueı Boops boops (l.). JOURNAL OF NATURAL HISTORY 53: 541-556, DOI: 10.1080/00222933.2019.1597202
Cytotoxic T lymphocyte antigen-4 (CTLA-4) has been identified as an immunosuppressive molecule in... more Cytotoxic T lymphocyte antigen-4 (CTLA-4) has been identified as an immunosuppressive molecule involved in the negative regulation of T cells. It is highly expressed in several types of autoimmune diseases and cancers including colorectal cancer (CRC). (1) Objective: To explore the association between CTLA-4 single nucleotide polymorphisms (SNP) and risk to (CRC) in the Saudi population. (2) Methods: In this case-control study, 100 patients with CRC and 100 matched healthy controls were genotyped for three CTLA-4 SNPs: rs11571317 (−658C > T), rs231775 (+49A > G) and rs3087243 (CT60 G > A), using TaqMan assay method. Associations were evaluated using odds ratios (ORs) and 95% confidence intervals (95% CIs) for five inheritance models (co-dominant, dominant, recessive, over-dominant and log-additive). Furthermore, CTLA-4 expression levels were evaluated using quantitative real-time PCR (Q-RT-PCR) in colon cancer and adjacent colon tissues. (3) Results: Our result showed a sig...
Checkpoint programmed death-1 (PD-1) has been identified as an immunosuppressive molecule implica... more Checkpoint programmed death-1 (PD-1) has been identified as an immunosuppressive molecule implicated in the immune evasion of transformed cells. It is highly expressed in tumor cells in order to evade host immunosurveillance. In this study, we aimed to assess the association between single nucleotide polymorphisms (SNP) of PD-1 and the risk of colorectal cancer (CRC) in the Saudi population. For this case-control study, the TaqMan assay method was used for genotyping three SNPs in the PD-1 gene in 100 CRC patients and 100 healthy controls. Associations were estimated using odds ratios (ORs) and 95% confidence intervals (95% CIs) for multiple inheritance models (codominant, dominant, recessive, over-dominant, and log-additive). Moreover, PD-1 gene expression levels were evaluated using quantitative real-time PCR in colon cancer tissue and adjacent colon tissues. We found that the PD-1 rs10204525 A allele was associated with an increased risk of developing CRC (OR = 2.35; p = 0.00657)...
Fig. 4. Maximum likelihood phylogenetic tree based on the SSU rDNA sequences showing the position... more Fig. 4. Maximum likelihood phylogenetic tree based on the SSU rDNA sequences showing the position of Myxobolus opsaridiumi sp. nov. (in bold) and related species. Accession numbers and infected tissues are listed adjacent to the species names. Numbers at the nodes represent Bayesian posterior probabilities and ML bootstrap percentages. Kudoa thyrsites (Gilchrist, 1924) was used as the outgroup.
Fig. 1. Photomicrographs of Myxobolus opsaridiumi sp. nov. infecting skin, muscle and spleen of O... more Fig. 1. Photomicrographs of Myxobolus opsaridiumi sp. nov. infecting skin, muscle and spleen of Opsaridium ubangiensis (Pellegrin, 1901). A. Fresh myxospores in frontal view. B. Fresh myxospore in lateral view. C. Giemsa-stained myxospores. D. Diagrammatic drawing of a mature myxospore.
Environmental Science and Pollution Research, 2021
The toxicity of copper, cadmium, and dieldrin in adult Gammarus locusta (a marine amphipod) is cu... more The toxicity of copper, cadmium, and dieldrin in adult Gammarus locusta (a marine amphipod) is currently unclear. Thus, G. locusta from the North Lake of Tunis were subjected to acute toxicity tests to assess LC50s at 48–96 h and to biomarker response tests through the assessment of catalase and acetylcholinesterase activities and malondialdehyde levels. The present study demonstrated the abilities of a chlorinated hydrocarbon pesticide (dieldrin) induce to oxidative stress and neurotoxicity. The comparison of metal toxicity showed that G. locusta was more sensitive to cadmium than copper. The three stressors caused significant inductions of all three biomarkers in a concentration-dependent manner. Catalase induction was dependent on exposure duration for all pollutants, while only copper led to increased malondialdehyde with longer exposure times. Catalase induction and malondialdehyde increase appeared to be sex dependent for all three pollutants. The neurotoxic effects of the pollutants were concentration dependent according to inhibition of acetylcholinesterase activity. In conclusion, catalase, malondialdehyde, and acetylcholinesterase are efficient biomarkers of copper, cadmium, and dieldrin in G. locusta.
Background: Although MAIT cells regulate the pathogenesis of various inflammatory diseases, their... more Background: Although MAIT cells regulate the pathogenesis of various inflammatory diseases, their roles in the development of colorectal cancer are still unclear. The objectives of this study is to investigate the level of circulating MAIT cells and the level of expression of the membranous KIRs receptors among CRC patients and control subjects. Peripheral blood mononuclear cells (PBMCs) were isolate and of MAIT cells were phenotypically identified by flow cytometry using various monoclonal antibodies. The presence of HLA-C1 and HLA-C2 groups were typed by PCR. Results: The result showed that the percentage of MAIT cells was slightly higher in the control subjects than in in all stages of CRC patients. Stratification analysis of 46 CRC patients indicated that the percentages of circulating MAIT cells were lower in stage II and higher in stages III, and IV. A higher frequency of HLA - C2 among patients with CRC (87%) compared with control subjects (74.4%) was detected. The protein ex...
Physical inactivity has increased in prevalence among adults in industrialized and developing cou... more Physical inactivity has increased in prevalence among adults in industrialized and developing countries owing to the fact that the majority of job situations require individuals to remain seated for extended periods of time. This research aims to evaluate the influence of cycling on a stationary bike while executing a keyboard assembly task on the task completion time, error percentage, and physiological and subjective measurements. The physiological measures were electroencephalography (EEG) and electrocardiographic (ECG) signal responses, whereas the subjective measures were subjective workload ratings and subjective body discomforts. Two variables were evaluated, namely assembly methods (with versus without pedal exercises at a moderate intensity) and session testing (pre- versus post-test). Thus, the repeated measures design (i.e., assembly method by session testing of participants) was used. According to the completion time, error %, participant self-reports, and ECG and EEG st...
Figure 2. Novomitchellia bursaelongata Harrath, Sluys & Riutort, sp. nov. Holotype. A, photograph... more Figure 2. Novomitchellia bursaelongata Harrath, Sluys & Riutort, sp. nov. Holotype. A, photograph of preserved specimen in dorsal view; some protuberances are seen on the head (arrowheads); arrow indicates the tail (T). B, photograph of preserved specimen in ventral view; arrow indicates the pharynx (PH). Scale bars not available.
Figure 1. Bayesian tree inferred from the 18S rDNA sequences showing the relationship of the new ... more Figure 1. Bayesian tree inferred from the 18S rDNA sequences showing the relationship of the new Novomitchellia species to other Tricladida species included in this analysis. Maximum likelihood (ML) yielded the same topology. Asterisks at nodes indicate posterior probabilities = 1/bootstrap values> 75% obtained respectively in the ML and Bayesian inference analyses. Scale bar: number of substitutions per nucleotide position.
New benzimidazolium salts were synthesized as <i>N</i>-heterocyclic carbene precursor... more New benzimidazolium salts were synthesized as <i>N</i>-heterocyclic carbene precursors. These NHC precursors were metallated with Cu<sub>2</sub>O and CuI in acetone and water under reflux to give novel copper(I) complexes. The structures of these benzimidazolium salts and copper(I) complexes were characterized on the basis of elemental analysis, <sup>1</sup>H NMR, <sup>13</sup>C NMR, IR and LC–MS spectroscopic techniques. The (NHC)Cu(I) complexes <b>3–4</b> were tested against <i>MCF7</i> and <i>MDA-MB-231</i> cancer cells, <i>Escherichia coli</i>, methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and <i>Candida albican</i>s microorganisms, <i>Leishmania major</i> promastigotes and amastigotes, <i>Toxoplasma gondii</i> parasites and against Vero cell line <i>in vitro</i>. The synthesized copper NHC carbene complex <b&g...
Figure 3. Seasonal variation of the prevalence of Ceratomyxa ghannouchensis n. sp. and Ceratomyxa... more Figure 3. Seasonal variation of the prevalence of Ceratomyxa ghannouchensis n. sp. and Ceratomyxa pallida from Boops boops in the Gulf of Gabès.
This dataset contains the digitized treatments in Plazi based on the original journal article Tha... more This dataset contains the digitized treatments in Plazi based on the original journal article Thabet, Aouatef, Abdel-Baki, Abdel-Azeem S., Harrath, Abdel Halim, Mansour, Lamjed (2019): Morphological and molecular aspects of Ceratomyxa ghannouchensis n. sp. and C. pallida Thélohan 1894 infecting the bogueı Boops boops (l.). JOURNAL OF NATURAL HISTORY 53: 541-556, DOI: 10.1080/00222933.2019.1597202
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