British journal of hospital medicine (London, England : 2005), 2006
RefDoc, THE reference in scientific document supply / Refdoc, la référence en fourniture de docum... more RefDoc, THE reference in scientific document supply / Refdoc, la référence en fourniture de documents scientifiques ...
A series of β-ionone derived chalcones were evaluated for cytotoxic activity against various huma... more A series of β-ionone derived chalcones were evaluated for cytotoxic activity against various human cancer cell lines using SRB dye assay. All the compounds displayed moderate to high cytotoxic effect against almost all the cancer cell lines. The results also revealed the effect of substituents of the aromatic ring on their inhibitory potential. In general, compounds bearing electron withdrawing groups such as nitro, fluoro, chloro and bromo showed more inhibitory potential than those bearing electron donating groups. The nitro substituted compound (7h) showed comparatively more inhibitory potential than other derivatives, therefore, it was further investigated for observing its effect on cell morphology in Chinese hamster ovary (CHO) cells by using phase contrast imaging, cell cycle analysis and annexin-FITC apoptosis assay. The treated cells exhibited the characteristics of apoptosis i.e. cell shrinkage, chromatin condensation and nuclear fragmentation, and induced the inhibition of cell proliferation by arresting the cells at G0 phase.
A series of novel 3-salicyloylpyridines (4a-h) were synthesized with good yield by modified Knoev... more A series of novel 3-salicyloylpyridines (4a-h) were synthesized with good yield by modified Knoevenagel-Stobbel method; o-allylation with allyl bromide lead to formation of compounds (5a-h). The synthesized compounds were characterized by spectroscopic techniques and evaluated for cytotoxic activity against human cancer cell lines. Compounds bearing hydroxyl group displayed high cytotoxicity (4a-h) as compared to o-allylated molecules (5a-h). The most active compound 4b was selected for further investigation to look for mechanism of cell death in prostate cancer (PC-3) cells. The apoptotic bodies induced by 4b in PC-3 cells were scanned by confocal microscopy and confirmed by scanning electron microscopy (SEM). Further results obtained from spectrofluorimetric determination of mitochondrial membrane potential (ΔΨm) and intracellular reactive oxygen species (ROS) in treated PC-3 cells revealed that mitochondria dependent apoptosis was involved in the cell death.
... Correspondence to Dr Girish Dwivedi, NIHR Clinical Lecturer in Cardiology, University Departm... more ... Correspondence to Dr Girish Dwivedi, NIHR Clinical Lecturer in Cardiology, University Department of Medicine, City Hospital, Birmingham, B18 7QH, UK. E-mail g.dwivedi{at}bham.ac.uk. A 27-year-old man was being investigated ...
Medical conferences are organised by professional bodies with a view to providing an opportunity ... more Medical conferences are organised by professional bodies with a view to providing an opportunity of continuing medical education and improving the practices of medical professionals. Of late these events have degenerated into entertainment extravaganzas and no longer remain pure academic exercises. This article discusses the possible reasons behind the current state of decay and mechanisms to improve the state of affairs.
... Department of Medicine, University College of Medical Sciences and GTB Hospital, Delhi, India... more ... Department of Medicine, University College of Medical Sciences and GTB Hospital, Delhi, India. Address for correspondence: Dr. Vishal Sharma, E-mail: docvishalsharma/at/gmail.com. ... Sir,. We read with interest the article on rhabdomyolysis secondary to staphylococcal ...
British journal of hospital medicine (London, England : 2005), 2006
RefDoc, THE reference in scientific document supply / Refdoc, la référence en fourniture de docum... more RefDoc, THE reference in scientific document supply / Refdoc, la référence en fourniture de documents scientifiques ...
A series of β-ionone derived chalcones were evaluated for cytotoxic activity against various huma... more A series of β-ionone derived chalcones were evaluated for cytotoxic activity against various human cancer cell lines using SRB dye assay. All the compounds displayed moderate to high cytotoxic effect against almost all the cancer cell lines. The results also revealed the effect of substituents of the aromatic ring on their inhibitory potential. In general, compounds bearing electron withdrawing groups such as nitro, fluoro, chloro and bromo showed more inhibitory potential than those bearing electron donating groups. The nitro substituted compound (7h) showed comparatively more inhibitory potential than other derivatives, therefore, it was further investigated for observing its effect on cell morphology in Chinese hamster ovary (CHO) cells by using phase contrast imaging, cell cycle analysis and annexin-FITC apoptosis assay. The treated cells exhibited the characteristics of apoptosis i.e. cell shrinkage, chromatin condensation and nuclear fragmentation, and induced the inhibition of cell proliferation by arresting the cells at G0 phase.
A series of novel 3-salicyloylpyridines (4a-h) were synthesized with good yield by modified Knoev... more A series of novel 3-salicyloylpyridines (4a-h) were synthesized with good yield by modified Knoevenagel-Stobbel method; o-allylation with allyl bromide lead to formation of compounds (5a-h). The synthesized compounds were characterized by spectroscopic techniques and evaluated for cytotoxic activity against human cancer cell lines. Compounds bearing hydroxyl group displayed high cytotoxicity (4a-h) as compared to o-allylated molecules (5a-h). The most active compound 4b was selected for further investigation to look for mechanism of cell death in prostate cancer (PC-3) cells. The apoptotic bodies induced by 4b in PC-3 cells were scanned by confocal microscopy and confirmed by scanning electron microscopy (SEM). Further results obtained from spectrofluorimetric determination of mitochondrial membrane potential (ΔΨm) and intracellular reactive oxygen species (ROS) in treated PC-3 cells revealed that mitochondria dependent apoptosis was involved in the cell death.
... Correspondence to Dr Girish Dwivedi, NIHR Clinical Lecturer in Cardiology, University Departm... more ... Correspondence to Dr Girish Dwivedi, NIHR Clinical Lecturer in Cardiology, University Department of Medicine, City Hospital, Birmingham, B18 7QH, UK. E-mail g.dwivedi{at}bham.ac.uk. A 27-year-old man was being investigated ...
Medical conferences are organised by professional bodies with a view to providing an opportunity ... more Medical conferences are organised by professional bodies with a view to providing an opportunity of continuing medical education and improving the practices of medical professionals. Of late these events have degenerated into entertainment extravaganzas and no longer remain pure academic exercises. This article discusses the possible reasons behind the current state of decay and mechanisms to improve the state of affairs.
... Department of Medicine, University College of Medical Sciences and GTB Hospital, Delhi, India... more ... Department of Medicine, University College of Medical Sciences and GTB Hospital, Delhi, India. Address for correspondence: Dr. Vishal Sharma, E-mail: docvishalsharma/at/gmail.com. ... Sir,. We read with interest the article on rhabdomyolysis secondary to staphylococcal ...
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