Papers by mutalib Aderogba
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There is ample scientific and empirical evidence supporti the control of neurodegenerative disord... more There is ample scientific and empirical evidence supporti the control of neurodegenerative disorders. Antioxidants may have neuroregenerative roles, by reducing or reversing cellular damage a loss. Although demand for phytotherapeutic agents is growing, there plant–derived extracts gain wider acceptance and use. We have eva africanum (weeping wattle), a plant widespread in the tropics and t acute and chronic pain, anxiety and depression. The dried leaves, b with acetone. Thin layer chromatograms were sprayed with 0.2% methanol for screening for antioxidants. Quantification of antioxida 2, 5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) and L-asco two free radicals, 2,2 ́-azinobis (3-ethyl-benzothiazoline-6-sulfon Results of our study show that the bark and root extracts had high and Trolox, a synthetic vitamin-E analogue. The respective TEAC values for the bark and root extracts, and Trolox were 1.08, 1.28 an μg/mL) was more active than the leaf 6.54 (μg/mL), but much less (3.8...
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Bauhinia monandra (Kurz), traditionally use in established significant anti-diabetic activity was... more Bauhinia monandra (Kurz), traditionally use in established significant anti-diabetic activity was in constituents since the activity demonstrated can be linke compounds. Bioassay directed fractionation of the ethyl led to isolation of two active compounds identified as: Q Quercetin (2). The molecular structures elucidations of b using spectroscopic studies (H NMR, C NMR and MS from this species for the first time. A DPPH spectro evaluate the antioxidant potential of the compounds. Com activity while Compound 1 had lower activity than L-a standard.
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Ife Journal of Science, 2018
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Journal of Ethnopharmacology, 2018
BACKGROUND Anthocleista vogelii (Planch) is used in folk medicine in conditions related to inflam... more BACKGROUND Anthocleista vogelii (Planch) is used in folk medicine in conditions related to inflammation and oxidative stress. This suggests that some of its constituents could possess ability to reduce oxidative damage to cells and ameliorate inflammatory conditions which are two prominent features in the brain of a person suffering from Alzheimer's disease. AIM OF STUDY The leaf extracts were investigated for their bioactive constituents in our quest for novel compounds for the management of Alzheimer's disease. MATERIALS AND METHODS Anthocleista vogelii crude leaf extract obtained from 80% methanol was successively partitioned with n-hexane, dichloromethane (DCM), ethyl acetate (EtOAc) and n-butanol. This gave four solvent fractions of the crude extract. These fractions and isolated compound were subjected to acetylcholinesterase (AChE) inhibitory activity test using the microplate assay method. The structure of the isolated compound was determined using spectroscopic methods (1D and 2D NMR, and MS). RESULTS The results of the acetylcholinesterase inhibitory assay revealed that n-BuOH and ethyl acetate fractions had the best activity with IC50 of 564.58 and 727.63 µg/mL respectively. Activity directed fractionation of each of these fractions led to the isolation of the same compound identified as a C-glucosylflavone; isovitexin-7-O-methyl ether (swertisin). This rare C-glucosylflavone co-exist as two rotamers and exhibited duplication of NMR signals. It is reported from this plant for the first time. Swertisin displayed better acetylcholinesterase inhibitory activity with IC50 of 32.09 µg/mL than physostigmine (eserine) with IC50 of 56.09 µg/mL used as a standard. CONCLUSIONS This study reports isolation and characterization of a rare C-glycosylflavone; isovitexin-7-O-methyl ether (swertisin). Isolated compound exhibited duplication of NMR signals and better acetylcholinesterase inhibitory activity than eserine used as a standard. The activity exhibited by this compound suggests that it could be useful in the management of Alzheimer's disease.
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South African Journal of Botany, 2017
Abstract Pappea capensis is a medicinal plant widely used in the management of eye infections, se... more Abstract Pappea capensis is a medicinal plant widely used in the management of eye infections, sexually transmitted infections and as an aphrodisiac in South Africa. Two flavonoids quercetin-3-O-rhamnoside ( 1 ) and epicatechin ( 2 ) were isolated through bio-assay guided fractionation. The extracts and isolated compounds were evaluated for antimicrobial, antigonococcal, antioxidant and cytotoxicity potential. The ethyl acetate fraction exhibited a broad spectrum of activity against Bacillus subtilis , Staphylococcus aureus , Escherichia coli and Candida albicans with MIC values ranging from 0.39 to 0.78 mg/mL. In the DPPH radical scavenging assay, extracts and isolated compounds showed free radical scavenging activity with EC 50 values ranging from 6.34 to 158.00 μg/mL. The isolated compound epicatechin ( 2 ) exhibited higher antioxidant activity in the β -carotene-linoleic acid model system (EC 50 = 9.64 μg/mL) than the ascorbic acid standard (EC 50 = 13.37 μg/mL). Cytotoxicity of the samples was determined using the MTT (3-(4,5-dimethylthiazol)-2,5-diphenyl tetrazolium bromide) assay against Vero monkey kidney cells. Little to no cytotoxicity of the extracts and isolated compounds was observed. These results may help to discover new chemical classes of natural antimicrobial and antioxidant substances that could serve as selective agents for infectious microbial diseases.
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British Journal of Pharmaceutical Research, 2015
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Journal of Food Processing and Preservation, 2015
The total phenol and antioxidant activity of the crude extract, dichloromethane, ethylacetate and... more The total phenol and antioxidant activity of the crude extract, dichloromethane, ethylacetate and butanol fractions of the leaves of Bauhinia species and Piliostigma reticulatum plants were determined and EC50 value calculated. The extract with highest antioxidant activity was used in the stabilization study of freshly extracted groundnut oil samples. The results revealed that ethylacetate fraction of P. reticulatum had the highest total phenolic content (106.50 ± 2.26 mg gallic acid equivalent per gram extract) and the lowest EC50 value (8.29 μg/mL). The stabilization study revealed that the plant extract-protected groundnut oil recorded the least values for lipid hydroperoxide, thiobarbituric acid reactive substances and p-anisidine value and the least reduction in total tocopherol. This showed that the plant extract had sparring role on tocopherol. It can be concluded that ethylacetate fraction of P. reticulatum was a better antioxidant than butylated hydroxyanisole and could be used as an antioxidant in vegetable oil. Practical Applications The etiology of some diseases such as cancer has been linked to synthetic antioxidant used in the preservation of vegetable oil. Hence, the replacement of these synthetic compounds with extracts obtained from medicinal plants such as Bauhinia species and P. reticulatum will be a step in the right direction. The ethylacetate extract from P. reticulatum showed a great potential as an antioxidant against lipid oxidation. The extract of this plant can be used as a possible replacement for synthetic antioxidants for the preservation of the nutritional quality of vegetable oil.
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South African Journal of Botany, 2014
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South African Journal of Botany, 2014
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South African Journal of Botany, 2012
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Journal of Pharmacy and Pharmacology
Objectives The effects of a root extract of Zanthoxylum zanthoxyloides on neuroinflammation in BV... more Objectives The effects of a root extract of Zanthoxylum zanthoxyloides on neuroinflammation in BV-2 microglia stimulated with LPS and hemozoin were investigated. Methods ELISA, enzyme immunoassay and Griess assay were used to evaluate levels of cytokines, PGE2 and NO in culture supernatants, respectively. Microglia-mediated neurotoxicity was evaluated using a BV-2 microglia-HT-22 neuron transwell co-culture. Key findings Treatment with Z. zanthoxyloides caused reduced elevated levels of TNFα, IL-6, IL-1β, NO and PGE2, while increasing the levels of IL-10. In addition, there were reduced levels of iNOS and COX-2 proteins. This was accompanied by a prevention of microglia-mediated damage to HT-22 mouse hippocampal neurons. Z. zanthoxyloides reduced elevated levels of phospho-IκB and phospho-p65, while preventing degradation of IκB protein and DNA binding of p65. Further mechanistic studies revealed that Z. zanthoxyloides reduced the levels of pro-IL-1β and IL-1β in hemozoin-activated ...
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Natural Product Communications
Croton sylvaticus is a medicinal plant widely employed in the management of inflammatory conditio... more Croton sylvaticus is a medicinal plant widely employed in the management of inflammatory conditions, infections and oxidative stress related diseases such as insomnia, abdominal and internal inflammations. The activities demonstrated by the plant extracts support that its constituents possess the ability to reduce oxidative damage to body cells. Repeated column fractionation of the ethyl acetate fraction of a 20% aqueous methanol leaf extract using acetylcholinesterase (AChE) activity directed on Sephadex LH-20 afforded three compounds identified by mass spectrometry, and 1D and 2D NMR spectroscopy as: 2′-(3″,4″-dihydroxyphenyl)-ethyl-4-hydroxybenzoate (1), 3, 3′, 4′, 5, 7-pentahydroxyflavone (quercetin) (2), and 3, 4′, 5, 7-tetrahydroxyflavone (kaempferol) (3). These compounds are reported from the leaf extracts of C. sylvaticus for the first time and compound 1 is a new natural product. The microplate technique was used to determine the inhibitory effects of the plant extracts (cr...
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Phytotherapy Research
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Light induced oxidation of the oils of five non-conventional oil sources namely: I. gabonensis (W... more Light induced oxidation of the oils of five non-conventional oil sources namely: I. gabonensis (Wild Mango or Dica nut) Seed, D. edulis (African native pear) seed, D. edulis flesh, P. americana (Avocado pear) flesh and T. catappa (Almond) seed were monitored under light of different intensities (Dark, fluorescent light, daylight and direct sunlight). G. Max (Soya bean) oil was used as reference sample for this comparative study. I. gabonensis has the highest stability under the tested conditions. Peroxidation process was accelerated in all the tested oils under direct sunlight. G. max oil recorded the highest peroxide value. All the tested oil samples except I. gabonesis exhibited higher percentage antioxidant activity than the conventional oil from G. max . The strongest effect was obtained for D. edulis flesh oil.
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Journal of ethnopharmacology, Jan 25, 2013
Searsia chirindensis is used in South African traditional medicine for management of bacterial in... more Searsia chirindensis is used in South African traditional medicine for management of bacterial infections such as diarrhoea. Aim of the study was to examine the phytochemical composition from the leaves of Searsia chirindensis that is responsible for the ethnomedicinal use of this plant. The crude extract (80% methanol) was extracted sequentially with dichloromethane (DCM), ethyl acetate (EtOAc) and n-butanol. The extracts and isolated compounds were tested for their antibacterial activity against Gram-negative (Campylobacter jejuni, Escherichia coli and Shigella flexneri) and Gram-positive (Staphylococcus aureus) bacterial strains using the microdilution method. Bioguided fractionation of EtOAc fraction afforded five phenolic compounds. Structural elucidation was carried out using NMR (1D and 2D) spectroscopic analyses. Of the three fractions obtained from the crude extract, EtOAc was the most active and its fractionation afforded methyl gallate (1), and four flavonol glycosides: m...
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Nigerian Journal of Natural Products and Medicine, 2014
ABSTRACT Ceiba pentandra widely employed in the management of inflammatory conditions, infections... more ABSTRACT Ceiba pentandra widely employed in the management of inflammatory conditions, infections and oxidative stress related diseases was investigated for its active constituents. A DPPH-directed fractionation of the ethyl acetate solvent fraction of a 20% aqueous methanol leaves extract of Ceiba pentandra afforded a clovamide derivative, N-trans-caffeoyl-DOPA-methyl ester (1) together with a flavonoid, acacetin-7-O-rutinoside, Linarin (2) and 3,4- dihydroxybenzoic acid, protocatechuic acid (3). Structure elucidation of the isolated compounds was carried out using spectroscopic techniques: mass spectrometry (ESI-TOF-MS), 1D NMR (1H, 13C and DEPT) as well as 2D experiments (GCOSY, GHSQC and GHMBC). In the qualitative antioxidant assay, compounds 1- 3 bleached the DPPH purple colour indicating free radical scavenging potential (antioxidant activity). Isolation of these antioxidant compounds could provide rationale for the use of this plant in traditional medicine in the management of the oxidative stress related diseases and infections.
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Nigerian Journal of Natural Products and Medicine, 2012
ABSTRACT Dennettia tripetala a Nigerian medicinal plant widely employed in the management of oxid... more ABSTRACT Dennettia tripetala a Nigerian medicinal plant widely employed in the management of oxidative stress related diseases and infections was investigated for its active constituents. A DPPH (1,1-diphenyl-2-picrylhydrazine) guided fractionation was used to target and isolate the antioxidant constituents of the ethyl acetate solvent fraction of a 20% aqueous methanol leaf extract. Two flavonoid glycosides were isolated and identified by spectral data (1H, and 13C NMR, along with ESI-TOF-MS spectrometry) as: quercetin-3-O-arabinofuranoside, avicularin (1) and vitexin-2'’-O-rhamnoside (2). In the qualitative antioxidant assay, the compounds instantly bleached the DPPH purple colour indicating free radical scavenging potential (antioxidant activity). In the micro-well dilution assay, Compound (1) was the most active with MIC values of 0.081 mg/mL against Staphylococcus aureus NCTC 6571 and 2.50 mg/mL against Escherichia coli NCTC 8196 compared to the standard drug streptomycin which had MICs of 0.039 mg/mL and 0.156 mg/mL respectively against the two organisms. None of the compounds was able to inhibit the growth of the fungi strain used at the tested concentrations. Isolation of these antioxidant and antimicrobial compounds could provide rationale for the ethnomedicinal use of this plant in traditional medicine in the management of the oxidative stress related diseases and infections.
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