Đai lý vé máy bay Bình Dương là đại lý chi nhánh của tổng đại lý vé máy bay Việt Đức là đại lý ch... more Đai lý vé máy bay Bình Dương là đại lý chi nhánh của tổng đại lý vé máy bay Việt Đức là đại lý chính thức của các hãng hàng không xin chia sẻ một vài thông tin để quý khách hàng nếu muốn Tết này về Hà Nội bằng vé máy bay giá rẻ thì tham khảo nha.
As the regulator of pituitary reproductive hormone synthesis, the hypothalamic neuropeptide GnRH ... more As the regulator of pituitary reproductive hormone synthesis, the hypothalamic neuropeptide GnRH is the central regulator of reproduction. A hallmark of GnRH action is the differential control of gene expression in pituitary gonadotropes through varied pulsatile stimulation. Among other signaling events, GnRH activation of the ERK family of MAPKs plays a significant role in the transcriptional regulation of the luteinizing hormone β-subunit gene and regulation of cap-dependent translation. We evaluated the ERK response to different GnRH pulse amplitudes in the gonadotrope cell line LβT2. We found that low-amplitude stimulation with GnRH invokes a rapid and transient ERK activation, whereas high-amplitude stimulation invokes a prolonged activation specifically in the cytoplasm fraction of LβT2 cells. Nuclear and cytoplasmic targets of ERK, Ets-like gene 1, and eukaryotic initiation factor 4E, respectively, are similarly activated. Feedback control of ERK activation occurs mainly through the dual-specificity protein phosphatases (DUSPs). DUSP1 is localized to the nucleus in LβT2 cells but DUSP4, another member implicated in GnRH feedback, exists in both the nucleus and cytoplasm. Manipulation of nuclear DUSP activity through overexpression or knockdown of Dusp1 modulates the ERK response to low and high GnRH pulse amplitudes and activation of the Lhb promoter. Dusp1 overexpression abolishes sustained ERK activation and inhibits Lhb promoter activity induced by high amplitude pulses. Conversely, Dusp1 knockdown enhances ERK activation by low-amplitude stimulation and increases stimulation of Lhb promoter activity. We conclude that DUSP1 feedback activity modulates ERK activation and the transcriptional response to GnRH.
Irmmw Thz2007 Conference Digest of the Joint 32nd International Conference on Infrared and Millimetre Waves and 15th International Conference on Terahertz Electronics 660 661, 2007
The conversion of polycrystalline pharmaceutical materials into their cocrystals has been monitor... more The conversion of polycrystalline pharmaceutical materials into their cocrystals has been monitored using terahertz (THz) spectroscopy. Eight cocrystal systems have been studied and their THz spectra were found to exhibit differences from the constituent materials. Cocrystals of theophylline and DL and L forms of malic acid were clearly differentiated using THz spectroscopy while powder X-ray diffraction (PXRD) results were less conclusive.
Infrared and Millimeter Waves, Conference Digest of the 2004 Joint 29th International Conference on 2004 and 12th International Conference on Terahertz Electronics, 2004., 2004
... [5] EDL Smith et al., 'The determination of the crystal sl~~uch~re of anhydrous ... more ... [5] EDL Smith et al., 'The determination of the crystal sl~~uch~re of anhydrous theophylline by X-ray powder difFraction with a systematic search algorithm, lattice enerm calculations, and C and 'IN solid-state NMR A question of polymorphism in a given unit cell, J. Phys. Chem. ...
A rapid, simple, and reliable method using ultra-performance LC/MS/MS (UPLC/MS/MS) was developed ... more A rapid, simple, and reliable method using ultra-performance LC/MS/MS (UPLC/MS/MS) was developed for determination of ochratoxin A (OTA) in processed cereal products. OTA was ultrasonically extracted from the sample with acetonitrile-water (80 + 20, v/v), and the extract was then injected into the UPLC/MS/MS system after filtration. The calibration curves had good linearity with coefficients of determination greater than 0.999. Recoveries of OTA were in the range of 90-104%. LOD and LOQ of OTA in samples were 0.6 and 2.0 ng/g, respectively, and no significant matrix effect was found. This method was applied to determine OTA in 25 oat-based cereal samples. OTA was detected in five samples (20%) in the range of 2.4 to 7.3 ng/g.
A new, simple, and sensitive method was developed for extraction of ochratoxin A (OTA) in beer co... more A new, simple, and sensitive method was developed for extraction of ochratoxin A (OTA) in beer combined with HPLC-fluorescence detector. Samples were extracted by stir bar sorptive extraction (SBSE) followed by liquid desorption using commercially available Twister EG-Silicone. The main parameters influencing SBSE, including phase ratio, extraction time, stirring speed, ionic strength, organic modifier, pH, temperature, desorption mode, desorption solvent, and desorption time were optimized. Under the optimal conditions, assay was performed on 4 mL of samples acidified at pH 1.5. The samples were extracted for 180 min at a stirring speed of 800 rpm followed by desorption of analyte using 1 mL methanol under sonication for 45 min. The extract was evaporated at 50 degrees C under a gentle nitrogen stream, then redissolved in 200 microL of methanol-water (50 + 50, v/v). After each use, the stir bar was cleaned by sonication in methanol for 30 min three times. The method provided good linearity of the calibration curve with coefficients of determination greater than 0.999. Recoveries of OTA were greater than 83% with RSD lower than 10%, and LOD of OTA in beer was 0.64 ng/L. This method was applied to determine OTA in 19 beer samples. OTA was detected in 12 samples (63%) in the range of 0.01-0.27 ng/mL.
A target-unrelated peptide (TUP) can arise in phage display selection experiments as a result of ... more A target-unrelated peptide (TUP) can arise in phage display selection experiments as a result of a propagation advantage exhibited by the phage clone displaying the peptide. We previously characterized HAIYPRH, from the M13-based Ph.D.-7 phage display library, as a propagation-related TUP resulting from a G→A mutation in the Shine-Dalgarno sequence of gene II. This mutant was shown to propagate in Escherichia coli at a dramatically faster rate than phage bearing the wild-type Shine-Dalgarno sequence. We now report 27 additional fast-propagating clones displaying 24 different peptides and carrying 14 unique mutations. Most of these mutations are found either in or upstream of the gene II Shine-Dalgarno sequence, but still within the mRNA transcript of gene II. All 27 clones propagate at significantly higher rates than normal library phage, most within experimental error of wild-type M13 propagation, suggesting that mutations arise to compensate for the reduced virulence caused by the insertion of a lacZα cassette proximal to the replication origin of the phage used to construct the library. We also describe an efficient and convenient assay to diagnose propagation-related TUPS among peptide sequences selected by phage display.
New strategies for prevention and treatment of type 2 diabetes (T2D) require improved insight int... more New strategies for prevention and treatment of type 2 diabetes (T2D) require improved insight into disease etiology. We analyzed 386,731 common single-nucleotide polymorphisms (SNPs) in 1464 patients with T2D and 1467 matched controls, each characterized for measures of glucose metabolism, lipids, obesity, and blood pressure. With collaborators (FUSION and WTCCC/UKT2D), we identified and confirmed three loci associated with T2D-in a noncoding region near CDKN2A and CDKN2B, in an intron of IGF2BP2, and an intron of CDKAL1-and replicated associations near HHEX and in SLC30A8 found by a recent whole-genome association study. We identified and confirmed association of a SNP in an intron of glucokinase regulatory protein (GCKR) with serum triglycerides. The discovery of associated variants in unsuspected genes and outside coding regions illustrates the ability of genome-wide association studies to provide potentially important clues to the pathogenesis of common diseases.
Đai lý vé máy bay Bình Dương là đại lý chi nhánh của tổng đại lý vé máy bay Việt Đức là đại lý ch... more Đai lý vé máy bay Bình Dương là đại lý chi nhánh của tổng đại lý vé máy bay Việt Đức là đại lý chính thức của các hãng hàng không xin chia sẻ một vài thông tin để quý khách hàng nếu muốn Tết này về Hà Nội bằng vé máy bay giá rẻ thì tham khảo nha.
As the regulator of pituitary reproductive hormone synthesis, the hypothalamic neuropeptide GnRH ... more As the regulator of pituitary reproductive hormone synthesis, the hypothalamic neuropeptide GnRH is the central regulator of reproduction. A hallmark of GnRH action is the differential control of gene expression in pituitary gonadotropes through varied pulsatile stimulation. Among other signaling events, GnRH activation of the ERK family of MAPKs plays a significant role in the transcriptional regulation of the luteinizing hormone β-subunit gene and regulation of cap-dependent translation. We evaluated the ERK response to different GnRH pulse amplitudes in the gonadotrope cell line LβT2. We found that low-amplitude stimulation with GnRH invokes a rapid and transient ERK activation, whereas high-amplitude stimulation invokes a prolonged activation specifically in the cytoplasm fraction of LβT2 cells. Nuclear and cytoplasmic targets of ERK, Ets-like gene 1, and eukaryotic initiation factor 4E, respectively, are similarly activated. Feedback control of ERK activation occurs mainly through the dual-specificity protein phosphatases (DUSPs). DUSP1 is localized to the nucleus in LβT2 cells but DUSP4, another member implicated in GnRH feedback, exists in both the nucleus and cytoplasm. Manipulation of nuclear DUSP activity through overexpression or knockdown of Dusp1 modulates the ERK response to low and high GnRH pulse amplitudes and activation of the Lhb promoter. Dusp1 overexpression abolishes sustained ERK activation and inhibits Lhb promoter activity induced by high amplitude pulses. Conversely, Dusp1 knockdown enhances ERK activation by low-amplitude stimulation and increases stimulation of Lhb promoter activity. We conclude that DUSP1 feedback activity modulates ERK activation and the transcriptional response to GnRH.
Irmmw Thz2007 Conference Digest of the Joint 32nd International Conference on Infrared and Millimetre Waves and 15th International Conference on Terahertz Electronics 660 661, 2007
The conversion of polycrystalline pharmaceutical materials into their cocrystals has been monitor... more The conversion of polycrystalline pharmaceutical materials into their cocrystals has been monitored using terahertz (THz) spectroscopy. Eight cocrystal systems have been studied and their THz spectra were found to exhibit differences from the constituent materials. Cocrystals of theophylline and DL and L forms of malic acid were clearly differentiated using THz spectroscopy while powder X-ray diffraction (PXRD) results were less conclusive.
Infrared and Millimeter Waves, Conference Digest of the 2004 Joint 29th International Conference on 2004 and 12th International Conference on Terahertz Electronics, 2004., 2004
... [5] EDL Smith et al., 'The determination of the crystal sl~~uch~re of anhydrous ... more ... [5] EDL Smith et al., 'The determination of the crystal sl~~uch~re of anhydrous theophylline by X-ray powder difFraction with a systematic search algorithm, lattice enerm calculations, and C and 'IN solid-state NMR A question of polymorphism in a given unit cell, J. Phys. Chem. ...
A rapid, simple, and reliable method using ultra-performance LC/MS/MS (UPLC/MS/MS) was developed ... more A rapid, simple, and reliable method using ultra-performance LC/MS/MS (UPLC/MS/MS) was developed for determination of ochratoxin A (OTA) in processed cereal products. OTA was ultrasonically extracted from the sample with acetonitrile-water (80 + 20, v/v), and the extract was then injected into the UPLC/MS/MS system after filtration. The calibration curves had good linearity with coefficients of determination greater than 0.999. Recoveries of OTA were in the range of 90-104%. LOD and LOQ of OTA in samples were 0.6 and 2.0 ng/g, respectively, and no significant matrix effect was found. This method was applied to determine OTA in 25 oat-based cereal samples. OTA was detected in five samples (20%) in the range of 2.4 to 7.3 ng/g.
A new, simple, and sensitive method was developed for extraction of ochratoxin A (OTA) in beer co... more A new, simple, and sensitive method was developed for extraction of ochratoxin A (OTA) in beer combined with HPLC-fluorescence detector. Samples were extracted by stir bar sorptive extraction (SBSE) followed by liquid desorption using commercially available Twister EG-Silicone. The main parameters influencing SBSE, including phase ratio, extraction time, stirring speed, ionic strength, organic modifier, pH, temperature, desorption mode, desorption solvent, and desorption time were optimized. Under the optimal conditions, assay was performed on 4 mL of samples acidified at pH 1.5. The samples were extracted for 180 min at a stirring speed of 800 rpm followed by desorption of analyte using 1 mL methanol under sonication for 45 min. The extract was evaporated at 50 degrees C under a gentle nitrogen stream, then redissolved in 200 microL of methanol-water (50 + 50, v/v). After each use, the stir bar was cleaned by sonication in methanol for 30 min three times. The method provided good linearity of the calibration curve with coefficients of determination greater than 0.999. Recoveries of OTA were greater than 83% with RSD lower than 10%, and LOD of OTA in beer was 0.64 ng/L. This method was applied to determine OTA in 19 beer samples. OTA was detected in 12 samples (63%) in the range of 0.01-0.27 ng/mL.
A target-unrelated peptide (TUP) can arise in phage display selection experiments as a result of ... more A target-unrelated peptide (TUP) can arise in phage display selection experiments as a result of a propagation advantage exhibited by the phage clone displaying the peptide. We previously characterized HAIYPRH, from the M13-based Ph.D.-7 phage display library, as a propagation-related TUP resulting from a G→A mutation in the Shine-Dalgarno sequence of gene II. This mutant was shown to propagate in Escherichia coli at a dramatically faster rate than phage bearing the wild-type Shine-Dalgarno sequence. We now report 27 additional fast-propagating clones displaying 24 different peptides and carrying 14 unique mutations. Most of these mutations are found either in or upstream of the gene II Shine-Dalgarno sequence, but still within the mRNA transcript of gene II. All 27 clones propagate at significantly higher rates than normal library phage, most within experimental error of wild-type M13 propagation, suggesting that mutations arise to compensate for the reduced virulence caused by the insertion of a lacZα cassette proximal to the replication origin of the phage used to construct the library. We also describe an efficient and convenient assay to diagnose propagation-related TUPS among peptide sequences selected by phage display.
New strategies for prevention and treatment of type 2 diabetes (T2D) require improved insight int... more New strategies for prevention and treatment of type 2 diabetes (T2D) require improved insight into disease etiology. We analyzed 386,731 common single-nucleotide polymorphisms (SNPs) in 1464 patients with T2D and 1467 matched controls, each characterized for measures of glucose metabolism, lipids, obesity, and blood pressure. With collaborators (FUSION and WTCCC/UKT2D), we identified and confirmed three loci associated with T2D-in a noncoding region near CDKN2A and CDKN2B, in an intron of IGF2BP2, and an intron of CDKAL1-and replicated associations near HHEX and in SLC30A8 found by a recent whole-genome association study. We identified and confirmed association of a SNP in an intron of glucokinase regulatory protein (GCKR) with serum triglycerides. The discovery of associated variants in unsuspected genes and outside coding regions illustrates the ability of genome-wide association studies to provide potentially important clues to the pathogenesis of common diseases.
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