Background and aim : Diabetes is a major cause of chronic kidney disease (CKD). Urine albumin and... more Background and aim : Diabetes is a major cause of chronic kidney disease (CKD). Urine albumin and estimated glomerular filtration rate (eGFR) are the two key markers for chronic kidney disease (CKD). The aim of our work was to explore the possibility of plasma cystatin C and urinary sE.cadherin as useful biomarkers for early detection of diabetic nephropathy (DN). Methods: A total number of 80 subjects were included and were classified into three main groups. Group I (20) normal subjects with no history of DM, hypertension or other diseases and with albumin / creatinine (Alb / Cr) ratio below 30 mg/ g. Group II (30) type 2 diabetic patients with Alb / Cr ratio below 30 mg/ g. Group III (30) type 2 diabetic patients with Alb / Cr ratio between 30 and 300 mg/ g. The latter two groups were divided in two sub groups A and B according to GFR by MDRD: normal (≥ 90 ml /min / 1.73 m2) and < 90 ml /min / 1.73 m2. All subjects underwent urine analysis, complete blood picture, liver functio...
Background: Systemic lupus erythematosus (SLE) is a chronic, multisystem, autoimmune disorder cha... more Background: Systemic lupus erythematosus (SLE) is a chronic, multisystem, autoimmune disorder characterised by widespread inflammation of connective tissues affecting the skin, joints, kidneys, heart, lungs, and nervous system. SLE is associated with significant morbidity, thus requiring prompt evaluation, appropriate therapy and long-term follow-up. Objective: This study was performed to measure serum adiponectin in SLE patients and to correlate it with SLEDAI and to evaluate its role as a biomarker of activity in SLE patients specially renal in the hope of preventing or at least postponing renal damage. Patients and methods: This study included 72 subjects, 36 patients and 36 volunteers. The patients were divided into three groups. Group I (active renal) 12 patients having renal disease activity, group II (active non-renal) 12 patients having non-renal disease activity and group III (inactive SLE). 36 apparently healthy volunteers were included (group IV) as a control group. Resul...
1.ABSTRACTIntroductionWorldwide, cancer is a significant public health problem. Curcumin exhibits... more 1.ABSTRACTIntroductionWorldwide, cancer is a significant public health problem. Curcumin exhibits anti-inflammatory, antiproliferative, and anticancer properties when used in medicine. Investigated study for Curcumin’s chemopreventive mechanism against human malignancies, this research examined the cellular and molecular alterations generated by curcumin modified compound in breast cancer (MCF-7) cell lines. Oncogenic EGFR and VEGFR2 mutations lead to the formation, invasion, and maintenance of malignant phenotypes in humans, including breast cancer. Studied prognostic markers such as C-myc and Ki67 in breast cancer, and the apoptotic gene as Caspase-3 have been done.Aim of the workThe purpose of this study is to determine the therapeutic efficacy of curcumin nanoparticles and nanocapsules in breast cancer cell lines (MCF7).Materials and methodsWe used real-time PCR to assess the expression of the C-myc, Ki67, EGFR, VEGFR2, and Caspase-3 genes in MCF7 cells treated with Curcumin nan...
Background: Chronic haemodialysis patients are at high risk for infection because the process of ... more Background: Chronic haemodialysis patients are at high risk for infection because the process of haemodialysis (HM) requires vascular access for prolonged periods. The humanCytomegalo Virus (CMV) is a DNA virus belonging to the Herpesviridae family, Betaherpsvirnae subfamily and Cytomegalo virus gender, known as Human Herpes Virus Type 5, one of the main causes of morbidity. Acute CMV infection is characterized by system-wide viremia after which latency and lifelong persistence is established in selected cells such as CD34+ monocytes and hematopoietic progenitor cells in humans. Objective: The work was designed for early detection of CMV infection and metabolic syndrome (MetS) in patients on HD. Patients and Methods: In this cross-sectional study, 80 patients under regular HD due to end-stage renal disease (ESRD) had participated in the study from HD Unite of Nasr City Health Insurance Hospital in Cairo in the period from June 2019 to December 2019. Results: In the present study, ...
Phytochemical investigation of the mangrove plant Lumnitzera racemosa WILLD (Fam: Combretaceae) h... more Phytochemical investigation of the mangrove plant Lumnitzera racemosa WILLD (Fam: Combretaceae) has resulted in the isolation of 8 compounds by various chromatographic techniques (silica gel, ODS column chromatography and HPLC). The structures of these compounds were determined by spectrometric analysis (UV, IR, HR-ESI-MS, 1D and 2D-NMR). All the isolated compounds were evaluated for their hepatoprotective activity. The Compound number 8 showed high hepatoprotective activity against acetoaminophen and compound 1 showed moderate activity compared to glycyrrhizin as the positive control using human Hep G2 cell line. The Compounds number 2, 3, 4, 5, 6 and 8 showed the highest DPPH radical scavenging activity (IC50: 14.74±5.66, 18.88±3.56, 5.93±2.1, 7.17±0.42, 7.38±1.03 and 15.72±1.18 μM, respectively), while compounds 1 and 7 showed lowest DPPH radical scavenging activity comparable with the standard trolox (IC50: 5.93±2.19 M).
Animals fed with a high amount of a wide range of antioxidants in their diet are significantly pr... more Animals fed with a high amount of a wide range of antioxidants in their diet are significantly protected against oxidative stress. Powerful antioxidant substances such as vitamin E, vitamin C, and carotenoids are present naturally in red-hot pepper (RHP). This study hypothesized that using RHP may provide protection against oxidative stress and enhance animal physiological responses. Thus, this study aimed to investigate the effect of feeding New Zealand white rabbits with RHP-supplemented diets on their physiological and biochemical responses. New Zealand White rabbits (age = 6 weeks, n = 48) were split equally into three groups (n = 16 in each group). One group was fed a basal diet only (control group), with the other two groups fed a basal diet along with 1 and 2% RHP. Mass spectrometric analysis for the RHP methanolic extract showed some phenolic compounds, such as p-coumaric, sinapinic acids, vanillic, and luteolin, as well as catechin and its isomers. Hepatic antioxidant enzym...
Journal of the Egyptian Society of Parasitology, 2016
1,4-Dioxane (DX) with two oxygen atoms make it hydrophilic and infinitely soluble in water. As a ... more 1,4-Dioxane (DX) with two oxygen atoms make it hydrophilic and infinitely soluble in water. As a synthetic organic compound, it used widely throughout industry as a solvent. Dioxane causes numerous human ailments such as liver damage and kidney failure. It has been shown in research to be carcinogenic to animals, and is a potential carcinogen to humans. Daily administration for 1,4-dioxane (100 mg/kg body weight) in drinking water for rats weighing 120 g, except for normal control group. Experimental animal for 42 days was followed through body weight, serum alkaline phosphatase, serum creatinine, malondialdehyde, and catalase enzyme activity; beside histological patterns for liver, kidney, brain and ovary sections. Protection treatment has been offered using oral injection N-acetyl cysteine (100 mg/kg b.wt.), and fresh 200 mg/kg b.wt. in diet meal for each of nabk, husk, and sycamore in separated groups. Body weight and CAT activity have decreased by 25.8, and 68.7%, respectively. ...
Background and aim : Diabetes is a major cause of chronic kidney disease (CKD). Urine albumin and... more Background and aim : Diabetes is a major cause of chronic kidney disease (CKD). Urine albumin and estimated glomerular filtration rate (eGFR) are the two key markers for chronic kidney disease (CKD). The aim of our work was to explore the possibility of plasma cystatin C and urinary sE.cadherin as useful biomarkers for early detection of diabetic nephropathy (DN). Methods: A total number of 80 subjects were included and were classified into three main groups. Group I (20) normal subjects with no history of DM, hypertension or other diseases and with albumin / creatinine (Alb / Cr) ratio below 30 mg/ g. Group II (30) type 2 diabetic patients with Alb / Cr ratio below 30 mg/ g. Group III (30) type 2 diabetic patients with Alb / Cr ratio between 30 and 300 mg/ g. The latter two groups were divided in two sub groups A and B according to GFR by MDRD: normal (≥ 90 ml /min / 1.73 m2) and < 90 ml /min / 1.73 m2. All subjects underwent urine analysis, complete blood picture, liver functio...
Background: Systemic lupus erythematosus (SLE) is a chronic, multisystem, autoimmune disorder cha... more Background: Systemic lupus erythematosus (SLE) is a chronic, multisystem, autoimmune disorder characterised by widespread inflammation of connective tissues affecting the skin, joints, kidneys, heart, lungs, and nervous system. SLE is associated with significant morbidity, thus requiring prompt evaluation, appropriate therapy and long-term follow-up. Objective: This study was performed to measure serum adiponectin in SLE patients and to correlate it with SLEDAI and to evaluate its role as a biomarker of activity in SLE patients specially renal in the hope of preventing or at least postponing renal damage. Patients and methods: This study included 72 subjects, 36 patients and 36 volunteers. The patients were divided into three groups. Group I (active renal) 12 patients having renal disease activity, group II (active non-renal) 12 patients having non-renal disease activity and group III (inactive SLE). 36 apparently healthy volunteers were included (group IV) as a control group. Resul...
1.ABSTRACTIntroductionWorldwide, cancer is a significant public health problem. Curcumin exhibits... more 1.ABSTRACTIntroductionWorldwide, cancer is a significant public health problem. Curcumin exhibits anti-inflammatory, antiproliferative, and anticancer properties when used in medicine. Investigated study for Curcumin’s chemopreventive mechanism against human malignancies, this research examined the cellular and molecular alterations generated by curcumin modified compound in breast cancer (MCF-7) cell lines. Oncogenic EGFR and VEGFR2 mutations lead to the formation, invasion, and maintenance of malignant phenotypes in humans, including breast cancer. Studied prognostic markers such as C-myc and Ki67 in breast cancer, and the apoptotic gene as Caspase-3 have been done.Aim of the workThe purpose of this study is to determine the therapeutic efficacy of curcumin nanoparticles and nanocapsules in breast cancer cell lines (MCF7).Materials and methodsWe used real-time PCR to assess the expression of the C-myc, Ki67, EGFR, VEGFR2, and Caspase-3 genes in MCF7 cells treated with Curcumin nan...
Background: Chronic haemodialysis patients are at high risk for infection because the process of ... more Background: Chronic haemodialysis patients are at high risk for infection because the process of haemodialysis (HM) requires vascular access for prolonged periods. The humanCytomegalo Virus (CMV) is a DNA virus belonging to the Herpesviridae family, Betaherpsvirnae subfamily and Cytomegalo virus gender, known as Human Herpes Virus Type 5, one of the main causes of morbidity. Acute CMV infection is characterized by system-wide viremia after which latency and lifelong persistence is established in selected cells such as CD34+ monocytes and hematopoietic progenitor cells in humans. Objective: The work was designed for early detection of CMV infection and metabolic syndrome (MetS) in patients on HD. Patients and Methods: In this cross-sectional study, 80 patients under regular HD due to end-stage renal disease (ESRD) had participated in the study from HD Unite of Nasr City Health Insurance Hospital in Cairo in the period from June 2019 to December 2019. Results: In the present study, ...
Phytochemical investigation of the mangrove plant Lumnitzera racemosa WILLD (Fam: Combretaceae) h... more Phytochemical investigation of the mangrove plant Lumnitzera racemosa WILLD (Fam: Combretaceae) has resulted in the isolation of 8 compounds by various chromatographic techniques (silica gel, ODS column chromatography and HPLC). The structures of these compounds were determined by spectrometric analysis (UV, IR, HR-ESI-MS, 1D and 2D-NMR). All the isolated compounds were evaluated for their hepatoprotective activity. The Compound number 8 showed high hepatoprotective activity against acetoaminophen and compound 1 showed moderate activity compared to glycyrrhizin as the positive control using human Hep G2 cell line. The Compounds number 2, 3, 4, 5, 6 and 8 showed the highest DPPH radical scavenging activity (IC50: 14.74±5.66, 18.88±3.56, 5.93±2.1, 7.17±0.42, 7.38±1.03 and 15.72±1.18 μM, respectively), while compounds 1 and 7 showed lowest DPPH radical scavenging activity comparable with the standard trolox (IC50: 5.93±2.19 M).
Animals fed with a high amount of a wide range of antioxidants in their diet are significantly pr... more Animals fed with a high amount of a wide range of antioxidants in their diet are significantly protected against oxidative stress. Powerful antioxidant substances such as vitamin E, vitamin C, and carotenoids are present naturally in red-hot pepper (RHP). This study hypothesized that using RHP may provide protection against oxidative stress and enhance animal physiological responses. Thus, this study aimed to investigate the effect of feeding New Zealand white rabbits with RHP-supplemented diets on their physiological and biochemical responses. New Zealand White rabbits (age = 6 weeks, n = 48) were split equally into three groups (n = 16 in each group). One group was fed a basal diet only (control group), with the other two groups fed a basal diet along with 1 and 2% RHP. Mass spectrometric analysis for the RHP methanolic extract showed some phenolic compounds, such as p-coumaric, sinapinic acids, vanillic, and luteolin, as well as catechin and its isomers. Hepatic antioxidant enzym...
Journal of the Egyptian Society of Parasitology, 2016
1,4-Dioxane (DX) with two oxygen atoms make it hydrophilic and infinitely soluble in water. As a ... more 1,4-Dioxane (DX) with two oxygen atoms make it hydrophilic and infinitely soluble in water. As a synthetic organic compound, it used widely throughout industry as a solvent. Dioxane causes numerous human ailments such as liver damage and kidney failure. It has been shown in research to be carcinogenic to animals, and is a potential carcinogen to humans. Daily administration for 1,4-dioxane (100 mg/kg body weight) in drinking water for rats weighing 120 g, except for normal control group. Experimental animal for 42 days was followed through body weight, serum alkaline phosphatase, serum creatinine, malondialdehyde, and catalase enzyme activity; beside histological patterns for liver, kidney, brain and ovary sections. Protection treatment has been offered using oral injection N-acetyl cysteine (100 mg/kg b.wt.), and fresh 200 mg/kg b.wt. in diet meal for each of nabk, husk, and sycamore in separated groups. Body weight and CAT activity have decreased by 25.8, and 68.7%, respectively. ...
Uploads
Papers by emad shaker