High-performance capillary electrophoresis (CE) with electrochemical detection (ED) was employed ... more High-performance capillary electrophoresis (CE) with electrochemical detection (ED) was employed to determine hydroxyl radicals in the Fenton reaction. Hydroxyl radicals can react with salicylic acid to produce 2,3-dihydroxy benzoic acid and 2,5-dihydroxy benzoic acid, which can be analyzed by CE-ED. Based on this principle, hydroxyl radicals were determined indirectly. In a 20 mmol/L phosphate running buffer (pH 7.4), 2,3-dihydroxy benzoic acid and 2,5-dihydroxy benzoic acid would elute simultaneously from the capillary within 6 min. As the working electrode, a 300 μm diameter carbon-disk electrode exhibits good responses at +0.60 V (vs. SCE) for the two analytes. Peak currents of the two analytes are additive. Excellent linearity was obtained in the concentration range from 1.0×10-3 mol/L to 5.0×10-6 mol/L for 2,3-dihydroxy benzoic acid. The detection limit (S/N=3) was 2.0×10-6 mol/L. This method was successfully applied for studying hydroxyl radical scavenging activities of Chinese herbs. It is testified that Apocynum Venetum L., Jinkgo bibola L., Morus alba L. and Rhododendron dauricum L. have strong hydroxyl radical scavenging activities.
Mulberry (Morus L) is one of the economically important trees that have a long history of extensi... more Mulberry (Morus L) is one of the economically important trees that have a long history of extensive cultivation in Asia. Mulberry leaf is the sole food for the silkworm Bombyx mori; hence, the sustainability of the sericulture industry is dependent on the continuous supply of nutritious mulberry leaf. Genetic improvement of mulberry for leaf yield, leaf nutritional contents, and adaptability has great socioeconomical importance because the sericulture is the backbone of rutal economy in several Asian countries. Much effort has been made on germplasm collection, characterization, and varietal development through conventional methods. However, the complexity of important agronomic traits, the substantial lag time between germination and sexual maturity, the absence of inbred lines, and the outbreeding nature of mulberry delay the genetic improvement via conventional breeding techniques. In this postgenomic era, having the next generation sequencing facilities in its fold, it is possible to integrate modern genomic tools with conventional breeding techniques to dissect the complex traits into their individual components to gain better control over them. In mulberry, such efforts are lacking. This paper, therefore, summarizes the current position of genetic and genomics research in mulberry and discusses the directions for future research, utilizing the emerging technologies in molecular markers, association genetics, quantitative trait locus mapping, transcriptomics, metabolomics, marker-assisted selection breeding, and transgenesis.
Page 1. Book reviews 99 expected a French publisher. But this would be to suppose publishers and ... more Page 1. Book reviews 99 expected a French publisher. But this would be to suppose publishers and a higher educational establishment in France which accorded to history of science an appreciable fraction of the recognition which they give to philosophy. ...
High-performance capillary electrophoresis (CE) with electrochemical detection (ED) was employed ... more High-performance capillary electrophoresis (CE) with electrochemical detection (ED) was employed to determine hydroxyl radicals in the Fenton reaction. Hydroxyl radicals can react with salicylic acid to produce 2,3-dihydroxy benzoic acid and 2,5-dihydroxy benzoic acid, which can be analyzed by CE-ED. Based on this principle, hydroxyl radicals were determined indirectly. In a 20 mmol/L phosphate running buffer (pH 7.4), 2,3-dihydroxy benzoic acid and 2,5-dihydroxy benzoic acid would elute simultaneously from the capillary within 6 min. As the working electrode, a 300 μm diameter carbon-disk electrode exhibits good responses at +0.60 V (vs. SCE) for the two analytes. Peak currents of the two analytes are additive. Excellent linearity was obtained in the concentration range from 1.0×10-3 mol/L to 5.0×10-6 mol/L for 2,3-dihydroxy benzoic acid. The detection limit (S/N=3) was 2.0×10-6 mol/L. This method was successfully applied for studying hydroxyl radical scavenging activities of Chinese herbs. It is testified that Apocynum Venetum L., Jinkgo bibola L., Morus alba L. and Rhododendron dauricum L. have strong hydroxyl radical scavenging activities.
Mulberry (Morus L) is one of the economically important trees that have a long history of extensi... more Mulberry (Morus L) is one of the economically important trees that have a long history of extensive cultivation in Asia. Mulberry leaf is the sole food for the silkworm Bombyx mori; hence, the sustainability of the sericulture industry is dependent on the continuous supply of nutritious mulberry leaf. Genetic improvement of mulberry for leaf yield, leaf nutritional contents, and adaptability has great socioeconomical importance because the sericulture is the backbone of rutal economy in several Asian countries. Much effort has been made on germplasm collection, characterization, and varietal development through conventional methods. However, the complexity of important agronomic traits, the substantial lag time between germination and sexual maturity, the absence of inbred lines, and the outbreeding nature of mulberry delay the genetic improvement via conventional breeding techniques. In this postgenomic era, having the next generation sequencing facilities in its fold, it is possible to integrate modern genomic tools with conventional breeding techniques to dissect the complex traits into their individual components to gain better control over them. In mulberry, such efforts are lacking. This paper, therefore, summarizes the current position of genetic and genomics research in mulberry and discusses the directions for future research, utilizing the emerging technologies in molecular markers, association genetics, quantitative trait locus mapping, transcriptomics, metabolomics, marker-assisted selection breeding, and transgenesis.
Page 1. Book reviews 99 expected a French publisher. But this would be to suppose publishers and ... more Page 1. Book reviews 99 expected a French publisher. But this would be to suppose publishers and a higher educational establishment in France which accorded to history of science an appreciable fraction of the recognition which they give to philosophy. ...
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