Papers by bhuvaneswari chodisetti
Applied Biochemistry and Biotechnology, May 17, 2016
Abutilon indicum exploited for its immense value has been propagated successfully through multipl... more Abutilon indicum exploited for its immense value has been propagated successfully through multiple shoot induction and somatic embryogenesis. Direct regeneration (8.20 ± 0.83 shoots) was achieved from nodal explants using 0.5 mg/l kinetin (Kn) in MS media. The basal callus from nodal explants turned embryogenic on subsequent introduction of 0.2 mg/l TDZ into the Kn-supplemented media, giving rise to somatic embryos. The embryogenic potential of calli expressed in terms of embryo-forming capacity (EFC) increased from 8.15 EFC to 20.95 EFC after plasmolysis. The phytochemical analysis (HPLC) for the presence of scopoletin and scoparone has revealed a unique accumulation pattern, with higher levels of scopoletin during the earlier stages and scoparone in the later stages of development. The embryogenic calli contained the highest amount of coumarins (99.20 ± 0.97 and 61.03 ± 0.47 μg/gFW, respectively) followed by regenerated plant (9.43 ± 0.20 and 36.36 ± 1.19 μg/gFW, respectively), obtained via somatic embryogenesis. Rapid multiplication of A. indicum equipped with two potent coumarins is important in order to meet the commercial demand for combat against dreadful diseases, thereby providing a new platform for plant-based drugs and their manufacture on a commercial scale.
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Applied Biochemistry and Biotechnology, Jul 14, 2012
Agrobacterium-mediated transformations ensure elevated amounts of secondary metabolite accumulati... more Agrobacterium-mediated transformations ensure elevated amounts of secondary metabolite accumulation with genetic and biosynthetic stability. In the present study, Alpinia galanga rich in bioactive compounds was genetically transformed using different strains of Agrobacterium rhizogenes viz. LBA 9402, A(4), 532, 2364 and PRTGus. Even though a higher growth rate was obtained with the LBA 9402 strain, maximum acetoxychavicol acetate accumulation (ACA) was seen in the PRTGus transformant. PRTGus root line has shown 10.1 fold higher ACA content in comparison to the control roots. The lowest ACA production was shown by the A(4) transformant (4.9 fold). The quantification of ACA in the transformed roots was carried out by using HPLC, which was found to be in the order of PRTGus > LBA 9402 > 2364 > 532 > A(4). The fast growth rate of hairy roots, genetic stability and their ability to synthesize more than one metabolite offer a promising system for the production of valuable secondary metabolites.
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Applied Biochemistry and Biotechnology, Sep 3, 2011
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Applied Biochemistry and Biotechnology, Sep 23, 2012
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Plant Biosystems, Mar 15, 2021
Abutilon indicum (Malvaceae), a therapeutically valuable shrub can act as a continuous source of ... more Abutilon indicum (Malvaceae), a therapeutically valuable shrub can act as a continuous source of stigmasterol, accredited with pharmacological significance. In the present study, the content of sti...
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International journal of scientific research in biological science, Oct 31, 2018
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Natural Product Research, Mar 1, 2013
Gymnema sylvestre (CS 149), known to be a rich source of saponins and other valuable phytochemica... more Gymnema sylvestre (CS 149), known to be a rich source of saponins and other valuable phytochemicals, has been analysed for antimicrobial activity. The chloroform extracts of aerial and root parts of G. sylvestre exhibited higher antimicrobial activity as compared to diethyl ether and acetone. The root extracts of chloroform have shown competitive minimum inhibitory concentration and minimum bactericidal concentration values in the range of 0.04-1.28 mg mL(-1) and 0.08-2.56 mg/mL, respectively, towards the pathogens. The GC-MS analysis of chloroform extracts has shown the presence of compounds like eicosane, oleic acid, stigmasterol and vitamin E.
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Plant Biotechnology Reports, Jun 27, 2013
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Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, 2021
Abutilon indicum (Malvaceae), a therapeutically valuable shrub can act as a continuous source of ... more Abutilon indicum (Malvaceae), a therapeutically valuable shrub can act as a continuous source of stigmasterol, accredited with pharmacological significance. In the present study, the content of sti...
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Applied Biochemistry and Biotechnology, 2016
Abutilon indicum exploited for its immense value has been propagated successfully through multipl... more Abutilon indicum exploited for its immense value has been propagated successfully through multiple shoot induction and somatic embryogenesis. Direct regeneration (8.20 ± 0.83 shoots) was achieved from nodal explants using 0.5 mg/l kinetin (Kn) in MS media. The basal callus from nodal explants turned embryogenic on subsequent introduction of 0.2 mg/l TDZ into the Kn-supplemented media, giving rise to somatic embryos. The embryogenic potential of calli expressed in terms of embryo-forming capacity (EFC) increased from 8.15 EFC to 20.95 EFC after plasmolysis. The phytochemical analysis (HPLC) for the presence of scopoletin and scoparone has revealed a unique accumulation pattern, with higher levels of scopoletin during the earlier stages and scoparone in the later stages of development. The embryogenic calli contained the highest amount of coumarins (99.20 ± 0.97 and 61.03 ± 0.47 μg/gFW, respectively) followed by regenerated plant (9.43 ± 0.20 and 36.36 ± 1.19 μg/gFW, respectively), obtained via somatic embryogenesis. Rapid multiplication of A. indicum equipped with two potent coumarins is important in order to meet the commercial demand for combat against dreadful diseases, thereby providing a new platform for plant-based drugs and their manufacture on a commercial scale.
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In Vitro Cellular & Developmental Biology – Plant, Dec 9, 2014
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International Journal of Scientific Research in Biological Sciences, 2018
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In Vitro Cellular & Developmental Biology - Plant, 2014
ABSTRACT Gymnema sylvestre is a natural source of gymnemic acids—a group of closely related trite... more ABSTRACT Gymnema sylvestre is a natural source of gymnemic acids—a group of closely related triterpenoid saponins used in the treatment of diabetes. The present study is aimed at enhancing gymnemic acid production in G. sylvestre cell suspension cultures using the signaling molecules—methyl jasmonate and salicylic acid. Methyl jasmonate yielded the maximum gymnemic acid content of 135.41 ± 0.43 mg g−1 dry cell weight (DCW), 72 h after elicitor application. Salicylic acid, on the other hand, evoked a moderate response reaching a maximum of 43.27 ± 0.80 mg g−1 DCW, 48 h after application. The results of the present study increase the possibility for commercial production of gymnemic acids on a large scale using G. sylvestre cell suspensions.
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Plant Biotechnology Reports, 2013
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Natural Product Research, 2013
Gymnema sylvestre (CS 149), known to be a rich source of saponins and other valuable phytochemica... more Gymnema sylvestre (CS 149), known to be a rich source of saponins and other valuable phytochemicals, has been analysed for antimicrobial activity. The chloroform extracts of aerial and root parts of G. sylvestre exhibited higher antimicrobial activity as compared to diethyl ether and acetone. The root extracts of chloroform have shown competitive minimum inhibitory concentration and minimum bactericidal concentration values in the range of 0.04-1.28 mg mL(-1) and 0.08-2.56 mg/mL, respectively, towards the pathogens. The GC-MS analysis of chloroform extracts has shown the presence of compounds like eicosane, oleic acid, stigmasterol and vitamin E.
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Applied Biochemistry and Biotechnology, 2012
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Applied Biochemistry and Biotechnology, 2011
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Applied Biochemistry and Biotechnology, 2012
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Papers by bhuvaneswari chodisetti