Papers by Viviane Botosso
Talanta, 2023
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<p>Bayesian skyline plots of HRSV genotypes prevalent in São Paulo (top) and seasonal distr... more <p>Bayesian skyline plots of HRSV genotypes prevalent in São Paulo (top) and seasonal distribution of HRSV cases in São Paulo during the 1995–2005 seasons are shown in the <i>x</i>-axis. GA1, GA3 GA7 and GB4 were excluded from the BSL analysis because of small sample size (n<10). The <i>y</i>-axis (on the left) represents a measure of relative genetic diversity presented as <i>Ne.g</i> reflecting the change in effective number of infections over time; where <i>g</i> is the average generation time. The <i>y</i>-axis (on the right) represents the number of samples in the study. (-) - Number of total samples collected during the period. (-) - Number of all HRSV positive cases identified with monoclonal antibodies and molecular characterization.</p
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Human Virology in Latin America, 2017
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<p>Bayesian skyline plots of complete HRSV sequences of HRSVA (n = 1203) and HRSVB (n = 780... more <p>Bayesian skyline plots of complete HRSV sequences of HRSVA (n = 1203) and HRSVB (n = 780). The <i>y</i>-axis represents a measure of relative genetic diversity presented as <i>Ne.g</i> reflecting the change in effective population (a surrogate for number of infections) over time for the complete set of HRSV sequences for HRSVA (n = 1204) and HRSVB (n = 778). The dotted lines define the likelihood bounds corresponding to a 95% confidence interval (CI). (…) - Upper and lower limits for HRSVA; (—) - Upper and lower limits for HRSVB. The arrow represents a shift in dynamics between HRSVA and HRSVB.</p
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<p>A) Bayesian skyline plots of HRSVA genotypes B) Bayesian skyline plots of HRSVB genotype... more <p>A) Bayesian skyline plots of HRSVA genotypes B) Bayesian skyline plots of HRSVB genotypes. Positively selected amino acid substitution sites are represented as previously described by Botosso et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0041953#pone.0041953-Botosso1" target="_blank">[48]</a>. The <i>y</i>-axis represents a measure of relative genetic diversity presented as <i>Ne.g</i> reflecting the change in effective number of infections over time.</p
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Background: Human respiratory syncytial virus (HRSV) is one of the major etiologic agents of resp... more Background: Human respiratory syncytial virus (HRSV) is one of the major etiologic agents of respiratory tract infections among children worldwide. Methodology/Principal Findings: Here through a comprehensive analysis of the two major HRSV groups A and B (n = 1983) which comprise of several genotypes, we present a complex pattern of population dynamics of HRSV over a time period of 50 years (1956–2006). Circulation pattern of HRSV revealed a series of expansions and fluctuations of co-circulating lineages with a predominance of HRSVA. Positively selected amino acid substitutions of the G glycoprotein occurred upon population growth of GB3 with a 60-nucleotide insertion (GB3 Insert), while other genotypes acquired substitutions upon both population growth and decrease, thus possibly reflecting a role for immune selected epitopes in linkage to the traced substitution sites that may have important relevance for vaccine design. Analysis evidenced the co-circulation and predominance of d...
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Human Virology in Latin America, 2017
According to the World Health Organization, acute lower respiratory tract infection (ALRI) is the... more According to the World Health Organization, acute lower respiratory tract infection (ALRI) is the fourth leading cause of death worldwide, responsible for approximately 3.1 million (5.5%) of the 56 million estimated deaths per year. Human respiratory syncytial virus (RSV) is recognized as the most important cause of ALRI in infants worldwide. It is estimated that every year 33.8 million new cases of RSV-related ALRIs occur and cause approximately 200,000 deaths in children younger than 5 years; of note, 99% of these deaths occur in developing countries. Usually primary infections occur during the first 2 years of life and are symptomatic, causing from mild upper respiratory tract illness to severe ALRI, and in some cases pneumonia and bronchiolitis may occur simultaneously. Recurrent infections throughout life are common but normally cause milder upper respiratory tract illness. Several studies also associated severe RSV infections during infancy with permanent lung function reducti...
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PLOS Neglected Tropical Diseases, 2021
This study aims to describe the sociodemographic determinants associated with exposure to Zika Vi... more This study aims to describe the sociodemographic determinants associated with exposure to Zika Virus (ZIKV) in pregnant women during the 2015–2016 epidemic in Salvador, Brazil. Methods We recruited women who gave birth between October 2015 and January 2016 to a cross-sectional study at a referral maternity hospital in Salvador, Brazil. We collected information on their demographic, socioeconomic, and clinical characteristics, and evaluated their ZIKV exposure using a plaque reduction neutralization test. Logistic regression was then used to assess the relationship between these social determinants and ZIKV exposure status. Results We included 469 pregnant women, of whom 61% had a positive ZIKV result. Multivariate analysis found that lower education (adjusted Prevalence Rate [aPR] 1.21; 95%CI 1.04–1.35) and food insecurity (aPR 1.17; 95%CI 1.01–1.30) were positively associated with ZIKV exposure. Additionally, age was negatively associated with the infection risk (aPR 0.99; 95%CI 0....
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Life, 2021
Biopharmaceutical production is currently a multibillion-dollar industry with high growth perspec... more Biopharmaceutical production is currently a multibillion-dollar industry with high growth perspectives. The research and development of biologically sourced pharmaceuticals are extremely important and a reality in our current healthcare system. Interferon alpha consensus (cIFN) is a non-natural synthetic antiviral molecule that comprises all the most prevalent amino acids of IFN-α into one consensus protein sequence. For clinical use, cIFN is produced in E. coli in the form of inclusion bodies. Here, we describe the use of two solubility tags (Fh8 and DsbC) to improve soluble cIFN production. Furthermore, we analyzed cIFN production in different culture media and temperatures in order to improve biopharmaceutical production. Our results demonstrate that Fh8-cIFN yield was improved when bacteria were cultivated in autoinduction culture medium at 30 °C. After hydrolysis, the recovery of soluble untagged cIFN was 58% from purified Fh8-cIFN molecule, fourfold higher when compared to cIF...
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Antibodies, 2017
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Viruses, 2018
We followed the presence of Zika virus (ZIKV) in four healthy adults (two men and two women), for... more We followed the presence of Zika virus (ZIKV) in four healthy adults (two men and two women), for periods ranging from 78 to 298 days post symptom onset. The patients were evaluated regarding the presence of the virus in different body fluids (blood, saliva, urine and semen), development of immune responses (including antibodies, cytokines and chemokines), and virus genetic variation within samples collected from semen and urine during the infection course. The analysis was focused primarily on the two male patients who shed the virus for up to 158 days after the initial symptoms. ZIKV particles were detected in the spermatozoa cytoplasm and flagella, in immature sperm cells and could also be isolated from semen in cell culture, confirming that the virus is able to preserve integrity and infectivity during replication in the male reproductive system (MRS). Despite the damage caused by ZIKV infection within the MRS, our data showed that ZIKV infection did not result in infertility at...
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Emerging infectious diseases, Jan 15, 2018
Yellow fever virus RNA is usually detected in blood of infected humans. We detected virus RNA in ... more Yellow fever virus RNA is usually detected in blood of infected humans. We detected virus RNA in urine and semen samples from a convalescent patient. A complete virus genome was sequenced for an isolate from a urine sample. This virus had a South American I genotype and unique synapomorphic changes.
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New England Journal of Medicine, 2016
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Nanomedicine : nanotechnology, biology, and medicine, Nov 20, 2016
Due to its physicochemical properties, nanostructured mesoporous SBA-15 silica shows great potent... more Due to its physicochemical properties, nanostructured mesoporous SBA-15 silica shows great potential as a vaccine adjuvant. This study evaluated the capacity of SBA-15 to encapsulate/adsorb the recombinant purified HBsAg from the Hepatitis B virus and the immunoresponsiveness of mice orally immunized with HBsAg inside SBA-15. A simulation of small angle X-ray scattering experimental results, together with the nitrogen adsorption isotherms data, allowed to determine the appropriate mass ratio of HBsAg:SBA-15, indicating antigen encapsulation into SBA-15 macroporosity. This was also evaluated by bicinchoninic acid assay and gel electrophoresis. The recruitment of inflammatory cells, an increase in production of specific antibodies, and the non-influence of silica on TH1 or TH2 polarization were observed after oral immunization. Besides, SBA-15 enhanced the phagocytosis of ovalbumin by dendritic cells, an important key to prove how this adjuvant works. Thus, it seems clear that the nan...
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VIRUS Reviews & Research, 2000
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PLoS Pathogens, 2009
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Journal of Virological Methods, 2008
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The European Physical Journal Special Topics, 2019
Abstract As a consequence of its ordered pore architecture, mesoporous SBA-15 offers new possibil... more Abstract As a consequence of its ordered pore architecture, mesoporous SBA-15 offers new possibilities for incorporating biological agents. Considering its applicability in oral vaccination, which shows more beneficial features when compared with parenteral vaccines, SBA-15 is also seen as a very promising adjuvant to carry, protect, and deliver entrapped antigens. Recent studies have shown several remarkable features in the immunization of hepatitis B, a viral disease transmitted mainly through blood or serum transfer. However, the surface antigen of the hepatitis B virus, HBsAg, is too large to fit inside the SBA-15 matrix with mean pore diameter around 10 nm, thus raising the question of how SBA-15 can protect the antigen. In this work, thermal analysis combined with neutron spectroscopy allowed us to shed light on the interactions between HBsAg and SBA-15 as well as on the role that these interactions play in the efficiency of this promising oral vaccination method. This information was obtained by verifying how the dynamic behaviour of the antigen is modified under confinement in SBA-15, thus also establishing an experimental method for verifying molecular dynamics simulations.
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Viral upper respiratory tract infections (URTI) have been correlated with the onset of asthma att... more Viral upper respiratory tract infections (URTI) have been correlated with the onset of asthma attacks in children and viral identification was reported in 14-49 % of nasal samples. The aim of the present study was to detect influenza, parainfluenza, adenovirus and respiratory syncytial virus (RSV) in older children during acute asthma attacks. A total of 104 children (2-14 years) were included in four groups: group I: asthmatics with acute attack and URTI; group II: asthmatics without URTI (group I children, 30 days later); group III: non-asthmatics with URTI; group IV: non-asthmatic, asymptomatic children. A diagnosis of URTI was considered when (3 symptoms (cough and/or sneeze, nasal obstruction, hypertrophy of turbinates, pain and/or retropharynx hyperemia, headache and fever) in asthmatics and at least 2 symptoms in non-asthmatics were present, starting within 7 days. Samples of nasal mucosa cells (n = 123) were collected, and culture and indirect immunofluorescence were carried out to identify respiratory syncytial virus, adenovirus, influenza A and B, parainfluenza 1,2 and 3 and rhinovirus. Viral identification rates were higher in the asthmatic groups: 13.9 % in group I, 11.1 % in group II; 2.8 % in group III and 0 in group IV. The following viruses were identified: RSV 2/36, rhinovirus 1/36, adenovirus 1/36 and parainfluenzae 1/36 in group I; adenovirus 2/18 in group II; RSV 1/36 in group III. The rate of viral identification was higher in asthmatic children, whether symptomatic or not, suggesting a possible susceptibility to viral infections. Virus could also be a triggering factor in attacks, although it is not the most preponderant in older children.
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Papers by Viviane Botosso