Extracellular alkaline protease produced by an isolate fromsoil was preliminarily identified to b... more Extracellular alkaline protease produced by an isolate fromsoil was preliminarily identified to be Bacillus subtilis. Studies on submerged fermentation revealed that maximum level i.e. 321 µg/ml/min of protease production was observed during early stationary phase. Optimization of fermentation mediumfor characterization of protease was carried out. Optimization parameter includes incubation period, temperature, pH, substrate concentration, carbon sources, nitrogen sources and tween 80 concentrations. The optimized conditions found for protease production were 400C at pH 10 (alkaline condition), with 10%inoculums, 2 ml tween 80, 1%sucrose, 1%ammoniumchloride and 20% casein, after 72 h of incubation stimulates protease production. The protease profile of the selected isolate shows its potential for its industrial applications.
Textile dyes (reactive red 195 and reactive green 11) were selected for biodegradation studies by... more Textile dyes (reactive red 195 and reactive green 11) were selected for biodegradation studies by Aspergillus niger grp using synthetic wastewater. Addition of glucose (1 g % w/v), ammonium sulphate (0.2 g % and 0.3 g % respectively for reactive red 195 and reactive green 11) favoured degradation. Optimum inoculum (size 1 g %, pH 7.0 and temp. 30 °C) was prepared for degradation of both [he dyes. Static conditions favoured dye decolarization than shaking conditions. Under optimized conditions, degradation by Aspergillus niger grp was found to be: reactive red 195, 93.0; and reactive green 11. 80 %. Degradation of dyes was confirmed by UV- Spectrophotometric and TLC analysis. This indigenous isolate could be a potential organism for developing an efficient and effective technology for bioremediation of textile wastewater effluents carrying these dyes.
Textile dyes (reactive red 195 and reactive green 11) were selected for biodegradation studies by... more Textile dyes (reactive red 195 and reactive green 11) were selected for biodegradation studies by Aspergillus niger grp using synthetic wastewater. Addition of glucose (1 g % w/v), ammonium sulphate (0.2 g % and 0.3 g % respectively for reactive red 195 and reactive green 11) favoured degradation. Optimum inoculum (size 1 g %, pH 7.0 and temp. 30 °C) was prepared for degradation of both the dyes. Static conditions favoured dye decolarization than shaking conditions. Under optimized conditions, degradation by Aspergillus niger grp was found to be: reactive red 195, 93.0; and reactive green 11, 80 %. Degradation of dyes was confirmed by UVSpectrophotometric and TLC analysis. This indigenous isolate could be a potential organism for developing an efficient and effective technology for bioremediation of textile wastewater effluents carrying these dyes.
Extracellular alkaline protease produced by an isolate fromsoil was preliminarily identified to b... more Extracellular alkaline protease produced by an isolate fromsoil was preliminarily identified to be Bacillus subtilis. Studies on submerged fermentation revealed that maximum level i.e. 321 µg/ml/min of protease production was observed during early stationary phase. Optimization of fermentation mediumfor characterization of protease was carried out. Optimization parameter includes incubation period, temperature, pH, substrate concentration, carbon sources, nitrogen sources and tween 80 concentrations. The optimized conditions found for protease production were 400C at pH 10 (alkaline condition), with 10%inoculums, 2 ml tween 80, 1%sucrose, 1%ammoniumchloride and 20% casein, after 72 h of incubation stimulates protease production. The protease profile of the selected isolate shows its potential for its industrial applications.
Textile dyes (reactive red 195 and reactive green 11) were selected for biodegradation studies by... more Textile dyes (reactive red 195 and reactive green 11) were selected for biodegradation studies by Aspergillus niger grp using synthetic wastewater. Addition of glucose (1 g % w/v), ammonium sulphate (0.2 g % and 0.3 g % respectively for reactive red 195 and reactive green 11) favoured degradation. Optimum inoculum (size 1 g %, pH 7.0 and temp. 30 °C) was prepared for degradation of both [he dyes. Static conditions favoured dye decolarization than shaking conditions. Under optimized conditions, degradation by Aspergillus niger grp was found to be: reactive red 195, 93.0; and reactive green 11. 80 %. Degradation of dyes was confirmed by UV- Spectrophotometric and TLC analysis. This indigenous isolate could be a potential organism for developing an efficient and effective technology for bioremediation of textile wastewater effluents carrying these dyes.
Textile dyes (reactive red 195 and reactive green 11) were selected for biodegradation studies by... more Textile dyes (reactive red 195 and reactive green 11) were selected for biodegradation studies by Aspergillus niger grp using synthetic wastewater. Addition of glucose (1 g % w/v), ammonium sulphate (0.2 g % and 0.3 g % respectively for reactive red 195 and reactive green 11) favoured degradation. Optimum inoculum (size 1 g %, pH 7.0 and temp. 30 °C) was prepared for degradation of both the dyes. Static conditions favoured dye decolarization than shaking conditions. Under optimized conditions, degradation by Aspergillus niger grp was found to be: reactive red 195, 93.0; and reactive green 11, 80 %. Degradation of dyes was confirmed by UVSpectrophotometric and TLC analysis. This indigenous isolate could be a potential organism for developing an efficient and effective technology for bioremediation of textile wastewater effluents carrying these dyes.
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