Pseudomonas aeruginosa is associated with otitis and pyoderma in dogs and is frequently resistant... more Pseudomonas aeruginosa is associated with otitis and pyoderma in dogs and is frequently resistant to several antimicrobial drugs. Resistance genes can be carried by integrons with quinolone resistance mainly due to mutations in DNA topoisomerases II and IV. To evaluate the antimicrobial susceptibility, integron carriage, and gyrA and gyrB mutations in P. aeruginosa isolates from canine otitis and pyoderma. One hundred and four P. aeruginosa strains isolated from dogs with otitis externa (n = 93) and pyoderma (n = 11). Antimicrobial susceptibility against 16 antibacterial agents was evaluated through agar diffusion tests. Integron carriage, class and gyrA and gyrB mutations were analysed by PCR, restriction fragment length polymorphism (RFLP)-PCR and genetic sequencing assays. Isolates were mostly resistant to enrofloxacin (72.2%) and ticarcillin (59.7%). Lower resistance to ciprofloxacin (7.7%), tobramycin (3.8%) and polymixin B (0.0%) was detected. Ten (9.6%) multidrug-resistant (M...
Background Escherichia coli serogroup O25b-sequence type 131 (E. coli O25-B2-ST131) is considered... more Background Escherichia coli serogroup O25b-sequence type 131 (E. coli O25-B2-ST131) is considered as multidrug-resistant and hypervirulent organism. There is lack of data about involvement of this pathogen in the children’s infection. In this study, the prevalence, and clonality, virulence capacity, and antibiotic resistance phenotype and genotype of E. coli O25-B2-ST131 compared with non-O25-B2-ST131 isolates were investigated in children with urinary tract infection in Tehran, Iran. Methods The E. coli isolates from urine samples were identified using conventional microbiological methods. Characterization of E. coli O25-B2-ST131 clone, antibiotic susceptibility, biofilm formation, ESBLs phenotype and genotype, serum resistance, hemolysis, hydrophobicity, and formation of curli fimbriae were done using conventional microbiological and molecular methods. Clonality of the isolates was done by rep-PCR typing. Results Among 120 E. coli isolates, the highest and lowest antibiotic resist...
The human T-cell leukemia virus type 1 (HTLV-1) is a positive single-stranded RNA virus that belo... more The human T-cell leukemia virus type 1 (HTLV-1) is a positive single-stranded RNA virus that belongs to the delta retrovirus family. As a result, a vaccine candidate that can be recognized by B cells and T cells is a good candidate for generating a durable immune response. Further, the GPEHT protein is a multi-epitope protein designed based on the Gag, Pol, Env, Hbz, and Tax proteins of HTLV-1. In developing a suitable and effective vaccine against HTLV-1, the selection of a designed protein (GPEHT) with the formulation of an alum adjuvant was conducted. In this study, we assessed the potential of a multi-epitope vaccine candidate for stimulating the immune response against HTLV-1. In assessing the type of stimulated immune reaction, total IgG, IgG1, and IgG2a isotypes, as well as the cytokines associated with Th1 (IFN-γ), Th2 (IL-4), and Th17 (IL-17), were analyzed. The outcomes showed that the particular antisera (total IgG) were more elevated in mice that received the GPEHT prote...
The initial step in molecular biology and genetic engineering fields are the finding of optimal m... more The initial step in molecular biology and genetic engineering fields are the finding of optimal methods for the extraction of the genomic DNA from microorganisms and cells. There are several methods for the extraction of bacterial DNA and the use of magnetic nanoparticles is a novel method for the isolation of nucleic acid. In the present study, the synthesized magnetic nanoparticles were used to extract the genomic DNA of Escherichia coli. In this study, the naked (Fe3O4) and coated (Fe3O4/SiO2; Fe3O4/SiO2/TiO2) magnetic nanoparticles were synthesized by precipitation methods. The purity of the product was assessed using energy-dispersive x-ray spectroscopy (EDS) and x-ray crystallography. The morphology and size of specimens were observed via field emission scanning electron microscopy (FESEM). The efficacy of the synthesized nanoparticles in genomic DNA extraction from Escherichia coli strain ATCC25922 was compared. The quality of the resulting DNA was analyzed by NanoDrop spectr...
Pertussis caused by <i>Bordetella pertussis,</i> remains a public health problem worl... more Pertussis caused by <i>Bordetella pertussis,</i> remains a public health problem worldwide, despite high vaccine coverage in infants and children in many countries. Iran has been using whole cell vaccine for the last 50 years with more than 95% vaccination rate since 1988 and has experienced pertussis resurgence in recent years. Here, we sequenced 55 <i>B. pertussis</i> isolates mostly collected from three provinces with the highest number of pertussis cases in Iran, including Tehran, Mazandaran, and Eastern-Azarbayjan from the period of 2008-2016. Most isolates carried <i>ptxP3</i>/<i>prn2</i> alleles (42/55, 76%), the same genotype as isolates circulating in acellular vaccine-administrating countries. The second most frequent genotype was <i>ptxP3</i>/<i>prn9</i> (8/55, 14%). Only three isolates (5%) were <i>ptxP1</i>. Phylogenetic analysis showed that Iranian <i>ptxP3</i> isolates can ...
Journal of Babol University of Medical Sciences, 2017
J Babol Univ Med Sci; 19(2); Feb 2017; PP: 20-5 Received: Jul 24 2016, Revised: Nov 26 2016, Acce... more J Babol Univ Med Sci; 19(2); Feb 2017; PP: 20-5 Received: Jul 24 2016, Revised: Nov 26 2016, Accepted: Jan 15 2017. ABSTRACT BACKGROUND AND OBJECTIVE: Whooping cough is an acute contagious human respiratory disease especially in infants and children and is one of the ten major causes of death from infectious diseases. Despite reducing the risk due to vaccination with killed bacteria pathogen (Bordetella pertussis), increased disease emerged in recent decades indicates re-emerging due caused by genetic diversity of bacteria and the insufficiency of current vaccines. Therefore, the design of new and effective vaccines based on common strains of the population is essential. In this study the inactive recombinant protein of most important bacterial antigens (pertosis toxin, PTX), the most commonly pathogenic strain in Iran, were produced on the basis of the new generation of non-cellular vaccines. METHODS: Bacterial strain used in this study to determine the genome and gene amplificatio...
Brevundinomas diminuta is known as an opportunistic pathogen and is rarely associated with invasi... more Brevundinomas diminuta is known as an opportunistic pathogen and is rarely associated with invasive infections in humans causing infection in the different parts of the body. In this study, we identified three B. diminuta isolates from three patients with an initial diagnosis of pertussis. Isolates were confirmed using biochemical tests and 16s rRNA sequencing. All isolates were resistant to three different classes of antimicrobial drugs including ceftazidime and ciprofloxacin. We performed Illumina whole-genome sequencing for two isolates. The results showed an average genome size of 3.25 Mbp with the G+C content 67% with multiple predicted virulence factors and genes leading to antibiotic resistance. We found an open pan-genome with 1502 core genes by analysing 13 available global B. diminuta isolated from different environments such as water, soil, or gentamicin fermentation residue. In the phylogenetic analysis, our isolates were grouped with B. diminuta isolate collected from a...
Pseudomonas aeruginosa is associated with otitis and pyoderma in dogs and is frequently resistant... more Pseudomonas aeruginosa is associated with otitis and pyoderma in dogs and is frequently resistant to several antimicrobial drugs. Resistance genes can be carried by integrons with quinolone resistance mainly due to mutations in DNA topoisomerases II and IV. To evaluate the antimicrobial susceptibility, integron carriage, and gyrA and gyrB mutations in P. aeruginosa isolates from canine otitis and pyoderma. One hundred and four P. aeruginosa strains isolated from dogs with otitis externa (n = 93) and pyoderma (n = 11). Antimicrobial susceptibility against 16 antibacterial agents was evaluated through agar diffusion tests. Integron carriage, class and gyrA and gyrB mutations were analysed by PCR, restriction fragment length polymorphism (RFLP)-PCR and genetic sequencing assays. Isolates were mostly resistant to enrofloxacin (72.2%) and ticarcillin (59.7%). Lower resistance to ciprofloxacin (7.7%), tobramycin (3.8%) and polymixin B (0.0%) was detected. Ten (9.6%) multidrug-resistant (M...
Background Escherichia coli serogroup O25b-sequence type 131 (E. coli O25-B2-ST131) is considered... more Background Escherichia coli serogroup O25b-sequence type 131 (E. coli O25-B2-ST131) is considered as multidrug-resistant and hypervirulent organism. There is lack of data about involvement of this pathogen in the children’s infection. In this study, the prevalence, and clonality, virulence capacity, and antibiotic resistance phenotype and genotype of E. coli O25-B2-ST131 compared with non-O25-B2-ST131 isolates were investigated in children with urinary tract infection in Tehran, Iran. Methods The E. coli isolates from urine samples were identified using conventional microbiological methods. Characterization of E. coli O25-B2-ST131 clone, antibiotic susceptibility, biofilm formation, ESBLs phenotype and genotype, serum resistance, hemolysis, hydrophobicity, and formation of curli fimbriae were done using conventional microbiological and molecular methods. Clonality of the isolates was done by rep-PCR typing. Results Among 120 E. coli isolates, the highest and lowest antibiotic resist...
The human T-cell leukemia virus type 1 (HTLV-1) is a positive single-stranded RNA virus that belo... more The human T-cell leukemia virus type 1 (HTLV-1) is a positive single-stranded RNA virus that belongs to the delta retrovirus family. As a result, a vaccine candidate that can be recognized by B cells and T cells is a good candidate for generating a durable immune response. Further, the GPEHT protein is a multi-epitope protein designed based on the Gag, Pol, Env, Hbz, and Tax proteins of HTLV-1. In developing a suitable and effective vaccine against HTLV-1, the selection of a designed protein (GPEHT) with the formulation of an alum adjuvant was conducted. In this study, we assessed the potential of a multi-epitope vaccine candidate for stimulating the immune response against HTLV-1. In assessing the type of stimulated immune reaction, total IgG, IgG1, and IgG2a isotypes, as well as the cytokines associated with Th1 (IFN-γ), Th2 (IL-4), and Th17 (IL-17), were analyzed. The outcomes showed that the particular antisera (total IgG) were more elevated in mice that received the GPEHT prote...
The initial step in molecular biology and genetic engineering fields are the finding of optimal m... more The initial step in molecular biology and genetic engineering fields are the finding of optimal methods for the extraction of the genomic DNA from microorganisms and cells. There are several methods for the extraction of bacterial DNA and the use of magnetic nanoparticles is a novel method for the isolation of nucleic acid. In the present study, the synthesized magnetic nanoparticles were used to extract the genomic DNA of Escherichia coli. In this study, the naked (Fe3O4) and coated (Fe3O4/SiO2; Fe3O4/SiO2/TiO2) magnetic nanoparticles were synthesized by precipitation methods. The purity of the product was assessed using energy-dispersive x-ray spectroscopy (EDS) and x-ray crystallography. The morphology and size of specimens were observed via field emission scanning electron microscopy (FESEM). The efficacy of the synthesized nanoparticles in genomic DNA extraction from Escherichia coli strain ATCC25922 was compared. The quality of the resulting DNA was analyzed by NanoDrop spectr...
Pertussis caused by <i>Bordetella pertussis,</i> remains a public health problem worl... more Pertussis caused by <i>Bordetella pertussis,</i> remains a public health problem worldwide, despite high vaccine coverage in infants and children in many countries. Iran has been using whole cell vaccine for the last 50 years with more than 95% vaccination rate since 1988 and has experienced pertussis resurgence in recent years. Here, we sequenced 55 <i>B. pertussis</i> isolates mostly collected from three provinces with the highest number of pertussis cases in Iran, including Tehran, Mazandaran, and Eastern-Azarbayjan from the period of 2008-2016. Most isolates carried <i>ptxP3</i>/<i>prn2</i> alleles (42/55, 76%), the same genotype as isolates circulating in acellular vaccine-administrating countries. The second most frequent genotype was <i>ptxP3</i>/<i>prn9</i> (8/55, 14%). Only three isolates (5%) were <i>ptxP1</i>. Phylogenetic analysis showed that Iranian <i>ptxP3</i> isolates can ...
Journal of Babol University of Medical Sciences, 2017
J Babol Univ Med Sci; 19(2); Feb 2017; PP: 20-5 Received: Jul 24 2016, Revised: Nov 26 2016, Acce... more J Babol Univ Med Sci; 19(2); Feb 2017; PP: 20-5 Received: Jul 24 2016, Revised: Nov 26 2016, Accepted: Jan 15 2017. ABSTRACT BACKGROUND AND OBJECTIVE: Whooping cough is an acute contagious human respiratory disease especially in infants and children and is one of the ten major causes of death from infectious diseases. Despite reducing the risk due to vaccination with killed bacteria pathogen (Bordetella pertussis), increased disease emerged in recent decades indicates re-emerging due caused by genetic diversity of bacteria and the insufficiency of current vaccines. Therefore, the design of new and effective vaccines based on common strains of the population is essential. In this study the inactive recombinant protein of most important bacterial antigens (pertosis toxin, PTX), the most commonly pathogenic strain in Iran, were produced on the basis of the new generation of non-cellular vaccines. METHODS: Bacterial strain used in this study to determine the genome and gene amplificatio...
Brevundinomas diminuta is known as an opportunistic pathogen and is rarely associated with invasi... more Brevundinomas diminuta is known as an opportunistic pathogen and is rarely associated with invasive infections in humans causing infection in the different parts of the body. In this study, we identified three B. diminuta isolates from three patients with an initial diagnosis of pertussis. Isolates were confirmed using biochemical tests and 16s rRNA sequencing. All isolates were resistant to three different classes of antimicrobial drugs including ceftazidime and ciprofloxacin. We performed Illumina whole-genome sequencing for two isolates. The results showed an average genome size of 3.25 Mbp with the G+C content 67% with multiple predicted virulence factors and genes leading to antibiotic resistance. We found an open pan-genome with 1502 core genes by analysing 13 available global B. diminuta isolated from different environments such as water, soil, or gentamicin fermentation residue. In the phylogenetic analysis, our isolates were grouped with B. diminuta isolate collected from a...
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