Effect of gamma irradiation on the nutritional quality of Agaricus bisporus strains cultivated in... more Effect of gamma irradiation on the nutritional quality of Agaricus bisporus strains cultivated in different composts
Universidade Estadual Paulista, UNESP, Faculdade de Ciencias Agronomicas, FCA, Departamento de Pr... more Universidade Estadual Paulista, UNESP, Faculdade de Ciencias Agronomicas, FCA, Departamento de Producao Vegetal/Defesa Fitossanitaria, Modulo de Cogumelos. Rua Jose Barbosa de Barros, 1780 - Fazenda Lageado. Caixa Postal 237, 18610-307. Botucatu, SP, Brasil
Universidade Estadual Paulista (UNESP), Faculdade de Ciencias Agronomicas (FCA), Departamento de ... more Universidade Estadual Paulista (UNESP), Faculdade de Ciencias Agronomicas (FCA), Departamento de Producao e Melhoramento Vegetal, Botucatu, SP, Brasil
Universidade Estadual Paulista (UNESP), Faculdade de Ciencias Agronomicas de Botucatu, Departamen... more Universidade Estadual Paulista (UNESP), Faculdade de Ciencias Agronomicas de Botucatu, Departamento de Ciencias Florestais, Fazenda Experimental Lageado
Universidade Estadual Paulista (UNESP), Faculdade de Ciencias Agronomicas (FCA), Departamento de ... more Universidade Estadual Paulista (UNESP), Faculdade de Ciencias Agronomicas (FCA), Departamento de Ciencia Florestal, Botucatu, SP, Brasil
The inhibition of arginase from Leishmania spp. is considered a promising approach to the leishma... more The inhibition of arginase from Leishmania spp. is considered a promising approach to the leishmaniasis treatment. In this study, the potential of a fucogalactan isolated from the medicinal mushroom Agrocybe aegerita was evaluated against arginase (ARG) from Leishmania amazonensis. The polysaccharide was obtained via aqueous extraction, and purified by freeze thawing and precipitation with Fehling solution. Its chemical structure was established by monosaccharide composition, methylation analysis, partial acid hydrolysis, and NMR spectroscopy. The data indicated that it is a fucogalactan (FG-Aa; M = 13.8 kDa), having a (1→6)-linked α-D-Galp main-chain partially substituted in O-2 by non-reducing end-units of α-L-Fucp. FG-Aa showed significant inhibitory activity on ARG with ICpotency of 5.82 ± 0.57 μM. The mechanism of ARG inhibition by the heterogalactan was the competitive type, with Kof 1.54 ± 0.15 μM. This is the first report of an inhibitory activity of arginase from L. amazone...
Pleurotus citrinopileatus, popularly known as "golden oyster mushroom" have medicinal p... more Pleurotus citrinopileatus, popularly known as "golden oyster mushroom" have medicinal properties, which are attributed mainly to the presence of bioactive polysaccharides. In this work, two partially 3-O-methylated galactans were isolated from the fruiting bodies of this fungus, via successive aqueous extraction, followed by fractionation by freeze-thawing, and precipitation of soluble material with Fehling solution. The structural assignments were carried out using mono- and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses. The polysaccharides were characterized as linear, partially 3-O-methylated (1 → 6)-linked α-galactopyranans, containing only Gal and 3-O-Me-Gal, in 2:1 and 1:1 molar ratios, with molar masses of 37.6 × 10 g/mol and 28.5 × 10 g/mol, respectively. Similar structures have been described for other Pleurotus spp., but showing a lower content of 3-O-Me-Gal.
Effect of gamma irradiation on the nutritional quality of Agaricus bisporus strains cultivated in... more Effect of gamma irradiation on the nutritional quality of Agaricus bisporus strains cultivated in different composts
Universidade Estadual Paulista, UNESP, Faculdade de Ciencias Agronomicas, FCA, Departamento de Pr... more Universidade Estadual Paulista, UNESP, Faculdade de Ciencias Agronomicas, FCA, Departamento de Producao Vegetal/Defesa Fitossanitaria, Modulo de Cogumelos. Rua Jose Barbosa de Barros, 1780 - Fazenda Lageado. Caixa Postal 237, 18610-307. Botucatu, SP, Brasil
Universidade Estadual Paulista (UNESP), Faculdade de Ciencias Agronomicas (FCA), Departamento de ... more Universidade Estadual Paulista (UNESP), Faculdade de Ciencias Agronomicas (FCA), Departamento de Producao e Melhoramento Vegetal, Botucatu, SP, Brasil
Universidade Estadual Paulista (UNESP), Faculdade de Ciencias Agronomicas de Botucatu, Departamen... more Universidade Estadual Paulista (UNESP), Faculdade de Ciencias Agronomicas de Botucatu, Departamento de Ciencias Florestais, Fazenda Experimental Lageado
Universidade Estadual Paulista (UNESP), Faculdade de Ciencias Agronomicas (FCA), Departamento de ... more Universidade Estadual Paulista (UNESP), Faculdade de Ciencias Agronomicas (FCA), Departamento de Ciencia Florestal, Botucatu, SP, Brasil
The inhibition of arginase from Leishmania spp. is considered a promising approach to the leishma... more The inhibition of arginase from Leishmania spp. is considered a promising approach to the leishmaniasis treatment. In this study, the potential of a fucogalactan isolated from the medicinal mushroom Agrocybe aegerita was evaluated against arginase (ARG) from Leishmania amazonensis. The polysaccharide was obtained via aqueous extraction, and purified by freeze thawing and precipitation with Fehling solution. Its chemical structure was established by monosaccharide composition, methylation analysis, partial acid hydrolysis, and NMR spectroscopy. The data indicated that it is a fucogalactan (FG-Aa; M = 13.8 kDa), having a (1→6)-linked α-D-Galp main-chain partially substituted in O-2 by non-reducing end-units of α-L-Fucp. FG-Aa showed significant inhibitory activity on ARG with ICpotency of 5.82 ± 0.57 μM. The mechanism of ARG inhibition by the heterogalactan was the competitive type, with Kof 1.54 ± 0.15 μM. This is the first report of an inhibitory activity of arginase from L. amazone...
Pleurotus citrinopileatus, popularly known as "golden oyster mushroom" have medicinal p... more Pleurotus citrinopileatus, popularly known as "golden oyster mushroom" have medicinal properties, which are attributed mainly to the presence of bioactive polysaccharides. In this work, two partially 3-O-methylated galactans were isolated from the fruiting bodies of this fungus, via successive aqueous extraction, followed by fractionation by freeze-thawing, and precipitation of soluble material with Fehling solution. The structural assignments were carried out using mono- and bidimensional NMR spectroscopy, monosaccharide composition, and methylation analyses. The polysaccharides were characterized as linear, partially 3-O-methylated (1 → 6)-linked α-galactopyranans, containing only Gal and 3-O-Me-Gal, in 2:1 and 1:1 molar ratios, with molar masses of 37.6 × 10 g/mol and 28.5 × 10 g/mol, respectively. Similar structures have been described for other Pleurotus spp., but showing a lower content of 3-O-Me-Gal.
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