A utilização de plantas medicinais/fitoterápicos (PMF) no tratamento de doenças é uma prática mil... more A utilização de plantas medicinais/fitoterápicos (PMF) no tratamento de doenças é uma prática milenar oriunda do empirismo. Mesmo com o avanço do conhecimento científico para a produção sintética de fármacos, o uso dessas plantas se manteve inalterável e variando de acordo a economia e o desenvolvimento de alguns países ou regiões, continua sendo a principal fonte terapêutica, tendo como relato a crença de serem totalmente seguros. Ao logo do tempo, a utilização dessas plantas para tratamento de doenças/enfermidades colaborou para o surgimento de medicamentos fitoterápicos, que possui uma eficácia reconhecida para tratamento da sintomatologia ou até mesmo a cura de doenças. O uso de fitoterápicos concomitantes a medicamentos convencionais pode desencadear uma série de alterações farmacocinética nesses usuários, diminuindo a segurança e eficácia e podendo gerar uma falha terapêutica, tendo em vista que os fitoterápicos e as plantas medicinais possuem ativos. Sabe-se que muitos pacien...
A Toxicologia forense permite um conjunto de condutas acerca das intoxicações por drogas de abuso... more A Toxicologia forense permite um conjunto de condutas acerca das intoxicações por drogas de abuso como a cafeína, substância apreciada por suas ações estimulante, anorexígena e diurética, especialmente por portadores de anorexia nervosa (AN). Diferentes estudos relatam os benefícios das xantinas frente à patologias e distúrbios como a apneia do recém-nascido, depressão, doença de Alzheimer, de Parkinson, por exemplo. Em paralelo, evidenciam também a possibilidade de causar tolerância, dependência, abstinência e intoxicação. Podendo, inclusive, ser fatal para alguns indivíduos, sobretudo quando em associação a outras drogas. Diante das evidências, o objetivo deste trabalho foi o de gerar discussão, conscientização e desenvolvimento de medidas de prevenção à dependência e intoxicação, seguindo manual detalhado e padronizado de boas práticas, no que tange os cuidados com analitos. Para tanto, foi realizada uma revisão literária a fim de contextualizar o problema do uso irracional de dr...
Travel medicine and infectious disease, Jan 31, 2018
The epidemiology of extended-spectrum β-lactamase (ESBL)-producing bacteria is fast evolving with... more The epidemiology of extended-spectrum β-lactamase (ESBL)-producing bacteria is fast evolving with increasing global trend towards community-acquired infections. Limited information available about ESBLs therapy outcomes and control strategies, especially in the Middle Eastern countries. We studied 399 ESBL-producing Escherichia coli and Klebsiella pneumoniae isolates from urinary tract infections (UTIs) occurred between 2014 and 2016 in University Hospital Sharjah. We included 124 ESBL-negative E. coli and K. pneumoniae isolates from UTIs as controls. Pearson Chi squares test and independent t-test were used to compare difference between ESBL positives and negatives. Multivariate logistic regression analysis was also performed. Approximately 75% of the E. coli and K. pneumoniae isolates causing UTIs were community-acquired. We found that recurrent UTIs, old age, and catheterization among other risk factors for community-acquired ESBL-positive UTIs. Majority of these ESBL-positive is...
O envelhecimento da população tem algumas tendências que são similares a nível internacional. O a... more O envelhecimento da população tem algumas tendências que são similares a nível internacional. O aumento da população de idade avançada devido à baixa natalidade, elevado índice de esperança de vida, desenvolvimento de novas tecnologias com possibilidades de tratamentos, outrora, desconhecidos, além de, uma perspectiva de um prognóstico favorável de vida direcionados a Transtornos Associados a Doenças Neurodegenerativas (TADN). A demência, relacionada a Doença de Alzheimer (DA) se apresenta como uma das causas mais comuns de TADN. Este estudo possui caráter exploratório, na modalidade de revisão integrativa de literatura e pesquisa qualitativa, onde por meio de levantamentos bibliográficos, objetiva-se abordar a DA e seus sintomas, bem como modelos de intervenção e os impactos neurodegenerativos na qualidade de vida do indivíduo idoso. Outrossim, reputa-se compreender as divergências físicas e cognitivas no desempenho nevrológico entre neurotípicos e pessoas no contexto da Doença de ...
Many pharmaceutical chemical residues (PCR) have shown great potential to pollute the environment... more Many pharmaceutical chemical residues (PCR) have shown great potential to pollute the environment, especially in marine habitats. The conjecture stems from direct participation of human beings. Toxicological studies carried with drugs showed the possibility of appearance of physiological sexual alterations already observed in certain species of fish and other marine animals, in addition to the increase of resistant bacteria. In this way, evidence drives the reduction or interruption of exposure to CRP in the environment. The objective of this work is to propose a project to receive expired medicines and other therapeutic products, without use or leftovers from inappropriate uses by several individuals. To achieve these objectives, we will carry out the following methodologies: (i) Conduct a literature review on the subject, to consolidate the problem; (ii) present an advertising plan using social communication vehicles, such as radio, television and social networks, for information ...
Corynebacterium striatum, a common constituent of the human skin microbiome, is now considered an... more Corynebacterium striatum, a common constituent of the human skin microbiome, is now considered an emerging multidrug-resistant pathogen of immunocompromised and chronically ill patients. However, little is known about the molecular mechanisms in the transition from colonization to the multidrug-resistant (MDR) invasive phenotype in clinical isolates. This study performed a comprehensive pan-genomic analysis of C. striatum, including isolates from ‘normal skin microbiome’ and from MDR infections, to gain insights into genetic factors contributing to pathogenicity and multidrug resistance in this species. For this, three novel genome sequences were obtained from clinical isolates of C. striatum of patients from Brazil, and other 24 complete or draft C. striatum genomes were retrieved from GenBank, including the ATCC6940 isolate from the Human Microbiome Project. Analysis of C. striatum strains demonstrated the presence of an open pan-genome (α = 0.852803) containing 3,816 gene familie...
Brazilian Journal of Health and Biomedical Sciences, 2022
Nowadays, Corynebacterium striatum has been reported asetiologic agent of mild to severe hospital... more Nowadays, Corynebacterium striatum has been reported asetiologic agent of mild to severe hospital-acquired infections,including patients undergoing endotracheal intubation withfatal outcome. A continuous survey of infections due multidrug-resistant (MDR) C. striatum in South American countriesremains necessary. This retrospective and prospective studyaimed to analyze clinical-microbiological features of C. striatumclinical isolates from patients attended at a Braziliantertiary care hospital, during a nine-year period. C. striatumstrains (n=130) were isolated from infected patients attended attwenty nosocomial wards, mainly of tracheal aspirate (n=53)and blood and/or intravenous catheter (n=21/n=13). Notably,original cases of nosocomial infections due to C. striatum wereverified in children making use of invasive devices, includingone fatal neonatal case, in addition to a fatal respiratoryinfection in patient with cystic fibrosis, as well as urinarytract infections of kidney transpla...
Figure S2. Pulsed-field gel electrophoresis (PFGE) profiles of Brazilian Corynebacterium striatum... more Figure S2. Pulsed-field gel electrophoresis (PFGE) profiles of Brazilian Corynebacterium striatum isolates from blood and catheter segments. Lane 1: λ DNA ladder PFGE marker; lanes 2, 3 and 7: profile I (isolates 2089, 2091 and 2023, respectively); lane 4: profile V (isolate 2130); lanes 5, 6 and 9: profile VI (isolates 2228, 2230 and 2237, respectively); lane 8: profile VII (isolate 2296) and lane 10: profile IV (isolate 1954 – control). (TIF 469 kb)
Corynebacterium striatum commonly colonizes the normal skin and nasopharyngeal tract of humans; h... more Corynebacterium striatum commonly colonizes the normal skin and nasopharyngeal tract of humans; however, this potentially pathogenic bacterium has been identified as the causative agent of several nosocomial infections. The current study describes the draft genome of strain 1961 BR-RJ/09, isolated from the urine of a hospitalized patient from Brazil.
Figure S1. Pulsed-field gel electrophoresis (PFGE) profiles of Brazilian Corynebacterium striatum... more Figure S1. Pulsed-field gel electrophoresis (PFGE) profiles of Brazilian Corynebacterium striatum isolates from blood and catheter segments. Lane 1: λ DNA ladder PFGE marker; lane 2: profile VIII (isolate 2103); lanes 3, 5 and 6: profile I (isolates 2316, 2439 and 2023, respectively); lane 4: profile Ia (isolate 2324); lane 7: profile X (isolate 2376); lane 8: profile VIa (isolate 2390); lane 9: profile IX (isolate 2425) and lane 10: profile Ib (isolate 2454). (TIF 523 kb)
Corynebacterium striatum is a potentially pathogenic microorganism that causes nosocomial outbrea... more Corynebacterium striatum is a potentially pathogenic microorganism that causes nosocomial outbreaks. However, little is known about its virulence factors that may contribute to healthcare-associated infections (HAIs). We investigated the biofilm production on abiotic surfaces of multidrug-resistant (MDR) and multidrug-susceptible (MDS) strains of C. striatum of pulsed-field gel electrophoresis types I-MDR, II-MDR, III-MDS and IV-MDS isolated during a nosocomial outbreak in Rio de Janeiro, Brazil. The results showed that C. striatum was able to adhere to hydrophilic and hydrophobic abiotic surfaces. The C. striatum 1987/I-MDR strain, predominantly isolated from patients undergoing endotracheal intubation procedures, showed the greatest ability to adhere to all surfaces. C. striatum bound fibrinogen to its surface, which contributed to biofilm formation. Scanning electron microscopy showed the production of mature biofilms on polyurethane catheters by all pulsotypes. In conclusion, bi...
Corynebacterium striatum, a bacterium that is part of the normal skin microbiota, is also an oppo... more Corynebacterium striatum, a bacterium that is part of the normal skin microbiota, is also an opportunistic pathogen. In recent years, reports of infections and in-hospital and nosocomial outbreaks caused by antimicrobial multidrug-resistant C. striatum strains have been increasing worldwide. However, there are no studies about the genomic determinants related to antimicrobial resistance in C. striatum. This review updates global information related to antimicrobial resistance found in C. striatum and highlights the essential genomic aspects in its persistence and dissemination. The resistome of C. striatum comprises chromosomal and acquired elements. Resistance to fluoroquinolones and daptomycin are due to mutations in chromosomal genes. Conversely, resistance to macrolides, tetracyclines, phenicols, beta-lactams, and aminoglycosides are associated with mobile genomic elements such as plasmids and transposons. The presence and diversity of insertion sequences suggest an essential ro...
A utilização de plantas medicinais/fitoterápicos (PMF) no tratamento de doenças é uma prática mil... more A utilização de plantas medicinais/fitoterápicos (PMF) no tratamento de doenças é uma prática milenar oriunda do empirismo. Mesmo com o avanço do conhecimento científico para a produção sintética de fármacos, o uso dessas plantas se manteve inalterável e variando de acordo a economia e o desenvolvimento de alguns países ou regiões, continua sendo a principal fonte terapêutica, tendo como relato a crença de serem totalmente seguros. Ao logo do tempo, a utilização dessas plantas para tratamento de doenças/enfermidades colaborou para o surgimento de medicamentos fitoterápicos, que possui uma eficácia reconhecida para tratamento da sintomatologia ou até mesmo a cura de doenças. O uso de fitoterápicos concomitantes a medicamentos convencionais pode desencadear uma série de alterações farmacocinética nesses usuários, diminuindo a segurança e eficácia e podendo gerar uma falha terapêutica, tendo em vista que os fitoterápicos e as plantas medicinais possuem ativos. Sabe-se que muitos pacien...
A Toxicologia forense permite um conjunto de condutas acerca das intoxicações por drogas de abuso... more A Toxicologia forense permite um conjunto de condutas acerca das intoxicações por drogas de abuso como a cafeína, substância apreciada por suas ações estimulante, anorexígena e diurética, especialmente por portadores de anorexia nervosa (AN). Diferentes estudos relatam os benefícios das xantinas frente à patologias e distúrbios como a apneia do recém-nascido, depressão, doença de Alzheimer, de Parkinson, por exemplo. Em paralelo, evidenciam também a possibilidade de causar tolerância, dependência, abstinência e intoxicação. Podendo, inclusive, ser fatal para alguns indivíduos, sobretudo quando em associação a outras drogas. Diante das evidências, o objetivo deste trabalho foi o de gerar discussão, conscientização e desenvolvimento de medidas de prevenção à dependência e intoxicação, seguindo manual detalhado e padronizado de boas práticas, no que tange os cuidados com analitos. Para tanto, foi realizada uma revisão literária a fim de contextualizar o problema do uso irracional de dr...
Travel medicine and infectious disease, Jan 31, 2018
The epidemiology of extended-spectrum β-lactamase (ESBL)-producing bacteria is fast evolving with... more The epidemiology of extended-spectrum β-lactamase (ESBL)-producing bacteria is fast evolving with increasing global trend towards community-acquired infections. Limited information available about ESBLs therapy outcomes and control strategies, especially in the Middle Eastern countries. We studied 399 ESBL-producing Escherichia coli and Klebsiella pneumoniae isolates from urinary tract infections (UTIs) occurred between 2014 and 2016 in University Hospital Sharjah. We included 124 ESBL-negative E. coli and K. pneumoniae isolates from UTIs as controls. Pearson Chi squares test and independent t-test were used to compare difference between ESBL positives and negatives. Multivariate logistic regression analysis was also performed. Approximately 75% of the E. coli and K. pneumoniae isolates causing UTIs were community-acquired. We found that recurrent UTIs, old age, and catheterization among other risk factors for community-acquired ESBL-positive UTIs. Majority of these ESBL-positive is...
O envelhecimento da população tem algumas tendências que são similares a nível internacional. O a... more O envelhecimento da população tem algumas tendências que são similares a nível internacional. O aumento da população de idade avançada devido à baixa natalidade, elevado índice de esperança de vida, desenvolvimento de novas tecnologias com possibilidades de tratamentos, outrora, desconhecidos, além de, uma perspectiva de um prognóstico favorável de vida direcionados a Transtornos Associados a Doenças Neurodegenerativas (TADN). A demência, relacionada a Doença de Alzheimer (DA) se apresenta como uma das causas mais comuns de TADN. Este estudo possui caráter exploratório, na modalidade de revisão integrativa de literatura e pesquisa qualitativa, onde por meio de levantamentos bibliográficos, objetiva-se abordar a DA e seus sintomas, bem como modelos de intervenção e os impactos neurodegenerativos na qualidade de vida do indivíduo idoso. Outrossim, reputa-se compreender as divergências físicas e cognitivas no desempenho nevrológico entre neurotípicos e pessoas no contexto da Doença de ...
Many pharmaceutical chemical residues (PCR) have shown great potential to pollute the environment... more Many pharmaceutical chemical residues (PCR) have shown great potential to pollute the environment, especially in marine habitats. The conjecture stems from direct participation of human beings. Toxicological studies carried with drugs showed the possibility of appearance of physiological sexual alterations already observed in certain species of fish and other marine animals, in addition to the increase of resistant bacteria. In this way, evidence drives the reduction or interruption of exposure to CRP in the environment. The objective of this work is to propose a project to receive expired medicines and other therapeutic products, without use or leftovers from inappropriate uses by several individuals. To achieve these objectives, we will carry out the following methodologies: (i) Conduct a literature review on the subject, to consolidate the problem; (ii) present an advertising plan using social communication vehicles, such as radio, television and social networks, for information ...
Corynebacterium striatum, a common constituent of the human skin microbiome, is now considered an... more Corynebacterium striatum, a common constituent of the human skin microbiome, is now considered an emerging multidrug-resistant pathogen of immunocompromised and chronically ill patients. However, little is known about the molecular mechanisms in the transition from colonization to the multidrug-resistant (MDR) invasive phenotype in clinical isolates. This study performed a comprehensive pan-genomic analysis of C. striatum, including isolates from ‘normal skin microbiome’ and from MDR infections, to gain insights into genetic factors contributing to pathogenicity and multidrug resistance in this species. For this, three novel genome sequences were obtained from clinical isolates of C. striatum of patients from Brazil, and other 24 complete or draft C. striatum genomes were retrieved from GenBank, including the ATCC6940 isolate from the Human Microbiome Project. Analysis of C. striatum strains demonstrated the presence of an open pan-genome (α = 0.852803) containing 3,816 gene familie...
Brazilian Journal of Health and Biomedical Sciences, 2022
Nowadays, Corynebacterium striatum has been reported asetiologic agent of mild to severe hospital... more Nowadays, Corynebacterium striatum has been reported asetiologic agent of mild to severe hospital-acquired infections,including patients undergoing endotracheal intubation withfatal outcome. A continuous survey of infections due multidrug-resistant (MDR) C. striatum in South American countriesremains necessary. This retrospective and prospective studyaimed to analyze clinical-microbiological features of C. striatumclinical isolates from patients attended at a Braziliantertiary care hospital, during a nine-year period. C. striatumstrains (n=130) were isolated from infected patients attended attwenty nosocomial wards, mainly of tracheal aspirate (n=53)and blood and/or intravenous catheter (n=21/n=13). Notably,original cases of nosocomial infections due to C. striatum wereverified in children making use of invasive devices, includingone fatal neonatal case, in addition to a fatal respiratoryinfection in patient with cystic fibrosis, as well as urinarytract infections of kidney transpla...
Figure S2. Pulsed-field gel electrophoresis (PFGE) profiles of Brazilian Corynebacterium striatum... more Figure S2. Pulsed-field gel electrophoresis (PFGE) profiles of Brazilian Corynebacterium striatum isolates from blood and catheter segments. Lane 1: λ DNA ladder PFGE marker; lanes 2, 3 and 7: profile I (isolates 2089, 2091 and 2023, respectively); lane 4: profile V (isolate 2130); lanes 5, 6 and 9: profile VI (isolates 2228, 2230 and 2237, respectively); lane 8: profile VII (isolate 2296) and lane 10: profile IV (isolate 1954 – control). (TIF 469 kb)
Corynebacterium striatum commonly colonizes the normal skin and nasopharyngeal tract of humans; h... more Corynebacterium striatum commonly colonizes the normal skin and nasopharyngeal tract of humans; however, this potentially pathogenic bacterium has been identified as the causative agent of several nosocomial infections. The current study describes the draft genome of strain 1961 BR-RJ/09, isolated from the urine of a hospitalized patient from Brazil.
Figure S1. Pulsed-field gel electrophoresis (PFGE) profiles of Brazilian Corynebacterium striatum... more Figure S1. Pulsed-field gel electrophoresis (PFGE) profiles of Brazilian Corynebacterium striatum isolates from blood and catheter segments. Lane 1: λ DNA ladder PFGE marker; lane 2: profile VIII (isolate 2103); lanes 3, 5 and 6: profile I (isolates 2316, 2439 and 2023, respectively); lane 4: profile Ia (isolate 2324); lane 7: profile X (isolate 2376); lane 8: profile VIa (isolate 2390); lane 9: profile IX (isolate 2425) and lane 10: profile Ib (isolate 2454). (TIF 523 kb)
Corynebacterium striatum is a potentially pathogenic microorganism that causes nosocomial outbrea... more Corynebacterium striatum is a potentially pathogenic microorganism that causes nosocomial outbreaks. However, little is known about its virulence factors that may contribute to healthcare-associated infections (HAIs). We investigated the biofilm production on abiotic surfaces of multidrug-resistant (MDR) and multidrug-susceptible (MDS) strains of C. striatum of pulsed-field gel electrophoresis types I-MDR, II-MDR, III-MDS and IV-MDS isolated during a nosocomial outbreak in Rio de Janeiro, Brazil. The results showed that C. striatum was able to adhere to hydrophilic and hydrophobic abiotic surfaces. The C. striatum 1987/I-MDR strain, predominantly isolated from patients undergoing endotracheal intubation procedures, showed the greatest ability to adhere to all surfaces. C. striatum bound fibrinogen to its surface, which contributed to biofilm formation. Scanning electron microscopy showed the production of mature biofilms on polyurethane catheters by all pulsotypes. In conclusion, bi...
Corynebacterium striatum, a bacterium that is part of the normal skin microbiota, is also an oppo... more Corynebacterium striatum, a bacterium that is part of the normal skin microbiota, is also an opportunistic pathogen. In recent years, reports of infections and in-hospital and nosocomial outbreaks caused by antimicrobial multidrug-resistant C. striatum strains have been increasing worldwide. However, there are no studies about the genomic determinants related to antimicrobial resistance in C. striatum. This review updates global information related to antimicrobial resistance found in C. striatum and highlights the essential genomic aspects in its persistence and dissemination. The resistome of C. striatum comprises chromosomal and acquired elements. Resistance to fluoroquinolones and daptomycin are due to mutations in chromosomal genes. Conversely, resistance to macrolides, tetracyclines, phenicols, beta-lactams, and aminoglycosides are associated with mobile genomic elements such as plasmids and transposons. The presence and diversity of insertion sequences suggest an essential ro...
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