STRESS-RELATED GENES IN WOODY PLANTS: TRANSCRIPTIONAL AND POST-TRANSCRIPTIONAL REGULATION J. CAIR... more STRESS-RELATED GENES IN WOODY PLANTS: TRANSCRIPTIONAL AND POST-TRANSCRIPTIONAL REGULATION J. CAIRNEY, DK VILLALON, S. CHANG, Institute of ... Our library screening did not select any of the Lea family of genes, often isolated by such procedures ...
Eucalyptus species are among the fastest growing woody plants in the world and represent about 8 ... more Eucalyptus species are among the fastest growing woody plants in the world and represent about 8 % of all planted forests (~18 million hectares) grown in 90 countries [1]. Only a limited number of species are grown commercially, and these have been the focus of extensive breeding to improve desirable wood properties such as basic density, cellulose content, fiber length and improved growth. Improved Eucalyptus varieties grown in managed plantations as a source of timber and pulpwood have provided socio-economic benefits for both small and large land owners. Eucalyptus also provides a cost-effective source of lignocelluloses for the production of energy and advanced biofuels. However, the large scale plantings of the most productive Eucalyptus species in the Southeast U.S. are currently limited to regions of central and southern Florida where no hard freezes occur in winter. Plantings of hardwood species to support the traditional forest industry in the Southeastern U.S. are infreque...
SummaryCowpea is one of the most important legume crops planted worldwide, especially in Sub-Saha... more SummaryCowpea is one of the most important legume crops planted worldwide, especially in Sub-Saharan Africa and Asia. Despite decades of effort, genetic engineering of cowpea is still challenging due to inefficient in vitro shoot regeneration, Agrobacterium-mediated T-DNA delivery and transgenic selection. Here, we report a rapid and highly efficient cowpea transformation system using embryonic axis explants isolated from imbibed mature seeds. We found that removal of the shoot apical meristem by cutting through the middle of the epicotyl stimulated direct multiple shoot organogenesis from the cotyledonary node tissue. Furthermore, the application of a ternary transformation vector system using an optimized pVIR accessory plasmid provided high levels of Agrobacterium-mediated gene delivery. The utilization of spectinomycin as the selection agent enabled more efficient transgenic selection and plant recovery. Transgenic cowpea shoots developed exclusively from the cotyledonary nodes ...
La presente invention se rapporte a un procede independant du genotype permettant de transformer ... more La presente invention se rapporte a un procede independant du genotype permettant de transformer et de selectionner des explants vegetaux. Le procede de transformation consiste a cultiver prealablement les explants en presence d'un inducteur Agrobacterium, et a mettre les explants transformes en contact avec un milieu de regeneration de pousses qui accelere le developpement des pousses. L'invention concerne egalement des plantes regenerees a l'aide dudit procede de transformation. En particulier, l'invention se rapporte a des procedes permettant d'obtenir des cellules d'Eucalyptus et de pins transgeniques, et de regenerer des Eucalyptus et des pins transformes de maniere stable. L'invention concerne aussi des milieux, des procedes et des plasmides permettant de selectionner et de regenerer des plantes, en particulier des arbres forestiers.
The productivity of plantation forests is essential to meet the future world demand for wood and ... more The productivity of plantation forests is essential to meet the future world demand for wood and wood products in a sustainable fashion and in a manner that preserves natural stands and biodiversity. Plantation forestry has enormously benefited from development and implementation of improved silvicultural and forest management practices during the past century. A second wave of improvements has been brought about by the introduction of new germplasm developed through genetics and breeding efforts for both hardwood and conifer tree species. Coupled with the genetic gains achieved through tree breeding, the emergence of new biotechnological approaches that span the fields of plant developmental biology, genetic transformation, and discovery of genes associated with complex multigenic traits have added a new dimension to forest tree improvement programs. Significant progress has been made during the past five years in the area of plant regeneration via organogenesis and somatic embryog...
SUMMARY Cowpea (Vigna unguiculata (L.) Walp.) is one of the most important legume crops planted w... more SUMMARY Cowpea (Vigna unguiculata (L.) Walp.) is one of the most important legume crops planted worldwide, but despite decades of effort, cowpea transformation is still challenging due to inefficient Agrobacterium‐mediated transfer DNA delivery, transgenic selection and in vitro shoot regeneration. Here, we report a highly efficient transformation system using embryonic axis explants isolated from imbibed mature seeds. We found that removal of the shoot apical meristem from the explants stimulated direct multiple shoot organogenesis from the cotyledonary node tissue. The application of a previously reported ternary transformation vector system provided efficient Agrobacterium‐mediated gene delivery, while the utilization of spcN as selectable marker enabled more robust transgenic selection, plant recovery and transgenic plant generation without escapes and chimera formation. Transgenic cowpea plantlets developed exclusively from the cotyledonary nodes at frequencies of 4% to 37% across a wide range of cowpea genotypes. CRISPR/Cas‐mediated gene editing was successfully demonstrated. The transformation principles established here could also be applied to other legumes to increase transformation efficiencies.
Page 1. PHYSIOLOGIA PLANTARUM 97: 139-148, 1996 Co Printed in Denmark - all rights reserved [ss^ ... more Page 1. PHYSIOLOGIA PLANTARUM 97: 139-148, 1996 Co Printed in Denmark - all rights reserved [ss^ 003] -93! 7 Gene expression under water deiicit in loblolly pine (Pinus taeda): Isolation and characterization of cDNA clones ...
STRESS-RELATED GENES IN WOODY PLANTS: TRANSCRIPTIONAL AND POST-TRANSCRIPTIONAL REGULATION J. CAIR... more STRESS-RELATED GENES IN WOODY PLANTS: TRANSCRIPTIONAL AND POST-TRANSCRIPTIONAL REGULATION J. CAIRNEY, DK VILLALON, S. CHANG, Institute of ... Our library screening did not select any of the Lea family of genes, often isolated by such procedures ...
Eucalyptus species are among the fastest growing woody plants in the world and represent about 8 ... more Eucalyptus species are among the fastest growing woody plants in the world and represent about 8 % of all planted forests (~18 million hectares) grown in 90 countries [1]. Only a limited number of species are grown commercially, and these have been the focus of extensive breeding to improve desirable wood properties such as basic density, cellulose content, fiber length and improved growth. Improved Eucalyptus varieties grown in managed plantations as a source of timber and pulpwood have provided socio-economic benefits for both small and large land owners. Eucalyptus also provides a cost-effective source of lignocelluloses for the production of energy and advanced biofuels. However, the large scale plantings of the most productive Eucalyptus species in the Southeast U.S. are currently limited to regions of central and southern Florida where no hard freezes occur in winter. Plantings of hardwood species to support the traditional forest industry in the Southeastern U.S. are infreque...
SummaryCowpea is one of the most important legume crops planted worldwide, especially in Sub-Saha... more SummaryCowpea is one of the most important legume crops planted worldwide, especially in Sub-Saharan Africa and Asia. Despite decades of effort, genetic engineering of cowpea is still challenging due to inefficient in vitro shoot regeneration, Agrobacterium-mediated T-DNA delivery and transgenic selection. Here, we report a rapid and highly efficient cowpea transformation system using embryonic axis explants isolated from imbibed mature seeds. We found that removal of the shoot apical meristem by cutting through the middle of the epicotyl stimulated direct multiple shoot organogenesis from the cotyledonary node tissue. Furthermore, the application of a ternary transformation vector system using an optimized pVIR accessory plasmid provided high levels of Agrobacterium-mediated gene delivery. The utilization of spectinomycin as the selection agent enabled more efficient transgenic selection and plant recovery. Transgenic cowpea shoots developed exclusively from the cotyledonary nodes ...
La presente invention se rapporte a un procede independant du genotype permettant de transformer ... more La presente invention se rapporte a un procede independant du genotype permettant de transformer et de selectionner des explants vegetaux. Le procede de transformation consiste a cultiver prealablement les explants en presence d'un inducteur Agrobacterium, et a mettre les explants transformes en contact avec un milieu de regeneration de pousses qui accelere le developpement des pousses. L'invention concerne egalement des plantes regenerees a l'aide dudit procede de transformation. En particulier, l'invention se rapporte a des procedes permettant d'obtenir des cellules d'Eucalyptus et de pins transgeniques, et de regenerer des Eucalyptus et des pins transformes de maniere stable. L'invention concerne aussi des milieux, des procedes et des plasmides permettant de selectionner et de regenerer des plantes, en particulier des arbres forestiers.
The productivity of plantation forests is essential to meet the future world demand for wood and ... more The productivity of plantation forests is essential to meet the future world demand for wood and wood products in a sustainable fashion and in a manner that preserves natural stands and biodiversity. Plantation forestry has enormously benefited from development and implementation of improved silvicultural and forest management practices during the past century. A second wave of improvements has been brought about by the introduction of new germplasm developed through genetics and breeding efforts for both hardwood and conifer tree species. Coupled with the genetic gains achieved through tree breeding, the emergence of new biotechnological approaches that span the fields of plant developmental biology, genetic transformation, and discovery of genes associated with complex multigenic traits have added a new dimension to forest tree improvement programs. Significant progress has been made during the past five years in the area of plant regeneration via organogenesis and somatic embryog...
SUMMARY Cowpea (Vigna unguiculata (L.) Walp.) is one of the most important legume crops planted w... more SUMMARY Cowpea (Vigna unguiculata (L.) Walp.) is one of the most important legume crops planted worldwide, but despite decades of effort, cowpea transformation is still challenging due to inefficient Agrobacterium‐mediated transfer DNA delivery, transgenic selection and in vitro shoot regeneration. Here, we report a highly efficient transformation system using embryonic axis explants isolated from imbibed mature seeds. We found that removal of the shoot apical meristem from the explants stimulated direct multiple shoot organogenesis from the cotyledonary node tissue. The application of a previously reported ternary transformation vector system provided efficient Agrobacterium‐mediated gene delivery, while the utilization of spcN as selectable marker enabled more robust transgenic selection, plant recovery and transgenic plant generation without escapes and chimera formation. Transgenic cowpea plantlets developed exclusively from the cotyledonary nodes at frequencies of 4% to 37% across a wide range of cowpea genotypes. CRISPR/Cas‐mediated gene editing was successfully demonstrated. The transformation principles established here could also be applied to other legumes to increase transformation efficiencies.
Page 1. PHYSIOLOGIA PLANTARUM 97: 139-148, 1996 Co Printed in Denmark - all rights reserved [ss^ ... more Page 1. PHYSIOLOGIA PLANTARUM 97: 139-148, 1996 Co Printed in Denmark - all rights reserved [ss^ 003] -93! 7 Gene expression under water deiicit in loblolly pine (Pinus taeda): Isolation and characterization of cDNA clones ...
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