The aim of this study was to explore the genetic polymorphisms in LTF/EcoRI and TLR4/AluI loci an... more The aim of this study was to explore the genetic polymorphisms in LTF/EcoRI and TLR4/AluI loci and their association with milk and reproductive performance in Holstein cattle. A randomly selected 800 Holstein dairy cows from two dairy farms (400 animals each) in Egypt were used. Based on the two farm records, association between LTF/EcoRI genotypes and milk performance traits (order of lactation, daily milk yield, days in milk, corrected milk at 305 day and dry period) was carried out. Meanwhile, exploring of TLR4/AluI genotypes effect was done on data for reproductive performance (age at first freshening, calving interval, number of services per conception, ovarian rebound and days open). DNA was extracted from blood samples collected from Holstein dairy cows of the both farms and restriction analysis of 301-bp PCR products of LTF gene revealed two genotypes: AA genotype (301 bp) and AB genotype (301, 201 and 100 bp). Meanwhile, restriction analysis of 382-bp PCR products of TLR4 gene digested with AluI yielded two alleles (A and B) and three genotypes (AA, AB and BB). The A allele was indicated by two bands at 300 and 82 bp, and the B allele resulted in three fragments of 160, 140 and 82 bp. There was a significant association (p ≤ 0.05) between LTF genotypes and milk performance traits except for days in milk. The TLR4 genotypes had significant effects (p ≤ 0.05) on age at first freshening, calving interval, number of services per conception, ovarian rebound and days open. Ordinal logistic regression statistical model also revealed that it is possible to calculate high reproductive performance traits and to predict favourable dairy cows based on LTF and TLR4 genotypes. This research reveals the effectiveness of LTF/EcoRI and TLR4/AluI loci as candidates for reproductive performance assessment in Holstein cattle.
Abstract: Genetic variability among four Egyptian sheep breeds (Barki, Rahmani, Ossimi and Romano... more Abstract: Genetic variability among four Egyptian sheep breeds (Barki, Rahmani, Ossimi and Romanov) were studied using both random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) techniques. Sixteen random primers were used to amplify DNA fragments in these four sheep breeds. RAPD patterns with a level of polymorphism were detected among breeds. The results showed that the genetic similarity among these four breeds was as follows: 90% (Barki x Ossimi), 87% (Barki x Rahmani), 87% (Ossimi x Rahmani), 80% (Ossimi x Romanov), 80% (Rahmani x Romanov) and 74% (Barki x Romanov). However, the closer proximity or the highest genetic similarity was observed between Barki and Ossimi (90%), while the lowest was observed between Barki and Romanov (74%). On the other hand, the results of the RFLP for 18S rRNA gene technique showed that, no genetic variation were found among the four sheep breeds under study. Finding in this study confirms the phenotypic classi...
Salah Abdel-Rahman1*, Abeer El-Nahas2, Shaaban Hemeda2, Sherif Nasr2 1Department of NucleicAcid R... more Salah Abdel-Rahman1*, Abeer El-Nahas2, Shaaban Hemeda2, Sherif Nasr2 1Department of NucleicAcid Research, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and TechnologicalApplications,Alexandria, (EGYPT) 2Department ofAnimal Husbandry and Wealth Development, Faculty of Veterinary Medicine, Alexandria University, (EGYPT) E-mail: salahmaa@yahoo.com Received: 13th October, 2013 ; Accepted: 16th November, 2013
Iranian Journal of Pharmaceutical Research : IJPR, 2020
Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is a ubiquitous multifunctional ... more Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is a ubiquitous multifunctional protein required in the DNA base excision repair pathway and a noteworthy reducing-oxidizing factor that regulates the activity of various transcription factors. Cyclin-dependent kinases (CDKs) assume a key role in directing the progression of the cell- cycle. The present study evaluated the synergistic efficacy of using licochalcone B (LCB) and fullerene C60 (FnC60) nanoparticles against diethylnitrosamine (DEN)-induced hepatocarcinoma in rats and relevant signaling pathways, with APE1/Ref-1 and CDK-4, as novel anti-cancer- targeting. LCB alone and in combination with FnC60 significantly decreased DNA fragmentation, oxidative DNA damage (8-hydroxy-2′-deoxyguanosine levels), APE1/Ref-1, CDK-4, retinoblastoma, B- cell lymphoma-2 (Bcl-2), B-cell lymphoma-xL (Bcl-xL), and β-arrestin-2 mRNA expression, and APE1/Ref-1 and CDK-4 protein expression. In contrast, these treatments significantly i...
Carica papaya is a perennial plant containing bioactive constituents with free radical-scavenging... more Carica papaya is a perennial plant containing bioactive constituents with free radical-scavenging and immune-modulating activities. In contrast, the immune suppression is predominant in the periparturient period, where oxidative stress has a substantial impact on the mammary gland health. The aim of the experiment reported here was to determine the potential effect of C. papaya aqueous extract (CPE) on milk production traits, and expression of genes and proteins related to immune and antioxidant status in dairy milk somatic cells (MSCs). Forty Friesian dairy cows were divided equally between a control and CPE-treated groups (orally drenched 250 µg/kg bwt, once weekly a month before expected parturition and continued until 5 months post-partum). CPE did not affect milk yield or composition but upregulated the expression of β13-defensin (DEFB13), cathelicidin 2 (CATHL2), cathelicidin antimicrobial peptide (CATHL3), hepcidin (HAMP), lysozyme (LYZ), catalase (CAT) and glutathione peroxidase (GSH-Px) in MSCs. The environmental micro-organisms did not influence the levels of the transcripts. The DEFB13, CATHL2, CATHL3, HAMP and LYZ, but not β1-defensin (DEFB1) transcripts and proteins were constitutively expressed in MSCs obtained from pathogen-free udders. It could be concluded that CPE has immunostimulant and antioxidant activities; thereby, it could be utilized to minimize the occurrence of mastitis.
The influence of tramadol (TD) on hepatic tissue and the potential efficiency of lycopene to miti... more The influence of tramadol (TD) on hepatic tissue and the potential efficiency of lycopene to mitigate TD-induced hepatotoxic impacts were determined. Forty male albino rats were allocated into four groups: group I, untreated (placebo); group II, injected with TD (15 mg kg−1) intraperitoneally (i.p.); group III, gastrogavaged with lycopene (10 mg kg−1) per os (p.o.); and group IV received TD with lycopene with the same mentioned doses for 15 days. The results demonstrated that TD induced augmentation in tissue lipid peroxidation biomarker and disturbance in the antioxidant homeostasis and elevated the activity of serum liver injury biomarkers and decreased serum protein, globulin, and albumin. Hepatic glutathione S-transferase (GST), superoxide dismutase (SOD), thioredoxin-1 (Txn-1), and catalase (CAT) activities and gene expression were decreased and glutathione content was reduced in the TD-challenged rats, and these effects were alleviated by lycopene. Furthermore, TD induced apoptosis in liver tissues as shown by DNA fragmentation and upregulation of proapoptotic Bax and Casp-3 while lycopene upregulated the antiapoptotic Bcl-2. The results of Western blot showed that lycopene initiated low expression of mitogen activated protein kinase pathway (MAPK) protein expression in liver tissues of TD-challenged rats. In addition, lycopene reduced fatty degeneration and necrosis of the liver in TD-challenged group. Our data demonstrate that lycopene appears to be highly efficient in mitigating the hepatotoxic impacts of TD by preventing lipid peroxidation and initiating modifications in the expression and activity of antioxidant pathways. Surprisingly, lycopene fortified liver tissue by inhibiting DNA fragmentation and apoptosis signaling induced by TD. MAPK activation may be dependent from ROS generation; due to lycopene which possessed antioxidant potential did have a substantial effect on MAPK activity.
Hepatic cancer (HCC) is a well-identified dilemma throughout the world, and hence, the molecular ... more Hepatic cancer (HCC) is a well-identified dilemma throughout the world, and hence, the molecular mechanisms and strategy for preventive protection against this malignancy are critical. S-adenosylmethionine (SAM) is a unique methyl granter in vast reactions, including DNA methylation, and secures the genome against hypomethylation, which is a hallmark of tumors. Consequently, SAM may control the rate of gene expression. The objective of this investigation was to evaluate the expression of long noncoding RNAs (lncRNAs) transcript involved in hepatic tumorigenesis, including additional coding CEBPA (ecCEBPA) and urothelial carcinoma related 1 (UCA1), antioxidant enzymes transcripts, and relevant signaling pathway in diethylnitrosamine (DEN)-prompted HCC along with their conceivable targeting by SAM at different stages of HCC in rats. Our outcomes revealed that SAM particularly when given at the starting phase downregulates ecCEBPA and UCA1 gene transcripts and ameliorate histopathological alterations in DEN-initiated HCC. Interestingly, SAM attenuates DEN-induced upregulation of PI3K/Akt protein expression. However, SAM upregulates the antioxidant enzymes mRNA transcripts and effectively diminishing DNA oxidation. The results of a DNA fragmentation assay further support the capacity of SAM to ameliorate DEN-induced hepatic malignancy. These results revealed the role of ecCEBPA and UCA1 in HCC and suggest that these lncRNAs may be helpful as prognostic and analytical biomarkers of HCC. Curiously, SAM readily targets the studied genes via inhibiting PI3K/Akt signaling pathway, which should make SAM an appealing agent for both chemoprevention and treatment of HCC.
The study aimed to verify parentage and genetic variability among twelve Arabian horses using fiv... more The study aimed to verify parentage and genetic variability among twelve Arabian horses using five ISSR markers. Forty-five DNA loci, which extracted for the sire, mare and foal were amplified and replicated using the tested primers with an average of 9.0 loci per primer, and every marker is compared. The total number of polymorphic alleles was 33 (73.33% polymorphism), with an average of 6.6 alleles per primer. The markers (GAG)6G-ISSR (12 loci), (AGC)6C-ISSR (11 loci) and (AGC)6G-ISSR (9 loci) amplified the highest number of loci and possessed the highest PIC values indicating that they are the most promising for analysis of genetic variety and paternity in the Arabian horse. The highest similarity and shortest genetic distance (Si=0.926, Gd=0.077) were between Abeer and her daughter Karma. While, the lowest similarity and longest genetic distance (Si=0.353, Gd=1.041) between Sary and Farhan. The UPGMA cluster analysis supported the genetic relatedness as expected among the 12 Arabian horses. The dendrogram grouped the 12 Arabian horses according to their paternity, i.e. pedigree levels, into three main clusters within a branched-off 0.439 genetic similarity.
Spirulina platensis (SP) is a microalga with antioxidant, antidiabetic and anti-inflammatory prop... more Spirulina platensis (SP) is a microalga with antioxidant, antidiabetic and anti-inflammatory properties. The present study explored the ability and potential mechanism(s) by which SP induced glucose lowering impact in diabetic rat model. Forty rats were allocated into four groups: control; streptozotocin (STZ)-induced diabetes (STZ, 45mg/kg b.w., intraperitoneally); SP (500mg/kg b.w., orally twice weekly for 2 months) and STZ-induced diabetes+SP group. In the STZ-induced diabetic rats, SP significantly decreased (P>0.05) serum glucose, glycated hemoglobin (HbA1c), malondialdehyde (MDA) levels and significantly increased (P>0.05) serum insulin, the activity of antioxidant enzymes and normalized their mRNA gene expression. Furthermore, SP attenuates STZ-induced upregulation of the gluconeogenic enzyme pyruvate carboxylase (PC), the pro-apoptotic Bax and caspase-3 (CASP-3), tumor necrosis factor alpha (TNF-α) gene expression. The Western blot results revealed that, SP induced dow...
Canadian journal of physiology and pharmacology, 2016
The effect of monosodium glutamate (MSG) on brain tissue and the relative ability of lycopene to ... more The effect of monosodium glutamate (MSG) on brain tissue and the relative ability of lycopene to avert these neurotoxic effects were investigated. Thirty-two male Wistar rats were distributed into 4 groups: group I, untreated (placebo); group II, injected with MSG (5 mg·kg(-1)) s.c.; group III, gastrogavaged with lycopene (10 mg·kg(-1)) p.o.; and group IV received MSG with lycopene with the same mentioned doses for 30 days. The results showed that MSG induced elevation in lipid peroxidation marker and perturbation in the antioxidant homeostasis and increased the levels of brain and serum cholinesterase (ChE), total creatine phosphokinase (CPK), creatine phosphokinase isoenzymes BB (CPK-BB), and lactate dehydrogenase (LDH). Glutathione S-transferase (GST), superoxide dismutase (SOD), and catalase (CAT) activities and gene expression were increased and glutathione content was reduced in the MSG-challenged rats, and these effects were ameliorated by lycopene. Furthermore, MSG induced a...
Corresponding Author: Salah M. Abdel-Rahman, Genetic Engineering and Biotechnology Research Insti... more Corresponding Author: Salah M. Abdel-Rahman, Genetic Engineering and Biotechnology Research Institute (GEBRI), Mubarak City for Scientific Research and Technology Applications, Alexandria, Egypt. Tel.: 002 0112727468, Fax: 002 034593423 E-mail: salahmaa@yahoo. ...
The aim of this study was to explore the genetic polymorphisms in LTF/EcoRI and TLR4/AluI loci an... more The aim of this study was to explore the genetic polymorphisms in LTF/EcoRI and TLR4/AluI loci and their association with milk and reproductive performance in Holstein cattle. A randomly selected 800 Holstein dairy cows from two dairy farms (400 animals each) in Egypt were used. Based on the two farm records, association between LTF/EcoRI genotypes and milk performance traits (order of lactation, daily milk yield, days in milk, corrected milk at 305 day and dry period) was carried out. Meanwhile, exploring of TLR4/AluI genotypes effect was done on data for reproductive performance (age at first freshening, calving interval, number of services per conception, ovarian rebound and days open). DNA was extracted from blood samples collected from Holstein dairy cows of the both farms and restriction analysis of 301-bp PCR products of LTF gene revealed two genotypes: AA genotype (301 bp) and AB genotype (301, 201 and 100 bp). Meanwhile, restriction analysis of 382-bp PCR products of TLR4 gene digested with AluI yielded two alleles (A and B) and three genotypes (AA, AB and BB). The A allele was indicated by two bands at 300 and 82 bp, and the B allele resulted in three fragments of 160, 140 and 82 bp. There was a significant association (p ≤ 0.05) between LTF genotypes and milk performance traits except for days in milk. The TLR4 genotypes had significant effects (p ≤ 0.05) on age at first freshening, calving interval, number of services per conception, ovarian rebound and days open. Ordinal logistic regression statistical model also revealed that it is possible to calculate high reproductive performance traits and to predict favourable dairy cows based on LTF and TLR4 genotypes. This research reveals the effectiveness of LTF/EcoRI and TLR4/AluI loci as candidates for reproductive performance assessment in Holstein cattle.
Abstract: Genetic variability among four Egyptian sheep breeds (Barki, Rahmani, Ossimi and Romano... more Abstract: Genetic variability among four Egyptian sheep breeds (Barki, Rahmani, Ossimi and Romanov) were studied using both random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) techniques. Sixteen random primers were used to amplify DNA fragments in these four sheep breeds. RAPD patterns with a level of polymorphism were detected among breeds. The results showed that the genetic similarity among these four breeds was as follows: 90% (Barki x Ossimi), 87% (Barki x Rahmani), 87% (Ossimi x Rahmani), 80% (Ossimi x Romanov), 80% (Rahmani x Romanov) and 74% (Barki x Romanov). However, the closer proximity or the highest genetic similarity was observed between Barki and Ossimi (90%), while the lowest was observed between Barki and Romanov (74%). On the other hand, the results of the RFLP for 18S rRNA gene technique showed that, no genetic variation were found among the four sheep breeds under study. Finding in this study confirms the phenotypic classi...
Salah Abdel-Rahman1*, Abeer El-Nahas2, Shaaban Hemeda2, Sherif Nasr2 1Department of NucleicAcid R... more Salah Abdel-Rahman1*, Abeer El-Nahas2, Shaaban Hemeda2, Sherif Nasr2 1Department of NucleicAcid Research, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and TechnologicalApplications,Alexandria, (EGYPT) 2Department ofAnimal Husbandry and Wealth Development, Faculty of Veterinary Medicine, Alexandria University, (EGYPT) E-mail: salahmaa@yahoo.com Received: 13th October, 2013 ; Accepted: 16th November, 2013
Iranian Journal of Pharmaceutical Research : IJPR, 2020
Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is a ubiquitous multifunctional ... more Apurinic/apyrimidinic endonuclease 1/redox factor-1 (APE1/Ref-1) is a ubiquitous multifunctional protein required in the DNA base excision repair pathway and a noteworthy reducing-oxidizing factor that regulates the activity of various transcription factors. Cyclin-dependent kinases (CDKs) assume a key role in directing the progression of the cell- cycle. The present study evaluated the synergistic efficacy of using licochalcone B (LCB) and fullerene C60 (FnC60) nanoparticles against diethylnitrosamine (DEN)-induced hepatocarcinoma in rats and relevant signaling pathways, with APE1/Ref-1 and CDK-4, as novel anti-cancer- targeting. LCB alone and in combination with FnC60 significantly decreased DNA fragmentation, oxidative DNA damage (8-hydroxy-2′-deoxyguanosine levels), APE1/Ref-1, CDK-4, retinoblastoma, B- cell lymphoma-2 (Bcl-2), B-cell lymphoma-xL (Bcl-xL), and β-arrestin-2 mRNA expression, and APE1/Ref-1 and CDK-4 protein expression. In contrast, these treatments significantly i...
Carica papaya is a perennial plant containing bioactive constituents with free radical-scavenging... more Carica papaya is a perennial plant containing bioactive constituents with free radical-scavenging and immune-modulating activities. In contrast, the immune suppression is predominant in the periparturient period, where oxidative stress has a substantial impact on the mammary gland health. The aim of the experiment reported here was to determine the potential effect of C. papaya aqueous extract (CPE) on milk production traits, and expression of genes and proteins related to immune and antioxidant status in dairy milk somatic cells (MSCs). Forty Friesian dairy cows were divided equally between a control and CPE-treated groups (orally drenched 250 µg/kg bwt, once weekly a month before expected parturition and continued until 5 months post-partum). CPE did not affect milk yield or composition but upregulated the expression of β13-defensin (DEFB13), cathelicidin 2 (CATHL2), cathelicidin antimicrobial peptide (CATHL3), hepcidin (HAMP), lysozyme (LYZ), catalase (CAT) and glutathione peroxidase (GSH-Px) in MSCs. The environmental micro-organisms did not influence the levels of the transcripts. The DEFB13, CATHL2, CATHL3, HAMP and LYZ, but not β1-defensin (DEFB1) transcripts and proteins were constitutively expressed in MSCs obtained from pathogen-free udders. It could be concluded that CPE has immunostimulant and antioxidant activities; thereby, it could be utilized to minimize the occurrence of mastitis.
The influence of tramadol (TD) on hepatic tissue and the potential efficiency of lycopene to miti... more The influence of tramadol (TD) on hepatic tissue and the potential efficiency of lycopene to mitigate TD-induced hepatotoxic impacts were determined. Forty male albino rats were allocated into four groups: group I, untreated (placebo); group II, injected with TD (15 mg kg−1) intraperitoneally (i.p.); group III, gastrogavaged with lycopene (10 mg kg−1) per os (p.o.); and group IV received TD with lycopene with the same mentioned doses for 15 days. The results demonstrated that TD induced augmentation in tissue lipid peroxidation biomarker and disturbance in the antioxidant homeostasis and elevated the activity of serum liver injury biomarkers and decreased serum protein, globulin, and albumin. Hepatic glutathione S-transferase (GST), superoxide dismutase (SOD), thioredoxin-1 (Txn-1), and catalase (CAT) activities and gene expression were decreased and glutathione content was reduced in the TD-challenged rats, and these effects were alleviated by lycopene. Furthermore, TD induced apoptosis in liver tissues as shown by DNA fragmentation and upregulation of proapoptotic Bax and Casp-3 while lycopene upregulated the antiapoptotic Bcl-2. The results of Western blot showed that lycopene initiated low expression of mitogen activated protein kinase pathway (MAPK) protein expression in liver tissues of TD-challenged rats. In addition, lycopene reduced fatty degeneration and necrosis of the liver in TD-challenged group. Our data demonstrate that lycopene appears to be highly efficient in mitigating the hepatotoxic impacts of TD by preventing lipid peroxidation and initiating modifications in the expression and activity of antioxidant pathways. Surprisingly, lycopene fortified liver tissue by inhibiting DNA fragmentation and apoptosis signaling induced by TD. MAPK activation may be dependent from ROS generation; due to lycopene which possessed antioxidant potential did have a substantial effect on MAPK activity.
Hepatic cancer (HCC) is a well-identified dilemma throughout the world, and hence, the molecular ... more Hepatic cancer (HCC) is a well-identified dilemma throughout the world, and hence, the molecular mechanisms and strategy for preventive protection against this malignancy are critical. S-adenosylmethionine (SAM) is a unique methyl granter in vast reactions, including DNA methylation, and secures the genome against hypomethylation, which is a hallmark of tumors. Consequently, SAM may control the rate of gene expression. The objective of this investigation was to evaluate the expression of long noncoding RNAs (lncRNAs) transcript involved in hepatic tumorigenesis, including additional coding CEBPA (ecCEBPA) and urothelial carcinoma related 1 (UCA1), antioxidant enzymes transcripts, and relevant signaling pathway in diethylnitrosamine (DEN)-prompted HCC along with their conceivable targeting by SAM at different stages of HCC in rats. Our outcomes revealed that SAM particularly when given at the starting phase downregulates ecCEBPA and UCA1 gene transcripts and ameliorate histopathological alterations in DEN-initiated HCC. Interestingly, SAM attenuates DEN-induced upregulation of PI3K/Akt protein expression. However, SAM upregulates the antioxidant enzymes mRNA transcripts and effectively diminishing DNA oxidation. The results of a DNA fragmentation assay further support the capacity of SAM to ameliorate DEN-induced hepatic malignancy. These results revealed the role of ecCEBPA and UCA1 in HCC and suggest that these lncRNAs may be helpful as prognostic and analytical biomarkers of HCC. Curiously, SAM readily targets the studied genes via inhibiting PI3K/Akt signaling pathway, which should make SAM an appealing agent for both chemoprevention and treatment of HCC.
The study aimed to verify parentage and genetic variability among twelve Arabian horses using fiv... more The study aimed to verify parentage and genetic variability among twelve Arabian horses using five ISSR markers. Forty-five DNA loci, which extracted for the sire, mare and foal were amplified and replicated using the tested primers with an average of 9.0 loci per primer, and every marker is compared. The total number of polymorphic alleles was 33 (73.33% polymorphism), with an average of 6.6 alleles per primer. The markers (GAG)6G-ISSR (12 loci), (AGC)6C-ISSR (11 loci) and (AGC)6G-ISSR (9 loci) amplified the highest number of loci and possessed the highest PIC values indicating that they are the most promising for analysis of genetic variety and paternity in the Arabian horse. The highest similarity and shortest genetic distance (Si=0.926, Gd=0.077) were between Abeer and her daughter Karma. While, the lowest similarity and longest genetic distance (Si=0.353, Gd=1.041) between Sary and Farhan. The UPGMA cluster analysis supported the genetic relatedness as expected among the 12 Arabian horses. The dendrogram grouped the 12 Arabian horses according to their paternity, i.e. pedigree levels, into three main clusters within a branched-off 0.439 genetic similarity.
Spirulina platensis (SP) is a microalga with antioxidant, antidiabetic and anti-inflammatory prop... more Spirulina platensis (SP) is a microalga with antioxidant, antidiabetic and anti-inflammatory properties. The present study explored the ability and potential mechanism(s) by which SP induced glucose lowering impact in diabetic rat model. Forty rats were allocated into four groups: control; streptozotocin (STZ)-induced diabetes (STZ, 45mg/kg b.w., intraperitoneally); SP (500mg/kg b.w., orally twice weekly for 2 months) and STZ-induced diabetes+SP group. In the STZ-induced diabetic rats, SP significantly decreased (P>0.05) serum glucose, glycated hemoglobin (HbA1c), malondialdehyde (MDA) levels and significantly increased (P>0.05) serum insulin, the activity of antioxidant enzymes and normalized their mRNA gene expression. Furthermore, SP attenuates STZ-induced upregulation of the gluconeogenic enzyme pyruvate carboxylase (PC), the pro-apoptotic Bax and caspase-3 (CASP-3), tumor necrosis factor alpha (TNF-α) gene expression. The Western blot results revealed that, SP induced dow...
Canadian journal of physiology and pharmacology, 2016
The effect of monosodium glutamate (MSG) on brain tissue and the relative ability of lycopene to ... more The effect of monosodium glutamate (MSG) on brain tissue and the relative ability of lycopene to avert these neurotoxic effects were investigated. Thirty-two male Wistar rats were distributed into 4 groups: group I, untreated (placebo); group II, injected with MSG (5 mg·kg(-1)) s.c.; group III, gastrogavaged with lycopene (10 mg·kg(-1)) p.o.; and group IV received MSG with lycopene with the same mentioned doses for 30 days. The results showed that MSG induced elevation in lipid peroxidation marker and perturbation in the antioxidant homeostasis and increased the levels of brain and serum cholinesterase (ChE), total creatine phosphokinase (CPK), creatine phosphokinase isoenzymes BB (CPK-BB), and lactate dehydrogenase (LDH). Glutathione S-transferase (GST), superoxide dismutase (SOD), and catalase (CAT) activities and gene expression were increased and glutathione content was reduced in the MSG-challenged rats, and these effects were ameliorated by lycopene. Furthermore, MSG induced a...
Corresponding Author: Salah M. Abdel-Rahman, Genetic Engineering and Biotechnology Research Insti... more Corresponding Author: Salah M. Abdel-Rahman, Genetic Engineering and Biotechnology Research Institute (GEBRI), Mubarak City for Scientific Research and Technology Applications, Alexandria, Egypt. Tel.: 002 0112727468, Fax: 002 034593423 E-mail: salahmaa@yahoo. ...
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