Papers by Susanna Pecchia
26° SIPaV Congress, 2021
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International Journal of Molecular Sciences, 2022
Safeguarding food supply in a world environment subject to sudden climate change, reducing the us... more Safeguarding food supply in a world environment subject to sudden climate change, reducing the use of anthropogenic sources of pollution as much as possible, and using crops that must necessarily be increasingly resilient to biotic and abiotic stresses is a mandatory and ambitious necessity for the foreseeable future [...]
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International Journal of Molecular Sciences, 2021
Botrytis cinerea can attack over 500 genera of vascular plants and is considered the second phyto... more Botrytis cinerea can attack over 500 genera of vascular plants and is considered the second phytopathogen in the ‘top ten’ for its economic importance. Traditional fungicides can be ineffective and with increasing fungicide resistance, new sustainable technologies are required. Lately, RNA interference-based fungicides are emerging for their potential uses in crop protection. Therefore, we assessed the potential of this innovative approach targeting the MAP kinase Bmp3 in B. cinerea, a gene involved in saprophytic growth, response to low osmolarity, conidiation, surface sensing, host penetration and lesion formation. After performing a prediction analysis of small interfering RNAs, a 427 nucleotides long dsRNA was selected as construct. We tested the effect of topical applications of dsRNA construct both in vitro by a fungal growth assay in microtiter plates and in vivo on detached lettuce leaves artificially inoculated. In both cases, topical applications of dsRNA led to gene knock...
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Journal of Plant Pathology, 2002
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Journal of Plant Pathology, 2001
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Diaporthe helianthi, the causal agent of sunflower stem canker, a serious pathogen of sunflower i... more Diaporthe helianthi, the causal agent of sunflower stem canker, a serious pathogen of sunflower in Europe, is sporadically recorded in Italy. A comparison of pathogen populations from different countries can be performed in order to detect genetically different biotypes. Our approach was turned to study molecular variability in genomic coding and non coding regions among D.helianthi isolates from different geographic origin. A set of isolates from different countries have been used to evaluate genomic variability in endo-polygalacturonase (endo-PG) genic portions and IGS region of rDNA. A PCR analysis with Fusarium moniliforme endo-PG degenerated primers produced variable amplification profiles between isolates in relation to their geographic origin. French and Yugoslavian isolates (countries where the pathogen is most aggressive) showed conserved patterns, while Italian isolates showed highly variable patterns. Primers for speciation studies in filamentous ascomycetes were used for the amplification of a portion of IGS region of rDNA. Amplification products were purified and sequenced in both directions. Phylogenetic analysis separated the isolates into two main clusters: i) all isolate from France and Yugoslavia; ii) all isolates from Italy. This investigation pointed out a good correlation between data obtained by the two different molecular approaches in order to detect intraspecific genetic variability in D.helianthi
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Papers by Susanna Pecchia