Papers by Renata Karpiskova
Veterinarni Medicina, Aug 31, 2017
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Hygiena, Jun 30, 2023
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Cil prace: Posoudit výskyt antimikrobialni rezistence u kmenů L. monocytogenes izolovaných od lid... more Cil prace: Posoudit výskyt antimikrobialni rezistence u kmenů L. monocytogenes izolovaných od lidi a z potravin Ceske republiky v obdobi let 2001 až 2012. Material a metody: Fenotypový projev antimikrobialni rezistence byl monitorovan diskovou difuzni metodou a doplněn stanovenim minimalni inhibicni koncentrace metodou E-testu a genů rezistence metodou PCR. Výsledky: Vsechny testovane kmeny (678) byly citlive k ampicilinu, penicilinu, gentamicinu, trimetoprimu, vankomycinu a chloramfenikolu. Ojediněle byla u kmenů pochazejicich z potravin prokazana rezistence k tetracyklinu (2/509) a erytromycinu (1/509). Zavěr: Ve sledovanem obdobi byla zjistěna velmi dobra a doposud stabilni citlivost testovaných kmenů L. monocytogenes izolovaných z clověka i potravin k antibiotikům využivaných v terapii listerioz. Sporadický výskyt rezistence byl zaznamenan pouze u kmenů z potravin.
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Epidemiologie Mikrobiologie Imunologie, 2014
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Acta Veterinaria Brno, 2001
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Kolistin je polymyxinove antibiotikum ucinne proti větsině gram-negativnich bakterii. Ve veterina... more Kolistin je polymyxinove antibiotikum ucinne proti větsině gram-negativnich bakterii. Ve veterinarni medicině se použiva k lecbě infekci vyvolaných bakteriemi z celedi Enterobacteriaceae. V soucasnosti se vraci význam kolistinu pro lecbu humannich infekci vyvolaných multirezistentnimi bakteriemi. Mechanismy rezistence ke kolistinu jsou kodovany chromozomalně, ale daleko větsi hrozbu představuje nedavno objevena rezistence nesena na mobilnich genetických elementech. Plazmidy se siři horizontalnim přenosem v ramci druhu i mezidruhově. Gen mcr-1 byl poprve identifikovan v roce 2015 u zviřat v Cině a od te doby byly popsany připady bakterii nesoucich gen mcr-1, ale i dalsi (mcr-2 až mcr-5) po celem světě. Mezinarodni trh se zviřaty a potravinami může být významným zdrojem rozsiřeni tohoto typu rezistence. V Ceske republice zatim nebyl výskyt genů mcr důkladně prozkouman v potravinovem řetězci ani u lidi. V teto studii byl sledovan výskyt genů mcr ve vybraných potravinach z tržni sitě CR.
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Background: The strains of S. aureus ST398 are associated worldwide with livestock, humans and fo... more Background: The strains of S. aureus ST398 are associated worldwide with livestock, humans and food products. They contain a broad spectrum of plasmid-borne genes conferring antibiotic resistance having impact in pathogenicity of the veterinary strains. Objectives: The strains isolated from raw cow’s milk samples were genotypically analyzed, and their plasmids were characterized in detail. Methods: The MLST, spa typing, prophage and virulence genes content were carried out. The whole plasmid sequences were determined in two selected strains. Results: All the host strains of ST398 were resistant to oxacillin and other 2 – 8 antibiotics. The spa type t011 predominated over type t034. All the strains, with excepting of one isolate, harbored the prophage of serogroup A. Plasmids isolated from eight strains were assigned to six REAP types. Two plasmids were sequenced. They showed a different size, REAP fingerprint and a sequence structure. Amplification of the aad gene encoding aminoglycoside 6-adenylyltransferase is conserved in predominated group of small plasmids. One plasmid carrying the resistance gene to lincosamides was unique in the studied strains. It harbored a great region of identical sequences with plasmid of Staphylococcus chromogenes, which indicates on the interspecies horizontal transfer of plasmid genes. Conclusions: This study reports a research data of not yet well described plasmids of clonal ST398 strains of S. aureus having an impact in veterinary medicine and food industry. This work was supported by the grant from Ministry of Agriculture of the Czech Republic (QJ1510216) and from the MUNI/A/0824/2017.
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Eurosurveillance, May 1, 1999
La Republica Checa es un pais situado en la zona central de Europa, cuya poblacion asciende a uno... more La Republica Checa es un pais situado en la zona central de Europa, cuya poblacion asciende a unos 10 millones de habitantes. La salmonelosis es la infeccion intestinal que se declara con mayor frecuencia. Hasta 1989, se declaraban unos 10.000 casos por ano, aislandose un numero similar de Salmonella enteritidis y S. typhimurium. Desde 1989, los casos de salmonelosis humana han aumentado rapidamente. Entre 1993 y 1997, el numero de casos anuales notificado cada ano fluctuo de 39.862 a 54.554, lo que supone una incidencia de 386 a 518 casos por 100.000 habitantes, y alcanzo su punto maximo en 1995. La S. enteritidis represento el 95% de los casos declarados y S. typhimurium, el 3%.
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Eurosurveillance, May 1, 1999
La Repubblica Ceca e un paese dell'Europa Centrale con una popolazione di circa 10 milioni di... more La Repubblica Ceca e un paese dell'Europa Centrale con una popolazione di circa 10 milioni di abitanti. La salmonellosi e l'infezione intestinale notificata piu frequentemente. Fino al1989, sono stati notificati circa 10.000 casi all'anno e Salmonella enteritidis e S. typhimurium sono stati isolati in uguale misura. Dal 1989, i casi di salmonellosi umana sono aumentati rapidamente. Ogni anno, dal 1993 al 1997, sono stati riportati un numero di casi compreso tra 39.862 e 54.554, con un'incidenza compresa tra 386 e 518 casi ogni 100.000 abitanti che ha presentato un picco nel 1995. Il 95% dei casi era dovuto a S. enteritidis e il 3% a S. typhimurium.
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Cil prace: Zhodnoceni výskytu a charakteristik L. monocytogenes ve vzorcich syroveho kravskeho ml... more Cil prace: Zhodnoceni výskytu a charakteristik L. monocytogenes ve vzorcich syroveho kravskeho mleka odebraných na farmach (bazenove vzorky) a z prodejnich automatů. Material a metody: Průkaz a stanoveni poctu bylo provedeno dle CSN EN/ISO 11290 – 1,2. Kmeny byly typizovany serotypizaci a makrorestrikcni analýzou metodou pulzni gelove elektroforezy. Výsledky: Přitomnost L. monocytogenes byla prokazana u 3,2 % (11/346) bazenových vzorků a u 1,8 % (4/219) vzorků syroveho kravskeho mleka z prodejnich automatů. Nalezy L. monocytogenes v syrovem mlece měly sporadický charakter, pouze na jedne farmě byly detekovany opakovaně. Z 16 ziskaných kmenů L. monocytogenes 13 naleželo k serotypu 1/2a, dva kmeny k serotypu 1/2b a jeden k serotypu 4b. Makrorestrikcni analýza ukazala znacnou heterogenitu profilů, celkem bylo detekovano 9 různých pulzotypů. Nejcastěji byl detekovan pulzotyp 711, který byl zjistěn na třech farmach. Zavěr: Výskyt L. monocytogenes v syrovem kravskem mlece v Ceske republice je relativně nizký. Dosažene výsledky potvrdily, že některe klony L. monocytogenes ze syroveho mleka jsou shodne s kmeny izolovanými z potravin a clověka. Cil prace: Zhodnoceni výskytu a charakteristik L. monocytogenes ve vzorcich syroveho kravskeho mleka odebraných na farmach (bazenove vzorky) a z prodejnich automatů. Material a metody: Průkaz a stanoveni poctu bylo provedeno dle CSN EN/ISO 11290 – 1,2. Kmeny byly typizovany serotypizaci a makrorestrikcni analýzou metodou pulzni gelove elektroforezy. Výsledky: Přitomnost L. monocytogenes byla prokazana u 3,2 % (11/346) bazenových vzorků a u 1,8 % (4/219) vzorků syroveho kravskeho mleka z prodejnich automatů. Nalezy L. monocytogenes v syrovem mlece měly sporadický charakter, pouze na jedne farmě byly detekovany opakovaně. Z 16 ziskaných kmenů L. monocytogenes 13 naleželo k serotypu 1/2a, dva kmeny k serotypu 1/2b a jeden k serotypu 4b. Makrorestrikcni analýza ukazala znacnou heterogenitu profilů, celkem bylo detekovano 9 různých pulzotypů. Nejcastěji byl detekovan pulzotyp 711, který byl zjistěn na třech farmach. Zavěr: Výskyt L. monocytogenes v syrovem kravskem mlece v Ceske republice je relativně nizký. Dosažene výsledky potvrdily, že některe klony L. monocytogenes ze syroveho mleka jsou shodne s kmeny izolovanými z potravin a clověka.
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Veterinarni Medicina, 1996
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Eurosurveillance, May 1, 1999
En Republique Tcheque, pays d’Europe Centrale comptant pres de 10 millions d'habitants, les s... more En Republique Tcheque, pays d’Europe Centrale comptant pres de 10 millions d'habitants, les salmonelloses sont responsables de la plupart des infections d'origine alimentaire. Jusqu’en 1989, le nombre de cas rapportes chaque annee s'elevait a 10 000 environ, le nombre de cas de Salmonella enteritidis etant a peu pres egal a celui de S. typhimurium . Depuis 1989, les cas de salmonelloses humaines ont augmente rapidement. Au cours de la periode 1993-1997 le nombre de cas annuels rapporte a fluctue entre 39 862 et 54 554 correspondant a une incidence de 386 a 518 cas par 100 000 habitants, avec un pic en 1995. S. Enteritidis representait 95 % des cas contre 3% pour S. typhimurium.
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PubMed, Sep 1, 2014
This study was conducted to evaluate the contamination of raw pork meat with Staphylococcus aureu... more This study was conducted to evaluate the contamination of raw pork meat with Staphylococcus aureus in retail market and their ability to participate in the creation of foodborne intoxication. Strains were characterized by genotypic traits. Of the 197 samples examined 43 (21.8%) were found to be positive for the presence of S. aureus. Toxigennic properties were found in 21 (48.8%) isolates, the major enterotoxigennic gene found was seh (81%). All the S. aureus isolates were screened for resistance to antimicrobial agents by disk diffusion method and for mecA gene encoding resistance to meticillin. Two of the strains isolated in this study harboured the mecA gene (MRSA). The determination of sequence type 398 (ST398) has been confirmed in both MRSA isolates.
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PubMed, Apr 1, 2003
Salmonelloses belong to the most important alimentary diseases. From 1989 the main source of infe... more Salmonelloses belong to the most important alimentary diseases. From 1989 the main source of infection is S. Enteritidis (95% and more). The ratio of S. Typhimurium in the etiology declined gradually since 1994 from 3% in 1993 to 1.9% in 2001. The number of detected multiresistant strains STM DT104 is so far low in the Czech Republic. In 2000 laboratory tests confirmed 53 and in 2001 a total of 63 strains. Analysis of these strains revealed: 1. the proportion of multiresistant strains STM DT104 of the total number of isolated STM strains increased from 7% in 2000 to 11.5% in 2001, 2. the territory in the CR where these multiresistant strains were found expanded, 3. the relationship of proportions of the most frequently detected STM phagotypes in the human population changes: in 2000 this ratio was balanced, in 2001 the ratio of phagotype DT104 is roughly three times higher than of DT 141, 4. analysis of all STM strains from the environment and animals indicates that the frequency of STM DT104 is 73%. Almost 90% of these strains are multiresistant (resistant to one or several antimicrobial substances). The highest number was found in calves, piglets but also in poultry. With regard to the results of the submitted work it is important to concentrate attention on detection and further laboratory examination of STM strains in the human and animal population.
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PubMed, Apr 1, 1998
The authors pay attention to the mode of transmission of salmonelloses in children under two year... more The authors pay attention to the mode of transmission of salmonelloses in children under two years of age, where the highest specific morbidity was recorded. Using phenotypic (biochemical properties, sensitivity to antibiotics, phagotype) and genotypic properties (presence of plasmids) they examine five sporadic and seven epidemic incidences of salmonelloses in young children. The most frequent agent is Salmonella enteritidis, phagotype 8, plasmid 55 kb. Strains of S. enteritidis are isolated from the faeces of affected children, their family contacts, from food and the sick child's environment. Alimentary transmission by the incriminated food was detected from epidemic incidence where the vehicle were confectionery products contaminated by S. enteritidis. In sporadic cases in families also direct and indirect contact may have participated in the transmission of infection.
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PubMed, Feb 1, 2000
In the Czech Republic in 1989 a triple increase of the incidence of salmonelloses was recorded: 3... more In the Czech Republic in 1989 a triple increase of the incidence of salmonelloses was recorded: 34,435 cases. Since that year the morbidity trend varies between 400 and 500 cases per 100,000 population. The dominating agent (95% and more) is Salmonella Enteritidis PT8. The epidemic incidence was recorded on the whole territory of the Czech Republic mainly in Moravia and in the East Bohemian region. The specific morbidity is highest in 0 and 1-4 year-old children. The seasonal incidence has two peaks with the exception of 1997. During the period between 1989 and 1996 there was a significant increase of epidemics of salmonellosis in conjunction with food production incl. private confectionery shops, restaurants and the sale of foods in the streets. The most important vehicle are eggs and egg products, in particular confectionery. Salmonella Typhimurium DT104 is found only rarely in the Czech Republic. The first epidemic (15 cases) developed in 1998.
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Papers by Renata Karpiskova