Journal of the American Dental Association, Jun 1, 1987
Crown contours represent a group of characteristics critical for the longevity and success of den... more Crown contours represent a group of characteristics critical for the longevity and success of dental restorations. This article presents the theories that have been developed about crown contours, describes each feature in detail, and clarifies potential interrelationships with all components of the mouth. When identified early, crown contours can be incorporated in treatment planning and restorative procedures.
In this cross-sectional study, risk and potentially causative factors of gingival recession were ... more In this cross-sectional study, risk and potentially causative factors of gingival recession were examined and their relationship to apical migration of the gingival margin evaluated. Thirty eight patients (18-60 years), displaying one or more sites with gingival recession but without any significant periodontal disease participated. A total of 28 parameters were evaluated in both 'test' teeth (50 teeth with gingival recession) and 'control' teeth (50 contralateral teeth). The results revealed that gingival margin recession was associated with both high inflammatory and plaque scores, with decreased widths of keratinized and attached gingiva and with the subjects' toothbrush bristle hardness.
Stem Cells residing in the Apical Papilla (SCAP) of human permanent teeth represent a promising c... more Stem Cells residing in the Apical Papilla (SCAP) of human permanent teeth represent a promising cell source for dental tissue regeneration. Therefore, the functional and molecular properties of specific subpopulations existing within heterogeneous cultures should be further investigated to give insight whether their selection could be beneficial for targeted therapeutic applications. In this study we extensively characterized SCAP cultures established from 10 healthy subjects, as well as their STRO-1(pos/)CD146(pos) and STRO-1(neg/)CD146(pos) subpopulations isolated with fluorescence-activated cell sorting. SCAP were analyzed for embryonic (Nanog, Oct3/4, SSEA-3, TRA-1-60), mesenchymal (STRO-1, CD146/MUC18, CD105/endoglin, CD24, CD90/Thy-1, CD81-TAPA, CD34, CD49f/a6-integrin), neural (CD271/NGFR, nestin) and hematopoietic (CD117/c-kit, CD45) stem cell (SC) markers using flow cytometry. Multipotentiality was evaluated with culture specific staining (Alizarin-Red-S, Oil- Red-O) and RT-PCR analysis for osteo/odontogenic (DSPP, BSP, ALP, osteocalcin, osteonectin, BMP-2, Runx2), adipogenic (lipoprotein-lipase-LPL) and neurogenic (Neurofilament/NFL-L, nestin, β-tubulin-III, NCAM) markers. Our results showed that the STRO-1(pos)/CD146(pos) subpopulation demonstrated higher CFU efficiency and much higher expression of several embryonic and mesenchymal SC markers compared to the non-sorted SCAP. They also showed enhanced odontogenic differentiation potential, as evidenced by higher mineralization capacity and expression of osteo/odontogenic markers. By contrast, absence of STRO-1 in the STRO-1(neg)/CD146(pos) subpopulation yielded the opposite results and was associated with significant downgrading of the above-mentioned properties. These results suggest that STRO-1(pos)/CD146(pos) SCAP cells represent a very promising adult MSCs source with enhanced multipotent SC properties that could be easily isolated with simple flow cytometric methods to be used for tissue engineering applications.
Revista internacional de prótesis estomatológica, 2004
Objetivo: Este estudio de laboratorio ha comparado la resistencia adhesiva a la cizalla entre tre... more Objetivo: Este estudio de laboratorio ha comparado la resistencia adhesiva a la cizalla entre tres resinas compuestas indirectas y una aleacion noble, mediante sus respectivos sistemas adhesivos. Material y metodos: Se dividio de forma equitativa en cuatro grupos a 120 discos colados en una aleacion de contenido medio en oro, de metales de alta nobleza y ceramica (V-Deltaloy), y se realizaron diferentes tratamientos de recubrimiento: se coloco porcelana feldespatica convencional (Omega) sobre un juego de muestras para hacer de controles, y en los otros grupos se emplearon tres resinas compuestas indirectas (Artglass, Sculpture, Targis) con sus respectivos sistemas adhesivos. Se examinaron las muestras en una prueba de cizalla en paralelo, la mitad de las cuales tras 24 horas de conservacion en seco a temperatura ambiente y el resto despues de 10 dias de conservacion en solucion salina normal a 37oC y termociclado. Se evaluaron las muestras fracturadas con el fin de determinar la naturaleza del fracaso. Resultados: Los valores de resistencia adhesiva media a la cizalla (en MPa), antes y despues de la conservacion en medio humedo y el termociclado, fueron de 30 y 23 para el grupo de metal-ceramica, 29 y 23 para el grupo de Artglass, 20 y 19 para el grupo de Sculpture y 17 y 14 para el grupo de Targis, respectivamente. Los grupos de metal-ceramica y Artglass mostraron una resistencia adhesiva significativamente mayor que la de los otros dos grupos. Todas las muestras, con la excepcion del grupo de Sculpture, presentaron una disminucion significativa en la resistencia adhesiva tras la conservacion en medio humedo y el termociclado. Conclusiones: Ningun grupo supero la resistencia adhesiva a la cizalla del grupo de metal-ceramica, pero el grupo de Artglass con su respectivo sistema adhesivo metal-resina mostro una resistencia adhesiva similar. El grupo de Sculpture presento una adhesion estable despues de la conservacion en medio humedo y el termociclado.
There is substantial evidence supporting the anti-inflammatory and regenerative potential of dent... more There is substantial evidence supporting the anti-inflammatory and regenerative potential of dental pulp stem cells (DPSCs) through direct cell transplantation or paracrine action. However, DPSC secretome profile remains inadequately studied. This study provides proteomic profiling of the human DPSC secretome by comparatively analysising cell lysates and respective culture supernatants (i.e. conditioned media-CM) under variable oxygen tension conditions (normoxia-20% O2/CM_Norm vs. hypoxia 2% O2/CM_Hyp) and/or stimulation with Tumor Necrosis Factor alpha (TNF-α). DPSC-CM samples and respective crude lysates (DPSC-CL) were collected and subjected to SDS-PAGE, followed by LC-MS/MS analysis. The identified proteins were analyzed by Gene Ontology, Reactome, and String databases. The anti-inflammatory properties of DPSC-CMs were validated via an in vitro RAW_246.7 murine macrophages model through evaluation of the expression of pro-and anti-inflammatory markers by real-time PCR. Results showed a total of 2413 proteins identified in CM_Norm, 2479 in CM_Norm+TNF-α, 1642 in CM_Hyp, and 2002 in CM_Hyp + TNF-α samples. CM_Norm contained 122 proteins statistically significantly upregulated compared to the CM_Hyp and involved in pathways related to "ECM organization", "cellular response to hypoxia", and "IL signaling". Functional network analysis showed that TGFβ1, TIMP1 and TIMP2 were key nodes among proteins significantly upregulated in the CM_Norm compared to the CM_Hyp, interacting with more than 10 proteins, each. DPSC-CM application in the in vitro RAW_246.7 model decreased the expression of pro-inflammatory markers (MMP-3, MMP-9, MMP-13, MCP-1), while increasing anti-inflammatory markers (IL-10). Overall, DPSC-CM collected under normoxic conditions is enriched with anti-inflammatory, tissue repair and regenerative factors, which prompts further investigation on its therapeutic applications.
Journal of the American Dental Association, Jun 1, 1987
Crown contours represent a group of characteristics critical for the longevity and success of den... more Crown contours represent a group of characteristics critical for the longevity and success of dental restorations. This article presents the theories that have been developed about crown contours, describes each feature in detail, and clarifies potential interrelationships with all components of the mouth. When identified early, crown contours can be incorporated in treatment planning and restorative procedures.
In this cross-sectional study, risk and potentially causative factors of gingival recession were ... more In this cross-sectional study, risk and potentially causative factors of gingival recession were examined and their relationship to apical migration of the gingival margin evaluated. Thirty eight patients (18-60 years), displaying one or more sites with gingival recession but without any significant periodontal disease participated. A total of 28 parameters were evaluated in both 'test' teeth (50 teeth with gingival recession) and 'control' teeth (50 contralateral teeth). The results revealed that gingival margin recession was associated with both high inflammatory and plaque scores, with decreased widths of keratinized and attached gingiva and with the subjects' toothbrush bristle hardness.
Stem Cells residing in the Apical Papilla (SCAP) of human permanent teeth represent a promising c... more Stem Cells residing in the Apical Papilla (SCAP) of human permanent teeth represent a promising cell source for dental tissue regeneration. Therefore, the functional and molecular properties of specific subpopulations existing within heterogeneous cultures should be further investigated to give insight whether their selection could be beneficial for targeted therapeutic applications. In this study we extensively characterized SCAP cultures established from 10 healthy subjects, as well as their STRO-1(pos/)CD146(pos) and STRO-1(neg/)CD146(pos) subpopulations isolated with fluorescence-activated cell sorting. SCAP were analyzed for embryonic (Nanog, Oct3/4, SSEA-3, TRA-1-60), mesenchymal (STRO-1, CD146/MUC18, CD105/endoglin, CD24, CD90/Thy-1, CD81-TAPA, CD34, CD49f/a6-integrin), neural (CD271/NGFR, nestin) and hematopoietic (CD117/c-kit, CD45) stem cell (SC) markers using flow cytometry. Multipotentiality was evaluated with culture specific staining (Alizarin-Red-S, Oil- Red-O) and RT-PCR analysis for osteo/odontogenic (DSPP, BSP, ALP, osteocalcin, osteonectin, BMP-2, Runx2), adipogenic (lipoprotein-lipase-LPL) and neurogenic (Neurofilament/NFL-L, nestin, β-tubulin-III, NCAM) markers. Our results showed that the STRO-1(pos)/CD146(pos) subpopulation demonstrated higher CFU efficiency and much higher expression of several embryonic and mesenchymal SC markers compared to the non-sorted SCAP. They also showed enhanced odontogenic differentiation potential, as evidenced by higher mineralization capacity and expression of osteo/odontogenic markers. By contrast, absence of STRO-1 in the STRO-1(neg)/CD146(pos) subpopulation yielded the opposite results and was associated with significant downgrading of the above-mentioned properties. These results suggest that STRO-1(pos)/CD146(pos) SCAP cells represent a very promising adult MSCs source with enhanced multipotent SC properties that could be easily isolated with simple flow cytometric methods to be used for tissue engineering applications.
Revista internacional de prótesis estomatológica, 2004
Objetivo: Este estudio de laboratorio ha comparado la resistencia adhesiva a la cizalla entre tre... more Objetivo: Este estudio de laboratorio ha comparado la resistencia adhesiva a la cizalla entre tres resinas compuestas indirectas y una aleacion noble, mediante sus respectivos sistemas adhesivos. Material y metodos: Se dividio de forma equitativa en cuatro grupos a 120 discos colados en una aleacion de contenido medio en oro, de metales de alta nobleza y ceramica (V-Deltaloy), y se realizaron diferentes tratamientos de recubrimiento: se coloco porcelana feldespatica convencional (Omega) sobre un juego de muestras para hacer de controles, y en los otros grupos se emplearon tres resinas compuestas indirectas (Artglass, Sculpture, Targis) con sus respectivos sistemas adhesivos. Se examinaron las muestras en una prueba de cizalla en paralelo, la mitad de las cuales tras 24 horas de conservacion en seco a temperatura ambiente y el resto despues de 10 dias de conservacion en solucion salina normal a 37oC y termociclado. Se evaluaron las muestras fracturadas con el fin de determinar la naturaleza del fracaso. Resultados: Los valores de resistencia adhesiva media a la cizalla (en MPa), antes y despues de la conservacion en medio humedo y el termociclado, fueron de 30 y 23 para el grupo de metal-ceramica, 29 y 23 para el grupo de Artglass, 20 y 19 para el grupo de Sculpture y 17 y 14 para el grupo de Targis, respectivamente. Los grupos de metal-ceramica y Artglass mostraron una resistencia adhesiva significativamente mayor que la de los otros dos grupos. Todas las muestras, con la excepcion del grupo de Sculpture, presentaron una disminucion significativa en la resistencia adhesiva tras la conservacion en medio humedo y el termociclado. Conclusiones: Ningun grupo supero la resistencia adhesiva a la cizalla del grupo de metal-ceramica, pero el grupo de Artglass con su respectivo sistema adhesivo metal-resina mostro una resistencia adhesiva similar. El grupo de Sculpture presento una adhesion estable despues de la conservacion en medio humedo y el termociclado.
There is substantial evidence supporting the anti-inflammatory and regenerative potential of dent... more There is substantial evidence supporting the anti-inflammatory and regenerative potential of dental pulp stem cells (DPSCs) through direct cell transplantation or paracrine action. However, DPSC secretome profile remains inadequately studied. This study provides proteomic profiling of the human DPSC secretome by comparatively analysising cell lysates and respective culture supernatants (i.e. conditioned media-CM) under variable oxygen tension conditions (normoxia-20% O2/CM_Norm vs. hypoxia 2% O2/CM_Hyp) and/or stimulation with Tumor Necrosis Factor alpha (TNF-α). DPSC-CM samples and respective crude lysates (DPSC-CL) were collected and subjected to SDS-PAGE, followed by LC-MS/MS analysis. The identified proteins were analyzed by Gene Ontology, Reactome, and String databases. The anti-inflammatory properties of DPSC-CMs were validated via an in vitro RAW_246.7 murine macrophages model through evaluation of the expression of pro-and anti-inflammatory markers by real-time PCR. Results showed a total of 2413 proteins identified in CM_Norm, 2479 in CM_Norm+TNF-α, 1642 in CM_Hyp, and 2002 in CM_Hyp + TNF-α samples. CM_Norm contained 122 proteins statistically significantly upregulated compared to the CM_Hyp and involved in pathways related to "ECM organization", "cellular response to hypoxia", and "IL signaling". Functional network analysis showed that TGFβ1, TIMP1 and TIMP2 were key nodes among proteins significantly upregulated in the CM_Norm compared to the CM_Hyp, interacting with more than 10 proteins, each. DPSC-CM application in the in vitro RAW_246.7 model decreased the expression of pro-inflammatory markers (MMP-3, MMP-9, MMP-13, MCP-1), while increasing anti-inflammatory markers (IL-10). Overall, DPSC-CM collected under normoxic conditions is enriched with anti-inflammatory, tissue repair and regenerative factors, which prompts further investigation on its therapeutic applications.
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