The gill of teleost fish is a multifunctional organ involved in many physiological processes, inc... more The gill of teleost fish is a multifunctional organ involved in many physiological processes, including protection of the mucosal gill surface against pathogens and other environmental antigens by the gill-associated lymphoid tissue (GIALT). Climate change associated phenomena, such as increasing frequency and magnitude of harmful algal blooms (HABs) put extra strain on gill function, contributing to enhanced fish mortality and fish kills. However, the molecular basis of the HAB-induced gill injury remains largely unknown due to the lack of high-throughput transcriptomic studies performed on teleost fish in laboratory conditions. We used juvenile rainbow trout (Oncorhynchus mykiss) to investigate the transcriptomic responses of the gill tissue to two (high and low) sublethal densities of the toxin-producing alga Prymnesium parvum, in relation to non-exposed control fish. The exposure time to P. parvum (4–5 h) was sufficient to identify three different phenotypic responses among the ...
K. Einer-Jensen, S. Biacchesi, A. Stegmann, M. Bremont and N. Lorenzen National Veterinary Instit... more K. Einer-Jensen, S. Biacchesi, A. Stegmann, M. Bremont and N. Lorenzen National Veterinary Institute, DTU, Aarhus, Denmark INRA, Jouy, France Viral haemorrhagic septicaemia virus (VHSV) is the economically most important viral disease in European rainbow trout farming. The virus was introduced to fresh water farms in the 1950ies from a reservoir of VHSV in the marine environment. Isolates from wild marine fish and fresh water farms are difficult to distinguish serologically but they show different virulence profiles: marine isolates typically cause little or no mortality in rainbow trout fry following experimental waterborne challenge, while freshwater isolates often kill the majority of the fish. Genetic analysis reveal that the change in host range (to include rainbow trout) likely have occurred several times. Virus from the marine environment therefore continues to represent a threat to the expanding trout aquaculture industry in the marine environment. Identification of potentia...
DNA vaccines encoding the glycoprotein genes of the salmonid rhabdoviruses VHSV and IHNV are very... more DNA vaccines encoding the glycoprotein genes of the salmonid rhabdoviruses VHSV and IHNV are very efficient in eliciting protective immune responses against their respective diseases in rainbow trout (Oncorhynchus mykiss). The early anti-viral response (EAVR) provides protection by 4 days post vaccination and is non-specific and transient while the specific anti-viral response (SAVR) is long lasting and highly specific. Since both VHSV and IHNV are endemic in rainbow trout in several geographical regions of Europe and Atlantic salmon (Salmo salar) on the Pacific coast of North America, co-vaccination against the two diseases would be a preferable option. In the present study we demonstrated that a single injection of mixed DNA vaccines induced long-lasting protection against both individual and a simultaneous virus challenge 80 days post vaccination. Transfected muscle cells at the injection site expressed both G proteins. This study confirms the applied potential of using a combine...
DNA vaccination in fish promotes an early chemokine-related recruitment of B cells to the muscle ... more DNA vaccination in fish promotes an early chemokine-related recruitment of B cells to the muscle Castro, R., Martínez-Alonso, S., Fischer, U., Álvarez de Haro, N., Soto-Lampe, V., Lorenzen, N., Lorenzen, E., Wang, T., Secombes, C. J. & Tafalla, C. 2013 In : Fish and Shellfish Immunology. 34, 6, p. 1643 1 p., O-124 Publication: Research peer-review › Conference abstract in journal – Annual report year: 2013
Under Experimental Conditions DTU Orbit (27/03/2019) Improved Protection of Rainbow Trout Against... more Under Experimental Conditions DTU Orbit (27/03/2019) Improved Protection of Rainbow Trout Against Furunculosis by an Autologous Vaccine Under Experimental Conditions Despite vaccination with oil-adjuvanted vaccines against vibriosis and furunculosis, sea reared rainbow trout in Denmark often encounter outbreaks of furunculosis during warm summer periods. To address this issue under experimental conditions, two groups of rainbow trout were vaccinated by i.p. injection with two different oil-adjuvanted vaccines: (1) a commercial vaccine comprising Vibrio anguillarum serotype O1 and O2, and Aeromonas salmonicida subspecies salmonicida bacterins, with all bacteria originating from Atlantic salmon, and (2) an experimental vaccine based on cultures of the same bacterial species originating from rainbow trout reared in Danish sea farms. The experiment also included a third group of non-vaccinated controls. All fish were individually chip-tagged to allow mixing of all groups in three replic...
The gill of teleost fish is a multifunctional organ involved in many physiological processes, inc... more The gill of teleost fish is a multifunctional organ involved in many physiological processes, including protection of the mucosal gill surface against pathogens and other environmental antigens by the gill-associated lymphoid tissue (GIALT). Climate change associated phenomena, such as increasing frequency and magnitude of harmful algal blooms (HABs) put extra strain on gill function, contributing to enhanced fish mortality and fish kills. However, the molecular basis of the HAB-induced gill injury remains largely unknown due to the lack of high-throughput transcriptomic studies performed on teleost fish in laboratory conditions. We used juvenile rainbow trout (Oncorhynchus mykiss) to investigate the transcriptomic responses of the gill tissue to two (high and low) sublethal densities of the toxin-producing alga Prymnesium parvum, in relation to non-exposed control fish. The exposure time to P. parvum (4–5 h) was sufficient to identify three different phenotypic responses among the ...
K. Einer-Jensen, S. Biacchesi, A. Stegmann, M. Bremont and N. Lorenzen National Veterinary Instit... more K. Einer-Jensen, S. Biacchesi, A. Stegmann, M. Bremont and N. Lorenzen National Veterinary Institute, DTU, Aarhus, Denmark INRA, Jouy, France Viral haemorrhagic septicaemia virus (VHSV) is the economically most important viral disease in European rainbow trout farming. The virus was introduced to fresh water farms in the 1950ies from a reservoir of VHSV in the marine environment. Isolates from wild marine fish and fresh water farms are difficult to distinguish serologically but they show different virulence profiles: marine isolates typically cause little or no mortality in rainbow trout fry following experimental waterborne challenge, while freshwater isolates often kill the majority of the fish. Genetic analysis reveal that the change in host range (to include rainbow trout) likely have occurred several times. Virus from the marine environment therefore continues to represent a threat to the expanding trout aquaculture industry in the marine environment. Identification of potentia...
DNA vaccines encoding the glycoprotein genes of the salmonid rhabdoviruses VHSV and IHNV are very... more DNA vaccines encoding the glycoprotein genes of the salmonid rhabdoviruses VHSV and IHNV are very efficient in eliciting protective immune responses against their respective diseases in rainbow trout (Oncorhynchus mykiss). The early anti-viral response (EAVR) provides protection by 4 days post vaccination and is non-specific and transient while the specific anti-viral response (SAVR) is long lasting and highly specific. Since both VHSV and IHNV are endemic in rainbow trout in several geographical regions of Europe and Atlantic salmon (Salmo salar) on the Pacific coast of North America, co-vaccination against the two diseases would be a preferable option. In the present study we demonstrated that a single injection of mixed DNA vaccines induced long-lasting protection against both individual and a simultaneous virus challenge 80 days post vaccination. Transfected muscle cells at the injection site expressed both G proteins. This study confirms the applied potential of using a combine...
DNA vaccination in fish promotes an early chemokine-related recruitment of B cells to the muscle ... more DNA vaccination in fish promotes an early chemokine-related recruitment of B cells to the muscle Castro, R., Martínez-Alonso, S., Fischer, U., Álvarez de Haro, N., Soto-Lampe, V., Lorenzen, N., Lorenzen, E., Wang, T., Secombes, C. J. & Tafalla, C. 2013 In : Fish and Shellfish Immunology. 34, 6, p. 1643 1 p., O-124 Publication: Research peer-review › Conference abstract in journal – Annual report year: 2013
Under Experimental Conditions DTU Orbit (27/03/2019) Improved Protection of Rainbow Trout Against... more Under Experimental Conditions DTU Orbit (27/03/2019) Improved Protection of Rainbow Trout Against Furunculosis by an Autologous Vaccine Under Experimental Conditions Despite vaccination with oil-adjuvanted vaccines against vibriosis and furunculosis, sea reared rainbow trout in Denmark often encounter outbreaks of furunculosis during warm summer periods. To address this issue under experimental conditions, two groups of rainbow trout were vaccinated by i.p. injection with two different oil-adjuvanted vaccines: (1) a commercial vaccine comprising Vibrio anguillarum serotype O1 and O2, and Aeromonas salmonicida subspecies salmonicida bacterins, with all bacteria originating from Atlantic salmon, and (2) an experimental vaccine based on cultures of the same bacterial species originating from rainbow trout reared in Danish sea farms. The experiment also included a third group of non-vaccinated controls. All fish were individually chip-tagged to allow mixing of all groups in three replic...
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