Papers by Nehaya Al-Karablieh
Acta horticulturae, Jun 1, 2008
Acta horticulturae, Jun 1, 2008
International Journal of Molecular Sciences, Feb 19, 2009
Microbial biotechnology, Feb 17, 2009
Archives of Phytopathology and Plant Protection, Feb 7, 2020
Journal of Applied Microbiology, Sep 1, 2022
Plant Health Progress, Jan 18, 2023
outer membrane protein TolC is required for
Journal of Microbial & Biochemical Technology, Dec 6, 2017
Jordan Journal of Agricultural Sciences
Fruit rotting causes severe losses in cucumber crop grown in green houses in Jordan, rotting symp... more Fruit rotting causes severe losses in cucumber crop grown in green houses in Jordan, rotting symptom is a common symptom for many bacterial infections. Therefore, the aim of this study was to precisely identify the bacterial causal agent which can be regarded as emergent causal agent in Jordan. Infected cucumber fruits with rotting symptom were sampled from different regions in Jordan, the samples were used in bacterial isolation and screening for putative plant pathogenic bacteria by LOPAT determinative tests. Nine representative Isolates were subjected to pathogenicity test, biochemical characterization and Identification by 16S-rDNA sequencing and in silico digestion by SacI and HinfI restriction enzymes. The isolates were characterized by production of yellowish mucoid materials on Mannitol-Glutamate (MG) medium, reproduce similar rotting symptoms on cucumber fruits as the field infection, typical LOPAT profile and similar biochemical profile to P. viridiflava, occurrence of seven sites for HinfI and absence of SacI site in the16S-rDNA and -based on 16S-rDNA sequences- it had been clustered with P. viridiflava stains. Therefore, it can be concluded that the causal agent of cucumber fruit rotting in Jordan was P. viridiflava.
Journal of Applied Microbiology
Biological Chemistry
Increasing antibiotic resistance in Gram-negative bacteria has mandated the development of both n... more Increasing antibiotic resistance in Gram-negative bacteria has mandated the development of both novel antibiotics and alternative therapeutic strategies. Evidence of interplay between several gastrointestinal peptides and the gut microbiota led us to investigate potential and broad-spectrum roles for the incretin hormone, human glucose-dependent insulinotropic polypeptide (GIP) against the Enterobacteriaceae bacteria, Escherichia coli and Erwinia amylovora. GIP had a potent disruptive action on drug efflux pumps of the multidrug resistant bacteria E. coli TG1 and E. amylovora 1189 strains. The effect was comparable to bacterial mutants lacking the inner and outer membrane efflux pump factor proteins AcrB and TolC. While GIP was devoid of direct antimicrobial activity, it has a potent membrane depolarizing effect, and at low concentrations, it significantly potentiated the activity of eight antibiotics and bile salt by reducing MICs by 4-8-fold in E. coli TG1 and 4-20-fold in E. amyl...
Letters in Applied Microbiology
Jordan Journal of Agricultural Sciences, 2018
Pathogenic Pseudomonas viridiflava has been recently isolated from diseased cucumber plants with ... more Pathogenic Pseudomonas viridiflava has been recently isolated from diseased cucumber plants with fruit rotting symptoms. The pathogen is an opportunistic pathogen with wide host range, producing pyoverdins siderophores type. The aim of this study was to isolate antagonistic bacteria for P. viridiflava from phyllosphere of healthy cucumber plants. Screening for antagonistic bacteria was conducted by bacterial isolation and agar plate antagonistic assay. The potential antagonistic isolates were subjected to pathogenicity test, biochemical characterization and identification by 16S-rDNA sequencing. Among the yellowish fluorescent bacteria isolated, four isolates showed potential antagonistic activity against P. viridiflava. One selected isolate NK2 was subjected to siderophore production analysis. Biochemical characterization and identification by 16S-rDNA sequencing revealed that the isolates belonged to non- pathogenic species P. fluorescens biovar 1 group. Qualitative and quantitati...
Phytopathologia Mediterranea, 2002
Field inspection indicated that crown gall disease occurs in various fruit-tree-growing areas in ... more Field inspection indicated that crown gall disease occurs in various fruit-tree-growing areas in Jordan, including Almafraq, Alsalt, Alshobak, Altafila, Alyadoda, Amman, Irbid, Jerash, Jordan Valley and Madaba. Twohundred tumorigenic Agrobacterium isolates were obtained from stone fruit rootstocks (bitter almond, GF677, GF305, Myrobalan, Nemaguard and Mahaleb), pome fruit rootstocks (apple seedling, quince, and clonal rootstock MM106), grapevine, olive, pomegranate, carob, rose and Cichorium pumilum. The pathogenicity of these isolates was proved through artificial inoculation on tomato seedlings and Kalanchoe plants, and through tmr gene detection with PCR. Biochemical and physiological testing of the isolates indicated the occurrence of biovar 1 (60.5%), biovar 2 (23.5%), and biovar 3 (1%), in addition to an intermediate biovar (15%). Seventy-seven percent of isolates were found to be sensitive to agrocin 84; of these, most (66.9%) belonged to biovar 1.
The Journal of Infection in Developing Countries, 2019
Introduction: Resistance against commonly used antibacterial agents has become a globally recogni... more Introduction: Resistance against commonly used antibacterial agents has become a globally recognized threat to human health. Therefore, the development of new and effective antibacterial agents is necessary to treat infections caused by resistant bacterial strains; plants are a promising source of new agents to be tested. Methodology: The minimum inhibitory concentrations (MIC) of ethanolic extracts of Erodium gruinum, Euphorbia hierosolymitana, Logoecia cuminoides, and Tamarix tetragyna against 10 Gram-negative and 5 Gram-positive bacteria were determined using agar well diffusion and microtiter plate dilution methods, respectively. The phytochemical composition of the crude extracts of the plants was determined using HPLC. Results: Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae, Proteus mirabilis, and Acinetobacter baumannii were sensitive to E. gruinum and E. hierosolymitana extracts. P. aeruginosa ATCC 27853 and M. catarrhalis were sensitive to L. cuminoides extract. P...
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Papers by Nehaya Al-Karablieh