Spherical alginate-based hydrogels have been applied for environmental pollutants removal from aq... more Spherical alginate-based hydrogels have been applied for environmental pollutants removal from aqueous solutions. In this study, zinc alginate beads were prepared using an ionotropic gelation process. The wet hydrogels prepared in various operational conditions were studied to remove eosin-B dye. The effect of operational variables such as pH, initial concentration of eosin-B, contact time, dose of synthesized hydrogel beads, and environmental temperature on the eosin-B removal process was evaluated. The maximum dye removal efficiency was obtained by zinc alginate biosorbent at pH = 3, contact time of 40 min, and 35 mg of biosorbent dose. The maximum adsorption capacity of zinc alginate beads for eosin-B uptake was 53.48 mg/g of biosorbent. The mechanism, kinetics, isotherms, and thermodynamic studies of eosin-B sorption by zinc alginate hydrogels were investigated. Based on the results, the biosorption process follows pseudo-second-order kinetics. The experimental data of the biosorption process are matched with the Langmuir isotherm model, suggesting that monolayer adsorption plays a vital role in the analyte biosorption. According to the thermodynamic studies, the parameters ∆H° (+4679.12 J.mol-1), ∆G° (from -173.45 to -499.13 J.mol-1), and ΔS° (+16.284 J.mol-1.K-1) confirmed the endothermic, spontaneity and randomness of the biosorption process, respectively. This study shows that zinc alginate can be a suitable biosorbent for removing eosin-B from aqueous solutions.
Pearlescent pigments encompass a diverse range known for their ability to create an aesthetically... more Pearlescent pigments encompass a diverse range known for their ability to create an aesthetically pleasing optical appearance due to the facile alignment of numerous platelet-like particles. Consequently, they serve as an ideal choice for imparting robust, lustrous color effects in powder coatings. The synthesis of green pearlescent pigments was conducted in this study, utilizing mica (biotite) and mica-titania flakes coated with a copper oxide thin film. The process entailed the uniform formation of copper (II) chloride through the addition of sodium or potassium hydroxide in the existence of titania, mica, mica-alumina, ammonium molybdate, or mica-titania flakes. Biotite, denoted by the formula K(Mg/Fe)3(A1Si3O10)(OH)2, was selected as the supporting mica. The synthesized pigments were characterized using X-ray diffractometry (XRD) and X-ray fluorescence (XRF) techniques. Evaluations were conducted using polypropylene extrusion with the incorporated pigments, revealing acceptable chemical resistance and distinctive pearl luster effects. The XRD pattern of the Cu/mica pigment exhibited the presence of copper hydroxide covering the mica surface, confirming the formation of the green pearlescent pigments.
This study focused on the optimal production feasibility of distilled water from the concentrate ... more This study focused on the optimal production feasibility of distilled water from the concentrate of a reverse osmosis device using an evacuated tube collector basin solar still. The characteristic effect of the device's condenser on the produced freshwater volume was evaluated to improve the performance of the solar still, resulting in productivity enhancement. The direct contact of two faces of the cubic condenser with water vapor results in the doubling of the effective surface area. However, freshwater production was decreased by eight times compared to the case where only one face of the condenser is in contact with water vapor. This reduction was attributed to the shorter path taken by the condensed droplets. At the bottom of the evacuated tube collector, some building mineral pumice was used to prevent the formation of large water vapor bubbles. According to this methodology, the average daily production of distilled water from the concentrate of the reverse osmosis unit with a total dissolved solids of 15200 ppm was achieved at 4.58 ± 0.12 L.m − 2. The quality of produced distilled water revealed that solar desalination using an evacuated tube collector basin solar still can be a notable and economical solution for the concentrate treatment of reverse osmosis.
The solar still, in which saline or brackish water is converted into freshwater using renewable s... more The solar still, in which saline or brackish water is converted into freshwater using renewable solar energy, can be an effective solution to water scarcity. Inclined solar stills are well-known due to their high production yields compared to other types of solar stills. In this study, an inclined weir-type solar still was developed as a modified inclined solar still with an emphasis on the effect of structural parameters, such as weir height, distance between weirs, and distance between the absorber plate and the condenser cover, on distilled water production. The optimal values of structural parameters for the maximum freshwater production were specified by the response surface methodology and a quadratic model derived from a Box-Behnken design model. The results showed that the operational variables of the inclined weir-type solar still system significantly impacted the water production volume. Using a weir height of 2 cm, a distance of 3.5 cm between weirs, and a distance of 15 cm between the absorber plate and the condenser cover, the maximum water production volume was achieved at 6.474 kg m − 2. day − 1. The selected model had R 2 , R 2 adj and R 2 pred values of 0.9978, 0.9939, and 0.9756, respectively. The maximum and minimum volumes of the produced water were obtained as 6.64125 and 1.79792 kg m − 2. day 1 , based on the multiple response prediction of the variables. According to the validation tests, the model's accuracy in prediction of the maximum amount of freshwater production was notable (97.49%).
Biomass extracted from different plant parts can play a role as a cheap, efficient and eco-friend... more Biomass extracted from different plant parts can play a role as a cheap, efficient and eco-friendly adsorbent. In this research, Lantana Camara L. Stem biomass (LSB), a low-cost and useless material, was introduced as efficient biomass for divalent zinc biosorption from aqueous environments. For achieving optimal conditions in the zinc biosorption process, the experimental design was applied by the response surface methodology (RSM) based on a Box-Behnken design (BBD) model. Based on the comparison between the measured and predicted amounts, the values of R^2,〖 R〗_adj^2, and〖 R〗_pred^2 in the Zn(II) biosorption model were 0.9960, 0.9887 and 0.9441. The Zn(II) uptake in the experiments, BBD model-based (p-value of Lack-of-Fit term = 0.228 > 0.05), varied from 15.19% to 81.11%. The maximum analyte uptake at an LSB-to-Zn(II) ratio of 8:1, synthetic solution pH of 6.5 and residence time of 75 min was predicted at 97.12%. The maximum Z.R.% based on the validation test performed based on the optimal predicted conditions was also obtained at 94.65%, which is 2.5% different from the model's data amount, confirming the acceptable accuracy of the quadratic model. The LSB, in optimized conditions, as a low-cost biosorbent, can be a suitable candidate with acceptable potential for heavy metals biosorption from aquatic solutions.
In this research, pDNA-loaded chitosan/alginate-dextran sulfate nanoparticles with encapsulation ... more In this research, pDNA-loaded chitosan/alginate-dextran sulfate nanoparticles with encapsulation efficiency from 83.07 to 94.71% were prepared using a modified ionotropic gelation method. Encapsulated pDNA was tightly bound to polymers and protected from hydrolysis by a BamHI nuclease. All prepared nanoparticles were non-toxic for cells of the HEK298 line. Moreover, chitosan/alginate-dextran sulfate nanoparticles increased the proliferation of the cells in contrast to Lipofectamin TM, used as a positive control for transfection. The particles prepared were effective in the transfection of the pEGFP-N2 plasmid into the human cells. The high homogeneity of nanoparticles, reliable protection of the encapsulated DNA, and considerably high transfection efficiency along with the absence of toxicity make DNA-loaded chitosan/alginate-dextran particles suitable gene carriers in vitro and promising candidates for the design of delivery systems for gene therapy.
Environmental Progress & Sustainable Energy, 2022
Zn-based TMU-24 as a metal–organic framework (MOF), containing amide-functionalized pores was pre... more Zn-based TMU-24 as a metal–organic framework (MOF), containing amide-functionalized pores was prepared by a facile and efficient strategy. The obtained TMU-24 was illustrated to be efficient adsorbents for the eosin B removal from an aqueous solution. Due to their large surface area, stability, tunability, and porosity of the TMU-24, as well as π–π and hydrophobic interactions, hydrogen bonding formation between the dye and the MOF ligands, the fabricated sorbents showed fast adsorption kinetics (9 min) and high adsorption capacity. The effect of important factors such as pH, amount of adsorbent, contact time, and desorption conditions to achieve the optimal removal conditions was investigated. The optimum condition was pH = 6, absorbent dosage 4 mg and contact time 9 min. The adsorption results match with those of the Langmuir model and the pseudo-second-order kinetics, suggesting that eosin B was adsorbed to the adsorbent in monolayers due to its chemical affinity. The maximum adsorption capacity of TMU-24 for the removal of eosin B was 370.37 mg/g. Thermodynamic parameters suggested that the adsorption procedure is spontaneous and exothermic. TMU-24 exhibited excellent reusability (6 cycles) and had potential in the treatment of dyes polluted wastewater.
The effect of applied voltage on the structure of anodic films (including diameter of pores, thic... more The effect of applied voltage on the structure of anodic films (including diameter of pores, thickness of anodic film and pore density) was investigated. The morphology of the alumina film samples was characterized by scanning electron microscopy (SEM) using Clemex software. Meso-porous diameters ranged from 40 nm to 170 nm with thicknesses of more than 60 μm. The morphological shape was observed to be equiaxial with homogeneous pore distribution. Results can be summarized as follows: the higher the voltage, the bigger the pores and the thicker the anodic film. Increasing the voltage from 30 V up to 60 V, pore density reduced from 7.11 × 1014 to 4.8 × 1014.
In the offered study, a biosensor using a novel nano-matrix consisted of the human serum albumin ... more In the offered study, a biosensor using a novel nano-matrix consisted of the human serum albumin (HSA) and the Pd-doped CdTe quantum dots (Pd:CdTe) and sub-layer multiwall carbon nanotubes (MWCNT) was constructed. The biosensor was employed in rapid sensing of the chlorpyrifos (CPS) pesticide by a single frequency impedance (SFI) strategy. This biosensor response was based on the connections of the CPS to tryptophan residue located at position 214 of HSA content of matrix on the electrode. Biosensor response signals were recorded through impedance |Z| in different individual frequencies using the [Fe(CN)6]3−/4− probe as a connection index. The electron transfer resistance (Rct) of the biosensor was altered linearly by logarithm CPS concentration from 0.5 pM to 500 nM under the optimal conditions with a detection limit of 0.16 pM. The proposed presented biosensor exhibited high sensitivity, wide linear response, excellent reproducibility and stability. As a result, the proposed biosensor will be a promising candidate for screening trace amounts of CPS in the soil and water samples.
Journal of Chemical and Petroleum Engineering, 2019
In this study, a novel amine ionic liquid “Diethanolamine Lactic” [DEA][Lac] was synthesized. The... more In this study, a novel amine ionic liquid “Diethanolamine Lactic” [DEA][Lac] was synthesized. The replacement of halogenated ion fluid was used as a modified methodology for the preparation of diethanolamine based on lactic acid. The ionic liquid was characterized using the Fourier transform infrared (FTIR) spectra and the nuclear magnetic resonance (NMR) spectroscopy. The changes in bands' wavelengths and the peaks of the participating elements and process materials have been confirmed before and after synthesis based on the results of FTIR analysis which demonstrate the successful synthesis of diethanolamine lactic. The NMR analysis also clearly confirms the synthesis of diethanolamine lactic acid. Analysis results were shown in the successful synthesis of the [diethanolamine] [lactic].
Alginate beads, often used for controlled release of enzymes and drugs, are usually produced by s... more Alginate beads, often used for controlled release of enzymes and drugs, are usually produced by spraying sodium alginate liquid into a gelling agent using mechanical vibration nozzle or air jet. In this work an alternative method of electro-spray was employed to form droplets with desired size from a highly viscous sodium alginate solution using constant DC voltage. The droplets were then cured in a calcium chloride solution. The main objective was to produce mono-sized beads from such a highly viscous and non-Newtonian liquid (1000–5000 mPa s). The effects of nozzle diameter, flow rate and concentration of liquid on the size of the beads were investigated. Among the parameters studied, voltage had a pronounced effect on the size of beads as compared to flow rate, nozzle diameter and concentration of alginate liquid. The size of beads was reduced to a minimum value with increasing the voltage in the range of 0–10 kV. At the early stages of voltage increase (i.e. up to about 4 kV), the rate of size reduction was relatively low, while the dripping mode dominated. However, in the middle part of the range of applied voltage, where the rate of size reduction was high (i.e. about 4–7 kV), an unstable transition occurred between dripping and jetting. At the end part of the range (i.e. 7–10 kV) jet mode of spray was observed. Increasing the height of fall of the droplets was found to improve the sphericity of the beads, because of the increased time of flight for the droplets. This was especially identifiable at higher concentrations of the alginate liquid (i.e. 3 w/v%).
This paper focused on the study of the impact of ultrasonic waves on the intensification of Ni(II... more This paper focused on the study of the impact of ultrasonic waves on the intensification of Ni(II) removal from aqueous solution by adsorption onto Fe3O4 nanoparticles. Co-precipitation method was used to synthesis Fe3O4 magnetic nanoparticles (MNPs) and the average size of the nanoparticles was obtained about 19 nm by SEM. Two layouts including shaker and ultrasonic irradiation are examined. The effects of pH, adsorbent mass and initial concentration of Ni(II) on the removal efficiency of Ni(II) were investigated. The Ni(II) removal efficiency had the highest value at pH=9. Ni(II) removal from aqueous solution using ultrasonic need to lower contact time than the shaker at identical conditions. The highest removal efficiencies of Ni(II) were 83.3% and 85.5% with the contact times of 100 minutes and 60 minutes, respectively using the shaker and ultrasonic. Finally, Langmuir and Freundlich isotherms were employed to correlate sets of experimental adsorption isotherm data. The fitting ...
Electro-Hydrodynamics (EHD) spray is an important process in many engineering operations such as ... more Electro-Hydrodynamics (EHD) spray is an important process in many engineering operations such as ink-jet printing, spray drying and atomization (liquid jet in air), dispersion and emulsification (liquid jet in liquid). In this work the method of electrospray was employed to form droplets from a high viscous sodium-alginate solution using constant DC voltage. The droplets were cured in calcium chloride solution to produce solid beads. The main objective was to extend the knowledge of EHD spray to high viscous and non-Newtonian liquids (1000-5000 mPa.s). However, the effects of electric field strength, nozzle diameter, flow rate and concentration of liquid were specifically investigated on the size of the beads. Among the parameters studied, voltage had a pronounced effect on the size of the beads as compared to the flow rate, the nozzle diameter and the concentration of alginate liquid. The size of beads was reduced to a minimum value by increasing the voltage in the range of 0-10 kV...
The development of industrial factories has led to increasing the entry rate of non-degradable co... more The development of industrial factories has led to increasing the entry rate of non-degradable compounds into the environment. Natural sorbents have been used extensively to remove heavy metals under optimal conditions. In the present research, the lead-resistant bacteria (Delftia lacustris Strain-MS3) were isolated and identified and the ability of this novel strain to tolerate different concentrations of Pb+2 were evaluated. Kinetics, thermodynamic study and the optimal conditions for lead uptake by isolated strain were statistically described. Factors affecting optimum operational conditions of lead biosorption were analyzed using response surface methodology (RSM), based on the Box-Behnken design (BBD) model. The maximum lead removal measured at pH value of 6.6, bacterial biomass to lead ratio of 22:3, and residence time of 180 min. There was a significant difference between the growth rates of Strain-MS3 in different treatments. Results illustrate the high accuracy of the selec...
International Journal of Environmental Analytical Chemistry, 2022
Copper is an essential mineral that plays a vital role in the body, which in the event of an incr... more Copper is an essential mineral that plays a vital role in the body, which in the event of an increase it can be toxic to the body. The concentration of copper in the blood is very important. In the...
The MWCNTs/Fe3O4@TMU-21 as a novel electrochemical sandwich-type genosensor was fabricated to det... more The MWCNTs/Fe3O4@TMU-21 as a novel electrochemical sandwich-type genosensor was fabricated to detect the BRCA1 gene using the redox-cycling ferrocene functionalized reporter label probe (r-Fc-DNA). In the designed genosensor, the capture probe (cDNA) and r-Fc-DNA were used to detect the BRCA1 gene in sandwich-type genosensor, in which DNA sequences are well -hybridized with the BRCA1 gene (t-DNA). The cDNA was immobilized on the multiwall carbon nanotube and metal-organic framework with Fe3O4 nanoparticle core, which is the sensor platform. Target DNA was assayed by redox-recycling reporter probe (r-Fc-DNA) using the electro-catalytic activity of ferri/ferrocyanide, which results in significantly enhanced the oxidation peak current of r-Fc-DNA. The electrochemical redox cycling led to a high signal-to-noise ratio for gene assay. MWCNTs and Fe3O4@TMU-21 were applied to increase the platform conductivity and suitable binding of the recognition elements. This constructed genosensor pla...
Early diagnosis of tumors in the early stages of cancer allows for successful treatment and compl... more Early diagnosis of tumors in the early stages of cancer allows for successful treatment and complete recovery. Herein, a simple and novel electrochemical immunosensor for Human Epidermal growth factor Receptor 2 (HER2) detection was constructed employing electrocatalytic activity of the magnetic framework Fe3O4@ TMU-21. The surface of the Fe3O4 nanoparticles was functionalized with methacrylic acid (MAA) to facilitate the immobilization of TMU-21. The fabrication of the immunosensor platform is based on Fe3O4@ TMU-21, and multi-walled carbon nanotubes (MWCNT). Their electrocatalytic activity towards the reduction of hydrogen peroxide is applied to signal immunosensor. In the presence of HER2 biomarker (Ag), the amperometric current of the decline of H2O2 decreased, which could be concluded that the establishment of Ag –Ab complexes on the electrode, and consequence blocking the electron transfer of reduction of H2O2 at the electrode surface. The analytical performance results of the immunosensor indicate that a linear relationship between the logarithm of the HER2 concentrations and amperometric responses is within the range of 1.0 pg mL−1-100 ng mL−1 and the detection limit was 0.3 pg mL−1. Remarkably, the detection result of HER2 in real human serum samples by the proposed immunosensor is well in correlation with HER2 spiked to human blood serum samples, therefore applies to the recognition of HER2 biomarker in clinical monitoring of tumor marker.
Iranian Journal of Chemical Engineering (IJChE), 2009
Electro-Hydrodynamics (EHD) spray is an important process in many engineering operations such as ... more Electro-Hydrodynamics (EHD) spray is an important process in many engineering operations such as ink-jet printing, spray drying and atomization (liquid jet in air), dispersion and emulsification (liquid jet in liquid). In this work the method of electro-spray was employed to form droplets from a high viscous sodium-alginate solution using constant DC voltage. The droplets were cured in calcium chloride solution to produce solid beads. The main objective was to extend the knowledge of EHD spray to high viscous and non-Newtonian liquids (1000-5000 mPa.s). However, the effects of electric field strength, nozzle diameter, flow rate and concentration of liquid were specifically investigated on the size of the beads. Among the parameters studied, voltage had a pronounced effect on the size of the beads as compared to the flow rate, the nozzle diameter and the concentration of alginate liquid. The size of beads was reduced to a minimum value by increasing the voltage in the range of 0-10 k...
Spherical alginate-based hydrogels have been applied for environmental pollutants removal from aq... more Spherical alginate-based hydrogels have been applied for environmental pollutants removal from aqueous solutions. In this study, zinc alginate beads were prepared using an ionotropic gelation process. The wet hydrogels prepared in various operational conditions were studied to remove eosin-B dye. The effect of operational variables such as pH, initial concentration of eosin-B, contact time, dose of synthesized hydrogel beads, and environmental temperature on the eosin-B removal process was evaluated. The maximum dye removal efficiency was obtained by zinc alginate biosorbent at pH = 3, contact time of 40 min, and 35 mg of biosorbent dose. The maximum adsorption capacity of zinc alginate beads for eosin-B uptake was 53.48 mg/g of biosorbent. The mechanism, kinetics, isotherms, and thermodynamic studies of eosin-B sorption by zinc alginate hydrogels were investigated. Based on the results, the biosorption process follows pseudo-second-order kinetics. The experimental data of the biosorption process are matched with the Langmuir isotherm model, suggesting that monolayer adsorption plays a vital role in the analyte biosorption. According to the thermodynamic studies, the parameters ∆H° (+4679.12 J.mol-1), ∆G° (from -173.45 to -499.13 J.mol-1), and ΔS° (+16.284 J.mol-1.K-1) confirmed the endothermic, spontaneity and randomness of the biosorption process, respectively. This study shows that zinc alginate can be a suitable biosorbent for removing eosin-B from aqueous solutions.
Pearlescent pigments encompass a diverse range known for their ability to create an aesthetically... more Pearlescent pigments encompass a diverse range known for their ability to create an aesthetically pleasing optical appearance due to the facile alignment of numerous platelet-like particles. Consequently, they serve as an ideal choice for imparting robust, lustrous color effects in powder coatings. The synthesis of green pearlescent pigments was conducted in this study, utilizing mica (biotite) and mica-titania flakes coated with a copper oxide thin film. The process entailed the uniform formation of copper (II) chloride through the addition of sodium or potassium hydroxide in the existence of titania, mica, mica-alumina, ammonium molybdate, or mica-titania flakes. Biotite, denoted by the formula K(Mg/Fe)3(A1Si3O10)(OH)2, was selected as the supporting mica. The synthesized pigments were characterized using X-ray diffractometry (XRD) and X-ray fluorescence (XRF) techniques. Evaluations were conducted using polypropylene extrusion with the incorporated pigments, revealing acceptable chemical resistance and distinctive pearl luster effects. The XRD pattern of the Cu/mica pigment exhibited the presence of copper hydroxide covering the mica surface, confirming the formation of the green pearlescent pigments.
This study focused on the optimal production feasibility of distilled water from the concentrate ... more This study focused on the optimal production feasibility of distilled water from the concentrate of a reverse osmosis device using an evacuated tube collector basin solar still. The characteristic effect of the device's condenser on the produced freshwater volume was evaluated to improve the performance of the solar still, resulting in productivity enhancement. The direct contact of two faces of the cubic condenser with water vapor results in the doubling of the effective surface area. However, freshwater production was decreased by eight times compared to the case where only one face of the condenser is in contact with water vapor. This reduction was attributed to the shorter path taken by the condensed droplets. At the bottom of the evacuated tube collector, some building mineral pumice was used to prevent the formation of large water vapor bubbles. According to this methodology, the average daily production of distilled water from the concentrate of the reverse osmosis unit with a total dissolved solids of 15200 ppm was achieved at 4.58 ± 0.12 L.m − 2. The quality of produced distilled water revealed that solar desalination using an evacuated tube collector basin solar still can be a notable and economical solution for the concentrate treatment of reverse osmosis.
The solar still, in which saline or brackish water is converted into freshwater using renewable s... more The solar still, in which saline or brackish water is converted into freshwater using renewable solar energy, can be an effective solution to water scarcity. Inclined solar stills are well-known due to their high production yields compared to other types of solar stills. In this study, an inclined weir-type solar still was developed as a modified inclined solar still with an emphasis on the effect of structural parameters, such as weir height, distance between weirs, and distance between the absorber plate and the condenser cover, on distilled water production. The optimal values of structural parameters for the maximum freshwater production were specified by the response surface methodology and a quadratic model derived from a Box-Behnken design model. The results showed that the operational variables of the inclined weir-type solar still system significantly impacted the water production volume. Using a weir height of 2 cm, a distance of 3.5 cm between weirs, and a distance of 15 cm between the absorber plate and the condenser cover, the maximum water production volume was achieved at 6.474 kg m − 2. day − 1. The selected model had R 2 , R 2 adj and R 2 pred values of 0.9978, 0.9939, and 0.9756, respectively. The maximum and minimum volumes of the produced water were obtained as 6.64125 and 1.79792 kg m − 2. day 1 , based on the multiple response prediction of the variables. According to the validation tests, the model's accuracy in prediction of the maximum amount of freshwater production was notable (97.49%).
Biomass extracted from different plant parts can play a role as a cheap, efficient and eco-friend... more Biomass extracted from different plant parts can play a role as a cheap, efficient and eco-friendly adsorbent. In this research, Lantana Camara L. Stem biomass (LSB), a low-cost and useless material, was introduced as efficient biomass for divalent zinc biosorption from aqueous environments. For achieving optimal conditions in the zinc biosorption process, the experimental design was applied by the response surface methodology (RSM) based on a Box-Behnken design (BBD) model. Based on the comparison between the measured and predicted amounts, the values of R^2,〖 R〗_adj^2, and〖 R〗_pred^2 in the Zn(II) biosorption model were 0.9960, 0.9887 and 0.9441. The Zn(II) uptake in the experiments, BBD model-based (p-value of Lack-of-Fit term = 0.228 > 0.05), varied from 15.19% to 81.11%. The maximum analyte uptake at an LSB-to-Zn(II) ratio of 8:1, synthetic solution pH of 6.5 and residence time of 75 min was predicted at 97.12%. The maximum Z.R.% based on the validation test performed based on the optimal predicted conditions was also obtained at 94.65%, which is 2.5% different from the model's data amount, confirming the acceptable accuracy of the quadratic model. The LSB, in optimized conditions, as a low-cost biosorbent, can be a suitable candidate with acceptable potential for heavy metals biosorption from aquatic solutions.
In this research, pDNA-loaded chitosan/alginate-dextran sulfate nanoparticles with encapsulation ... more In this research, pDNA-loaded chitosan/alginate-dextran sulfate nanoparticles with encapsulation efficiency from 83.07 to 94.71% were prepared using a modified ionotropic gelation method. Encapsulated pDNA was tightly bound to polymers and protected from hydrolysis by a BamHI nuclease. All prepared nanoparticles were non-toxic for cells of the HEK298 line. Moreover, chitosan/alginate-dextran sulfate nanoparticles increased the proliferation of the cells in contrast to Lipofectamin TM, used as a positive control for transfection. The particles prepared were effective in the transfection of the pEGFP-N2 plasmid into the human cells. The high homogeneity of nanoparticles, reliable protection of the encapsulated DNA, and considerably high transfection efficiency along with the absence of toxicity make DNA-loaded chitosan/alginate-dextran particles suitable gene carriers in vitro and promising candidates for the design of delivery systems for gene therapy.
Environmental Progress & Sustainable Energy, 2022
Zn-based TMU-24 as a metal–organic framework (MOF), containing amide-functionalized pores was pre... more Zn-based TMU-24 as a metal–organic framework (MOF), containing amide-functionalized pores was prepared by a facile and efficient strategy. The obtained TMU-24 was illustrated to be efficient adsorbents for the eosin B removal from an aqueous solution. Due to their large surface area, stability, tunability, and porosity of the TMU-24, as well as π–π and hydrophobic interactions, hydrogen bonding formation between the dye and the MOF ligands, the fabricated sorbents showed fast adsorption kinetics (9 min) and high adsorption capacity. The effect of important factors such as pH, amount of adsorbent, contact time, and desorption conditions to achieve the optimal removal conditions was investigated. The optimum condition was pH = 6, absorbent dosage 4 mg and contact time 9 min. The adsorption results match with those of the Langmuir model and the pseudo-second-order kinetics, suggesting that eosin B was adsorbed to the adsorbent in monolayers due to its chemical affinity. The maximum adsorption capacity of TMU-24 for the removal of eosin B was 370.37 mg/g. Thermodynamic parameters suggested that the adsorption procedure is spontaneous and exothermic. TMU-24 exhibited excellent reusability (6 cycles) and had potential in the treatment of dyes polluted wastewater.
The effect of applied voltage on the structure of anodic films (including diameter of pores, thic... more The effect of applied voltage on the structure of anodic films (including diameter of pores, thickness of anodic film and pore density) was investigated. The morphology of the alumina film samples was characterized by scanning electron microscopy (SEM) using Clemex software. Meso-porous diameters ranged from 40 nm to 170 nm with thicknesses of more than 60 μm. The morphological shape was observed to be equiaxial with homogeneous pore distribution. Results can be summarized as follows: the higher the voltage, the bigger the pores and the thicker the anodic film. Increasing the voltage from 30 V up to 60 V, pore density reduced from 7.11 × 1014 to 4.8 × 1014.
In the offered study, a biosensor using a novel nano-matrix consisted of the human serum albumin ... more In the offered study, a biosensor using a novel nano-matrix consisted of the human serum albumin (HSA) and the Pd-doped CdTe quantum dots (Pd:CdTe) and sub-layer multiwall carbon nanotubes (MWCNT) was constructed. The biosensor was employed in rapid sensing of the chlorpyrifos (CPS) pesticide by a single frequency impedance (SFI) strategy. This biosensor response was based on the connections of the CPS to tryptophan residue located at position 214 of HSA content of matrix on the electrode. Biosensor response signals were recorded through impedance |Z| in different individual frequencies using the [Fe(CN)6]3−/4− probe as a connection index. The electron transfer resistance (Rct) of the biosensor was altered linearly by logarithm CPS concentration from 0.5 pM to 500 nM under the optimal conditions with a detection limit of 0.16 pM. The proposed presented biosensor exhibited high sensitivity, wide linear response, excellent reproducibility and stability. As a result, the proposed biosensor will be a promising candidate for screening trace amounts of CPS in the soil and water samples.
Journal of Chemical and Petroleum Engineering, 2019
In this study, a novel amine ionic liquid “Diethanolamine Lactic” [DEA][Lac] was synthesized. The... more In this study, a novel amine ionic liquid “Diethanolamine Lactic” [DEA][Lac] was synthesized. The replacement of halogenated ion fluid was used as a modified methodology for the preparation of diethanolamine based on lactic acid. The ionic liquid was characterized using the Fourier transform infrared (FTIR) spectra and the nuclear magnetic resonance (NMR) spectroscopy. The changes in bands' wavelengths and the peaks of the participating elements and process materials have been confirmed before and after synthesis based on the results of FTIR analysis which demonstrate the successful synthesis of diethanolamine lactic. The NMR analysis also clearly confirms the synthesis of diethanolamine lactic acid. Analysis results were shown in the successful synthesis of the [diethanolamine] [lactic].
Alginate beads, often used for controlled release of enzymes and drugs, are usually produced by s... more Alginate beads, often used for controlled release of enzymes and drugs, are usually produced by spraying sodium alginate liquid into a gelling agent using mechanical vibration nozzle or air jet. In this work an alternative method of electro-spray was employed to form droplets with desired size from a highly viscous sodium alginate solution using constant DC voltage. The droplets were then cured in a calcium chloride solution. The main objective was to produce mono-sized beads from such a highly viscous and non-Newtonian liquid (1000–5000 mPa s). The effects of nozzle diameter, flow rate and concentration of liquid on the size of the beads were investigated. Among the parameters studied, voltage had a pronounced effect on the size of beads as compared to flow rate, nozzle diameter and concentration of alginate liquid. The size of beads was reduced to a minimum value with increasing the voltage in the range of 0–10 kV. At the early stages of voltage increase (i.e. up to about 4 kV), the rate of size reduction was relatively low, while the dripping mode dominated. However, in the middle part of the range of applied voltage, where the rate of size reduction was high (i.e. about 4–7 kV), an unstable transition occurred between dripping and jetting. At the end part of the range (i.e. 7–10 kV) jet mode of spray was observed. Increasing the height of fall of the droplets was found to improve the sphericity of the beads, because of the increased time of flight for the droplets. This was especially identifiable at higher concentrations of the alginate liquid (i.e. 3 w/v%).
This paper focused on the study of the impact of ultrasonic waves on the intensification of Ni(II... more This paper focused on the study of the impact of ultrasonic waves on the intensification of Ni(II) removal from aqueous solution by adsorption onto Fe3O4 nanoparticles. Co-precipitation method was used to synthesis Fe3O4 magnetic nanoparticles (MNPs) and the average size of the nanoparticles was obtained about 19 nm by SEM. Two layouts including shaker and ultrasonic irradiation are examined. The effects of pH, adsorbent mass and initial concentration of Ni(II) on the removal efficiency of Ni(II) were investigated. The Ni(II) removal efficiency had the highest value at pH=9. Ni(II) removal from aqueous solution using ultrasonic need to lower contact time than the shaker at identical conditions. The highest removal efficiencies of Ni(II) were 83.3% and 85.5% with the contact times of 100 minutes and 60 minutes, respectively using the shaker and ultrasonic. Finally, Langmuir and Freundlich isotherms were employed to correlate sets of experimental adsorption isotherm data. The fitting ...
Electro-Hydrodynamics (EHD) spray is an important process in many engineering operations such as ... more Electro-Hydrodynamics (EHD) spray is an important process in many engineering operations such as ink-jet printing, spray drying and atomization (liquid jet in air), dispersion and emulsification (liquid jet in liquid). In this work the method of electrospray was employed to form droplets from a high viscous sodium-alginate solution using constant DC voltage. The droplets were cured in calcium chloride solution to produce solid beads. The main objective was to extend the knowledge of EHD spray to high viscous and non-Newtonian liquids (1000-5000 mPa.s). However, the effects of electric field strength, nozzle diameter, flow rate and concentration of liquid were specifically investigated on the size of the beads. Among the parameters studied, voltage had a pronounced effect on the size of the beads as compared to the flow rate, the nozzle diameter and the concentration of alginate liquid. The size of beads was reduced to a minimum value by increasing the voltage in the range of 0-10 kV...
The development of industrial factories has led to increasing the entry rate of non-degradable co... more The development of industrial factories has led to increasing the entry rate of non-degradable compounds into the environment. Natural sorbents have been used extensively to remove heavy metals under optimal conditions. In the present research, the lead-resistant bacteria (Delftia lacustris Strain-MS3) were isolated and identified and the ability of this novel strain to tolerate different concentrations of Pb+2 were evaluated. Kinetics, thermodynamic study and the optimal conditions for lead uptake by isolated strain were statistically described. Factors affecting optimum operational conditions of lead biosorption were analyzed using response surface methodology (RSM), based on the Box-Behnken design (BBD) model. The maximum lead removal measured at pH value of 6.6, bacterial biomass to lead ratio of 22:3, and residence time of 180 min. There was a significant difference between the growth rates of Strain-MS3 in different treatments. Results illustrate the high accuracy of the selec...
International Journal of Environmental Analytical Chemistry, 2022
Copper is an essential mineral that plays a vital role in the body, which in the event of an incr... more Copper is an essential mineral that plays a vital role in the body, which in the event of an increase it can be toxic to the body. The concentration of copper in the blood is very important. In the...
The MWCNTs/Fe3O4@TMU-21 as a novel electrochemical sandwich-type genosensor was fabricated to det... more The MWCNTs/Fe3O4@TMU-21 as a novel electrochemical sandwich-type genosensor was fabricated to detect the BRCA1 gene using the redox-cycling ferrocene functionalized reporter label probe (r-Fc-DNA). In the designed genosensor, the capture probe (cDNA) and r-Fc-DNA were used to detect the BRCA1 gene in sandwich-type genosensor, in which DNA sequences are well -hybridized with the BRCA1 gene (t-DNA). The cDNA was immobilized on the multiwall carbon nanotube and metal-organic framework with Fe3O4 nanoparticle core, which is the sensor platform. Target DNA was assayed by redox-recycling reporter probe (r-Fc-DNA) using the electro-catalytic activity of ferri/ferrocyanide, which results in significantly enhanced the oxidation peak current of r-Fc-DNA. The electrochemical redox cycling led to a high signal-to-noise ratio for gene assay. MWCNTs and Fe3O4@TMU-21 were applied to increase the platform conductivity and suitable binding of the recognition elements. This constructed genosensor pla...
Early diagnosis of tumors in the early stages of cancer allows for successful treatment and compl... more Early diagnosis of tumors in the early stages of cancer allows for successful treatment and complete recovery. Herein, a simple and novel electrochemical immunosensor for Human Epidermal growth factor Receptor 2 (HER2) detection was constructed employing electrocatalytic activity of the magnetic framework Fe3O4@ TMU-21. The surface of the Fe3O4 nanoparticles was functionalized with methacrylic acid (MAA) to facilitate the immobilization of TMU-21. The fabrication of the immunosensor platform is based on Fe3O4@ TMU-21, and multi-walled carbon nanotubes (MWCNT). Their electrocatalytic activity towards the reduction of hydrogen peroxide is applied to signal immunosensor. In the presence of HER2 biomarker (Ag), the amperometric current of the decline of H2O2 decreased, which could be concluded that the establishment of Ag –Ab complexes on the electrode, and consequence blocking the electron transfer of reduction of H2O2 at the electrode surface. The analytical performance results of the immunosensor indicate that a linear relationship between the logarithm of the HER2 concentrations and amperometric responses is within the range of 1.0 pg mL−1-100 ng mL−1 and the detection limit was 0.3 pg mL−1. Remarkably, the detection result of HER2 in real human serum samples by the proposed immunosensor is well in correlation with HER2 spiked to human blood serum samples, therefore applies to the recognition of HER2 biomarker in clinical monitoring of tumor marker.
Iranian Journal of Chemical Engineering (IJChE), 2009
Electro-Hydrodynamics (EHD) spray is an important process in many engineering operations such as ... more Electro-Hydrodynamics (EHD) spray is an important process in many engineering operations such as ink-jet printing, spray drying and atomization (liquid jet in air), dispersion and emulsification (liquid jet in liquid). In this work the method of electro-spray was employed to form droplets from a high viscous sodium-alginate solution using constant DC voltage. The droplets were cured in calcium chloride solution to produce solid beads. The main objective was to extend the knowledge of EHD spray to high viscous and non-Newtonian liquids (1000-5000 mPa.s). However, the effects of electric field strength, nozzle diameter, flow rate and concentration of liquid were specifically investigated on the size of the beads. Among the parameters studied, voltage had a pronounced effect on the size of the beads as compared to the flow rate, the nozzle diameter and the concentration of alginate liquid. The size of beads was reduced to a minimum value by increasing the voltage in the range of 0-10 k...
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