<p>(<b>A</b>) Representative samples from a control donor and a <i>T. cru... more <p>(<b>A</b>) Representative samples from a control donor and a <i>T. cruzi</i>-infected individual. PBMC were stained with Abs to CD19, CD27, IgD, IgM and IgG, and analyzed by flow cytometry. Numbers correspond to the percentage of cells within the gate. (<b>B</b>) Box and whiskers (min to max) show the percentage of cells expressing the indicated markers for control (n = 8) and infected individuals (n = 18). Groups were compared by the Mann-Whitney nonparametric test or U-test and statistical significance (p values) is indicated.</p
In previous studies we have shown that human chronic T. cruzi infection is associated with abnorm... more In previous studies we have shown that human chronic T. cruzi infection is associated with abnormalities of CD8+ and CD4+ T cell compartments, probably as a consequence of chronic antigenic stimulation by persisting parasites. In this study, we measured the levels of circulating B cell subsets in 25 patients chronically infected with T. cruzi and 19 non-infected controls (mean age±SD=41.0±12.2 and 34.9±10.6 y respectively). The results showed that T. cruzi-infected patients had higher percentages of CD19+CD27-IgD+ (naïve; mean%±SD=54.1±16.1) and lower percentages of CD19+CD27+ (Ag-experienced; mean%±SD=35.8±16.5) B lymphocytes compared to controls (mean%±SD=33.8±15.8 and 55.7±18.0, respectively, p&lt;0.05), with similar levels of CD69 or CD86 activation markers. The observed memory B cell reduction comprised switched (IgD-) and no-switched (IgD+) subsets (mean%±SD=17.9±11.2 and 17.9±12.6, respectively) in relation to controls (mean%±SD=30.9±17.8 and 24.8±16.4, respectively; p&lt;0.05). The proportion of CD19+CD27+CD138+ plasma cells in patients was lower than in the controls (mean%±SD=5.0±3.6 and 26.4±27.1 respectively; p&lt;0.05), with no differences in CD19+CD10+ cell levels (immature; mean%±SD=5.6±4.7 and 4.3±3.3, respectively). The accumulation of naïve and loss of memory B cell and plasma cell subsets in chronic Chagas disease patients indicate that T. cruzi infection affects the phenotypic profile of the peripheral blood B cell-compartment. Support: FONCyT PICT 2007 N°1732
<p>The levels of sCD27 were measured by ELISA in individuals with different degrees of card... more <p>The levels of sCD27 were measured by ELISA in individuals with different degrees of cardiac dysfunction and controls. Bars represent the mean and SD for each group. Statistical significance was determined with One-way ANOVA followed by Bonferroni’s post-test. *p<0.05. <b><sup>ƒ</sup></b> N, normal and A, abnormal.</p
Trypanosoma cruzi was shown to induce the loss of parasite-specific IgG-producing B cells during ... more Trypanosoma cruzi was shown to induce the loss of parasite-specific IgG-producing B cells during the acute infection of mice, which was proposed to hamper B cell responses and favor parasite replication and chronicity (Zuñiga et al. 2002). Herein, we measured the levels of circulating memory and MZ-like B cells in 12 individuals chronically infected with T. cruzi and 9 healthy controls based on the expression of CD19, IgM IgD, IgG, and CD27. T. cruzi-infected individuals had significantly lower percentages of IgM+IgD-CD27+ (IgM-only), IgM-IgD-CD27+ (isotype-switched) and IgG+IgD-CD27+ memory B cells compared to controls (p<0.05). Also, the percentage of IgM+IgD+CD27+ (MZ-like) B cells was reduced in infected individuals while the proportion of IgG+IgD-CD27- memory B cells was increased (p<0.05). The levels of memory B cells were further analyzed according to ECG findings in infected individuals (asymptomatic [ASY] and chronic Chagas heart disease [CHD]). IgG+IgD-CD27+, IgM+IgD...
In previous studies we have shown that human chronic T. cruzi infection is associated with abnorm... more In previous studies we have shown that human chronic T. cruzi infection is associated with abnormalities of CD8+ and CD4+ T cell compartments, probably as a consequence of chronic antigenic stimulation by persisting parasites. In this study, we measured the levels of circulating B cell subsets in 25 patients chronically infected with T. cruzi and 19 non-infected controls (mean age±SD=41.0±12.2 and 34.9±10.6 y respectively). The results showed that T. cruzi-infected patients had higher percentages of CD19+CD27-IgD+ (naïve; mean%±SD=54.1±16.1) and lower percentages of CD19+CD27+ (Ag-experienced; mean%±SD=35.8±16.5) B lymphocytes compared to controls (mean%±SD=33.8±15.8 and 55.7±18.0, respectively, p<0.05), with similar levels of CD69 or CD86 activation markers. The observed memory B cell reduction comprised switched (IgD-) and no-switched (IgD+) subsets (mean%±SD=17.9±11.2 and 17.9±12.6, respectively) in relation to controls (mean%±SD=30.9±17.8 and 24.8±16.4, respectively; p<0....
<p>(<b>A</b>) Representative samples from a control donor and a <i>T. cru... more <p>(<b>A</b>) Representative samples from a control donor and a <i>T. cruzi</i>-infected individual. PBMC were stained with Abs to CD19, CD27, IgD, IgM and IgG, and analyzed by flow cytometry. Numbers correspond to the percentage of cells within the gate. (<b>B</b>) Box and whiskers (min to max) show the percentage of cells expressing the indicated markers for control (n = 8) and infected individuals (n = 18). Groups were compared by the Mann-Whitney nonparametric test or U-test and statistical significance (p values) is indicated.</p
In previous studies we have shown that human chronic T. cruzi infection is associated with abnorm... more In previous studies we have shown that human chronic T. cruzi infection is associated with abnormalities of CD8+ and CD4+ T cell compartments, probably as a consequence of chronic antigenic stimulation by persisting parasites. In this study, we measured the levels of circulating B cell subsets in 25 patients chronically infected with T. cruzi and 19 non-infected controls (mean age±SD=41.0±12.2 and 34.9±10.6 y respectively). The results showed that T. cruzi-infected patients had higher percentages of CD19+CD27-IgD+ (naïve; mean%±SD=54.1±16.1) and lower percentages of CD19+CD27+ (Ag-experienced; mean%±SD=35.8±16.5) B lymphocytes compared to controls (mean%±SD=33.8±15.8 and 55.7±18.0, respectively, p&lt;0.05), with similar levels of CD69 or CD86 activation markers. The observed memory B cell reduction comprised switched (IgD-) and no-switched (IgD+) subsets (mean%±SD=17.9±11.2 and 17.9±12.6, respectively) in relation to controls (mean%±SD=30.9±17.8 and 24.8±16.4, respectively; p&lt;0.05). The proportion of CD19+CD27+CD138+ plasma cells in patients was lower than in the controls (mean%±SD=5.0±3.6 and 26.4±27.1 respectively; p&lt;0.05), with no differences in CD19+CD10+ cell levels (immature; mean%±SD=5.6±4.7 and 4.3±3.3, respectively). The accumulation of naïve and loss of memory B cell and plasma cell subsets in chronic Chagas disease patients indicate that T. cruzi infection affects the phenotypic profile of the peripheral blood B cell-compartment. Support: FONCyT PICT 2007 N°1732
<p>The levels of sCD27 were measured by ELISA in individuals with different degrees of card... more <p>The levels of sCD27 were measured by ELISA in individuals with different degrees of cardiac dysfunction and controls. Bars represent the mean and SD for each group. Statistical significance was determined with One-way ANOVA followed by Bonferroni’s post-test. *p<0.05. <b><sup>ƒ</sup></b> N, normal and A, abnormal.</p
Trypanosoma cruzi was shown to induce the loss of parasite-specific IgG-producing B cells during ... more Trypanosoma cruzi was shown to induce the loss of parasite-specific IgG-producing B cells during the acute infection of mice, which was proposed to hamper B cell responses and favor parasite replication and chronicity (Zuñiga et al. 2002). Herein, we measured the levels of circulating memory and MZ-like B cells in 12 individuals chronically infected with T. cruzi and 9 healthy controls based on the expression of CD19, IgM IgD, IgG, and CD27. T. cruzi-infected individuals had significantly lower percentages of IgM+IgD-CD27+ (IgM-only), IgM-IgD-CD27+ (isotype-switched) and IgG+IgD-CD27+ memory B cells compared to controls (p<0.05). Also, the percentage of IgM+IgD+CD27+ (MZ-like) B cells was reduced in infected individuals while the proportion of IgG+IgD-CD27- memory B cells was increased (p<0.05). The levels of memory B cells were further analyzed according to ECG findings in infected individuals (asymptomatic [ASY] and chronic Chagas heart disease [CHD]). IgG+IgD-CD27+, IgM+IgD...
In previous studies we have shown that human chronic T. cruzi infection is associated with abnorm... more In previous studies we have shown that human chronic T. cruzi infection is associated with abnormalities of CD8+ and CD4+ T cell compartments, probably as a consequence of chronic antigenic stimulation by persisting parasites. In this study, we measured the levels of circulating B cell subsets in 25 patients chronically infected with T. cruzi and 19 non-infected controls (mean age±SD=41.0±12.2 and 34.9±10.6 y respectively). The results showed that T. cruzi-infected patients had higher percentages of CD19+CD27-IgD+ (naïve; mean%±SD=54.1±16.1) and lower percentages of CD19+CD27+ (Ag-experienced; mean%±SD=35.8±16.5) B lymphocytes compared to controls (mean%±SD=33.8±15.8 and 55.7±18.0, respectively, p<0.05), with similar levels of CD69 or CD86 activation markers. The observed memory B cell reduction comprised switched (IgD-) and no-switched (IgD+) subsets (mean%±SD=17.9±11.2 and 17.9±12.6, respectively) in relation to controls (mean%±SD=30.9±17.8 and 24.8±16.4, respectively; p<0....
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