Papers by Matthieu Chabannes
Banana streak viruses (BSV) infect bananas and plantains (Musa spp.) worldwide. BSV became a majo... more Banana streak viruses (BSV) infect bananas and plantains (Musa spp.) worldwide. BSV became a major constraint for banana breeding programmes and germplasm exchanges since the discovery a couple of decade ago of the presence of infectious BSV integrations (eBSV) in the genome of Musa balbisiana (B genome) which can under certain stress conditions released spontaneously fonctional and infectious BSV. To date, BSV is known to only infect banana but for several reasons working with banana is very time consuming and not an easy task (space needed, long life cycle, no mutant database available, transient and stable transformations are difficult and laborious...). To overcome most of those problems and to both understand the mechanisms underlying the regulation of eBSV activation as well as eBSV non-activation and gain knowledge in the BSV biology, we tried to establish infection of Arabidopsis thaliana (At). We stably transformed two different ecotypes of At via agrobacterium using a more...
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Banana streak viruses (BSV) infect bananas and plantains (Musa spp.) worldwide. BSV became a majo... more Banana streak viruses (BSV) infect bananas and plantains (Musa spp.) worldwide. BSV became a major constraint for banana breeding programmes and germplasm exchanges since the discovery a couple of decade ago of the presence of infectious BSV integrations (eBSV) in the genome of Musa balbisiana (B genome) which can under certain stress conditions released spontaneously fonctional and infectious BSV. To date, BSV is known to only infect banana but for several reasons working with banana is very time consuming and not an easy task (space needed, long life cycle, no mutant database available, transient and stable transformations are difficult and laborious...). To overcome most of those problems and to both understand the mechanisms underlying the regulation of eBSV activation as well as eBSV non-activation and gain knowledge in the BSV biology, we tried to establish infection of Arabidopsis thaliana (At). We stably transformed two different ecotypes of At via agrobacterium using a more...
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Xanthomonas campestris pv. campestris is a vascular phytopathogenic bacterium which is the causal... more Xanthomonas campestris pv. campestris is a vascular phytopathogenic bacterium which is the causal agent of black rot of Brassicaceae such as cabbage or the model plant Arabidopsis thaliana. Its virulence relies on a type III secretion system which injects virulence proteins (also called effectors) directly inside the plant cell. The xopAC gene encodes a type III effector which is Xanthomonas campestris-specific and responsible for the avirulence of Xcc on the Columbia-0 ecotype of A. thaliana when Xcc is directly inoculated in the leaf central vein. The XopAC protein has a N-terminal LRR domain (Leucine Rich Repeat) and a C-terminal Fic domain (Filamentation Induced by CAMP). The LRR is probably involved in an interaction with a plant ligand. The Fic domain is probably involved in the post-translational modification of protein targets by adenylylation (adding of AMP moiety) on a threonine or tyrosine residue. We have shown that both domains are essential for the avirulence function ...
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Banana cultivars (including plantain) are derived from natural hybridization between wild diploid... more Banana cultivars (including plantain) are derived from natural hybridization between wild diploid Musa species and subspecies. The main species involved are Musa acuminata (A genome) that is encountered in all banana cultivars, and M. balbisiana (B genome) in many of them. The usual strategy to produce improved AAB plantain like hybrids exploits residual fertility of existing triploids cultivars and relies on two successive crosses. The first cross involves a plantain (AAB) and a highly fertile diploid (AA) banana to produce offsprings from which a tetraploid is selected. In a second step, this selected tetraploid is crossed with another diploid accession to produce triploid offspring from which improved hybrids may be selected. However, plantain breeding was hampered since the mid-1990s by the discovery in the B genome of infectious endogenous sequences of banana streak viruses (eBSVs), which in interspecific genome context, are able to produce BSV particles causing banana streak d...
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Banana streak virus (BSV) belongs to the genus Badnavirus of plant pararetroviruses (family Cauli... more Banana streak virus (BSV) belongs to the genus Badnavirus of plant pararetroviruses (family Caulimoviridae) that replicate by reverse transcription of pregenomic (pg)RNA and encapsidate circular non-covalently closed double stranded genomic DNA of ca. 7.4-8 kb to form episomal viruses. A complex of BSV species cause the streak disease transmitted by mealybugs in bananas and plantains worldwide. Gaining the knowledge on transcriptional regulation of episomal BSV is an important step forward in developing efficient strategies to fight against BSV. Schenk et al., 2001 have shown that the 1.3 and 2.1 kb fragments of the intergenic region of BSV Cavendish (BSCAV) and BSV Mysore (BSMYV) species, respectively, exhibited a promoter activity in a wide range of plant species including monocots, dicots, gymnosperm and fern. In 2005, Remans et al. have identified four elements in the intergenic region that are important for the activity of the BSCAV promoter. Here we mapped the pgRNA promoter o...
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Banana streak virus (BSV), the causative agent of banana streak disease, is a plant pararetroviru... more Banana streak virus (BSV), the causative agent of banana streak disease, is a plant pararetrovirus belonging to the family Caulimoviridae, genus Badnavirus. The genome of BSV is a circular double ? stranded DNA of 7.4 kbp made of three ORFs and like other pararetroviruses replicates via reverse transcription of viral pregenomic RNA (Lockhart, 1990). In the mid-ninetys Ndowora et al. (1999), revealed the presence of multiple integrations of Banana streak virus in the genome of banana (Musa sp.) whereas this badnavirus does not require integration for its replication. Some endogenous BSV (eBSV) sequences in the Musa balbisiana genome are infectious by releasing a functional viral genome following stresses such as those existing in in vitro culture and interspecific crosses context. Seedy M. balbisiana diploid genotypes (BB) such as Pisang Klutuk Wulung (PKW) harbour such infectious eBSV belonging to three widespread species of BSV (Goldfinger -BSGFV, Imove - BSImV and Obino l'Ewai...
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The Plant Journal, 2001
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Nature, 2012
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Journal of General Virology
The main edible and cultivated banana varieties are intra- and interspecific hybrids of the two m... more The main edible and cultivated banana varieties are intra- and interspecific hybrids of the two main Musa species, Musa acuminata and Musa balbisiana, having diploid genomes denoted A and B, respectively. The B genome naturally hosts sequences of banana streak virus (BSV) named endogenous BSV (eBSV). Upon stress, eBSVs are identified as the origin of BSV infection for at least three BSV species, causing banana streak disease. For each of the three species, BSV and eBSV share >99.9 % sequence identity, complicating PCR-based diagnosis of viral infection in the B genome-containing bananas. Here, we designed a quantitative PCR-based method to only quantify episomal BSV particles produced, overcoming the limitation of eBSV also being detected by qPCR by using it as a ‘calibrator’. However, our results revealed unexpected variation of eBSV amplification in calibrator plants composed of a clonal population of 53 replicating virus-free banana hybrids with the same AAB genotype. Our in-d...
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Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot on Brassicae and cau... more Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot on Brassicae and causes disease on crop plants such as cabbage or on the model plant Arabidopsis. The xopAC gene encodes a type III effector which is responsible for avirulence on Arabidopsis ecotype Col-0 exclusively when Xcc is inoculated in the leaf vasculature (1). PCRs and dot-blot hybridizations performed on a large collection of plant pathogenic bacteria revealed that avrAC is specific to Xc. The analysis of more than 50 Xcc strains reveals that avrAC displays a very low allelic diversity and belongs to the Xcc variable effectome. Moreover, the presence of avrAC is tightly correlated with an increase in Xcc aggressiveness on susceptible Arabidopsis. This correlation was experimentally confirmed by reverse genetic in several Xcc strains. In addition, xopAC is responsive for the appearance of necrotic lesions on nonhost pepper plants. We show that the "Leucine-Rich Repeat" (LRR) and "Fil...
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Infections of banana and plantain by banana streak viruses (BSV) can occur in the absence of vect... more Infections of banana and plantain by banana streak viruses (BSV) can occur in the absence of vector-mediated transmission, through the activation of infectious endogenous BSV sequences (eBSVs). Such infectious eBSVs are present in the genome of Musa balbisiana spp, which are important progenitors for breeding improved banana varieties. Once activated by biotic or abiotic stresses, these viral integrants cause spontaneous infection in both natural and synthetic interspecific hybrids harbouring the M. balbisiana (B) genome. Therefore, the presence of infectious eBSVs within B genomes is the main constraint for breeding banana and plantain interspecific hybrids and for exchanging Musa germplasm. The sequence and organization of eBSVs in the diploid M. balbisiana genitor Pisang Klutuk Wulung (PKW) was elucidated for BSV species Obino l'EwaI (BSOLV), Goldfinger (BSGFV) and ImovO (BSIMV). This work showed that integration of BSGFV and BSOLV is di-allelic, with one infectious and one n...
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Breeding new interspecific banana hybrid varieties with pests and disease resistance characters i... more Breeding new interspecific banana hybrid varieties with pests and disease resistance characters is instrumental for the development of pesticide-free agroecological banana farming systems. Such breeding relies on the combined use of Musa acuminata and M. balbisiana parents. Unfortunately, infectious alleles of endogenous Banana streak virus (eBSV) sequences are present in the genome of M. balbisiana genitors. Upon activation by biotic and abiotic stresses, these infectious eBSVs lead to spontaneous infections by several species of Banana streak virus (BSV) in interspecific hybrids harbouring both M. acuminata and M. balbisiana genomes. In this work, we show that M. balbisiana diploid genitors available for breeding host at least one infectious eBSV. We also show how we segregated infectious and non-infectious eBSV alleles in seedy M. balbisiana diploids through self-pollination or chromosome doubling of haploid lines. We report on the successful breeding of M. balbisiana diploid gen...
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The genome of banana (Musa sp.) harbours multiple integrations of several species of Banana strea... more The genome of banana (Musa sp.) harbours multiple integrations of several species of Banana streak virus (BSV), certainly resulting from illegitimate recombination between host and viral DNA. Surprisingly, this badnavirus does not require integration for its replication. Some integrations, only existing in the Musa balbisiana genome (denoted B), are infectious by releasing a functional viral genome following stresses such as in vitro culture and interspecific crosses. To date, four widespread species of BSV (Goldfinger -BSGFV, Imove - BSImV, Mysore - BSMysV and Obino l'Ewai - BSOLV) have been reported as integrated into the B genome with three of them as infectious (eBSGFV, eBSImV and eBSOLV). In order to study BSV expression from such viral integrants and to retrace their evolutionary story, a full genomic and genetic characterisation of BSV integrants (eBSV) was undergone including cytogenetic localization on chromosomes. Very low copies of integrations were recorded for each ...
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The genome of banana (Musa sp.) harbours multiple integrations of Banana streak virus (e BSV), wh... more The genome of banana (Musa sp.) harbours multiple integrations of Banana streak virus (e BSV), whereas this badnavirus does not require integration for the r eplication of its ds DNA genome. Some endogenous BSV sequences (eBSV), only existing in the Musa balbisiana genome, are infectious by releasing a functional viral genome following stresses such as those existing in in vitro culture and interspecific crosse s context. The structure of these eBSV is much longer than a single BSV genome, composed of viral fragments duplicated and more or less extensively rearranged. Wild M. balbisiana diploid genotypes (BB) such as Pisang Klutuk Wulung (PKW) harbour such infectious eBSV belonging to three widespread species of BSV (Goldfinger-BSGFV, Imove – BSIM V and Obino l'Ewai - BSOLV) but are nevertheless resistant to any multiplication of BSV without any visible virus particles. Using deep sequencing of total siRNAs of PKW we underlined the presence of virus-derived small RNA (vsRNA) fro...
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Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot on Brassicae and cau... more Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot on Brassicae and causes disease on cabbage and Arabidopsis for instance. The avrAC gene encodes an Xcc-specific type III effector which is responsible for avirulence on Arabidopsis ecotype Col-0 exclusively when Xcc is inoculated in the leaf vasculature. PCR and dot-blot hybridization performed on a large collection of plant pathogenic bacteria revealed that avrAC is specific to Xcc. The analysis of more than 50 Xcc strains reveals that avrAC displays a very low allelic diversity and belongs to the Xcc variable effectome. Moreover, the presence of avrAC is tightly correlated with an increase in Xcc aggressiveness on susceptible Arabidopsis and nonhost pepper plants. We show that the "Leucine-Rich Repeat" (LRR) and "Filamentation induced by cAMP" (Fic) domains are both required for avirulence on resistant Arabidopsis and aggressiveness on pepper. Interestingly, the Fic domains of the VopS...
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The banana streak disease is due to a complex of distinct Banana streak viruses (BSVs) species sh... more The banana streak disease is due to a complex of distinct Banana streak viruses (BSVs) species showing a wide genetic diversity. Banana streak viruses (BSVs) are double stranded DNA pararetroviruses belonging to the family Caulimoviridae, genus Badnavirus. Outbreaks of BSVs causing banana streak disease have been recorded worldwide where Musa spp. is grown during the last 20 years with no convincing evidence of epidemics. Epidemics were previously reported in Uganda where BSV is currently endemic. In addition, the banana genome (Musa sp.) is invaded by numerous badnavirus sequences. The majority of these viral integrants is mostly defective as a result of pseudogenisation driven by the host genome evolution. They are just called BEV (banana endogenous virus sequences) because episomal particles corresponding to the integrated counterparts have not been identified so far. Conversely, only few viral integrants named endogenous BSV (eBSVs) can release a functional BSV genome following ...
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Papers by Matthieu Chabannes