Papers by Maria Starodubtseva
Cytoskeleton
CD109 antigen on the endothelial cell surface plays an important role in vascular pathology. The ... more CD109 antigen on the endothelial cell surface plays an important role in vascular pathology. The aim of the work was to investigate the effect of the immobilization of CD109 antigen with specific antibodies on nanomechanical properties of human umbilical endothelial cells (HUVECs) using atomic force microscopy in quantitative nanomechanical property mapping mode (PeakForce QNM). Anti‐CD109 antibodies induced significant stiffening of the cell surface Me(LQ; UQ): in 1.45(1.07;2.29) times with respect to control cells for fixed cells and in 4.9(3.6;5.9) times with respect to control cells for living cells, and changes in the spatial distribution of cell surface mechanical properties. The changes in the HUVEC's mechanical properties were accompanied by the activation of the TGF‐/Smad2/3 signaling pathway and reorganization of the vimentin and actin cytoskeletal elements. Our findings show that blocking CD109 antigen using anti‐CD109 antibodies leads in HUVECs to the processes simil...
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Health and Ecology Issues
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International Journal of Molecular Sciences
Background: Fungal infections can pose great threat to sight. Immediate treatment is usually requ... more Background: Fungal infections can pose great threat to sight. Immediate treatment is usually required; antifungal agents are widely accepted and are effective in most cases. The present experimental study aims to investigate the probable effects of intravitreal injection of antifungal agents on the structure and mechanical properties of the surface of peripheral blood erythrocytes. Methods: Nine albino New Zealand white rabbits, aged five months old, were chosen for the experiment. Solutions of micafungin, voriconazole, or balanced salt solution (BSS) were injected into the midvitreous. Animals were divided into two experimental groups and one control group. Blood sampling from an intravenous (IV) line was performed after 10 days from the last IV injection. An atomic force microscope (AFM) was used to study the structural and mechanical properties of cell surfaces. Results: The analysis results showed that the parameters of the cytoskeleton’s spatial organization changed insignifica...
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Health and Ecology Issues, 2017
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Health and Ecology Issues, 2015
Atomic force microscopy (AFM) makes it possible to create not only the geometrical image of the s... more Atomic force microscopy (AFM) makes it possible to create not only the geometrical image of the surface of an object but also its physical and mechanical image composed from a number of AFM parameters. Changes of the physical and mechanical AFM image of the erythrocyte surface in patients with infectious respiratory diseases and hepatocirrhosis as well as in the process of erythrocyte ageing have been studied in vitro . The AFM images are based on data obtained during its scanning in the contact mode and include fractal dimension and statistical parameters for the cell surface distribution of lateral forces along the cell surface (sliding friction forces between the point of the AFM probe and the cell surface). The obtained data have shown the effectiveness of the application of the physical and mechanical image of erythrocyte surface in the early detection of their function changes and prognosis of possible circulatory disturbances.
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Health and Ecology Issues, 2014
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Health and Ecology Issues, 2018
Objective: to study the shape of erythrocytes and structure of their surface layer including the ... more Objective: to study the shape of erythrocytes and structure of their surface layer including the membrane and cytoskeleton (actin-spectrin network) in child patients with hereditary spherocytosis. Material and methods. The methods of optic and atomic-force microscopy were used in the study. Results. A variety of erythrocyte shapes with such prevalent types as discocytes, spherocytes, and echinocytes were revealed in the blood of the patients. The surface of certain cells contained microvesicules. The spatial heterogeneity of the structure of mechanical property maps of the cell surface layer was detected. Conclusion. The diversity of erythrocyte features in patients with hereditary spherocytosis is present both at the level of the cell shapes and at the level of the structure of mechanical property maps of their surface layer.
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Health and Ecology Issues, 2020
Objective: to detect changes in the parameters of the structure and mechanical properties of the ... more Objective: to detect changes in the parameters of the structure and mechanical properties of the surface layer of lymphocytes in the blood of a rat after a single action (30 min and 2 h) of an extremely low frequency electromagnetic field (ELF EMF) on the rat`s whole blood in vitro.Material and methods. We exposed the whole blood of a Wistar rat (4.5 months) to the effect of ELF EMF (50 Hz, B = 0.79 mT), generated by an experimental setup based on a Helmholtz coil. Lymphocytes were isolated from the blood using the Histopaque-1077 density gradient sedimentation method, then were placed on the surface of glass slides and fixed with glutaraldehyde, and finally were dried. We performed atomic force microscopy (AFM) using the atomic force microscope Bioscope Resolve in the PeakForce QMN mode in air.Results. On the basis of the results of the analysis of the AFM images, we classified the lymphocytes into several morphological types depending on the size of the lamellopodium. We analyzed ...
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Health and Ecology Issues, 2020
Objective: to identify the difference of the numerical values of parameters characterizing the ge... more Objective: to identify the difference of the numerical values of parameters characterizing the geometric and mechanical (adhesive) properties of inorganic nanosized particles and nanosized particles of biological origin by atomic force microscopy using the mode of the mapping of surface features at nanosized resolution.Material and methods. Exosomes isolated from the blood of Af mice by the method of sequential ultracentrifugation were used as bionanoparticles. Silver nanoparticles were used as inorganic nanoparticles. The nanoparticles were scanned in air with the help of the BioScope Resolve (Bruker) atomic force microscope in the PeakForce QNM in Air mode with the recording of the maps of adhesion forces and imaging of the topography of the studied surfaces.Results. The silver nanoparticles and exosomes had similar but statistically different diameters (45.59 ± 1.04 nm and 41.25 ± 0.91 nm, р < 0.001 t-test). Nevertheless, the silver nanoparticles were characterized by higher v...
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Biology, 2020
While extracellular vesicles (EVs) are extensively studied by various practical applications in b... more While extracellular vesicles (EVs) are extensively studied by various practical applications in biomedicine, there is still little information on their biomechanical properties due to their nanoscale size. We identified isolated blood plasma vesicles that carried on biomarkers associated with exosomes and exomeres and applied atomic force microscopy (AFM) to study them at single particle level in air and in liquid. Air measurements of exosomes revealed a mechanically indented internal cavity in which highly adhesive sites were located. In contrast, the highly adhesive sites of exomeres were located at the periphery and the observed diameter of the particles was ~35 nm. In liquid, the reversible deformation of the internal cavity of exosomes was observed and a slightly deformed lipid bi-layer was identified. In contrast, exomeres were not deformed and their observed diameter was ~16 nm. The difference in diameters might be associated with a higher sorption of water film in air. The p...
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IOP Conference Series: Materials Science and Engineering, 2017
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Atomic force microscopy (AFM) is a unique method to study a single cell surface layer as a biocom... more Atomic force microscopy (AFM) is a unique method to study a single cell surface layer as a biocomposite. This chapter focuses on the advantages of two AFM modes, namely force spectroscopy (providing the information about local elastic properties of cells) and lateral force microscopy (LFM, revealing mechanical heterogeneity of cells surface), to make quantitative analysis of single cells mechanical properties. We demonstrate that LFM parameters (averaged lateral forces and roughness of lateral force map) of single cells (erythrocytes and thymocytes) change under the oxidative stress, with temperature changing and fixing cytoskeleton structure with glutaraldehyde and reflect the state (structural and relaxational) of cortical cytoskeleton. Based on force spectroscopy data we illustrate the difference between elastic behavior of the human embryonic fibroblasts cells and the cells of human lung adenocarcinoma cell line (A549), which can be related to the difference in the cytoskeleton ...
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Biofizika
The formation of two hemoglobin forms (methemoglobin and nitrite methemoglobin) in native human e... more The formation of two hemoglobin forms (methemoglobin and nitrite methemoglobin) in native human erythrocytes in the presence of sodium nitrite in suspension was shown. In normal erythrocytes, the interaction of intracellular oxyhemoglobin with nitrite ions results in the formation of methemoglobin, whereas in metabolically exhausted erythrocytes, this leads predominantly to the formation of nitrite methemoglobin. The nitrite methemoglobin reacts with hydrogen peroxide to form reactive intermediates (e.g. peroxynitrous acid) and the products of hemoglobin destruction. During the storage of erythrocyte suspensions containing methemoglobin and modified nitrite methemoglobin, differences in the forms of erythrocytes and the degree of their hemolysis were revealed. It is assumed that the formation of methemoglobin leads to the destruction of erythrocytes.
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Journal of Applied Spectroscopy, 1999
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Advances in Gerontology, 2012
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Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology, 2010
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Micron, 2012
Atomic force microscopy (AFM) in lateral force mode was applied to assess the microscale thermo-m... more Atomic force microscopy (AFM) in lateral force mode was applied to assess the microscale thermo-mechanical (frictional) properties of the air-dried cell surface in the wide temperature range (288-363K/15-90°C). AFM-investigated cell surface layer can be represented as a biocomposite composed of several layers including the glycocalyx, the membrane and the intercellular layer containing membrane (cortical) cytoskeleton. The cells with two different cytoskeleton structures, erythrocytes and thymocytes, were studied. Above a certain temperature (T(g)), the significant change in friction force with temperature was revealed for the both cell types whereas there was no similar change in their topography parameters. The experimentally determined value T(g) for erythrocyte samples was lower than that for thymocyte ones. Treating living cells with the cross-linking agent, glutaraldehyde, led to the weakening of the temperature dependence of air-dried cell surface frictional properties in the studied temperature range. Addition of oxidizing agent, peroxynitrite, to living cell suspensions changed the temperature dependence of air-dried cell surface frictional properties depending on cell type and peroxynitrite concentration. The obtained data indicate that the study of thermo-mechanical properties of air-dried cells with AFM in lateral force mode provides expanded information on the structural characteristics of the living cell surface layer, and sets the stage for the development of AFM-based method (with using a lateral force mode) for the cell pathology diagnostics.
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Micron, 2007
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Bulletin of Experimental Biology and Medicine, 2007
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Bioelectrochemistry, 2008
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Papers by Maria Starodubtseva