Legume-based green manures (LGMs) are crops that are grown with the specific purpose of improving... more Legume-based green manures (LGMs) are crops that are grown with the specific purpose of improving soil quality and consequently the long-term productivity of crops. Although the traditional focus has been on the supply of nitrogen (N) to the system, they have a wide range of potential benefits that include improving soil quality, reducing soil erosion and increasing the biodiversity of farmland. LGMs are a key component of organic farming systems where the use of synthetic N fertilizers is not permitted. However, increases in the cost of inputs, concerns about environmental impacts of intensive use of agrochemicals, and the recently announced measures for the 'greening' of the European Common Agricultural Policy have led to renewed interest in the use of LGMs more widely. In Europe, the legumes in LGMs may be annual or perennial plants, grown on their own or more often as part of crop mixtures with a range of other crop types such as grasses or brassicas. The legumes most commonly grown are the clovers (Trifolium spp.), particularly red and white clover. Other legumes that may be grown to suit particular local goals or constraints include Medicago spp. (lucerne (alfalfa) and black medic), trefoils (Lotus spp.), vetches (Vicia spp.), lupins (Lupinus spp.), other minor forage legumes and grain legumes. To maximize fertility building in organic farming systems, LGMs are grown in place of cash crops for some of the crop rotation. In more intensive systems, LGMs may be grown for short periods between phases of regular crop production. This chapter reviews the use of LGMs in Europe and considers factors that affect N fixation in them and the transfer of fixed N to following crops. It examines how they can be integrated into practical rotational cropping systems and whether the economics of this makes the use of LGMs profitable. However, LGMs will not be agronomically or economically viable in all systems, and in these cases other types of green manures may be more appropriate. As demand for multifunctional agricultural systems grows, and is increasingly required by European agricultural policies, so does the potential for greater use of LGMs.
A field experiment was carried out to investigate the impact of two improved tillage systems (con... more A field experiment was carried out to investigate the impact of two improved tillage systems (conventional tillage combined with the incorporation of solid anaerobic digestate, no-tillage) on the prokaryotic community composition in two tree orchard (olive, citrus) soils with contrasting texture, carbonate content, and pH, located in Southern Italy. Soil samples were taken over a 5-month period to assess immediate (2 days) vs short-term (7 and 18 weeks) responses. Phylogenetic diversity and compositional shifts of both total and metabolically active soil prokaryotic communities were assessed by next-generation sequencing of 16S rRNA gene templates from soil-extracted DNA/RNA. In both digestate-treated soils, copiotrophic α-Proteobacteria and oligotrophic Acidobacteria, Gemmatimonadetes, and Verrucomicrobia showed an immediate (2 days) but short-lived (7 weeks) shift in their relative abundance similar in persistence but not in magnitude; whereas selective soil type-dependent respons...
Improved soil managements that include reduced soil disturbance and organic amendment incorporati... more Improved soil managements that include reduced soil disturbance and organic amendment incorporation represent valuable strategies to counteract soil degradation processes that affect Mediterranean tree cultivations. However, changes induced by these practices can promote soil N loss through denitrification. Our research aimed to investigate the short-term effects of no-tillage and organic amendment with solid anaerobic digestate on the potential denitrification in two Mediterranean orchard soils showing contrasting properties in terms of texture and pH. Denitrifying enzyme activity (DEA) and selected soil variables (available C and N, microbial biomass C, basal respiration) were monitored in olive and orange tree orchard soils over a five-month period. Our results showed that the application of both practices increased soil DEA, with dynamics that varied according to the soil type. Increased bulk density, lowered soil aeration, and a promoting effect on soil microbial community grow...
The crop yield and quality of seven annual forages (four grasses and three legumes) in sole crop ... more The crop yield and quality of seven annual forages (four grasses and three legumes) in sole crop and in mixtures (ratio 50:50) for oat (Avena sativa L.), Italian ryegrass (Lolium multiflorum Lam.), triticale (x Triticosecale Wittmack), barley (Hordeum vulgare L.), pea (Pisum sativum L.), berseem (Trifolium alexandrinum L.) and common vetch (Vicia sativa L.) were evaluated in a two-year field experiment adopting two harvesting times, green fodder and silage. The main bio-agronomic traits, dry matter forage yield (DMY) and quantity of crude protein (CP) were determined in both sole crop and intercrop. The land equivalent ratio (LER) was used for evaluating biological efficiency and competitive ability of the intercrops. Our results showed that the total calculated LER for fodder and protein yields was always greater than one and corresponded to crop yield advantages of 16.0% and 11.5%, respectively. Our data also highlighted the low competitive ability of the ryegrass in intercrop, wh...
The present investigation was aimed to quantify the three principal components of the soil carbon... more The present investigation was aimed to quantify the three principal components of the soil carbon (C) stock, namely inorganic, organic and permanganate oxidizable, in 0–5 cm and 5–30 cm soil layers, of the main Mediterranean agricultural land coverages: olive grove, olive forest, citrus grove, vineyard, arable irrigated, arable rainfed and natural soil covered by Mediterranean scrub and garrigue. We assessed the contribution of soil properties and climatic variables on soil TOC and POxC by Pearson’s correlations, multiple linear regressions, principal component analyses and variance partitioning. NAT and both olive cropping systems showed the highest TOC concentration while the other land covers showed values ranging between 8.0 and 26.6 g kg−1. Soil POxC represented between 0.5% and 2.2% of the total soil C. A large number of interrelated factors govern soil C accumulation in Mediterranean agroecosystems. Rainfall and temperature had a selective effect on soil C distribution betwee...
During the XX Century, the widespread use of modern wheat cultivars drastically reduced the culti... more During the XX Century, the widespread use of modern wheat cultivars drastically reduced the cultivation of ancient landraces, which nowadays are confined to niche cultivation areas. Several durum wheat landraces adapted to the extreme environments of the Mediterranean region, are still being cultivated in Sicily, Italy. Detailed knowledge of the genetic diversity of this germplasm could lay the basis for their efficient management in breeding programs, for a wide-range range of traits. The aim of the present study was to characterize a collection of durum wheat landraces from Sicily, using single nucleotide polymorphisms (SNP) markers, together with agro-morphological, phenological and quality-related traits. Two modern cv. Simeto, Claudio, and the hexaploid landrace, Cuccitta, were used as outgroups. Cluster analysis and Principal Coordinates Analysis (PCoA) allowed us to identify four main clusters across the analyzed germplasm, among which a cluster included only historical and m...
The potential of biological nitrogen fixation (BNF) to provide sufficient N for production has en... more The potential of biological nitrogen fixation (BNF) to provide sufficient N for production has encouraged re-appraisal of cropping systems that deploy legumes. It has been argued that legume-derived N can maintain productivity as an alternative to the application of mineral fertilizer, although few studies have systematically evaluated the effect of optimizing the balance between legumes and non N-fixing crops to optimize production. In addition, the shortage, or even absence in some regions, of measurements of BNF in crops and forages severely limits the ability to design and evaluate new legume-based agroecosystems. To provide an indication of the magnitude of BNF in European agriculture, a soil-surface N-balance approach was applied to historical data from 8 experimental cropping systems that compared legume and non-legume crop types (e.g., grains, forages and intercrops) across pedoclimatic regions of Europe. Mean BNF for different legume types ranged from 32 to 115 kg ha(-1) an...
The environmental performance of secondgeneration biofuels from lignocellulosic biomass, such as ... more The environmental performance of secondgeneration biofuels from lignocellulosic biomass, such as crop residues, is foreseen to be superior to that of first-generation biofuels such as maize bioethanol. Moreover, using a legume as an intercrop can reduce costly N fertilizers inputs and can increase the N content of the straw mixture. Therefore, we studied cereal-legume intercrops as a source of food grain and straw bioethanol. We grew field pea and four cultivars of durum wheat, triticale, oat, and barley. Crops were grown during 2 years as a sole crop, in additive intercrop with 100 % of the sole crop density for the cereal and 50 % of sole crop density for the legume, and in replacement intercrop with 50 % of the sole crop density for each crop. Tall cereal cultivars were preferred over shorter cereals for an extra production of straw. Results show that growth resources were used up to 23-26 % more efficiently in intercrop than in sole crop for grain and straw production. Tall cereal cultivars suppressed legume yields in intercrops, thus reducing potential intercrop advantages. Pea in intercrop increased straw mixture N by 54 % the first year and by 91 % the second year, versus cereal alone.
INTRODUCTION Intercropping growing two or more crops simulta-neously in the same field is a com... more INTRODUCTION Intercropping growing two or more crops simulta-neously in the same field is a common agricultural practice in the tropics and in the developing coun-tries of the world (Vandermeer, 1989) where yield stability and limited economic risks are the most ...
Complementarity in acquisition of nitrogen (N) from soil and N 2-fixation within pea and barley i... more Complementarity in acquisition of nitrogen (N) from soil and N 2-fixation within pea and barley intercrops was studied in organic field experiments across Western Europe (Denmark, United Kingdom, France, Germany and Italy). Spring pea and barley were sown either as sole crops, at the recommended plant density (P100 and B100, respectively) or in replacement (P50B50) or additive (P100B50) intercropping designs, in each of three cropping seasons (2003-2005). Irrespective of site and intercrop design, Land Equivalent Ratios (LER) between 1.4 at flowering and 1.3 at maturity showed that total N recovery was greater in the pea-barley intercrops than in the sole crops suggesting a high degree of complementarity over a wide range of growing conditions. Complementarity was partly attributed to greater soil mineral N acquisition by barley, forcing pea to rely more on N 2-fixation. At all sites the proportion of total aboveground pea N that was derived from N 2-fixation was greater when intercropped with barley than when grown as a sole crop. No consistent differences were found between the two intercropping designs. Simultaneously, the accumulation of phosphorous (P), potassium (K) and sulphur (S) in Danish and German experiments was 20% higher in the intercrop (P50B50) than in the respective sole crops, possibly influencing general crop yields and thereby competitive ability for other resources. Comparing all sites and seasons, the benefits of organic pea-barley intercropping for N acquisition were highly resilient. It is concluded that pea-barley intercropping is a relevant cropping strategy to adopt when trying to optimize N 2-fixation inputs to the cropping system.
Remote sensing (RS) platforms such as unmanned aerial vehicles (UAVs) represent an essential sour... more Remote sensing (RS) platforms such as unmanned aerial vehicles (UAVs) represent an essential source of information in precision agriculture (PA) as they are able to provide images on a daily basis and at a very high resolution. In this framework, this study aims to identify the optimal level of nitrogen (N)-based nutrients for improved productivity in an onion field of “Cipolla Rossa di Tropea” (Tropea red onion). Following an experiment that involved the arrangement of nine plots in the onion field in a randomized complete block design (RCBD), with three replications, three different levels of N fertilization were compared: N150 (150 kg N ha−1), N180 (180 kg N ha−1), and e N210 (210 kg N ha−1). The crop cycle was monitored using multispectral (MS) UAV imagery, producing vigor maps and taking into account the yield of data. The soil-adjusted vegetation index (SAVI) was used to monitor the vigor of the crop. In addition, the coverage’s class onion was spatially identified using geogr...
In this paper volatile organic compounds (VOCs) from durum wheat cultivars and landraces were ana... more In this paper volatile organic compounds (VOCs) from durum wheat cultivars and landraces were analyzed using PTR-TOF-MS. The aim was to characterize the VOC's profile of the wholemeal flour and of the kernel to find out if any VOCs were specific to varieties and sample matrices. The VOC data is accompanied by SDS-PAGE analyses of the storage proteins (gliadins and glutenins). Statistical analyses was carried out both on the signals obtained by MS and on the protein profiles. The difference between the VOC profile of two cultivars or two preparations of the same sample-matrices, in this case kernel vs wholemeal flour-can be very subtle; the high resolution of PTR-TOF-MS-down to levels as low as pptv-made it possible to recognize these differences. The effects of grinding on the VOC profiles were analyzed using SIMPER and Tanglegram statistical methods. Our results show that it is possible describe samples using VOC profiles and protein data. Wheat is the most cultivated crop in temperate regions, and is widely used for human food and livestock feed 1. The global production of wheat (2015) was 733 MT (http://www.fao.org/worldfoodsituation/csdb/en/). The two most popular species are "bread" or spring wheat (Triticum aestivum L.) and "pasta" or durum wheat (Triticum durum Desf.) that occur as natural intergeneric hybrids 2. About 95% of the wheat currently grown worldwide is hexaploid spring wheat; it is sown in almost every agricultural region of the globe 1. The remaining 5% of the world production is the tetraploid durum wheat, though within the Mediterranean basin it is one of the leading food crops where it is the primary cereal grain used in the production of pasta and certain bread 3,4 products. Italy is the world's top producer of pasta and has the highest pro capita consumption rate (http://www.internationalpasta.org). In Italy, given its economic importance, durum wheat has long been a focus of breeding programs, from which numerous cultivars have derived. Many of these cultivars are currently grown both inside and outside the country. Historically in Italy, we can distinguish three main periods in breeding trends in the 20 th and 21 th century. Landraces and genealogical selections ('old' cultivars) characterized the pre-1950 era. Starting around
Lentil is an important winter-sown legume for semi-arid and temperate areas, food consumption of ... more Lentil is an important winter-sown legume for semi-arid and temperate areas, food consumption of seed is considerable in several countries of Mediterranean Basin. In Central and Southern Italy different lentil landraces are cultivated within specific marginal areas ...
Legume-based green manures (LGMs) are crops that are grown with the specific purpose of improving... more Legume-based green manures (LGMs) are crops that are grown with the specific purpose of improving soil quality and consequently the long-term productivity of crops. Although the traditional focus has been on the supply of nitrogen (N) to the system, they have a wide range of potential benefits that include improving soil quality, reducing soil erosion and increasing the biodiversity of farmland. LGMs are a key component of organic farming systems where the use of synthetic N fertilizers is not permitted. However, increases in the cost of inputs, concerns about environmental impacts of intensive use of agrochemicals, and the recently announced measures for the 'greening' of the European Common Agricultural Policy have led to renewed interest in the use of LGMs more widely. In Europe, the legumes in LGMs may be annual or perennial plants, grown on their own or more often as part of crop mixtures with a range of other crop types such as grasses or brassicas. The legumes most commonly grown are the clovers (Trifolium spp.), particularly red and white clover. Other legumes that may be grown to suit particular local goals or constraints include Medicago spp. (lucerne (alfalfa) and black medic), trefoils (Lotus spp.), vetches (Vicia spp.), lupins (Lupinus spp.), other minor forage legumes and grain legumes. To maximize fertility building in organic farming systems, LGMs are grown in place of cash crops for some of the crop rotation. In more intensive systems, LGMs may be grown for short periods between phases of regular crop production. This chapter reviews the use of LGMs in Europe and considers factors that affect N fixation in them and the transfer of fixed N to following crops. It examines how they can be integrated into practical rotational cropping systems and whether the economics of this makes the use of LGMs profitable. However, LGMs will not be agronomically or economically viable in all systems, and in these cases other types of green manures may be more appropriate. As demand for multifunctional agricultural systems grows, and is increasingly required by European agricultural policies, so does the potential for greater use of LGMs.
A field experiment was carried out to investigate the impact of two improved tillage systems (con... more A field experiment was carried out to investigate the impact of two improved tillage systems (conventional tillage combined with the incorporation of solid anaerobic digestate, no-tillage) on the prokaryotic community composition in two tree orchard (olive, citrus) soils with contrasting texture, carbonate content, and pH, located in Southern Italy. Soil samples were taken over a 5-month period to assess immediate (2 days) vs short-term (7 and 18 weeks) responses. Phylogenetic diversity and compositional shifts of both total and metabolically active soil prokaryotic communities were assessed by next-generation sequencing of 16S rRNA gene templates from soil-extracted DNA/RNA. In both digestate-treated soils, copiotrophic α-Proteobacteria and oligotrophic Acidobacteria, Gemmatimonadetes, and Verrucomicrobia showed an immediate (2 days) but short-lived (7 weeks) shift in their relative abundance similar in persistence but not in magnitude; whereas selective soil type-dependent respons...
Improved soil managements that include reduced soil disturbance and organic amendment incorporati... more Improved soil managements that include reduced soil disturbance and organic amendment incorporation represent valuable strategies to counteract soil degradation processes that affect Mediterranean tree cultivations. However, changes induced by these practices can promote soil N loss through denitrification. Our research aimed to investigate the short-term effects of no-tillage and organic amendment with solid anaerobic digestate on the potential denitrification in two Mediterranean orchard soils showing contrasting properties in terms of texture and pH. Denitrifying enzyme activity (DEA) and selected soil variables (available C and N, microbial biomass C, basal respiration) were monitored in olive and orange tree orchard soils over a five-month period. Our results showed that the application of both practices increased soil DEA, with dynamics that varied according to the soil type. Increased bulk density, lowered soil aeration, and a promoting effect on soil microbial community grow...
The crop yield and quality of seven annual forages (four grasses and three legumes) in sole crop ... more The crop yield and quality of seven annual forages (four grasses and three legumes) in sole crop and in mixtures (ratio 50:50) for oat (Avena sativa L.), Italian ryegrass (Lolium multiflorum Lam.), triticale (x Triticosecale Wittmack), barley (Hordeum vulgare L.), pea (Pisum sativum L.), berseem (Trifolium alexandrinum L.) and common vetch (Vicia sativa L.) were evaluated in a two-year field experiment adopting two harvesting times, green fodder and silage. The main bio-agronomic traits, dry matter forage yield (DMY) and quantity of crude protein (CP) were determined in both sole crop and intercrop. The land equivalent ratio (LER) was used for evaluating biological efficiency and competitive ability of the intercrops. Our results showed that the total calculated LER for fodder and protein yields was always greater than one and corresponded to crop yield advantages of 16.0% and 11.5%, respectively. Our data also highlighted the low competitive ability of the ryegrass in intercrop, wh...
The present investigation was aimed to quantify the three principal components of the soil carbon... more The present investigation was aimed to quantify the three principal components of the soil carbon (C) stock, namely inorganic, organic and permanganate oxidizable, in 0–5 cm and 5–30 cm soil layers, of the main Mediterranean agricultural land coverages: olive grove, olive forest, citrus grove, vineyard, arable irrigated, arable rainfed and natural soil covered by Mediterranean scrub and garrigue. We assessed the contribution of soil properties and climatic variables on soil TOC and POxC by Pearson’s correlations, multiple linear regressions, principal component analyses and variance partitioning. NAT and both olive cropping systems showed the highest TOC concentration while the other land covers showed values ranging between 8.0 and 26.6 g kg−1. Soil POxC represented between 0.5% and 2.2% of the total soil C. A large number of interrelated factors govern soil C accumulation in Mediterranean agroecosystems. Rainfall and temperature had a selective effect on soil C distribution betwee...
During the XX Century, the widespread use of modern wheat cultivars drastically reduced the culti... more During the XX Century, the widespread use of modern wheat cultivars drastically reduced the cultivation of ancient landraces, which nowadays are confined to niche cultivation areas. Several durum wheat landraces adapted to the extreme environments of the Mediterranean region, are still being cultivated in Sicily, Italy. Detailed knowledge of the genetic diversity of this germplasm could lay the basis for their efficient management in breeding programs, for a wide-range range of traits. The aim of the present study was to characterize a collection of durum wheat landraces from Sicily, using single nucleotide polymorphisms (SNP) markers, together with agro-morphological, phenological and quality-related traits. Two modern cv. Simeto, Claudio, and the hexaploid landrace, Cuccitta, were used as outgroups. Cluster analysis and Principal Coordinates Analysis (PCoA) allowed us to identify four main clusters across the analyzed germplasm, among which a cluster included only historical and m...
The potential of biological nitrogen fixation (BNF) to provide sufficient N for production has en... more The potential of biological nitrogen fixation (BNF) to provide sufficient N for production has encouraged re-appraisal of cropping systems that deploy legumes. It has been argued that legume-derived N can maintain productivity as an alternative to the application of mineral fertilizer, although few studies have systematically evaluated the effect of optimizing the balance between legumes and non N-fixing crops to optimize production. In addition, the shortage, or even absence in some regions, of measurements of BNF in crops and forages severely limits the ability to design and evaluate new legume-based agroecosystems. To provide an indication of the magnitude of BNF in European agriculture, a soil-surface N-balance approach was applied to historical data from 8 experimental cropping systems that compared legume and non-legume crop types (e.g., grains, forages and intercrops) across pedoclimatic regions of Europe. Mean BNF for different legume types ranged from 32 to 115 kg ha(-1) an...
The environmental performance of secondgeneration biofuels from lignocellulosic biomass, such as ... more The environmental performance of secondgeneration biofuels from lignocellulosic biomass, such as crop residues, is foreseen to be superior to that of first-generation biofuels such as maize bioethanol. Moreover, using a legume as an intercrop can reduce costly N fertilizers inputs and can increase the N content of the straw mixture. Therefore, we studied cereal-legume intercrops as a source of food grain and straw bioethanol. We grew field pea and four cultivars of durum wheat, triticale, oat, and barley. Crops were grown during 2 years as a sole crop, in additive intercrop with 100 % of the sole crop density for the cereal and 50 % of sole crop density for the legume, and in replacement intercrop with 50 % of the sole crop density for each crop. Tall cereal cultivars were preferred over shorter cereals for an extra production of straw. Results show that growth resources were used up to 23-26 % more efficiently in intercrop than in sole crop for grain and straw production. Tall cereal cultivars suppressed legume yields in intercrops, thus reducing potential intercrop advantages. Pea in intercrop increased straw mixture N by 54 % the first year and by 91 % the second year, versus cereal alone.
INTRODUCTION Intercropping growing two or more crops simulta-neously in the same field is a com... more INTRODUCTION Intercropping growing two or more crops simulta-neously in the same field is a common agricultural practice in the tropics and in the developing coun-tries of the world (Vandermeer, 1989) where yield stability and limited economic risks are the most ...
Complementarity in acquisition of nitrogen (N) from soil and N 2-fixation within pea and barley i... more Complementarity in acquisition of nitrogen (N) from soil and N 2-fixation within pea and barley intercrops was studied in organic field experiments across Western Europe (Denmark, United Kingdom, France, Germany and Italy). Spring pea and barley were sown either as sole crops, at the recommended plant density (P100 and B100, respectively) or in replacement (P50B50) or additive (P100B50) intercropping designs, in each of three cropping seasons (2003-2005). Irrespective of site and intercrop design, Land Equivalent Ratios (LER) between 1.4 at flowering and 1.3 at maturity showed that total N recovery was greater in the pea-barley intercrops than in the sole crops suggesting a high degree of complementarity over a wide range of growing conditions. Complementarity was partly attributed to greater soil mineral N acquisition by barley, forcing pea to rely more on N 2-fixation. At all sites the proportion of total aboveground pea N that was derived from N 2-fixation was greater when intercropped with barley than when grown as a sole crop. No consistent differences were found between the two intercropping designs. Simultaneously, the accumulation of phosphorous (P), potassium (K) and sulphur (S) in Danish and German experiments was 20% higher in the intercrop (P50B50) than in the respective sole crops, possibly influencing general crop yields and thereby competitive ability for other resources. Comparing all sites and seasons, the benefits of organic pea-barley intercropping for N acquisition were highly resilient. It is concluded that pea-barley intercropping is a relevant cropping strategy to adopt when trying to optimize N 2-fixation inputs to the cropping system.
Remote sensing (RS) platforms such as unmanned aerial vehicles (UAVs) represent an essential sour... more Remote sensing (RS) platforms such as unmanned aerial vehicles (UAVs) represent an essential source of information in precision agriculture (PA) as they are able to provide images on a daily basis and at a very high resolution. In this framework, this study aims to identify the optimal level of nitrogen (N)-based nutrients for improved productivity in an onion field of “Cipolla Rossa di Tropea” (Tropea red onion). Following an experiment that involved the arrangement of nine plots in the onion field in a randomized complete block design (RCBD), with three replications, three different levels of N fertilization were compared: N150 (150 kg N ha−1), N180 (180 kg N ha−1), and e N210 (210 kg N ha−1). The crop cycle was monitored using multispectral (MS) UAV imagery, producing vigor maps and taking into account the yield of data. The soil-adjusted vegetation index (SAVI) was used to monitor the vigor of the crop. In addition, the coverage’s class onion was spatially identified using geogr...
In this paper volatile organic compounds (VOCs) from durum wheat cultivars and landraces were ana... more In this paper volatile organic compounds (VOCs) from durum wheat cultivars and landraces were analyzed using PTR-TOF-MS. The aim was to characterize the VOC's profile of the wholemeal flour and of the kernel to find out if any VOCs were specific to varieties and sample matrices. The VOC data is accompanied by SDS-PAGE analyses of the storage proteins (gliadins and glutenins). Statistical analyses was carried out both on the signals obtained by MS and on the protein profiles. The difference between the VOC profile of two cultivars or two preparations of the same sample-matrices, in this case kernel vs wholemeal flour-can be very subtle; the high resolution of PTR-TOF-MS-down to levels as low as pptv-made it possible to recognize these differences. The effects of grinding on the VOC profiles were analyzed using SIMPER and Tanglegram statistical methods. Our results show that it is possible describe samples using VOC profiles and protein data. Wheat is the most cultivated crop in temperate regions, and is widely used for human food and livestock feed 1. The global production of wheat (2015) was 733 MT (http://www.fao.org/worldfoodsituation/csdb/en/). The two most popular species are "bread" or spring wheat (Triticum aestivum L.) and "pasta" or durum wheat (Triticum durum Desf.) that occur as natural intergeneric hybrids 2. About 95% of the wheat currently grown worldwide is hexaploid spring wheat; it is sown in almost every agricultural region of the globe 1. The remaining 5% of the world production is the tetraploid durum wheat, though within the Mediterranean basin it is one of the leading food crops where it is the primary cereal grain used in the production of pasta and certain bread 3,4 products. Italy is the world's top producer of pasta and has the highest pro capita consumption rate (http://www.internationalpasta.org). In Italy, given its economic importance, durum wheat has long been a focus of breeding programs, from which numerous cultivars have derived. Many of these cultivars are currently grown both inside and outside the country. Historically in Italy, we can distinguish three main periods in breeding trends in the 20 th and 21 th century. Landraces and genealogical selections ('old' cultivars) characterized the pre-1950 era. Starting around
Lentil is an important winter-sown legume for semi-arid and temperate areas, food consumption of ... more Lentil is an important winter-sown legume for semi-arid and temperate areas, food consumption of seed is considerable in several countries of Mediterranean Basin. In Central and Southern Italy different lentil landraces are cultivated within specific marginal areas ...
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