Several causes of gingival hyperplasia are known, the most widely accepted being the drug-induced... more Several causes of gingival hyperplasia are known, the most widely accepted being the drug-induced gingival augmentation, a side effect associated mainly with three classes of drugs: anticonvulsants (Phenytoin), immunosuppressants (Cyclosporine A), and various calcium channel blockers (Nifedipine, Verapamil, Diltiazem). We studied the effect of Cyclosporine A (CsA) and Nifedipine on gingival fibroblasts extracted from the rat gum. Gingival fibroblasts were isolated from 6-week-old male rats weighing 150-170 g, from gingival explants, and grown in a specific culture medium consisting of Dulbecco's Modified Eagle's Medium (DMEM) supplemented with antibiotic and 10% fetal bovine serum (FBS). The medium was also supplemented with CsA (1 μg÷mL) and Nifedipine (3 mM). We also used a control group that received no treatment. The cells were photographed at 7, 14 and 30 days, with a Nikon Eclipse TE300 phase contrast microscope. For cell viability evidence, we used immunofluorescence (flow cytometry) with a FACS (fluorescence-activated cell sorting) Calibur device and its software. We noticed that the proliferation of these cells increased with the period of drug administration, and the subsequent morphological changes that occurred were related to the presence of drug accumulations in the cell cytoplasm. Modern molecular techniques are needed to shed some light upon the pathogenesis of drug induced gingival overgrowth and, thereby, provide novel information for preventative and effective future therapeutic strategies.
15th International & 14th European Congress of Endocrinology, May 1, 2012
ABSTRACT Introduction: Recent reports indicate different side effects of the new medication for p... more ABSTRACT Introduction: Recent reports indicate different side effects of the new medication for psoriasis:Adalimumab. Objectives: We report a clinical case of subacute thyroiditis induced by Adalimumab in a psoriatic patient. Materials and Methods: Case report: A 54-year-old Caucasian female addressed to our dermatology clinic in 2008 with a 3-year history of moderate to severe psoriasis. The patient had been experiencing non-disabling joint pain in both knees and wrists for several years. Her medical history was remarkable for pulmonary sarcoidosis (at the age of 32), arterial hypertension and angina pectoris. The patient was started on Adalimumab 40 mg twice monthly with good clinical evolution, but she was diagnosed, a few months after starting the therapy, with subacute thyroiditis with severe evolution, with transitory hyperthyroidism (TSH 0.1 uiu/ml).The treatment with Adalimumab was discontinued, the symptoms cleared in 3 weeks with nonsteroidal antiinflammatory drugs and a fully recovered thyroid status was obtained in one month. The patient continued the psoriatic medication (Adalimumab) with no influence on thyroid status. Results: We describe a case of subacute thyroiditis in a psoriatic patient treated with Adalimumab, with a very good clinical evolution with nonsteroidal anti-inflammatory medication. Conclusion: Liaison between dermatologists and, in this case, endocrinologists and rheumatologists, will help to determine the prevalence of these reactions and to provide insights into the very complex mechanisms of both diseases. Disclosure of Interest: None declared.
Bulletin of the Academy of Sciences of Moldova. Medical Sciences
The aim of this study was to study the impact of Tumor Necrosis Factor-α (TNF-α) levels on dental... more The aim of this study was to study the impact of Tumor Necrosis Factor-α (TNF-α) levels on dental caries demage in children with malignant tumors undergoing radiotherapy in the head and neck region. The case-control study included 48 children aged between 1 to 18 years divided into 2 identical groups by structure. The research group (Gr1) consisted of 24 children with malignant tumors in the head and neck region at a distance of 6 months - 2 years after the completion of radiotherapy (RT). 24 conventionally healthy children were included in the control group (Gr0). Were studied indications of carious experience, and the prediction of dental caries (DC) and the complex assessment of caries risk was performed with the application of Software Cariogram . TNF-α in oral fluid (OF) and blood serum was assessed by the method of immunoenzymatic on solid support. The analysis of the results of the study elucidated the high level of TNF-α in OF (5.53 times) and blood serum (10.19 times) of ch...
The present research proposes an assessment of the localized inflammatory burden but also at the ... more The present research proposes an assessment of the localized inflammatory burden but also at the systemic level by quantitating the pro-inflammatory molecules (IL1-a, IL1-b, IL2, IL12, IFN-g, TNF-a) with endo-periodontal lesions. The study was performed on a group of 146 subjects who, following clinical and radiological examinations, were divided into five groups: healthy endo-periodontal patients, patients with periodontitis, patients with moderate periodontitis, patients with severe periodontitis and patients presenting combined endo-periodontal lesions. IL1-a, IL1-b, IL2, IL12, IFN-g, TNF-a were analysed in crevicular fluid by ELISA and serum by fluorescence flowcytometry. IL1-a and IL1-b showed serum and crevicular fluid values significantly higher than the healthy subjects. Values for IL2, IL12, TNF-a and IFN-g measured in crevicular fluid were higher for groups II, III, IV, and V compared to the group of healthy endo-periodontal subjects. IL2 showed significantly higher serum ...
Granuloma annulare (GA) is a granulomatous skin condition that can present with a diversity of cl... more Granuloma annulare (GA) is a granulomatous skin condition that can present with a diversity of clinical manifestations and locations, with an unknown etiology and diagnosed on clinical-pathological grounds/correlations. Although many trigger factors have been described and several pathogenic mechanisms proposed, the etiology of GA remains unknown. We report a case of work-related, isolated, unilateral GA localized on the right palmar area of a young worker, possibly induced by work-related direct trauma.
Angiotensin-converting enzyme inhibitors (ACEI) are widely used drugs nowadays in treating patien... more Angiotensin-converting enzyme inhibitors (ACEI) are widely used drugs nowadays in treating patients diagnosed with cardiovascular disorders. We present two consecutive cases of acquired angioedema caused by the administration of enalapril and lisinopril in patients with indication for ACE-inhibitors therapy. Rigorous follow-up of side effects of ACEI is required, due to these possible life-threatening adverse reactions.
Revista medico-chirurgicală̆ a Societă̆ţ̜ii de Medici ş̧i Naturaliş̧ti din Iaş̧i
NADPH oxidase (nicotinamide adenine dinucleotide phosphate-oxidase), with its generically termed ... more NADPH oxidase (nicotinamide adenine dinucleotide phosphate-oxidase), with its generically termed NOX isoforms, is the major source of ROS (reactive oxigen species) in biological systems. ROS are small oxygen-derived molecules with an important role in various biological processes (physiological or pathological). If under physiological conditions some processes are beneficial and necessary for life, under pathophysiological conditions they are noxious, harmful. NADPH oxidases are present in phagocytes and in a wide variety of nonphagocytic cells. The enzyme generates superoxide by transferring electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen to produce superoxide anion, a reactive free-radical. Structurally, NADPH oxidase is a multicomponent enzyme which includes two integral membrane proteins, glycoprotein gp9 1 Phox and adaptor protein p22(phox), which together form the heterodimeric flavocytochrome b558 that constitutes the core of the...
Infantile hemangiomas are reported in 10-12% of children less than 1 year of age, with ulceration... more Infantile hemangiomas are reported in 10-12% of children less than 1 year of age, with ulceration in about 5-13% of cases. Little is known about the mechanism of this disease and explanations are still being looked for. We present a 4-month-old female infant with haemangioma on the left buttock; the hemangioma was noticed at 2 weeks of age, progressively enlarging and ulcerated.
Aim: To evaluate the number of lesions of psoriasis and to find risk factors for multiple lesions... more Aim: To evaluate the number of lesions of psoriasis and to find risk factors for multiple lesions. Material and Methods: 1,236 patients (male 54.13%, female 45.87%) with psoriasis were seen over a period of 8 years in an Outpatient Clinic. Patients filled out questionnaires containing age at onset, number of lesions and location at the beginning of the disease, gender, type and localization of psoriasis at the time of clinical examination, psoriasis family history, previous treatment, comorbidities, and social status. Results: The number of psoriasis lesions correlates with: onset age of psoriasis (F=8.902, p=0.0029); age at the moment of clinical examination (F=8.902, p=0.0029); residence in rural area (χ2=8.589, p=0.00338, 95%CI); alcohol intake (χ2=16.47, p=0.00005, 95%CI); smoking (χ2=8.408, p=0.00373, 95%CI); occupation: workers/pupils/students (χ2=14.11, p=0.0069, 95%CI). Conclusions: There is a correlation between number of psoriatic lesions and some factors. Multiple lesions...
Morphea is very difficult to treat because of it being recalcitrant to variable therapeutical att... more Morphea is very difficult to treat because of it being recalcitrant to variable therapeutical attempts. A 64 year-old woman, diagnosed on clinical and histological findings with localized morphea, was treated with narrowband UVB and emollients with favorable outcome. Objectively, post-treatment, the plaque was less indurated, softer to palpation, and less hyperpigmented; skin thickness was measured by using high-frequency ultrasound technique(18 MHz) proving a decreased of dermal thickness from 0.4cm to 0.25 cm within 4 months of therapy. Therefore, this treatment procedure could be an option in managing morphea.
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2013
Biologic therapy such as Etanercept, which is a tumor necrosis factor alpha (TNF-α) inhibitor, ha... more Biologic therapy such as Etanercept, which is a tumor necrosis factor alpha (TNF-α) inhibitor, has been extensively used as election therapy in rheumatoid arthritis. The purpose of this case presentation was to inform about the possibility that lichen planus lesions could potentially become complicated by secondary infections in patients treated with Etanercept. Furthermore, we aimed at analyzing if the complication of the cutaneous lesion was coincidental or it was due to the immunosuppressive systemic therapy, and whether the infected lesion would respond to antibiotic therapy. The patient was a 59-year-old woman with rheumatoid arthritis and that have had lichen planus lesions for approximately 25 years. Only recently, she had been received immunosuppressive therapy (Etanercept and Methotrexate). Further on, the lichen planus flared up with a secondary infection determined by a Methicillin-sensitive Staphylococcus aureus. Uncommon myocardial complications were also characteristic...
Several causes of gingival hyperplasia are known, the most widely accepted being the drug-induced... more Several causes of gingival hyperplasia are known, the most widely accepted being the drug-induced gingival augmentation, a side effect associated mainly with three classes of drugs: anticonvulsants (Phenytoin), immunosuppressants (Cyclosporine A), and various calcium channel blockers (Nifedipine, Verapamil, Diltiazem). We studied the effect of Cyclosporine A (CsA) and Nifedipine on gingival fibroblasts extracted from the rat gum. Gingival fibroblasts were isolated from 6-week-old male rats weighing 150-170 g, from gingival explants, and grown in a specific culture medium consisting of Dulbecco's Modified Eagle's Medium (DMEM) supplemented with antibiotic and 10% fetal bovine serum (FBS). The medium was also supplemented with CsA (1 μg÷mL) and Nifedipine (3 mM). We also used a control group that received no treatment. The cells were photographed at 7, 14 and 30 days, with a Nikon Eclipse TE300 phase contrast microscope. For cell viability evidence, we used immunofluorescence (flow cytometry) with a FACS (fluorescence-activated cell sorting) Calibur device and its software. We noticed that the proliferation of these cells increased with the period of drug administration, and the subsequent morphological changes that occurred were related to the presence of drug accumulations in the cell cytoplasm. Modern molecular techniques are needed to shed some light upon the pathogenesis of drug induced gingival overgrowth and, thereby, provide novel information for preventative and effective future therapeutic strategies.
15th International & 14th European Congress of Endocrinology, May 1, 2012
ABSTRACT Introduction: Recent reports indicate different side effects of the new medication for p... more ABSTRACT Introduction: Recent reports indicate different side effects of the new medication for psoriasis:Adalimumab. Objectives: We report a clinical case of subacute thyroiditis induced by Adalimumab in a psoriatic patient. Materials and Methods: Case report: A 54-year-old Caucasian female addressed to our dermatology clinic in 2008 with a 3-year history of moderate to severe psoriasis. The patient had been experiencing non-disabling joint pain in both knees and wrists for several years. Her medical history was remarkable for pulmonary sarcoidosis (at the age of 32), arterial hypertension and angina pectoris. The patient was started on Adalimumab 40 mg twice monthly with good clinical evolution, but she was diagnosed, a few months after starting the therapy, with subacute thyroiditis with severe evolution, with transitory hyperthyroidism (TSH 0.1 uiu/ml).The treatment with Adalimumab was discontinued, the symptoms cleared in 3 weeks with nonsteroidal antiinflammatory drugs and a fully recovered thyroid status was obtained in one month. The patient continued the psoriatic medication (Adalimumab) with no influence on thyroid status. Results: We describe a case of subacute thyroiditis in a psoriatic patient treated with Adalimumab, with a very good clinical evolution with nonsteroidal anti-inflammatory medication. Conclusion: Liaison between dermatologists and, in this case, endocrinologists and rheumatologists, will help to determine the prevalence of these reactions and to provide insights into the very complex mechanisms of both diseases. Disclosure of Interest: None declared.
Bulletin of the Academy of Sciences of Moldova. Medical Sciences
The aim of this study was to study the impact of Tumor Necrosis Factor-α (TNF-α) levels on dental... more The aim of this study was to study the impact of Tumor Necrosis Factor-α (TNF-α) levels on dental caries demage in children with malignant tumors undergoing radiotherapy in the head and neck region. The case-control study included 48 children aged between 1 to 18 years divided into 2 identical groups by structure. The research group (Gr1) consisted of 24 children with malignant tumors in the head and neck region at a distance of 6 months - 2 years after the completion of radiotherapy (RT). 24 conventionally healthy children were included in the control group (Gr0). Were studied indications of carious experience, and the prediction of dental caries (DC) and the complex assessment of caries risk was performed with the application of Software Cariogram . TNF-α in oral fluid (OF) and blood serum was assessed by the method of immunoenzymatic on solid support. The analysis of the results of the study elucidated the high level of TNF-α in OF (5.53 times) and blood serum (10.19 times) of ch...
The present research proposes an assessment of the localized inflammatory burden but also at the ... more The present research proposes an assessment of the localized inflammatory burden but also at the systemic level by quantitating the pro-inflammatory molecules (IL1-a, IL1-b, IL2, IL12, IFN-g, TNF-a) with endo-periodontal lesions. The study was performed on a group of 146 subjects who, following clinical and radiological examinations, were divided into five groups: healthy endo-periodontal patients, patients with periodontitis, patients with moderate periodontitis, patients with severe periodontitis and patients presenting combined endo-periodontal lesions. IL1-a, IL1-b, IL2, IL12, IFN-g, TNF-a were analysed in crevicular fluid by ELISA and serum by fluorescence flowcytometry. IL1-a and IL1-b showed serum and crevicular fluid values significantly higher than the healthy subjects. Values for IL2, IL12, TNF-a and IFN-g measured in crevicular fluid were higher for groups II, III, IV, and V compared to the group of healthy endo-periodontal subjects. IL2 showed significantly higher serum ...
Granuloma annulare (GA) is a granulomatous skin condition that can present with a diversity of cl... more Granuloma annulare (GA) is a granulomatous skin condition that can present with a diversity of clinical manifestations and locations, with an unknown etiology and diagnosed on clinical-pathological grounds/correlations. Although many trigger factors have been described and several pathogenic mechanisms proposed, the etiology of GA remains unknown. We report a case of work-related, isolated, unilateral GA localized on the right palmar area of a young worker, possibly induced by work-related direct trauma.
Angiotensin-converting enzyme inhibitors (ACEI) are widely used drugs nowadays in treating patien... more Angiotensin-converting enzyme inhibitors (ACEI) are widely used drugs nowadays in treating patients diagnosed with cardiovascular disorders. We present two consecutive cases of acquired angioedema caused by the administration of enalapril and lisinopril in patients with indication for ACE-inhibitors therapy. Rigorous follow-up of side effects of ACEI is required, due to these possible life-threatening adverse reactions.
Revista medico-chirurgicală̆ a Societă̆ţ̜ii de Medici ş̧i Naturaliş̧ti din Iaş̧i
NADPH oxidase (nicotinamide adenine dinucleotide phosphate-oxidase), with its generically termed ... more NADPH oxidase (nicotinamide adenine dinucleotide phosphate-oxidase), with its generically termed NOX isoforms, is the major source of ROS (reactive oxigen species) in biological systems. ROS are small oxygen-derived molecules with an important role in various biological processes (physiological or pathological). If under physiological conditions some processes are beneficial and necessary for life, under pathophysiological conditions they are noxious, harmful. NADPH oxidases are present in phagocytes and in a wide variety of nonphagocytic cells. The enzyme generates superoxide by transferring electrons from NADPH inside the cell across the membrane and coupling them to molecular oxygen to produce superoxide anion, a reactive free-radical. Structurally, NADPH oxidase is a multicomponent enzyme which includes two integral membrane proteins, glycoprotein gp9 1 Phox and adaptor protein p22(phox), which together form the heterodimeric flavocytochrome b558 that constitutes the core of the...
Infantile hemangiomas are reported in 10-12% of children less than 1 year of age, with ulceration... more Infantile hemangiomas are reported in 10-12% of children less than 1 year of age, with ulceration in about 5-13% of cases. Little is known about the mechanism of this disease and explanations are still being looked for. We present a 4-month-old female infant with haemangioma on the left buttock; the hemangioma was noticed at 2 weeks of age, progressively enlarging and ulcerated.
Aim: To evaluate the number of lesions of psoriasis and to find risk factors for multiple lesions... more Aim: To evaluate the number of lesions of psoriasis and to find risk factors for multiple lesions. Material and Methods: 1,236 patients (male 54.13%, female 45.87%) with psoriasis were seen over a period of 8 years in an Outpatient Clinic. Patients filled out questionnaires containing age at onset, number of lesions and location at the beginning of the disease, gender, type and localization of psoriasis at the time of clinical examination, psoriasis family history, previous treatment, comorbidities, and social status. Results: The number of psoriasis lesions correlates with: onset age of psoriasis (F=8.902, p=0.0029); age at the moment of clinical examination (F=8.902, p=0.0029); residence in rural area (χ2=8.589, p=0.00338, 95%CI); alcohol intake (χ2=16.47, p=0.00005, 95%CI); smoking (χ2=8.408, p=0.00373, 95%CI); occupation: workers/pupils/students (χ2=14.11, p=0.0069, 95%CI). Conclusions: There is a correlation between number of psoriatic lesions and some factors. Multiple lesions...
Morphea is very difficult to treat because of it being recalcitrant to variable therapeutical att... more Morphea is very difficult to treat because of it being recalcitrant to variable therapeutical attempts. A 64 year-old woman, diagnosed on clinical and histological findings with localized morphea, was treated with narrowband UVB and emollients with favorable outcome. Objectively, post-treatment, the plaque was less indurated, softer to palpation, and less hyperpigmented; skin thickness was measured by using high-frequency ultrasound technique(18 MHz) proving a decreased of dermal thickness from 0.4cm to 0.25 cm within 4 months of therapy. Therefore, this treatment procedure could be an option in managing morphea.
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2013
Biologic therapy such as Etanercept, which is a tumor necrosis factor alpha (TNF-α) inhibitor, ha... more Biologic therapy such as Etanercept, which is a tumor necrosis factor alpha (TNF-α) inhibitor, has been extensively used as election therapy in rheumatoid arthritis. The purpose of this case presentation was to inform about the possibility that lichen planus lesions could potentially become complicated by secondary infections in patients treated with Etanercept. Furthermore, we aimed at analyzing if the complication of the cutaneous lesion was coincidental or it was due to the immunosuppressive systemic therapy, and whether the infected lesion would respond to antibiotic therapy. The patient was a 59-year-old woman with rheumatoid arthritis and that have had lichen planus lesions for approximately 25 years. Only recently, she had been received immunosuppressive therapy (Etanercept and Methotrexate). Further on, the lichen planus flared up with a secondary infection determined by a Methicillin-sensitive Staphylococcus aureus. Uncommon myocardial complications were also characteristic...
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