The benefit achieved by the use of cochlear implants depends among other factors on the number of... more The benefit achieved by the use of cochlear implants depends among other factors on the number of surviving spiral ganglion cells (SGCs). Neurotrophic factors, especially brain-derived neurotrophic factor (BDNF), have a protective effect on spiral ganglions. Coating of the cochlear implant electrode with BDNF-producing cells may provide long-term delivery of the factor. Therefore, the hypothesis that BDNF-producing fibroblasts can enhance cell survival of cultured SGCs was tested. Lentiviral infection of fibroblasts resulted in BDNF production. Conditioned medium obtained from infected fibroblasts was used for the cultivation of SGCs. As a result, improved survival and neurite outgrowth was observed on SGCs. Our results demonstrate that lentivirally infected fibroblasts produce BDNF that has neurotrophic effects on spiral ganglions.
Background: The phenotype of deafness and its mechanisms are morphologically and electrophysiolog... more Background: The phenotype of deafness and its mechanisms are morphologically and electrophysiologically well characterised. However, the molecular mechanisms and the consequences of deafness are poorly understood. Methods: In this study we investigated changes in gene expression profiles in subfractions of the cochlea and the colliculus inferior, a non-cochlear tissue, of normal and deafened (10% Neomycin) rats using the gene-array-technology. RNA was prepared from modiolus (Mo) und sensorineural epithel/lateral wall (SnE/ Lw) und Colliculus inferior (IC), reverse transcribed with gene specific primers, labeled with 3 2 P-dATP and hybridised with its complementary sequences of 1200 rat ESTs. Results: Similar gene expression profiles were detected in Mo- and SnE/Lw in normal as well in deafened rats differing significantly from those found in IC. In deafened animals differences in mRNA levels were determined in IC for 8 genes, in Mo fur 17 genes and in SnE/Lw for 25 genes in comparison to those of normal rats. By using gene-arrays many genes described in the literature previously could be detected. Otherwise most of the genes found in the cochlea are unknown. Conclusions: The gene-array-technology is a valuable tool in otological research for gene expression analysis and, therefore, for comprehensive understanding of molecular processes in the inner ear. Furthermore gene screening for candidate genes could be a big step ahead in developing therapies of diseases of the inner ear.
Abstract- und Posterband - 93. Jahresversammlung der Deutschen Gesellschaft für HNO-Heilkunde, Kopf- und Hals-Chirurgie e.V., Bonn Interface - Fokus Mensch im Zeitalter der technisierten Medizin
Abstract- und Posterband - 93. Jahresversammlung der Deutschen Gesellschaft für HNO-Heilkunde, Kopf- und Hals-Chirurgie e.V., Bonn Interface - Fokus Mensch im Zeitalter der technisierten Medizin
Abstract- und Posterband – 91. Jahresversammlung der Deutschen Gesellschaft für HNO-Heilkunde, Kopf- und Hals-Chirurgie e.V., Bonn – Welche Qualität macht den Unterschied, 2020
Understanding the ecophysiological aspects of plant adaption require better knowledge of the regu... more Understanding the ecophysiological aspects of plant adaption require better knowledge of the regulation of gene expression by environmental factors. Especially environmental adaption of perennial plants, that have adopted a more long-term ecological strategy than annual plants, remains to be elucidated. We are interested in the global changes in genes expression in Populus leaves and have, as a part of the Populus EST program, produced 4842 ESTs from young non-stressed leaves and around 5500 ESTs from senescing leaves. The gene expression pattern in young and senescing leaves was strikingly different: 38% of the clones from young leaves encoded for photosynthetic proteins which are very poorly represented among the senescence ESTs. In contrast, for example metallothionein, proteases, early light-inducible proteins (ELIP), mitochondrial formated dehydrogenase and many senescence-associated genes were abundant among the senescence ESTs. We have also indirectly estimated amount of chlo...
The benefit achieved by the use of cochlear implants depends among other factors on the number of... more The benefit achieved by the use of cochlear implants depends among other factors on the number of surviving spiral ganglion cells (SGCs). Neurotrophic factors, especially brain-derived neurotrophic factor (BDNF), have a protective effect on spiral ganglions. Coating of the cochlear implant electrode with BDNF-producing cells may provide long-term delivery of the factor. Therefore, the hypothesis that BDNF-producing fibroblasts can enhance cell survival of cultured SGCs was tested. Lentiviral infection of fibroblasts resulted in BDNF production. Conditioned medium obtained from infected fibroblasts was used for the cultivation of SGCs. As a result, improved survival and neurite outgrowth was observed on SGCs. Our results demonstrate that lentivirally infected fibroblasts produce BDNF that has neurotrophic effects on spiral ganglions.
Background: The phenotype of deafness and its mechanisms are morphologically and electrophysiolog... more Background: The phenotype of deafness and its mechanisms are morphologically and electrophysiologically well characterised. However, the molecular mechanisms and the consequences of deafness are poorly understood. Methods: In this study we investigated changes in gene expression profiles in subfractions of the cochlea and the colliculus inferior, a non-cochlear tissue, of normal and deafened (10% Neomycin) rats using the gene-array-technology. RNA was prepared from modiolus (Mo) und sensorineural epithel/lateral wall (SnE/ Lw) und Colliculus inferior (IC), reverse transcribed with gene specific primers, labeled with 3 2 P-dATP and hybridised with its complementary sequences of 1200 rat ESTs. Results: Similar gene expression profiles were detected in Mo- and SnE/Lw in normal as well in deafened rats differing significantly from those found in IC. In deafened animals differences in mRNA levels were determined in IC for 8 genes, in Mo fur 17 genes and in SnE/Lw for 25 genes in comparison to those of normal rats. By using gene-arrays many genes described in the literature previously could be detected. Otherwise most of the genes found in the cochlea are unknown. Conclusions: The gene-array-technology is a valuable tool in otological research for gene expression analysis and, therefore, for comprehensive understanding of molecular processes in the inner ear. Furthermore gene screening for candidate genes could be a big step ahead in developing therapies of diseases of the inner ear.
Abstract- und Posterband - 93. Jahresversammlung der Deutschen Gesellschaft für HNO-Heilkunde, Kopf- und Hals-Chirurgie e.V., Bonn Interface - Fokus Mensch im Zeitalter der technisierten Medizin
Abstract- und Posterband - 93. Jahresversammlung der Deutschen Gesellschaft für HNO-Heilkunde, Kopf- und Hals-Chirurgie e.V., Bonn Interface - Fokus Mensch im Zeitalter der technisierten Medizin
Abstract- und Posterband – 91. Jahresversammlung der Deutschen Gesellschaft für HNO-Heilkunde, Kopf- und Hals-Chirurgie e.V., Bonn – Welche Qualität macht den Unterschied, 2020
Understanding the ecophysiological aspects of plant adaption require better knowledge of the regu... more Understanding the ecophysiological aspects of plant adaption require better knowledge of the regulation of gene expression by environmental factors. Especially environmental adaption of perennial plants, that have adopted a more long-term ecological strategy than annual plants, remains to be elucidated. We are interested in the global changes in genes expression in Populus leaves and have, as a part of the Populus EST program, produced 4842 ESTs from young non-stressed leaves and around 5500 ESTs from senescing leaves. The gene expression pattern in young and senescing leaves was strikingly different: 38% of the clones from young leaves encoded for photosynthetic proteins which are very poorly represented among the senescence ESTs. In contrast, for example metallothionein, proteases, early light-inducible proteins (ELIP), mitochondrial formated dehydrogenase and many senescence-associated genes were abundant among the senescence ESTs. We have also indirectly estimated amount of chlo...
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