... STEPHEN F. CHANDLER,2 KEE YOEUP PAEK,3 ENG-CHONG PUA,4 ... If embryo-genic cultures are estab... more ... STEPHEN F. CHANDLER,2 KEE YOEUP PAEK,3 ENG-CHONG PUA,4 ... If embryo-genic cultures are established, they can be used for selection, but these are available in only a few species (BEN-HAYYIM and KOCHBA 1982; RANGAN and VASIL 1983; LEBRUN et al. ...
In Vitro Cellular & Developmental Biology - Plant, 2013
ABSTRACT To improve root growth and production of bioactive compounds such as anthraquinones (AQ)... more ABSTRACT To improve root growth and production of bioactive compounds such as anthraquinones (AQ), phenolics, and flavonoids by adventitious root cultures of Morinda citrifolia, the effects of aeration rate, inoculum density, and Murashige and Skoog (MS) medium salt strengths were investigated using a balloon-type bubble bioreactor. The possible mechanisms underlying changes in activities of enzymic (superoxide dismutase, catalase, guaiacol peroxidase, ascorbate peroxidase) and nonenzymic (vitamin E) antioxidants, phenylalanine ammonia lyase, and stress levels (accumulation of hydrogen peroxide and proline, peroxidation of lipids) were also studied. Low aeration rate (0.05 vvm [air volume/culture volume/min]) accelerated accumulation of root fresh weight and dry weight (DW). High aeration rates (0.1 to 0.3 vvm) stimulated accumulation of AQ, phenolics, and flavonoids and reduced root growth. Low inoculum densities (5 and 10 g l–1) increased accumulation of those metabolites but inhibited root growth. Culture of adventitious roots with high concentrations of MS salts (1× and 1.5× MS) resulted in induction of oxidative stress that strongly inhibited root growth. Overall, an aeration rate of 0.05 vvm, 15 g l–1 inoculum density, and half-strength (0.5×) MS medium were optimal for enhancing accumulation of root dry biomass (4.38 g l–1), AQ (103.08 mg g–1 DW), phenolics (54.81 mg g–1 DW), and flavonoids (49.27 mg g–1 DW).
Methods in molecular biology (Clifton, N.J.), 2016
Panax ginseng C.A. Meyer (Korean ginseng) is a popular herbal medicine. It has been used in Chine... more Panax ginseng C.A. Meyer (Korean ginseng) is a popular herbal medicine. It has been used in Chinese and Oriental medicines since thousands of years. Ginseng products are generally used as a tonic and an adaptogen to resist the adverse influence of a wide range of physical, chemical and biological factors, and to restore homeostasis. Ginsenosides or ginseng saponins are the principal active ingredients of ginseng. Since ginseng cultivation process is very slow and needs specific environment for field cultivation, cell and tissue cultures are sought as alternatives for the production of ginseng biomass and bioactive compounds. In this chapter, we focus on methods of induction of adventitious roots from ginseng roots, establishment of adventitious root suspension cultures using bioreactors, procedures for processing of adventitious roots, and analysis of ginsenosides by high pressure liquid chromatography.
The effects of light generated by monochromic blue, red or mixed radiation from a fluorescent lam... more The effects of light generated by monochromic blue, red or mixed radiation from a fluorescent lamp (FL) with light emitting diodes (LEDs) (blue, red, or far-red) on growth and morphogenesis of marigold and salvia seedlings were investigated and the responses compared with those of plantlets grown under a broad spectrum conventional fluorescent lamp (a 16 h photoperiod per day). Dry
In vitro regenerated shoots of Spathiphyllum from bioreactor were hydroponically cultured for 30 ... more In vitro regenerated shoots of Spathiphyllum from bioreactor were hydroponically cultured for 30 days. The response of plant growth and photosynthesis to different substrates, photosynthetic photon flux (PPF), nutrient scheduling and electrical conductivity (EC) of hydroponic solution were studied. The best plant growth response was observed in perlite based substrates with moderate PFF (70–100μmol m− 2 s− 1). Highest fresh weight, dry weight, shoot length, root length, root number and photosynthetic characteristics ( ...
... However, this medium has been reported as a floral inhibitor (Dickens and van Staden 1988). .... more ... However, this medium has been reported as a floral inhibitor (Dickens and van Staden 1988). ... In: Tan Nhut D, Van Le B, Tran Thanh Van K, Thorpe T (eds) Thin cell layer culture system: regeneration and transformation application. ...
... STEPHEN F. CHANDLER,2 KEE YOEUP PAEK,3 ENG-CHONG PUA,4 ... If embryo-genic cultures are estab... more ... STEPHEN F. CHANDLER,2 KEE YOEUP PAEK,3 ENG-CHONG PUA,4 ... If embryo-genic cultures are established, they can be used for selection, but these are available in only a few species (BEN-HAYYIM and KOCHBA 1982; RANGAN and VASIL 1983; LEBRUN et al. ...
In Vitro Cellular & Developmental Biology - Plant, 2013
ABSTRACT To improve root growth and production of bioactive compounds such as anthraquinones (AQ)... more ABSTRACT To improve root growth and production of bioactive compounds such as anthraquinones (AQ), phenolics, and flavonoids by adventitious root cultures of Morinda citrifolia, the effects of aeration rate, inoculum density, and Murashige and Skoog (MS) medium salt strengths were investigated using a balloon-type bubble bioreactor. The possible mechanisms underlying changes in activities of enzymic (superoxide dismutase, catalase, guaiacol peroxidase, ascorbate peroxidase) and nonenzymic (vitamin E) antioxidants, phenylalanine ammonia lyase, and stress levels (accumulation of hydrogen peroxide and proline, peroxidation of lipids) were also studied. Low aeration rate (0.05 vvm [air volume/culture volume/min]) accelerated accumulation of root fresh weight and dry weight (DW). High aeration rates (0.1 to 0.3 vvm) stimulated accumulation of AQ, phenolics, and flavonoids and reduced root growth. Low inoculum densities (5 and 10 g l–1) increased accumulation of those metabolites but inhibited root growth. Culture of adventitious roots with high concentrations of MS salts (1× and 1.5× MS) resulted in induction of oxidative stress that strongly inhibited root growth. Overall, an aeration rate of 0.05 vvm, 15 g l–1 inoculum density, and half-strength (0.5×) MS medium were optimal for enhancing accumulation of root dry biomass (4.38 g l–1), AQ (103.08 mg g–1 DW), phenolics (54.81 mg g–1 DW), and flavonoids (49.27 mg g–1 DW).
Methods in molecular biology (Clifton, N.J.), 2016
Panax ginseng C.A. Meyer (Korean ginseng) is a popular herbal medicine. It has been used in Chine... more Panax ginseng C.A. Meyer (Korean ginseng) is a popular herbal medicine. It has been used in Chinese and Oriental medicines since thousands of years. Ginseng products are generally used as a tonic and an adaptogen to resist the adverse influence of a wide range of physical, chemical and biological factors, and to restore homeostasis. Ginsenosides or ginseng saponins are the principal active ingredients of ginseng. Since ginseng cultivation process is very slow and needs specific environment for field cultivation, cell and tissue cultures are sought as alternatives for the production of ginseng biomass and bioactive compounds. In this chapter, we focus on methods of induction of adventitious roots from ginseng roots, establishment of adventitious root suspension cultures using bioreactors, procedures for processing of adventitious roots, and analysis of ginsenosides by high pressure liquid chromatography.
The effects of light generated by monochromic blue, red or mixed radiation from a fluorescent lam... more The effects of light generated by monochromic blue, red or mixed radiation from a fluorescent lamp (FL) with light emitting diodes (LEDs) (blue, red, or far-red) on growth and morphogenesis of marigold and salvia seedlings were investigated and the responses compared with those of plantlets grown under a broad spectrum conventional fluorescent lamp (a 16 h photoperiod per day). Dry
In vitro regenerated shoots of Spathiphyllum from bioreactor were hydroponically cultured for 30 ... more In vitro regenerated shoots of Spathiphyllum from bioreactor were hydroponically cultured for 30 days. The response of plant growth and photosynthesis to different substrates, photosynthetic photon flux (PPF), nutrient scheduling and electrical conductivity (EC) of hydroponic solution were studied. The best plant growth response was observed in perlite based substrates with moderate PFF (70–100μmol m− 2 s− 1). Highest fresh weight, dry weight, shoot length, root length, root number and photosynthetic characteristics ( ...
... However, this medium has been reported as a floral inhibitor (Dickens and van Staden 1988). .... more ... However, this medium has been reported as a floral inhibitor (Dickens and van Staden 1988). ... In: Tan Nhut D, Van Le B, Tran Thanh Van K, Thorpe T (eds) Thin cell layer culture system: regeneration and transformation application. ...
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