The administration of estradiol benzoate, 50 μg, and progesterone, 25 mg, daily for three days ha... more The administration of estradiol benzoate, 50 μg, and progesterone, 25 mg, daily for three days has been found to “sensitize” rats to the growth hormone (GH) releasing activity of hypothalamic extracts. At ten minutes following the injection of 90% methanol extracts of 10 porcine hypothalamic fragments, a significant rise in radioimmunoassayable GH was detected (mean increase in pentobarbital anesthetized rats was 28.5 ng/ml ± 12.3 (SE) VS control of 1.6 ng/ml ± 7.9, p < 0.05). The equivalent of 25 hypothalamic fragments gave a significantly larger response. In a series of animals anesthetized with either pentobarbital or ether, 25 or 10-20 hypothalamic equivalents caused significant elevations of RIA-GH and had no depleting effect on pituitary GH concentration. Without steroid pretreatment, significant effects on plasma GH were not observed even with twenty-five hypothalamic equivalents. In steroid pretreated animals, large doses of vasopressin (5 U) and oxytocin (2 U) also induced GH discharge. TRH at a ...
CON MOTIVO DE LA PRESENTACION DEL LIBRO EN DOS TOMOS DE ANITA HOFFMANN "REFRANERO ZOOLOGICO.... more CON MOTIVO DE LA PRESENTACION DEL LIBRO EN DOS TOMOS DE ANITA HOFFMANN "REFRANERO ZOOLOGICO. APOTEGMAS Y OTRAS EXPRESIONES POPULARES SOBRE LOS ANIMALES", LA COMUNIDAD UNIVERSITARIA HIZO UN RECONOCIMIENTO A LA LABOR ININTERRUMPIDA DE 62 ANOS DE LA PRECURSORA DE LA ACAROLOGIA Y EMBLEMA DE ESTA UNIVERSIDAD. EN EL AUDITORIO CARLOS GRAEF DE LA FACULTAD DE CIENCIAS (FC), RENE DRUCKER COLIN, COORDINADOR DE LA INVESTIGACION CIENTIFICA DE ESTA CASA DE ESTUDIOS, SENALO QUE LA ESPLENDIDA PUBLICACION ES RESULTADO DE UN PASATIEMPO SISTEM TICO Y, SIN DUDA, DE LA PASION DE LA autorA POR EL MUNDO DE LA ZOOLOGIA; REVELA EL ENTUSIASMO DE ANITA HOFFMANN POR LA VIDA Y POR LO VIVO. TAMBIEN PARTICIPARON EN EL RECONOCIMIENTO A LA PROFESORA EMERITA DE LA FC RAMON PERALTA Y FABI, DIRECTOR DE LA DEPENDENCIA; TILA MARIA PEREZ, DIRECTORA DEL INSTITUTO DE BIOLOGIA Y DISCIPULA DE LA RECONOCIDA INVESTIGADORA; JUAN MORALES MALACARA, COORDINADOR DEL DEPARTAMENTO DE BIOLOGIA COMPARADA DE LA FC; ARMANDO SAN...
Background: The uncoupling proteins (UCPs) belong to the mitochondrial inner membrane anion carri... more Background: The uncoupling proteins (UCPs) belong to the mitochondrial inner membrane anion carrier superfamily and play an important role in energy homeostasis. UCP-1 is expressed mostly in brown adipose tissue (BAT) and act in the thermogenesis and regulation of energy expenditure. UCP-2 has a role in the metabolism of fatty acids direct and indirectly path insulin secretion. UCP-3 is specific of skeletal muscle and BAT and may affects the adaptive and translocation of fatty acids. Genetic polymorphisms in these proteins have been associated with obesity, as rs1800592 (-3826 A/G) in UCP-1 gene. The rs659366 (-866GA) UCP-2 has being associated with high expression of its RNAm, decrease of obesity risk, and increase of energy expenditure. The rs1800849 (-55CT) UCP-3 has been associated with low risk of type 2 Diabetes Mellitus, but its association with body mass index is controversial. Objective: To analyze the association of the polymorphisms rs1800592 UCP-1, rs659366 UCP-2, and rs...
Revista de investigación clínica; organo del Hospital de Enfermedades de la Nutrición
A total of 1,030 diabetic patients were studied in order to identify factors associated with vari... more A total of 1,030 diabetic patients were studied in order to identify factors associated with various complications. A higher proportion of women was found (64.1%). Using regression analysis of prevalence versus the logarithm of the duration of diabetes, a half-life of 5.14 years was calculated. In the study of complications, peripheral neuropathy, amputations, renal impairment, albuminuria, myocardial infarction, cataract and amaurosis were strongly associated with duration of diabetes rather than with the age of the patient or the age at diagnosis; in contrast, blood pressure and impotence correlated better with the age of the patient. A discriminant function analysis permitted to identify several factors as predictors of diverse complication mainly: the duration of the disease, and previous use of insulin (negative correlation). Other predictors were glycemia, alcoholism, smoking habit and intake of legumes (beans). Albuminuria was assessed with a radioimmunoassay procedure and fo...
Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association, 2014
Hyperglycemia induces tissue damage and complications by mechanisms that produce advanced glycati... more Hyperglycemia induces tissue damage and complications by mechanisms that produce advanced glycation end-products (AGEs) and inflammation.To investigate the factors associated with the progression of complications in Type 2 diabetes patients.We recruited 157 patients (110 women and 47 men) with diabetes for more than 5 years who were non-smokers and did not have current infections or chronic diseases. Patients were grouped according to neuropathy, nephropathy, and retinopathy status: without (I), slight or moderate (II), and severe complications (III). We measured glucose, lipids and HbA1c, low molecular weight AGEs (LMW AGEs), high sensitivity C-reactive protein (CRP), TNF-α, IL-6, and malondialdehyde (MDA). Patients were re-evaluated 1 year later.Patients were 52.2±6.8 years old with 11.0±4.9 years since diagnosis. After 1 year, circulating AGEs increased (p<0.0001) and eGFR decreased (p<0.0007) in groups II and III. IL-6 and MDA decreased in groups I and II. CRP (p<0.029)...
Frequency an severity of physical and emotional symptoms in young female students from two cities... more Frequency an severity of physical and emotional symptoms in young female students from two cities of México (a medium and a large sized city), were studied. An interview was carried out in 447 female adolescents, non seeking treatment, in a cross sectional design (146 from Guadalajara, and 301 from León). The mean age was 16.7 years (16.5 + SD 1.7 and 17.1 +/- 1.9 respectively). Most frequent symptoms for both groups were abdominal bloating, acne, increased appetite, low back pain, polydipsia, and emotional symptoms related to depression such as fatigue, desire to stay at home, desire to be alone and anxiety. Adolescents from Guadalajara reported increased intensity of diverse symptoms in comparison with adolescents from León: Breast tenderness, polydipsia and symptoms of depression. Difference was maintained after adjustment for covariates. In regards to the phase of the menstrual cycle, significantly different symptoms were: Abdominal bloating, breast tenderness, constipation, pru...
To evaluate in a canine model the induction of tolerance to renal transplantation after splenecto... more To evaluate in a canine model the induction of tolerance to renal transplantation after splenectomy and splenosis. This prospective, experimental, comparative, longitudinal study included 4 experimental groups, each comprising 4 dogs. Group 1 (control group) underwent renal transplantation only; group 2 underwent renal transplantation and splenectomy; group 3 underwent renal transplantation and splenosis; and group 4 underwent renal transplantation, splenectomy, and splenosis. Survival and degree of rejection were compared between the 4 groups. Splenosis improved renal function after transplantation, as indicated by increased serum creatinine concentration (group 3, 6.2 mg/dL vs group 1, 12.9 mg/dL). Comparison of weighted survival curves (corrected for degree of rejection) demonstrated a significant difference between group 2 (66.0 days) and group 4 (66.2 days) vs group 1 (52.7 days) and group 3 (41.2 days) (P = .05, Wilcoxon rank sum test). These results suggest that in this experimental model of renal transplantation, splenosis and splenectomy induce clinical tolerance, as indicated by improved renal function and prolonged recipient survival.
Background/Aims: During the first 10 years, two thirds of the patients with type 2 diabetes melli... more Background/Aims: During the first 10 years, two thirds of the patients with type 2 diabetes mellitus (DM) have microalbuminuria (MA). Functional renal reserve (FRR) and its relationship with proteinuria and metabolic control are unknown at the early phases of disease. We investigated the frequency of MA in recently diagnosed type 2 DM patients, and its association with FRR. Methods: We studied 181 type 2 DM patients with less than 6 months since diagnosis. Renal volume, MA, glomerular filtration rate (GFR) and renal plasma flow (ERPF) were evaluated before and after an acute oral protein load in 28 type 2 DM patients (14 with, and 14 without MA), and in 7 healthy subjects. Results: A total of 10.6% of the patients had MA. MA patients had higher cholesterol and triglyceride levels than those normoalbuminuric. Twenty recently diagnosed type 2 diabetic patients showed high basal GFR. Twelve of them had MA and insulin resistance. After the acute oral protein load, the control subjects a...
LHRH release is dependent on the availability of calcium, and prostaglandin E2 is a potent releas... more LHRH release is dependent on the availability of calcium, and prostaglandin E2 is a potent releaser of LHRH. Therefore, we investigated the role of phospholipase A2 (PLA2) on the release of LHRH from the hypothalamus. Four rat hypothalami were perifused with Krebs-Ringer buffer, and after a 60-min preincubation period, PLA2 was applied during 10 min. The LHRH response was determined by RIA of 10-min fractions collected for the next 60 min. PLA2 induced LHRH release in a dose-related manner at amounts of 2, 10, and 50 U. Omission of Ca++ from the medium using EGTA eliminated the PLA2 effect. Indomethacin treatment increased rather than diminished the PLA2 stimulation. Perifusion with melittin, an activator of PLA2, also increased LHRH release. These results are interpreted as a demonstration that PLA2 has a role in the release of LHRH and that a different route of the cyclooxygenase may be involved besides the well known mediation of prostaglandin E2.
The administration of estradiol benzoate, 50 μg, and progesterone, 25 mg, daily for three days ha... more The administration of estradiol benzoate, 50 μg, and progesterone, 25 mg, daily for three days has been found to “sensitize” rats to the growth hormone (GH) releasing activity of hypothalamic extracts. At ten minutes following the injection of 90% methanol extracts of 10 porcine hypothalamic fragments, a significant rise in radioimmunoassayable GH was detected (mean increase in pentobarbital anesthetized rats was 28.5 ng/ml ± 12.3 (SE) VS control of 1.6 ng/ml ± 7.9, p < 0.05). The equivalent of 25 hypothalamic fragments gave a significantly larger response. In a series of animals anesthetized with either pentobarbital or ether, 25 or 10-20 hypothalamic equivalents caused significant elevations of RIA-GH and had no depleting effect on pituitary GH concentration. Without steroid pretreatment, significant effects on plasma GH were not observed even with twenty-five hypothalamic equivalents. In steroid pretreated animals, large doses of vasopressin (5 U) and oxytocin (2 U) also induced GH discharge. TRH at a ...
CON MOTIVO DE LA PRESENTACION DEL LIBRO EN DOS TOMOS DE ANITA HOFFMANN "REFRANERO ZOOLOGICO.... more CON MOTIVO DE LA PRESENTACION DEL LIBRO EN DOS TOMOS DE ANITA HOFFMANN "REFRANERO ZOOLOGICO. APOTEGMAS Y OTRAS EXPRESIONES POPULARES SOBRE LOS ANIMALES", LA COMUNIDAD UNIVERSITARIA HIZO UN RECONOCIMIENTO A LA LABOR ININTERRUMPIDA DE 62 ANOS DE LA PRECURSORA DE LA ACAROLOGIA Y EMBLEMA DE ESTA UNIVERSIDAD. EN EL AUDITORIO CARLOS GRAEF DE LA FACULTAD DE CIENCIAS (FC), RENE DRUCKER COLIN, COORDINADOR DE LA INVESTIGACION CIENTIFICA DE ESTA CASA DE ESTUDIOS, SENALO QUE LA ESPLENDIDA PUBLICACION ES RESULTADO DE UN PASATIEMPO SISTEM TICO Y, SIN DUDA, DE LA PASION DE LA autorA POR EL MUNDO DE LA ZOOLOGIA; REVELA EL ENTUSIASMO DE ANITA HOFFMANN POR LA VIDA Y POR LO VIVO. TAMBIEN PARTICIPARON EN EL RECONOCIMIENTO A LA PROFESORA EMERITA DE LA FC RAMON PERALTA Y FABI, DIRECTOR DE LA DEPENDENCIA; TILA MARIA PEREZ, DIRECTORA DEL INSTITUTO DE BIOLOGIA Y DISCIPULA DE LA RECONOCIDA INVESTIGADORA; JUAN MORALES MALACARA, COORDINADOR DEL DEPARTAMENTO DE BIOLOGIA COMPARADA DE LA FC; ARMANDO SAN...
Background: The uncoupling proteins (UCPs) belong to the mitochondrial inner membrane anion carri... more Background: The uncoupling proteins (UCPs) belong to the mitochondrial inner membrane anion carrier superfamily and play an important role in energy homeostasis. UCP-1 is expressed mostly in brown adipose tissue (BAT) and act in the thermogenesis and regulation of energy expenditure. UCP-2 has a role in the metabolism of fatty acids direct and indirectly path insulin secretion. UCP-3 is specific of skeletal muscle and BAT and may affects the adaptive and translocation of fatty acids. Genetic polymorphisms in these proteins have been associated with obesity, as rs1800592 (-3826 A/G) in UCP-1 gene. The rs659366 (-866GA) UCP-2 has being associated with high expression of its RNAm, decrease of obesity risk, and increase of energy expenditure. The rs1800849 (-55CT) UCP-3 has been associated with low risk of type 2 Diabetes Mellitus, but its association with body mass index is controversial. Objective: To analyze the association of the polymorphisms rs1800592 UCP-1, rs659366 UCP-2, and rs...
Revista de investigación clínica; organo del Hospital de Enfermedades de la Nutrición
A total of 1,030 diabetic patients were studied in order to identify factors associated with vari... more A total of 1,030 diabetic patients were studied in order to identify factors associated with various complications. A higher proportion of women was found (64.1%). Using regression analysis of prevalence versus the logarithm of the duration of diabetes, a half-life of 5.14 years was calculated. In the study of complications, peripheral neuropathy, amputations, renal impairment, albuminuria, myocardial infarction, cataract and amaurosis were strongly associated with duration of diabetes rather than with the age of the patient or the age at diagnosis; in contrast, blood pressure and impotence correlated better with the age of the patient. A discriminant function analysis permitted to identify several factors as predictors of diverse complication mainly: the duration of the disease, and previous use of insulin (negative correlation). Other predictors were glycemia, alcoholism, smoking habit and intake of legumes (beans). Albuminuria was assessed with a radioimmunoassay procedure and fo...
Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association, 2014
Hyperglycemia induces tissue damage and complications by mechanisms that produce advanced glycati... more Hyperglycemia induces tissue damage and complications by mechanisms that produce advanced glycation end-products (AGEs) and inflammation.To investigate the factors associated with the progression of complications in Type 2 diabetes patients.We recruited 157 patients (110 women and 47 men) with diabetes for more than 5 years who were non-smokers and did not have current infections or chronic diseases. Patients were grouped according to neuropathy, nephropathy, and retinopathy status: without (I), slight or moderate (II), and severe complications (III). We measured glucose, lipids and HbA1c, low molecular weight AGEs (LMW AGEs), high sensitivity C-reactive protein (CRP), TNF-α, IL-6, and malondialdehyde (MDA). Patients were re-evaluated 1 year later.Patients were 52.2±6.8 years old with 11.0±4.9 years since diagnosis. After 1 year, circulating AGEs increased (p<0.0001) and eGFR decreased (p<0.0007) in groups II and III. IL-6 and MDA decreased in groups I and II. CRP (p<0.029)...
Frequency an severity of physical and emotional symptoms in young female students from two cities... more Frequency an severity of physical and emotional symptoms in young female students from two cities of México (a medium and a large sized city), were studied. An interview was carried out in 447 female adolescents, non seeking treatment, in a cross sectional design (146 from Guadalajara, and 301 from León). The mean age was 16.7 years (16.5 + SD 1.7 and 17.1 +/- 1.9 respectively). Most frequent symptoms for both groups were abdominal bloating, acne, increased appetite, low back pain, polydipsia, and emotional symptoms related to depression such as fatigue, desire to stay at home, desire to be alone and anxiety. Adolescents from Guadalajara reported increased intensity of diverse symptoms in comparison with adolescents from León: Breast tenderness, polydipsia and symptoms of depression. Difference was maintained after adjustment for covariates. In regards to the phase of the menstrual cycle, significantly different symptoms were: Abdominal bloating, breast tenderness, constipation, pru...
To evaluate in a canine model the induction of tolerance to renal transplantation after splenecto... more To evaluate in a canine model the induction of tolerance to renal transplantation after splenectomy and splenosis. This prospective, experimental, comparative, longitudinal study included 4 experimental groups, each comprising 4 dogs. Group 1 (control group) underwent renal transplantation only; group 2 underwent renal transplantation and splenectomy; group 3 underwent renal transplantation and splenosis; and group 4 underwent renal transplantation, splenectomy, and splenosis. Survival and degree of rejection were compared between the 4 groups. Splenosis improved renal function after transplantation, as indicated by increased serum creatinine concentration (group 3, 6.2 mg/dL vs group 1, 12.9 mg/dL). Comparison of weighted survival curves (corrected for degree of rejection) demonstrated a significant difference between group 2 (66.0 days) and group 4 (66.2 days) vs group 1 (52.7 days) and group 3 (41.2 days) (P = .05, Wilcoxon rank sum test). These results suggest that in this experimental model of renal transplantation, splenosis and splenectomy induce clinical tolerance, as indicated by improved renal function and prolonged recipient survival.
Background/Aims: During the first 10 years, two thirds of the patients with type 2 diabetes melli... more Background/Aims: During the first 10 years, two thirds of the patients with type 2 diabetes mellitus (DM) have microalbuminuria (MA). Functional renal reserve (FRR) and its relationship with proteinuria and metabolic control are unknown at the early phases of disease. We investigated the frequency of MA in recently diagnosed type 2 DM patients, and its association with FRR. Methods: We studied 181 type 2 DM patients with less than 6 months since diagnosis. Renal volume, MA, glomerular filtration rate (GFR) and renal plasma flow (ERPF) were evaluated before and after an acute oral protein load in 28 type 2 DM patients (14 with, and 14 without MA), and in 7 healthy subjects. Results: A total of 10.6% of the patients had MA. MA patients had higher cholesterol and triglyceride levels than those normoalbuminuric. Twenty recently diagnosed type 2 diabetic patients showed high basal GFR. Twelve of them had MA and insulin resistance. After the acute oral protein load, the control subjects a...
LHRH release is dependent on the availability of calcium, and prostaglandin E2 is a potent releas... more LHRH release is dependent on the availability of calcium, and prostaglandin E2 is a potent releaser of LHRH. Therefore, we investigated the role of phospholipase A2 (PLA2) on the release of LHRH from the hypothalamus. Four rat hypothalami were perifused with Krebs-Ringer buffer, and after a 60-min preincubation period, PLA2 was applied during 10 min. The LHRH response was determined by RIA of 10-min fractions collected for the next 60 min. PLA2 induced LHRH release in a dose-related manner at amounts of 2, 10, and 50 U. Omission of Ca++ from the medium using EGTA eliminated the PLA2 effect. Indomethacin treatment increased rather than diminished the PLA2 stimulation. Perifusion with melittin, an activator of PLA2, also increased LHRH release. These results are interpreted as a demonstration that PLA2 has a role in the release of LHRH and that a different route of the cyclooxygenase may be involved besides the well known mediation of prostaglandin E2.
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