Normal human kidneys were examined for the presence of granular peripolar cells. The prominent fe... more Normal human kidneys were examined for the presence of granular peripolar cells. The prominent feature of the human peripolar cell was the presence of numerous cytoplasmic membrane-bound granules, which had the appearance of secretory granules. Most of the granules had a homogeneous matrix with no crystalline substructure. We found no examples of exocytosis, but there were many complex invaginations of the cell membrane. The ultrastructural features of the human peripolar cell are those of a secretory epithelial cell.
SEG Technical Program Expanded Abstracts 2008, 2008
We describe a new algorithm that uses known firing times to separate data from two or more impuls... more We describe a new algorithm that uses known firing times to separate data from two or more impulsive, simultaneous seismic sources. Synthetic and field data tests show that the algorithm works well, especially when the data are not spatially aliased. Aliasing effects ...
A beta-glucoside encoded by a cloned Zea mays complementary DNA (Zm-p60. 1) cleaved the biologica... more A beta-glucoside encoded by a cloned Zea mays complementary DNA (Zm-p60. 1) cleaved the biologically inactive hormone conjugates cytokinin-O-glucosides and kinetin-N3glucoside, releasing active cytokinin. Tobacco protoplasts that transiently expressed Zm-p60.1 could use the inactive cytokinin glucosides to initiate cell division. The ability of protoplasts to sustain growth in response to cytokinin glucosides persisted indefinitely after the likely disappearance of the expression vector. In the roots of maize seedlings, Zm-p60.1 was localized to the meristematic cells and may function in vivo to supply the developing maize embryo with active cytokinin.
Proceedings of the National Academy of Sciences, 1998
A widely applicable promoter system is described that allows a gene of interest to be activated i... more A widely applicable promoter system is described that allows a gene of interest to be activated in specific plant tissues after a cross between defined transgenic lines. The promoter, pOp, consists of lac operators cloned upstream of a minimal promoter. No expression was detected from this promoter when placed upstream of a β-glucuronidase (GUS) reporter gene in transgenic plants. Transcription from the promoter was activated by crossing reporter plants with activator lines that expressed a chimeric transcription factor, LhG4. This factor comprised transcription-activation domain-II from Gal4 of Saccharomyces cerevisiae fused to a mutant lac -repressor that binds its operator with increased affinity. When LhG4 was expressed from the CaMV 35S promoter, the spatial and quantitative expression characteristics of the 35S promoter were exhibited by the GUS reporter. The LhG4/pOp system may be used to study toxic or deleterious gene products, to coordinate the expression of multiple gene ...
Page 1. Nonlinear Mechanical Response of High Density Polyethylene. Part II: Uniaxial Constitutiv... more Page 1. Nonlinear Mechanical Response of High Density Polyethylene. Part II: Uniaxial Constitutive Modeling CHUNTAO ZHANG and IAN D. MOORE Geotechnical Research Centre Faculty of Engineering Science The University ...
Human glomerular capillary tufts were removed by microdissection and scanning electron microscopy... more Human glomerular capillary tufts were removed by microdissection and scanning electron microscopy was used to examine the surface of the capillary tuft and the interior of its Bowman's capsule in order to identify connections between the tuft and capsule. Glomeruli were examined in histologically normal renal cortex from 12 kidneys removed for tumour and 12 renal allografts removed for end-stage rejection. In normal kidney, the glomerular tuft was connected to Bowman's capsule by single podocytes and their processes. At the vascular pole, these were predominantly associated with parietal podocytes which lined Bowman's capsule. At the tubular pole, occasional podocytic processes derived from the capillary tuft bridged Bowman's space and connected to Bowman's capsule where there were no parietal podocytes. These podocytic connections were also found in all rejected transplants, but in addition adhesions were identified which consisted of thicker connections between the tuft and capsule. At the vascular pole, tuft-to-capsule adhesions were found in all 12 kidneys; these were always associated with parietal podocytes. Tubular pole adhesions were identified in ten of the 12 transplants. They were associated with abnormal squamous cells, but not with parietal podocytes. When the capillary tuft herniated into the proximal tubule, the tuft sometimes formed an adhesion with the origin of the proximal tubule. These observations suggest that podocyte connections between the glomerular tuft and Bowman's capsule may be precursors of glomerular adhesions at the vascular pole. Since tuft-to-capsule adhesions at the vascular pole differ morphologically from those at the tubular pole, this may reflect different pathogenetic mechanisms at the opposite poles of the glomerulus.
Normal human kidneys were examined for the presence of granular peripolar cells. The prominent fe... more Normal human kidneys were examined for the presence of granular peripolar cells. The prominent feature of the human peripolar cell was the presence of numerous cytoplasmic membrane-bound granules, which had the appearance of secretory granules. Most of the granules had a homogeneous matrix with no crystalline substructure. We found no examples of exocytosis, but there were many complex invaginations of the cell membrane. The ultrastructural features of the human peripolar cell are those of a secretory epithelial cell.
SEG Technical Program Expanded Abstracts 2008, 2008
We describe a new algorithm that uses known firing times to separate data from two or more impuls... more We describe a new algorithm that uses known firing times to separate data from two or more impulsive, simultaneous seismic sources. Synthetic and field data tests show that the algorithm works well, especially when the data are not spatially aliased. Aliasing effects ...
A beta-glucoside encoded by a cloned Zea mays complementary DNA (Zm-p60. 1) cleaved the biologica... more A beta-glucoside encoded by a cloned Zea mays complementary DNA (Zm-p60. 1) cleaved the biologically inactive hormone conjugates cytokinin-O-glucosides and kinetin-N3glucoside, releasing active cytokinin. Tobacco protoplasts that transiently expressed Zm-p60.1 could use the inactive cytokinin glucosides to initiate cell division. The ability of protoplasts to sustain growth in response to cytokinin glucosides persisted indefinitely after the likely disappearance of the expression vector. In the roots of maize seedlings, Zm-p60.1 was localized to the meristematic cells and may function in vivo to supply the developing maize embryo with active cytokinin.
Proceedings of the National Academy of Sciences, 1998
A widely applicable promoter system is described that allows a gene of interest to be activated i... more A widely applicable promoter system is described that allows a gene of interest to be activated in specific plant tissues after a cross between defined transgenic lines. The promoter, pOp, consists of lac operators cloned upstream of a minimal promoter. No expression was detected from this promoter when placed upstream of a β-glucuronidase (GUS) reporter gene in transgenic plants. Transcription from the promoter was activated by crossing reporter plants with activator lines that expressed a chimeric transcription factor, LhG4. This factor comprised transcription-activation domain-II from Gal4 of Saccharomyces cerevisiae fused to a mutant lac -repressor that binds its operator with increased affinity. When LhG4 was expressed from the CaMV 35S promoter, the spatial and quantitative expression characteristics of the 35S promoter were exhibited by the GUS reporter. The LhG4/pOp system may be used to study toxic or deleterious gene products, to coordinate the expression of multiple gene ...
Page 1. Nonlinear Mechanical Response of High Density Polyethylene. Part II: Uniaxial Constitutiv... more Page 1. Nonlinear Mechanical Response of High Density Polyethylene. Part II: Uniaxial Constitutive Modeling CHUNTAO ZHANG and IAN D. MOORE Geotechnical Research Centre Faculty of Engineering Science The University ...
Human glomerular capillary tufts were removed by microdissection and scanning electron microscopy... more Human glomerular capillary tufts were removed by microdissection and scanning electron microscopy was used to examine the surface of the capillary tuft and the interior of its Bowman's capsule in order to identify connections between the tuft and capsule. Glomeruli were examined in histologically normal renal cortex from 12 kidneys removed for tumour and 12 renal allografts removed for end-stage rejection. In normal kidney, the glomerular tuft was connected to Bowman's capsule by single podocytes and their processes. At the vascular pole, these were predominantly associated with parietal podocytes which lined Bowman's capsule. At the tubular pole, occasional podocytic processes derived from the capillary tuft bridged Bowman's space and connected to Bowman's capsule where there were no parietal podocytes. These podocytic connections were also found in all rejected transplants, but in addition adhesions were identified which consisted of thicker connections between the tuft and capsule. At the vascular pole, tuft-to-capsule adhesions were found in all 12 kidneys; these were always associated with parietal podocytes. Tubular pole adhesions were identified in ten of the 12 transplants. They were associated with abnormal squamous cells, but not with parietal podocytes. When the capillary tuft herniated into the proximal tubule, the tuft sometimes formed an adhesion with the origin of the proximal tubule. These observations suggest that podocyte connections between the glomerular tuft and Bowman's capsule may be precursors of glomerular adhesions at the vascular pole. Since tuft-to-capsule adhesions at the vascular pole differ morphologically from those at the tubular pole, this may reflect different pathogenetic mechanisms at the opposite poles of the glomerulus.
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