The use of serious games, virtual patients, and other forms of digital learning technologies are ... more The use of serious games, virtual patients, and other forms of digital learning technologies are starting to emerge in dentistry schools around the world. However, because of their novelty, there is still a need for literature discussing the different challenges of computer-supported education in this field. This paper presents the design, development, and pilot validation of a serious game for the development of diagnostic skills in dentistry students. Called RealTeeth, this game puts the student in a job interview context and asks him to diagnose 10 endondontic cases. Each case allows the student to follow 5 different diagnostic routes with different information and resources. At the end of the 10 cases, the student will receive a job offer in accordance with his or her performance on the cases. This game was tested with a cohort of student of the pre-clinic course of endodontics in the Pontificia Universidad Javeriana in Bogotá, Colombia. The results of this pilot validation were...
Summary: Introduction: different factors participate in the pathogenesis ofperiodontal diseases. ... more Summary: Introduction: different factors participate in the pathogenesis ofperiodontal diseases. One factor is the interaction between the fibroblasts and derived productsfrom the microorganisms found in the periodontal environment. Objective: In this work, cultures ofhuman gingival fibroblasts from a healthy donor were used to characterize the in vitro responses tobacterial lipopolysaccharide. Methods: the proliferative response was evaluated using cell count andexpression of CD14 was assessed by flow cytometry. Results: after 24 hours of culture an increase inthe cell number was detected in cultures treated with 1.0 mg/mL LPS, but these differences were notstatistically significant. Human gingival fibroblasts express CD14, but its expression decreases incells cultivated after a short period of time. Nevertheless, lipopolysaccharide helps to recover theexpression of CD14 in fibroblast after 24 hours of culture. Conclusion: preliminary result suggeststhat expression of CD14 on gingi...
Resumo: La capsaicina es un vanilloide natural que controla la inflamación neurogénica disminuyen... more Resumo: La capsaicina es un vanilloide natural que controla la inflamación neurogénica disminuyendo los neuropeptidos en la sinapsis neuronal. Nosotros determinamos el efecto de la capsaicina sobre la proliferación y el ciclo celular de fibroblastos pulpares humanos ...
La capsaicina es un alcaloide natural, que permite el control de la inflamación neurogénica al di... more La capsaicina es un alcaloide natural, que permite el control de la inflamación neurogénica al disminuir los niveles de neuropéptidos como la SP y el CGRP, por lo que podría llegar a ser considerada, una herramienta farmacológica para el manejo terapéutico de la inflamación pulpar. El objetivo de la presente investigación fue determinar el efecto de la capsaicina sobre el ciclo celular de fibroblastos pulpares humanos (FPH) en cultivo, pensando en el potencial de esta célula en los procesos de reparación pulpar. Utilizando citometría de flujo y una marcación con Yoduro de propidio , se obtuvo una distribución porcentual del número de FPH, de acuerdo con la fase del ciclo celular, después de la estimulación con capsaicina 10-4M, 10-8M y 10-16M, a las 24, 48, 72 y 96 horas. Se encontró que el efecto de la capsaicina sobre los FPH en cultivo, depende de la dosis y el tiempo. El mayor porcentaje de células en proceso de división celular entre las 24 y las 96 horas, se logró con capsaici...
Dental caries is an infectious disease which still constitutes a public health concern. It begins... more Dental caries is an infectious disease which still constitutes a public health concern. It begins at an early age and is caused mainly Streptococcus mutans (S. mutans). The aim of this study was to characterize the salivary humor immune response to S. mutans proteins in patients with caries, with history of caries and without caries, in order to determine which S. mutans proteins participate in the immunological response in subjects with different caries experience. Saliva was collected by spontaneous salivation for 5 minutes from 60 subjects aged 18 to 30 years, classified according to their caries experience as: without caries (Group I), with active caries (Group II) and with history of caries (Group III). The antigens derived from S. mutans by sonication were recognized by salivary IgA and IgG by Western Blot. The results showed that all the individuals studied recognized S. mutans proteins with molecular weights in the range of 8 to 191 kDa, with similar recognition profiles for...
The purpose of this study was to quantify the percentage and the mean fluorescence intensity of v... more The purpose of this study was to quantify the percentage and the mean fluorescence intensity of viable alternatively activated monocytes/macrophages (AAMø) CD163+ positive for calcitonin gene-related peptide receptor (CGRPr) within the total AAMø population in human dental pulp. Pulp tissue samples were collected from teeth with a clinical diagnosis of irreversible pulpitis (n = 13), pulps with induced inflammation (n = 13), and normal pulps (n = 13). All samples were labeled to identify positive cells for CGRPr and CD163 using a flow cytometry assay. Results demonstrated that a high percentage of total viable AAMø CD163+ expressed CGRPr on their membranes (72.12% in healthy pulp, 62.20% in irreversible pulpitis, and 58.01% in induced pulpitis). Significant differences were found between mean AAMø CD163+ fluorescence for CGRPr according to pulp condition, being greater in irreversible pulpitis. It can be concluded that AAMø CD163+ are expressed during normal and inflammatory process...
The purpose of this study was to quantify the percentage and the mean fluorescence intensity of v... more The purpose of this study was to quantify the percentage and the mean fluorescence intensity of viable alternatively activated monocytes/macrophages (AAMø) CD163+ positive for calcitonin gene-related peptide receptor (CGRPr) within the total AAMø population in human dental ...
Understanding pulp repair and regeneration requires being familiar with this tissue's behavi... more Understanding pulp repair and regeneration requires being familiar with this tissue's behavior under extreme conditions, such as postmortem state where an abrupt interruption of tissue blood supply occurs. The purpose of this study was to quantify cell viability and the amount of ...
Insulin-like growth factor-1 (IGF-1) plays an important role in cell proliferation and differenti... more Insulin-like growth factor-1 (IGF-1) plays an important role in cell proliferation and differentiation. The purpose of this study was to use a radioreceptor assay to evaluate whether IGF-1 receptors are present in human pulp and to determine whether differences in its ...
The use of serious games, virtual patients, and other forms of digital learning technologies are ... more The use of serious games, virtual patients, and other forms of digital learning technologies are starting to emerge in dentistry schools around the world. However, because of their novelty, there is still a need for literature discussing the different challenges of computer-supported education in this field. This paper presents the design, development, and pilot validation of a serious game for the development of diagnostic skills in dentistry students. Called RealTeeth, this game puts the student in a job interview context and asks him to diagnose 10 endondontic cases. Each case allows the student to follow 5 different diagnostic routes with different information and resources. At the end of the 10 cases, the student will receive a job offer in accordance with his or her performance on the cases. This game was tested with a cohort of student of the pre-clinic course of endodontics in the Pontificia Universidad Javeriana in Bogotá, Colombia. The results of this pilot validation were...
Summary: Introduction: different factors participate in the pathogenesis ofperiodontal diseases. ... more Summary: Introduction: different factors participate in the pathogenesis ofperiodontal diseases. One factor is the interaction between the fibroblasts and derived productsfrom the microorganisms found in the periodontal environment. Objective: In this work, cultures ofhuman gingival fibroblasts from a healthy donor were used to characterize the in vitro responses tobacterial lipopolysaccharide. Methods: the proliferative response was evaluated using cell count andexpression of CD14 was assessed by flow cytometry. Results: after 24 hours of culture an increase inthe cell number was detected in cultures treated with 1.0 mg/mL LPS, but these differences were notstatistically significant. Human gingival fibroblasts express CD14, but its expression decreases incells cultivated after a short period of time. Nevertheless, lipopolysaccharide helps to recover theexpression of CD14 in fibroblast after 24 hours of culture. Conclusion: preliminary result suggeststhat expression of CD14 on gingi...
Resumo: La capsaicina es un vanilloide natural que controla la inflamación neurogénica disminuyen... more Resumo: La capsaicina es un vanilloide natural que controla la inflamación neurogénica disminuyendo los neuropeptidos en la sinapsis neuronal. Nosotros determinamos el efecto de la capsaicina sobre la proliferación y el ciclo celular de fibroblastos pulpares humanos ...
La capsaicina es un alcaloide natural, que permite el control de la inflamación neurogénica al di... more La capsaicina es un alcaloide natural, que permite el control de la inflamación neurogénica al disminuir los niveles de neuropéptidos como la SP y el CGRP, por lo que podría llegar a ser considerada, una herramienta farmacológica para el manejo terapéutico de la inflamación pulpar. El objetivo de la presente investigación fue determinar el efecto de la capsaicina sobre el ciclo celular de fibroblastos pulpares humanos (FPH) en cultivo, pensando en el potencial de esta célula en los procesos de reparación pulpar. Utilizando citometría de flujo y una marcación con Yoduro de propidio , se obtuvo una distribución porcentual del número de FPH, de acuerdo con la fase del ciclo celular, después de la estimulación con capsaicina 10-4M, 10-8M y 10-16M, a las 24, 48, 72 y 96 horas. Se encontró que el efecto de la capsaicina sobre los FPH en cultivo, depende de la dosis y el tiempo. El mayor porcentaje de células en proceso de división celular entre las 24 y las 96 horas, se logró con capsaici...
Dental caries is an infectious disease which still constitutes a public health concern. It begins... more Dental caries is an infectious disease which still constitutes a public health concern. It begins at an early age and is caused mainly Streptococcus mutans (S. mutans). The aim of this study was to characterize the salivary humor immune response to S. mutans proteins in patients with caries, with history of caries and without caries, in order to determine which S. mutans proteins participate in the immunological response in subjects with different caries experience. Saliva was collected by spontaneous salivation for 5 minutes from 60 subjects aged 18 to 30 years, classified according to their caries experience as: without caries (Group I), with active caries (Group II) and with history of caries (Group III). The antigens derived from S. mutans by sonication were recognized by salivary IgA and IgG by Western Blot. The results showed that all the individuals studied recognized S. mutans proteins with molecular weights in the range of 8 to 191 kDa, with similar recognition profiles for...
The purpose of this study was to quantify the percentage and the mean fluorescence intensity of v... more The purpose of this study was to quantify the percentage and the mean fluorescence intensity of viable alternatively activated monocytes/macrophages (AAMø) CD163+ positive for calcitonin gene-related peptide receptor (CGRPr) within the total AAMø population in human dental pulp. Pulp tissue samples were collected from teeth with a clinical diagnosis of irreversible pulpitis (n = 13), pulps with induced inflammation (n = 13), and normal pulps (n = 13). All samples were labeled to identify positive cells for CGRPr and CD163 using a flow cytometry assay. Results demonstrated that a high percentage of total viable AAMø CD163+ expressed CGRPr on their membranes (72.12% in healthy pulp, 62.20% in irreversible pulpitis, and 58.01% in induced pulpitis). Significant differences were found between mean AAMø CD163+ fluorescence for CGRPr according to pulp condition, being greater in irreversible pulpitis. It can be concluded that AAMø CD163+ are expressed during normal and inflammatory process...
The purpose of this study was to quantify the percentage and the mean fluorescence intensity of v... more The purpose of this study was to quantify the percentage and the mean fluorescence intensity of viable alternatively activated monocytes/macrophages (AAMø) CD163+ positive for calcitonin gene-related peptide receptor (CGRPr) within the total AAMø population in human dental ...
Understanding pulp repair and regeneration requires being familiar with this tissue's behavi... more Understanding pulp repair and regeneration requires being familiar with this tissue's behavior under extreme conditions, such as postmortem state where an abrupt interruption of tissue blood supply occurs. The purpose of this study was to quantify cell viability and the amount of ...
Insulin-like growth factor-1 (IGF-1) plays an important role in cell proliferation and differenti... more Insulin-like growth factor-1 (IGF-1) plays an important role in cell proliferation and differentiation. The purpose of this study was to use a radioreceptor assay to evaluate whether IGF-1 receptors are present in human pulp and to determine whether differences in its ...
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