Some smooth muscle relaxant drugs devoid of anticholinergic action have been tested for their int... more Some smooth muscle relaxant drugs devoid of anticholinergic action have been tested for their interaction with calmodulin, calmodulin-stimulated cyclic nucleotide phosphodiesterase activity, and uterine membrane binding sites for nitrendipine and adenosine. The myolytic activity of octylonium bromide and pinaverium bromide may be due to their interaction with calmodulin-dependent systems. Trimebutine maleate does not bind either to calmodulin or to nitrendipine and adenosine receptors. Tiropramide has no effect on calmodulin-dependent systems and on Ca2+ channels but it shows a competition for the A2-type adenosine receptors.
The ability of polyamines to interact with biological membranes was first described by Tabor who ... more The ability of polyamines to interact with biological membranes was first described by Tabor who showed that polyamines aggregate subcellular organelles and stabilize mitochondria and protoplasts (1). Since then many reports pointed out a possible involvement of polyamines on membrane structure, metabolism and function.
The RM values of naphthols obtained in a chromatographic system where the stationary phase consis... more The RM values of naphthols obtained in a chromatographic system where the stationary phase consisted of a silica gel G layer impregnated with silicone oil are much more closely related to the log P values in an octanol-water system than the RM values determined on polyamide layers. Similarly, the RM values of a series of acetophenones in the silicone system are closely related to their log P values. The equations describing the structure-activity relationship indicate the importance of lipophilic character and halogen substitution in determining the hemolytic activity and the acute toxicity of compounds.
... The series of 'Corresponding author: Gabriele Hakim. ... LOOH Determination The LOOH con... more ... The series of 'Corresponding author: Gabriele Hakim. ... LOOH Determination The LOOH content of the liposomes was deter-mined with the thiocyanate method1221 as described by Cavallini eta1.W The liposomes (150 pg phospholipid) were dissolved in 3 ml of glacial ...
Applied Biochemistry and Biotechnology, Sep 1, 1981
A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransfera... more A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjugates.The new adsorbent gives comparable yields and purifications with those obtained by ligands similar to the nucleotide moiety of the substrate and to the "specifier" protein, α-lactalbumin.The soluble galactosyltransferase from rat ventral prostate is effectively removed from the high speed supernatant by an ovomucoid-Sepharose column. The enzyme can be eluted with buffer containing EDTA andN-acetylglucosamine in a high yield (75-80%) and in a purified form (4000-fold purification). The stability of ovomucoid to heat and to high concentrations of urea and its inhibition of some proteases makes the conjugate easy to operate with an quite useful even with rather crude preparations.
Some smooth muscle relaxant drugs devoid of anticholinergic action have been tested for their int... more Some smooth muscle relaxant drugs devoid of anticholinergic action have been tested for their interaction with calmodulin, calmodulin-stimulated cyclic nucleotide phosphodiesterase activity, and uterine membrane binding sites for nitrendipine and adenosine. The myolytic activity of octylonium bromide and pinaverium bromide may be due to their interaction with calmodulin-dependent systems. Trimebutine maleate does not bind either to calmodulin or to nitrendipine and adenosine receptors. Tiropramide has no effect on calmodulin-dependent systems and on Ca2+ channels but it shows a competition for the A2-type adenosine receptors.
The ability of polyamines to interact with biological membranes was first described by Tabor who ... more The ability of polyamines to interact with biological membranes was first described by Tabor who showed that polyamines aggregate subcellular organelles and stabilize mitochondria and protoplasts (1). Since then many reports pointed out a possible involvement of polyamines on membrane structure, metabolism and function.
The RM values of naphthols obtained in a chromatographic system where the stationary phase consis... more The RM values of naphthols obtained in a chromatographic system where the stationary phase consisted of a silica gel G layer impregnated with silicone oil are much more closely related to the log P values in an octanol-water system than the RM values determined on polyamide layers. Similarly, the RM values of a series of acetophenones in the silicone system are closely related to their log P values. The equations describing the structure-activity relationship indicate the importance of lipophilic character and halogen substitution in determining the hemolytic activity and the acute toxicity of compounds.
... The series of 'Corresponding author: Gabriele Hakim. ... LOOH Determination The LOOH con... more ... The series of 'Corresponding author: Gabriele Hakim. ... LOOH Determination The LOOH content of the liposomes was deter-mined with the thiocyanate method1221 as described by Cavallini eta1.W The liposomes (150 pg phospholipid) were dissolved in 3 ml of glacial ...
Applied Biochemistry and Biotechnology, Sep 1, 1981
A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransfera... more A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjugates.The new adsorbent gives comparable yields and purifications with those obtained by ligands similar to the nucleotide moiety of the substrate and to the "specifier" protein, α-lactalbumin.The soluble galactosyltransferase from rat ventral prostate is effectively removed from the high speed supernatant by an ovomucoid-Sepharose column. The enzyme can be eluted with buffer containing EDTA andN-acetylglucosamine in a high yield (75-80%) and in a purified form (4000-fold purification). The stability of ovomucoid to heat and to high concentrations of urea and its inhibition of some proteases makes the conjugate easy to operate with an quite useful even with rather crude preparations.
Uploads