The mosquito species Aedes (Ochlerotatus) coluzzii Rioux, Guilvard & Pasteur, 1998 was distinguis... more The mosquito species Aedes (Ochlerotatus) coluzzii Rioux, Guilvard & Pasteur, 1998 was distinguished from its sibling species Aedes detritus (Haliday, 1833) using an isoenzymatic method that required the destruction of the entire specimen, therefore no holotype was designated by the species authors. We aimed to designate a neotype for Ae. coluzzii from specimens collected from the type-locality and individually reared up to adult stage. Genomic DNA was extracted from pupal exuvia and ITS2 was sequenced, enabling verification of the identity of each specimen as Ae. coluzzii or Ae. detritus. Among the series of Ae. coluzzii, a male was designated as neotype and deposited in a collection. To our knowledge, this is the first time the type of a mosquito species is deposited thanks to its molecular identification from its pupal exuvia. The set of identified specimens allowed additional phylogenetic and morphologic studies.
Les moustiques (Diptera, Culicidae) forment une famille d’insectes d’une importance considerable ... more Les moustiques (Diptera, Culicidae) forment une famille d’insectes d’une importance considerable en sante publique. Les mentions de leur presence/absence ont ete recherchees dans la litterature et aupres de sites internet specialises pour huit pays d’Afrique : Cap-Vert, Mauritanie, Senegal, Gambie, Mali, Burkina Faso, Niger et Tchad. Au total, 216 especes ont ete repertoriees, appartenant a 13 genres culicidiens : Anopheles (48 especes), Aedeomyia (2), Aedes (62), Coquillettidia (6), Culex (54), Culiseta (1), Eretmapodites (7), Ficalbia (3), Lutzia (1), Mansonia (2), Mimomyia (7), Toxorhynchites (4) et Uranotaenia (19). La presence de ces especes dans la zone d’etude est certaine sauf pour trois especes dont la presence est douteuse. Cette richesse specifique represente 6% de la richesse mondiale. Les pays hebergeant la plus grande richesse specifique sont le Burkina Faso (162 especes), le Senegal (142) et le Mali (110) ; le pays avec la plus faible est le Cap-Vert (11). Cette riche...
Annales de la Société belge de médecine tropicale, 1993
The classical method to estimate the malaria transmission in an endemic area is based on the numb... more The classical method to estimate the malaria transmission in an endemic area is based on the number and infectivity of human landing mosquitoes. To compare this method with CDC Miniature Light Trap a study was carried out in a forested area of South Cameroon. Light-trap and servant were placed in six different houses of the same village from 08 p.m. to 06 a.m. In average the human landing caught 29.9 anopheline females per night, while light-trap 52.4. The ratio of light-trap on human landing was 1.75 for Anopheles genus. This ratio varied with the species: 1.88 for An. nili and 0.54 for An. gambiae s.s. Light-trap showed disadvantages, especially the lack of steadiness and the fact that only 49.6% (n = 238) of potential vectors of malaria were in good standing for dissection of salivary glands. The authors concluded that human landing remained the most efficient method of catching for the estimation of human malaria transmission in the forested area of Africa. Nevertheless, the low...
bioRxiv (Cold Spring Harbor Laboratory), Mar 20, 2022
The ANOSPP amplicon panel is a genus-wide targeted sequencing panel to facilitate large-scale mon... more The ANOSPP amplicon panel is a genus-wide targeted sequencing panel to facilitate large-scale monitoring of Anopheles species diversity. Combining information from the 62 nuclear amplicons present in the ANOSPP panel allows for a more nuanced species assignment than single gene (e.g. COI) barcoding, which is desirable in the light of permeable species boundaries. Here, we present NNoVAE, a method using Nearest Neighbours (NN) and Variational Autoencoders (VAE), which we apply to k-mers resulting from the ANOSPP amplicon sequences in order to hierarchically assign species identity. The NN step assigns a sample to a species-group by comparing the k-mers arising from each haplotype’s amplicon sequence to a reference database. The VAE step is required to distinguish between closely related species, and also has sufficient resolution to reveal population structure within species. In tests on independent samples with over 80% amplicon coverage, NNoVAE correctly classifies to species level 98% of samples within the An. gambiae complex and 89% of samples outside the complex. We apply NNoVAE to over two thousand new samples from Burkina Faso and Gabon, identifying unexpected species in Gabon. NNoVAE presents an approach that may be of value to other targeted sequencing panels, and is a method that will be used to survey Anopheles species diversity and Plasmodium transmission patterns through space and time on a large scale, with plans to analyse half a million mosquitoes in the next five years.
The mosquito species Aedes (Ochlerotatus) coluzzii Rioux, Guilvard & Pasteur, 1998 was distinguis... more The mosquito species Aedes (Ochlerotatus) coluzzii Rioux, Guilvard & Pasteur, 1998 was distinguished from its sibling species Aedes detritus (Haliday, 1833) using an isoenzymatic method that required the destruction of the entire specimen, therefore no holotype was designated by the species authors. We aimed to designate a neotype for Ae. coluzzii from specimens collected from the type-locality and individually reared up to adult stage. Genomic DNA was extracted from pupal exuvia and ITS2 was sequenced, enabling verification of the identity of each specimen as Ae. coluzzii or Ae. detritus. Among the series of Ae. coluzzii, a male was designated as neotype and deposited in a collection. To our knowledge, this is the first time the type of a mosquito species is deposited thanks to its molecular identification from its pupal exuvia. The set of identified specimens allowed additional phylogenetic and morphologic studies.
Les moustiques (Diptera, Culicidae) forment une famille d’insectes d’une importance considerable ... more Les moustiques (Diptera, Culicidae) forment une famille d’insectes d’une importance considerable en sante publique. Les mentions de leur presence/absence ont ete recherchees dans la litterature et aupres de sites internet specialises pour huit pays d’Afrique : Cap-Vert, Mauritanie, Senegal, Gambie, Mali, Burkina Faso, Niger et Tchad. Au total, 216 especes ont ete repertoriees, appartenant a 13 genres culicidiens : Anopheles (48 especes), Aedeomyia (2), Aedes (62), Coquillettidia (6), Culex (54), Culiseta (1), Eretmapodites (7), Ficalbia (3), Lutzia (1), Mansonia (2), Mimomyia (7), Toxorhynchites (4) et Uranotaenia (19). La presence de ces especes dans la zone d’etude est certaine sauf pour trois especes dont la presence est douteuse. Cette richesse specifique represente 6% de la richesse mondiale. Les pays hebergeant la plus grande richesse specifique sont le Burkina Faso (162 especes), le Senegal (142) et le Mali (110) ; le pays avec la plus faible est le Cap-Vert (11). Cette riche...
Annales de la Société belge de médecine tropicale, 1993
The classical method to estimate the malaria transmission in an endemic area is based on the numb... more The classical method to estimate the malaria transmission in an endemic area is based on the number and infectivity of human landing mosquitoes. To compare this method with CDC Miniature Light Trap a study was carried out in a forested area of South Cameroon. Light-trap and servant were placed in six different houses of the same village from 08 p.m. to 06 a.m. In average the human landing caught 29.9 anopheline females per night, while light-trap 52.4. The ratio of light-trap on human landing was 1.75 for Anopheles genus. This ratio varied with the species: 1.88 for An. nili and 0.54 for An. gambiae s.s. Light-trap showed disadvantages, especially the lack of steadiness and the fact that only 49.6% (n = 238) of potential vectors of malaria were in good standing for dissection of salivary glands. The authors concluded that human landing remained the most efficient method of catching for the estimation of human malaria transmission in the forested area of Africa. Nevertheless, the low...
bioRxiv (Cold Spring Harbor Laboratory), Mar 20, 2022
The ANOSPP amplicon panel is a genus-wide targeted sequencing panel to facilitate large-scale mon... more The ANOSPP amplicon panel is a genus-wide targeted sequencing panel to facilitate large-scale monitoring of Anopheles species diversity. Combining information from the 62 nuclear amplicons present in the ANOSPP panel allows for a more nuanced species assignment than single gene (e.g. COI) barcoding, which is desirable in the light of permeable species boundaries. Here, we present NNoVAE, a method using Nearest Neighbours (NN) and Variational Autoencoders (VAE), which we apply to k-mers resulting from the ANOSPP amplicon sequences in order to hierarchically assign species identity. The NN step assigns a sample to a species-group by comparing the k-mers arising from each haplotype’s amplicon sequence to a reference database. The VAE step is required to distinguish between closely related species, and also has sufficient resolution to reveal population structure within species. In tests on independent samples with over 80% amplicon coverage, NNoVAE correctly classifies to species level 98% of samples within the An. gambiae complex and 89% of samples outside the complex. We apply NNoVAE to over two thousand new samples from Burkina Faso and Gabon, identifying unexpected species in Gabon. NNoVAE presents an approach that may be of value to other targeted sequencing panels, and is a method that will be used to survey Anopheles species diversity and Plasmodium transmission patterns through space and time on a large scale, with plans to analyse half a million mosquitoes in the next five years.
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