Stephen Forsythe
After I left Nottingham Trent University in 2017, I remained an independent advisor to UK FSA's 'Advisory Committee for Animal Feedingstuffs' (http://acaf.food.gov.uk/), 'Joint Expert Group', and 'Advisory Committee for Microbiological Safety of Foods-AMR subgroup' (ACMSF-AMR) until Dec 2019. I am currently an advisory board member of the Centre for Food Safety (CFS), Stellenbosch University and visiting Professor to various universities including Hong Kong.
I have 150 peer reviewed publications.
I am curator of Cronobacter PubMLST database (>3000 strains, >1000 genomes with metadata); www.pubmlst.org/cronobacter/.
Food microbiology and neonatal microbiome specialist; conventional and microbial genomics.
Training specialist in food microbiology to industry; conventional and DNA-sequence based methods.
Former Professor of Microbiology and researcher in various aspects of microbiology; applied, food and bioinformatics. The main focus has centred on food-borne pathogens and their control.
Research ranges from primary isolation methods, phenotyping and genotyping characterisation, through to virulence studies using in vitro tissue culture. Current research projects also involve multilocus sequence typing (MLST), bacterial genomics and microbial bioinformatics. Recently we found one lineage (ST4) of Cronobacter was associated with most cases of neonatal meningitis cases.
Address: foodmicrobe.com, Adams Hill, Keyworth, Nottinghamshire, UK. NG12 5GY
I have 150 peer reviewed publications.
I am curator of Cronobacter PubMLST database (>3000 strains, >1000 genomes with metadata); www.pubmlst.org/cronobacter/.
Food microbiology and neonatal microbiome specialist; conventional and microbial genomics.
Training specialist in food microbiology to industry; conventional and DNA-sequence based methods.
Former Professor of Microbiology and researcher in various aspects of microbiology; applied, food and bioinformatics. The main focus has centred on food-borne pathogens and their control.
Research ranges from primary isolation methods, phenotyping and genotyping characterisation, through to virulence studies using in vitro tissue culture. Current research projects also involve multilocus sequence typing (MLST), bacterial genomics and microbial bioinformatics. Recently we found one lineage (ST4) of Cronobacter was associated with most cases of neonatal meningitis cases.
Address: foodmicrobe.com, Adams Hill, Keyworth, Nottinghamshire, UK. NG12 5GY
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Videos by Stephen Forsythe
Please note my email address has changed to sforsythe4j@gmail.com, and my homepage is www.foodmicrobe.com where you can download my papers from the 'Publications' page.
Part 1: Overview
Part 2: Isolates
Part 3: Whole genome MLST
Part 4: Exporting, eBURST, and example analysis
Other parts to follow will cover the analysis of whole genomes.
This video reviews how the identification of the organism has changed over the years, and the confusion that has occurred with related organisms. The video encourages you to check the basis of one's identification scheme and also points out some papers on the organism's pathogenicity, which were actually based on mis-identified organisms.
This is the 5th video in a series on Cronobacter, the others are:
10 year analysis of an outbreak:
Part 1: https://youtu.be/alK_D-Jhh8w Part 2: https://youtu.be/KRG4toJRjBA Michigan isolates: https://youtu.be/A_VmlLN0b30
C. sakazakii CC4 : https://youtu.be/rJO9O3zadBk
Please contact me by email if you need any clarification on this topic; sforsythe4j@gmail.com.
Please note my email address has changed to sforsythe4j@gmail.com, and my homepage is www.foodmicrobe.com where you can download my papers from the 'Publications' page.
Please note my email address has changed to sforsythe4j@gmail.com, and my homepage is www.foodmicrobe.com where you can download my papers from the 'Publications' page.
It will show how progress in DNA sequencing techniques led to the recognition of Cronobacter pathovars such as CC4. It will then show the application of MLST, CRISPR-cas array and SNP analysis with resultant greater and greater ability to distinguish between strains.
Other videos are :
10 year analysis of an outbreak Part 2: https://youtu.be/KRG4toJRjBA
Michigan isolates: https://youtu.be/A_VmlLN0b30
C. sakazakii CC4 : https://youtu.be/rJO9O3zadBk
Please note my email address has changed to sforsythe4j@gmail.com, and my homepage is www.foodmicrobe.com where you can download my papers from the 'Publications' page.
Other videos can be found at :
10 year analysis of an outbreak Part 1: https://youtu.be/alK_D-Jhh8w
Michigan isolates: https://youtu.be/A_VmlLN0b30
C. sakazakii CC4 : https://youtu.be/rJO9O3zadBk
Books by Stephen Forsythe
• Advances in genomic analysis techniques for key organisms, including E. coli, Salmonella, and L. monocytogenes
• Emerging information on high-throughput sequencing and genomic epidemiology based on genomic analysis of isolates
• Recent work on investigations into foodborne infection outbreaks, demonstrating the public health costs of unsafe food production
• Updates to the national and international surveillance systems, including social media
Papers by Stephen Forsythe
Please note my email address has changed to sforsythe4j@gmail.com, and my homepage is www.foodmicrobe.com where you can download my papers from the 'Publications' page.
Part 1: Overview
Part 2: Isolates
Part 3: Whole genome MLST
Part 4: Exporting, eBURST, and example analysis
Other parts to follow will cover the analysis of whole genomes.
This video reviews how the identification of the organism has changed over the years, and the confusion that has occurred with related organisms. The video encourages you to check the basis of one's identification scheme and also points out some papers on the organism's pathogenicity, which were actually based on mis-identified organisms.
This is the 5th video in a series on Cronobacter, the others are:
10 year analysis of an outbreak:
Part 1: https://youtu.be/alK_D-Jhh8w Part 2: https://youtu.be/KRG4toJRjBA Michigan isolates: https://youtu.be/A_VmlLN0b30
C. sakazakii CC4 : https://youtu.be/rJO9O3zadBk
Please contact me by email if you need any clarification on this topic; sforsythe4j@gmail.com.
Please note my email address has changed to sforsythe4j@gmail.com, and my homepage is www.foodmicrobe.com where you can download my papers from the 'Publications' page.
Please note my email address has changed to sforsythe4j@gmail.com, and my homepage is www.foodmicrobe.com where you can download my papers from the 'Publications' page.
It will show how progress in DNA sequencing techniques led to the recognition of Cronobacter pathovars such as CC4. It will then show the application of MLST, CRISPR-cas array and SNP analysis with resultant greater and greater ability to distinguish between strains.
Other videos are :
10 year analysis of an outbreak Part 2: https://youtu.be/KRG4toJRjBA
Michigan isolates: https://youtu.be/A_VmlLN0b30
C. sakazakii CC4 : https://youtu.be/rJO9O3zadBk
Please note my email address has changed to sforsythe4j@gmail.com, and my homepage is www.foodmicrobe.com where you can download my papers from the 'Publications' page.
Other videos can be found at :
10 year analysis of an outbreak Part 1: https://youtu.be/alK_D-Jhh8w
Michigan isolates: https://youtu.be/A_VmlLN0b30
C. sakazakii CC4 : https://youtu.be/rJO9O3zadBk
• Advances in genomic analysis techniques for key organisms, including E. coli, Salmonella, and L. monocytogenes
• Emerging information on high-throughput sequencing and genomic epidemiology based on genomic analysis of isolates
• Recent work on investigations into foodborne infection outbreaks, demonstrating the public health costs of unsafe food production
• Updates to the national and international surveillance systems, including social media
YouTube URL: https://youtu.be/tWiPPPgsTco
BTW: The Excel spreadsheet which is generated has various TABS, including listing genes in common and variable, etc. My video was simply a taster and focused on the large amount of data on the first TAB.
The video reviews why C. sakazakii ST/CC4 is regarded as a neonatal meningitic pathovar, and that the CDC MMWR paper was concerning the meningitic pathovar, but it was not mentioned as their paper was based on SNP analysis.
This is the pdf version of a YouTube video that reviews how the identification of the organism has changed over the years, and the confusion that has occurred with related organisms. The video encourages you to check the basis of one's identification scheme and also points out some papers on the organism's pathogenicity, which were actually based on mis-identified organisms.
This is the 5th video in a series on Cronobacter, the others are:
10 year analysis of an outbreak:
Part 1: https://youtu.be/alK_D-Jhh8w Part 2: https://youtu.be/KRG4toJRjBA
Michigan isolates: https://youtu.be/A_VmlLN0b30
C. sakazakii CC4 : https://youtu.be/rJO9O3zadBk
Please contact me by email if you need any clarification on this topic; sforsythe4j@gmail.com.