1. Nilemycin (NM) is found to exert an inhibitory effect on the mouse tumor Sarcoma 180 near toxi... more 1. Nilemycin (NM) is found to exert an inhibitory effect on the mouse tumor Sarcoma 180 near toxic doses but not with Leukemia 1210. 2. In Yoshida rat sarcoma cells NM inhibits cellular de novo nucleic acid synthesis and protein to a much lesser extent. 3. More than 50% inhibition by NM to de novo synthesis of RNA, in a system using calf thymus DNA as a template, could be observed. 4. Suitable levels of NM reduce the S values of DNA. 5. The antibiotic induced metachromatic changes in the u.v. spectrum of DNA solutions. 6. NM markedly inhibited the polynucleotide ligase repairing action on DNase-1-nicked DNA. 7. It is presumed that NM intercalates nicked DNA into such a configuration that the reactive sites of the polynucleotides are inaccessible to the ligase activities.
Euphorbia dendroides can act as a free radical scavenger, so it protects against acute hepatic da... more Euphorbia dendroides can act as a free radical scavenger, so it protects against acute hepatic damage induced by CCl 4 in rats. Also, it increases the Tm of salmon testis DNA from 68°C to about 81°C. In addition to that, it has shown good antibacterial effect against S. aureus, E. coli, B. subtilis and antifungal effect against A. niger. Along with showing good anticancer effects against HepG2 cells with IC 50 = 9.5 mg/mL, HCT-116 cells with IC 50 = 13.6 mg/mL and MCF-7 with IC 50 = 20.9 mg/mL. The docking study was carried out to explore how genistein, the main compound isolated from the extract of E. dendroides, binds to both VEGFR-2 and DNA active sites. Additionally, our compound displayed very well in Silico predicted ADMET profile. Genistein has better absorption (92.893) compared to sorafenib (84.731) and doxorubicin (62.37), as well as not showing any hepatotoxic effects.
A considerable increase in the prevalence of metabolic syndrome (MS) has been reported in paralle... more A considerable increase in the prevalence of metabolic syndrome (MS) has been reported in parallel to the increasing frequency of childhood obesity and type 2 diabetes mellitus. This study provides current estimates of the metabolic syndrome and some of its individual components in obese Egyptian adolescents. A total of 93 persons were submitted to this study and were classified into two groups. The first one included 53 obese patients without MS. Second group included 40 obese patients with MS. Both were compared with control group (40 healthy persons). All were cross matched regarding age, sex and race. The studied subjects were investigated for serum C-reactive protein (CRP), adiponectin and homocysteine (Hcy). In addition, some lipid parameters via total cholesterol (TC), high density lipoprotein (HDL-c), low density lipoprotein (LDL-c) and triacylglycerol (TAG) were also determined to reflect the presence of dyslipidemia. Our study showed that there was a highly significant ele...
The Journal of the Egyptian Public Health Association, 1998
The magnitude of Sexually Transmitted Diseases (STD) in the Eastern Mediterranean Region is not e... more The magnitude of Sexually Transmitted Diseases (STD) in the Eastern Mediterranean Region is not exactly known but considered to be not insignificant. A realistic estimate of the problem is necessary, to design an effective control program. This present study aimed to identify the most prevalent sexually transmitted viral agents among patients attending the STD clinic at Cairo Hospital for Skin and Venereal Diseases (El-Haud El-Marsoud). From persons attending this clinic whether complaining or visiting for screening, between October 1993 to April 1995, 95 cases were studied by history taking, clinical examination, bacteriological and serological examination for the detection of the most prevalent sexually transmitted organisms. Certain demographic and socio-economic factors interacting with sexual attitudes and practices were also studied. All the collected data for each patient were recorded. Antibodies (IgG) to cytomegalovirus (CMV) were detected in 86.3%, HBsAg in 10.5%, and anti...
Introduction: Epilepsy is the most common chronic neurological disorders, distinguished by recurr... more Introduction: Epilepsy is the most common chronic neurological disorders, distinguished by recurrent seizures of cerebral origin. The disease affects more than 70 million people worldwide. Epilepsy usually requires long-term antiepileptic drugs (AEDs) including valporic acid (VPA). It was reported that, long term VPA therapy started to be associated with low bone mineral density and increase in fracture risk and abnormalities in bone metabolism. L-carnitine is an amino acid that is produced in the body naturally. L-carnitine displays a direct effect on increasing osteoblast activity and proliferation, as well as the expression of collagen type I and bone osteopontinObjective: The target of this work is to estimate the effect of valproic acid on bone and the possible protective effect of L-carnitine administration in adult male guinea pigs.Materials & Methods: Forty adult male guinea pigs were utilized in the current study. The guinea pigs were divided into four groups, Group I (cont...
International Journal of Research in Medical Sciences
Background: Familial Mediterranean fever (FMF) is an autosomal recessive disease mainly affecting... more Background: Familial Mediterranean fever (FMF) is an autosomal recessive disease mainly affecting subjects of the Mediterranean origin. It is an auto-inflammatory periodic disorder that is caused by mutations in the Mediterranean fever gene (MEFV) located on chromosome 16.Methods: The current study was designed to assess the prevalence and frequency of different MEFV gene mutations among 104 FMF clinically diagnosed Egyptian patients and to evaluate the change extent in the values of some biochemical markers (ESR, CRP, Fibrinogen-C, SAA and IL1) in different participants with different FMF severity scores.Results: According to allele status 28 patients (27%) were homozygous mutation carriers, 38 (36.5%) were with compound heterozygous mutations and 38 (36.5%) were identified as heterozygous for one of the studied mutations. Of the studied mutations, M694I, E148Q, V726A, M680I, and M694V accounted for 28.1%, 26.8%, 16.9%, and 11.3% of mutations respectively. The R761H and P369S mutat...
Although a clear mechanism underlying the pathophysiology of schizophrenia (SZ) remains elusive, ... more Although a clear mechanism underlying the pathophysiology of schizophrenia (SZ) remains elusive, oxidative stress, inflammatory syndrome and immune activation have become an attractive hypothesis for explaining the pathophysiology of SZ. Data from prior studies on the role of matrix metalloproteinase 9 (MMP-9) and brain-derived neurotrophic factor (BDNF) single nucleotide polymorphisms (SNPs) in SZ are contradictory. We aimed to investigate whether oxidative stress, inflammatory and immune activation markers as well as MMP-9 levels may be implicated in SZ pathogenesis. The association of MMP-9 and BDNF SNPs with the clinical expression of SZ was examined. Ninety-four subjects were recruited, including 44 SZ patients and 50 healthy controls. Serum levels of thiobarbituric acid reactive substances (TBARS), protein carbonyl content (PCC), nitrite, C-reactive protein (CRP), interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α), Beta-2 microglobulin (Β2M), complement component 3 (C3)...
Despite their undoubted helpfulness in diagnosing sepsis, increased blood C-reactive protein (CRP... more Despite their undoubted helpfulness in diagnosing sepsis, increased blood C-reactive protein (CRP) and procalcitonin (PCT) levels have been described in many noninfectious conditions. Presepsin is a soluble fragment of the cluster of differentiation 14 involved in pathogen recognition by innate immunity. We aimed to investigate the diagnostic and prognostic performance of presepsin in comparison to PCT and CRP in patients presenting with systemic inflammatory response syndrome (SIRS) and suspected sepsis. Seventy-six subjects were enrolled in this study, including 51 patients with SIRS as well as 25 healthy subjects. Plasma presepsin, PCT and CRP levels were serially measured on admission and at days 1, 3, 7 and 15. Presepsin and PCT yielded similar diagnostic accuracy, whereas presepsin performed significantly better than CRP. Presepsin and PCT showed comparable performance for predicting 28-day mortality, and both biomarkers performed significantly better than CRP. In septic patients, presepsin revealed earlier concentration changes over time when compared to PCT and CRP. Presepsin and PCT could differentiate between septic and non-septic patients with comparable accuracy and both biomarkers showed similar performance for predicting 28-day mortality. Early changes in presepsin concentrations might reflect the appropriateness of the therapeutic modality and could be useful for making effective treatment decisions.
1. Nilemycin (NM) is found to exert an inhibitory effect on the mouse tumor Sarcoma 180 near toxi... more 1. Nilemycin (NM) is found to exert an inhibitory effect on the mouse tumor Sarcoma 180 near toxic doses but not with Leukemia 1210. 2. In Yoshida rat sarcoma cells NM inhibits cellular de novo nucleic acid synthesis and protein to a much lesser extent. 3. More than 50% inhibition by NM to de novo synthesis of RNA, in a system using calf thymus DNA as a template, could be observed. 4. Suitable levels of NM reduce the S values of DNA. 5. The antibiotic induced metachromatic changes in the u.v. spectrum of DNA solutions. 6. NM markedly inhibited the polynucleotide ligase repairing action on DNase-1-nicked DNA. 7. It is presumed that NM intercalates nicked DNA into such a configuration that the reactive sites of the polynucleotides are inaccessible to the ligase activities.
Euphorbia dendroides can act as a free radical scavenger, so it protects against acute hepatic da... more Euphorbia dendroides can act as a free radical scavenger, so it protects against acute hepatic damage induced by CCl 4 in rats. Also, it increases the Tm of salmon testis DNA from 68°C to about 81°C. In addition to that, it has shown good antibacterial effect against S. aureus, E. coli, B. subtilis and antifungal effect against A. niger. Along with showing good anticancer effects against HepG2 cells with IC 50 = 9.5 mg/mL, HCT-116 cells with IC 50 = 13.6 mg/mL and MCF-7 with IC 50 = 20.9 mg/mL. The docking study was carried out to explore how genistein, the main compound isolated from the extract of E. dendroides, binds to both VEGFR-2 and DNA active sites. Additionally, our compound displayed very well in Silico predicted ADMET profile. Genistein has better absorption (92.893) compared to sorafenib (84.731) and doxorubicin (62.37), as well as not showing any hepatotoxic effects.
A considerable increase in the prevalence of metabolic syndrome (MS) has been reported in paralle... more A considerable increase in the prevalence of metabolic syndrome (MS) has been reported in parallel to the increasing frequency of childhood obesity and type 2 diabetes mellitus. This study provides current estimates of the metabolic syndrome and some of its individual components in obese Egyptian adolescents. A total of 93 persons were submitted to this study and were classified into two groups. The first one included 53 obese patients without MS. Second group included 40 obese patients with MS. Both were compared with control group (40 healthy persons). All were cross matched regarding age, sex and race. The studied subjects were investigated for serum C-reactive protein (CRP), adiponectin and homocysteine (Hcy). In addition, some lipid parameters via total cholesterol (TC), high density lipoprotein (HDL-c), low density lipoprotein (LDL-c) and triacylglycerol (TAG) were also determined to reflect the presence of dyslipidemia. Our study showed that there was a highly significant ele...
The Journal of the Egyptian Public Health Association, 1998
The magnitude of Sexually Transmitted Diseases (STD) in the Eastern Mediterranean Region is not e... more The magnitude of Sexually Transmitted Diseases (STD) in the Eastern Mediterranean Region is not exactly known but considered to be not insignificant. A realistic estimate of the problem is necessary, to design an effective control program. This present study aimed to identify the most prevalent sexually transmitted viral agents among patients attending the STD clinic at Cairo Hospital for Skin and Venereal Diseases (El-Haud El-Marsoud). From persons attending this clinic whether complaining or visiting for screening, between October 1993 to April 1995, 95 cases were studied by history taking, clinical examination, bacteriological and serological examination for the detection of the most prevalent sexually transmitted organisms. Certain demographic and socio-economic factors interacting with sexual attitudes and practices were also studied. All the collected data for each patient were recorded. Antibodies (IgG) to cytomegalovirus (CMV) were detected in 86.3%, HBsAg in 10.5%, and anti...
Introduction: Epilepsy is the most common chronic neurological disorders, distinguished by recurr... more Introduction: Epilepsy is the most common chronic neurological disorders, distinguished by recurrent seizures of cerebral origin. The disease affects more than 70 million people worldwide. Epilepsy usually requires long-term antiepileptic drugs (AEDs) including valporic acid (VPA). It was reported that, long term VPA therapy started to be associated with low bone mineral density and increase in fracture risk and abnormalities in bone metabolism. L-carnitine is an amino acid that is produced in the body naturally. L-carnitine displays a direct effect on increasing osteoblast activity and proliferation, as well as the expression of collagen type I and bone osteopontinObjective: The target of this work is to estimate the effect of valproic acid on bone and the possible protective effect of L-carnitine administration in adult male guinea pigs.Materials & Methods: Forty adult male guinea pigs were utilized in the current study. The guinea pigs were divided into four groups, Group I (cont...
International Journal of Research in Medical Sciences
Background: Familial Mediterranean fever (FMF) is an autosomal recessive disease mainly affecting... more Background: Familial Mediterranean fever (FMF) is an autosomal recessive disease mainly affecting subjects of the Mediterranean origin. It is an auto-inflammatory periodic disorder that is caused by mutations in the Mediterranean fever gene (MEFV) located on chromosome 16.Methods: The current study was designed to assess the prevalence and frequency of different MEFV gene mutations among 104 FMF clinically diagnosed Egyptian patients and to evaluate the change extent in the values of some biochemical markers (ESR, CRP, Fibrinogen-C, SAA and IL1) in different participants with different FMF severity scores.Results: According to allele status 28 patients (27%) were homozygous mutation carriers, 38 (36.5%) were with compound heterozygous mutations and 38 (36.5%) were identified as heterozygous for one of the studied mutations. Of the studied mutations, M694I, E148Q, V726A, M680I, and M694V accounted for 28.1%, 26.8%, 16.9%, and 11.3% of mutations respectively. The R761H and P369S mutat...
Although a clear mechanism underlying the pathophysiology of schizophrenia (SZ) remains elusive, ... more Although a clear mechanism underlying the pathophysiology of schizophrenia (SZ) remains elusive, oxidative stress, inflammatory syndrome and immune activation have become an attractive hypothesis for explaining the pathophysiology of SZ. Data from prior studies on the role of matrix metalloproteinase 9 (MMP-9) and brain-derived neurotrophic factor (BDNF) single nucleotide polymorphisms (SNPs) in SZ are contradictory. We aimed to investigate whether oxidative stress, inflammatory and immune activation markers as well as MMP-9 levels may be implicated in SZ pathogenesis. The association of MMP-9 and BDNF SNPs with the clinical expression of SZ was examined. Ninety-four subjects were recruited, including 44 SZ patients and 50 healthy controls. Serum levels of thiobarbituric acid reactive substances (TBARS), protein carbonyl content (PCC), nitrite, C-reactive protein (CRP), interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-α), Beta-2 microglobulin (Β2M), complement component 3 (C3)...
Despite their undoubted helpfulness in diagnosing sepsis, increased blood C-reactive protein (CRP... more Despite their undoubted helpfulness in diagnosing sepsis, increased blood C-reactive protein (CRP) and procalcitonin (PCT) levels have been described in many noninfectious conditions. Presepsin is a soluble fragment of the cluster of differentiation 14 involved in pathogen recognition by innate immunity. We aimed to investigate the diagnostic and prognostic performance of presepsin in comparison to PCT and CRP in patients presenting with systemic inflammatory response syndrome (SIRS) and suspected sepsis. Seventy-six subjects were enrolled in this study, including 51 patients with SIRS as well as 25 healthy subjects. Plasma presepsin, PCT and CRP levels were serially measured on admission and at days 1, 3, 7 and 15. Presepsin and PCT yielded similar diagnostic accuracy, whereas presepsin performed significantly better than CRP. Presepsin and PCT showed comparable performance for predicting 28-day mortality, and both biomarkers performed significantly better than CRP. In septic patients, presepsin revealed earlier concentration changes over time when compared to PCT and CRP. Presepsin and PCT could differentiate between septic and non-septic patients with comparable accuracy and both biomarkers showed similar performance for predicting 28-day mortality. Early changes in presepsin concentrations might reflect the appropriateness of the therapeutic modality and could be useful for making effective treatment decisions.
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Papers by Fahmy Ali