Exposure to chemical substances has always been a matter of concern for the scientific community.... more Exposure to chemical substances has always been a matter of concern for the scientific community. During the last few years, researchers have been focusing on studying the effects resulting from combined exposure to different substances. In this study, we aimed to determine the DNA damage caused after chronic and combined exposure to substances characterized as endocrine disruptors using comet and micronuclei assays, specifically glyphosate (pure and commercial form), bisphenol A, parabens (methyl-, propyl- and butylparaben), triclosan and bis (2-ethylhexyl) phthalate. The highest mean tail intensity was observed in the group exposed to a high-dose (10 × ADI) mixture of substances (Group 3), with a mean value of 11.97 (11.26–13.90), while statistically significant differences were noticed between the groups exposed to low-dose (1 × ADI) (Group 2) and high-dose (10 × ADI) (Group 3) mixtures of substances (p = 0.003), and between Group 3 and both groups exposed to high doses (10 × ADI...
The extensive use of antibiotics in livestock farming poses increased concerns for human health a... more The extensive use of antibiotics in livestock farming poses increased concerns for human health as residues of these substances are present in edible tissues. The aim of this study was the determination of the levels of four groups of antibiotics (sulfonamides—SAs, tetracyclines—TCs, streptomycines—STr and quinolones—QNLs) in meat samples (muscles, livers and kidneys from beef, chicken and pork) and the estimation of the dietary exposure to antibiotics from meat consumption and the potential hazard for human health. Fifty-four samples of raw meat were randomly collected in 2018 from the Cretan market, Greece and analyzed both with an enzyme-linked immunosorbent assay (ELISA) and liquid chromatography–mass spectrometry (LC–MS). According to the results derived from the ELISA method, only 2% of the meat samples were free from antibiotics, 2% were detected with 4 antibiotics and the great majority of the samples (87%) were detected with 2 to 3 antibiotics. SAs presented the highest det...
Toxicological Risk Assessment and Multi-System Health Impacts from Exposure, 2021
Abstract Telomeres are composites of tandem repeats of a double-stranded DNA sequence coated by t... more Abstract Telomeres are composites of tandem repeats of a double-stranded DNA sequence coated by the shelterin proteins, formed at chromosomal termini, which serve a protective role against DNA degradation and recombination. Telomerase activity is insufficient to prevent telomere shortening with each cell division, and after a critical threshold, proliferation is arrested leading to apoptosis. Telomere length (TL) can be used not only as an aging biomarker but is also indicative of age-related diseases and other health conditions including cancer, cardiovascular diseases, and diabetes, as well as behaviors such as smoking and drug abuse. Although telomeric length is heritable, telomere shortening has been associated with numerous xenobiotics and bioactive compounds with telomerase regulating properties, acting either as activators or inhibitors. Endocrine disrupting chemicals have been found to positively or inversely affect TL following exposure even from early developmental stages reflecting in utero exposures up to adulthood. Nutrient and vitamin intake has been positively correlated to longer TL in epidemiological and experimental studies, while a “whole diet” effect on TL is increasingly being supported. More epidemiological studies in order to build weight of evidence during critical windows of exposure from intrauterine to adulthood are necessary, for coetaneous exposures to the exposome.
Exposure to chemical substances has always been a matter of concern for the scientific community.... more Exposure to chemical substances has always been a matter of concern for the scientific community. During the last few years, researchers have been focusing on studying the effects resulting from combined exposure to different substances. In this study, we aimed to determine the DNA damage caused after chronic and combined exposure to substances characterized as endocrine disruptors using comet and micronuclei assays, specifically glyphosate (pure and commercial form), bisphenol A, parabens (methyl-, propyl- and butylparaben), triclosan and bis (2-ethylhexyl) phthalate. The highest mean tail intensity was observed in the group exposed to a high-dose (10 × ADI) mixture of substances (Group 3), with a mean value of 11.97 (11.26–13.90), while statistically significant differences were noticed between the groups exposed to low-dose (1 × ADI) (Group 2) and high-dose (10 × ADI) (Group 3) mixtures of substances (p = 0.003), and between Group 3 and both groups exposed to high doses (10 × ADI...
The extensive use of antibiotics in livestock farming poses increased concerns for human health a... more The extensive use of antibiotics in livestock farming poses increased concerns for human health as residues of these substances are present in edible tissues. The aim of this study was the determination of the levels of four groups of antibiotics (sulfonamides—SAs, tetracyclines—TCs, streptomycines—STr and quinolones—QNLs) in meat samples (muscles, livers and kidneys from beef, chicken and pork) and the estimation of the dietary exposure to antibiotics from meat consumption and the potential hazard for human health. Fifty-four samples of raw meat were randomly collected in 2018 from the Cretan market, Greece and analyzed both with an enzyme-linked immunosorbent assay (ELISA) and liquid chromatography–mass spectrometry (LC–MS). According to the results derived from the ELISA method, only 2% of the meat samples were free from antibiotics, 2% were detected with 4 antibiotics and the great majority of the samples (87%) were detected with 2 to 3 antibiotics. SAs presented the highest det...
Toxicological Risk Assessment and Multi-System Health Impacts from Exposure, 2021
Abstract Telomeres are composites of tandem repeats of a double-stranded DNA sequence coated by t... more Abstract Telomeres are composites of tandem repeats of a double-stranded DNA sequence coated by the shelterin proteins, formed at chromosomal termini, which serve a protective role against DNA degradation and recombination. Telomerase activity is insufficient to prevent telomere shortening with each cell division, and after a critical threshold, proliferation is arrested leading to apoptosis. Telomere length (TL) can be used not only as an aging biomarker but is also indicative of age-related diseases and other health conditions including cancer, cardiovascular diseases, and diabetes, as well as behaviors such as smoking and drug abuse. Although telomeric length is heritable, telomere shortening has been associated with numerous xenobiotics and bioactive compounds with telomerase regulating properties, acting either as activators or inhibitors. Endocrine disrupting chemicals have been found to positively or inversely affect TL following exposure even from early developmental stages reflecting in utero exposures up to adulthood. Nutrient and vitamin intake has been positively correlated to longer TL in epidemiological and experimental studies, while a “whole diet” effect on TL is increasingly being supported. More epidemiological studies in order to build weight of evidence during critical windows of exposure from intrauterine to adulthood are necessary, for coetaneous exposures to the exposome.
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