Papers by El Mousadik Abdelhamid
Systematic and Applied Acarology, 2021
Citrus brown mite, Eutetranychus orientalis (Klein), known as major pest of citrus crops in diffe... more Citrus brown mite, Eutetranychus orientalis (Klein), known as major pest of citrus crops in different countries, was considered as a quarantine pest in Morocco. This pest is currently among the most potential enemies of Moroccan arboriculturists following the increasing ineffectiveness of the majority of acaricides used in conventional orchards. The present study was designed to collect and morphologically identify specimens from 31 orchards in the five main production regions of Morocco; to study the population dynamics of this pest in Taroudant as well as the molecular identification of a Phytoseiid considered as a potential predator using the mitochondrial 12S rRNA gene. Morphological description confirmed that the collected adult females from all orchards in the five regions belong to E. orientalis. Thus, this exotic pest has invaded all citrus growing regions since its introduction. In the Souss region, two significant peaks of population outbreaks of this pest were observed. A...
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Journal of Entomology, Apr 15, 2020
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Journal of Entomology, Apr 15, 2020
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VIII International Symposium on Artichoke, Cardoon and their Wild Relatives, 2013
ABSTRACT Growing demand for plants of globe artichoke (Cynara cardunculus var. scolymus L.) made ... more ABSTRACT Growing demand for plants of globe artichoke (Cynara cardunculus var. scolymus L.) made it necessary to find a rapid method of multiplication for this plant. In vitro micropropagation is an alternative procedure for obtaining healthy, high quality and uniform clones, important to increase the cultivation area of this species. The contamination problem and low in vitro multiplication rate still remain the limiting factors. The objective of this study is to obtain healthy plants of globe artichoke via in vitro culture with a high multiplication rate. In this work, we report an efficient protocol for in vitro multiplication of globe artichoke using ‘decapitated’ plantlets (removal of apical buds, leaves and roots) and axillary shoots as explants for multiple bud induction. The size of shoots that may control shoot induction was studied. To establish the in vitro culture, plants derived from disinfected seeds were cultivated on MS medium containing 1 mg/L IBA and 0.1 mg/L GA3, followed by their decapitation and subculture on proliferation medium containing 1 mg/L kinetin and 0.1 mg/L NAA. In order to study the influence of explant size on shoot proliferation, four shoots size categories (<1 cm, 1 to 1.5 cm, 1.5 to 2 cm and >2 cm) were evaluated. The results show that decapitation of seedlings influenced the efficiency of shoot induction. The greatest propagation ratio (shoot proliferation) was obtained with ‘decapitated’ explants (17 shoots per explant). The multiplication rate obtained in this work over twelve generations was 7.56. Statistically significant differences were observed for proliferation among the four shoot size categories and the highest rate obtained with those of 1-1.5 cm in size.
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Notulae Botanicae Horti Agrobotanici Cluj-Napoca
A collection of thirty argan trees (Argania spinosa (L.) Skeels), representing the Aoulouz proven... more A collection of thirty argan trees (Argania spinosa (L.) Skeels), representing the Aoulouz provenance in southwest of Morocco were used to study genetic variability and selection for three years. In this study, the genetic diversity of thirty genotypes (tree mothers) of argan (Argania spinosa) collected from Aoulouz was evaluated using agro-morphological characters. The main objective of the study was to assess and describe with multivariate analysis the genetic diversity in order to select good candidate trees for a future breeding program. The results obtained showed a large variation for all the traits examined. Analysis of variance using general linear model provided a significant variation between genotypes. Furthermore, genotypic and phenotypic variances for quantitative traits, particularly for seed length, seed width, almond length and oil content were higher. Phenotypic coefficient of variation was higher than genotypic coefficient of variation for all the characters. High ...
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International Journal of Agriculture and Forestry, 2014
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European Journal of Scientific Research
An efficient protocol for in vitro multiplication of globe artichoke (Cynara cardunculus var. sco... more An efficient protocol for in vitro multiplication of globe artichoke (Cynara cardunculus var. scolymus L.) accession Art 21 was developed using axillary buds obtained from seedlings derived from embryos. A high regeneration efficiency (7.56 shoots/explants) was achieved with axillary buds on a proliferation medium containing 1 mg/l kinetin and 0.1 mg/l NAA after removal of apical buds, leaves and roots from seedlings. Decreasing light intensity from 40 to 20 μE.m -2 .s -1 and reducing explant density from 6-7 to 3-4 shoots per 132 cm 2 of culture medium increased the rate of multiplication by two-fold. Statistically significant differences were observed for proliferation among four shoot size categories (< 1 cm, 1 to 1.5 cm, 1.5 to 2 cm and > 2 cm) with the highest rate obtained with those of 1-1.5 and 1.5-2 cm in size. To our knowledge, this is the first report on the micropropagation of a Moroccan globe artichoke accession. Improved in Vitro Micropropagation of Artichoke (Cy...
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DESCRIPTION Genetic variation in 150 trees of A. spinosa was estimated using inter-simple sequenc... more DESCRIPTION Genetic variation in 150 trees of A. spinosa was estimated using inter-simple sequence repeat (ISSR). The plant material used in this study were originated from five provenances belonging to the principal provinces of argan ecosystem in southwest of Morocco. Based on the nine ISSR primers, 86 loci were scored. At the population level, percentage of polymorphic bands (PPB) was ranged from 75.58 to 82.56 %, Nei’s genetic diversity index (H) ranged from 0.30 to 0.34, Shannon’s information index (I) ranged from 0.44 to 0.49. Analyses of spatial genetic structure using UPGMA, the principal coordinate analysis and Bayesian-based approach from Structure program, showed that the entire provenances were successfully assigned to three groups. There was no significant correlation between genetic distance and geographic distance by Mantel test (r = 0.026, P = 0.942). The genetic differentiation coefficient (Gst = 0.22) was high, it was similar to the result of the analysis of molecu...
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VII International Congress on Cactus Pear and Cochineal, 2013
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Plant-parasitic nematodes affect significantly the production of susceptible plants, including ol... more Plant-parasitic nematodes affect significantly the production of susceptible plants, including olive trees. Knowing that plant production depends on parasite diversity, more information about plant-parasitic nematode communities and about interactions with their habitat is a prerequisite for the development of any control strategy. In this context, nematode communities were determined in soil samples collected from 23 olive growing sites in the Haouz and Souss regions (southern Morocco). These sites corresponded to various modalities: wild (Olea europaea L. ssp. maroccana) (Olea. europaea L. ssp. europaea ), traditional or high-density cropping, rainfed or irrigated,). Ten nematode families and 14 genera were identified, especially spiral nematodes (Helicotylenchus spp. and Rotylenchus spp.), lesion and root-knot nematodes (Pratylenchus spp.) and (Meloidogyne spp.) respectively. Land-use changes (wild vs cropped olive), irrigation and soil physic-chemical characteristics (e.g. pH, t...
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Objectives Plant-parasitic nematodes (PPN) are everywhere present in communities. Community patte... more Objectives Plant-parasitic nematodes (PPN) are everywhere present in communities. Community patterns are used as bio-indicators for plant-production. But at the same time, plant-parasitic nematodes are one of the main biotic stresses on crops. Then, considering the new cultivation programs pushing olive production with high technologies in Morocco ('Green Morocco program') and the enhancement of rooted cutting production, nurseries established in southern Morocco were surveyed in order to analyse the associated PPN communities and the contribution of olive varieties and soil substrates on their diversity. Materials and methods Twenty-two commercial olive nurseries (Olea europaea) located in the Haouz and Souss-Massa-Draa regions in Morocco were selected for nematode surveys conducted in 2013 (Fig. 1). In each nursery surveyed, five rooted cuttings (as replicates) of each variety were randomly sampled (Fig. 2) , and then brought and kept under controlled greenhouse conditions...
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Allozyme diversity of natural and introduced cypress stands in Morocco: consequences for manageme... more Allozyme diversity of natural and introduced cypress stands in Morocco: consequences for management of genetic resources. For the purpose of managing Cupressus atlantica natural genetic resources in Morocco, diversity among 9 provenances from 3 cypress species (Cupressus atlantica and 2 introduced species: C. sempervirens and C. arizonica) was studied using seven enzymatic systems. Mean allele number per locus (A) ranged from 2.0 in C. atlantica and C. arizonica to 1.7 in C. sempervirens. Genetic diversity was high (He = 0.29 in C. atlantica; 0.27 in C. sempervirens and 0.33 in C. arizonica) but heterozygote deficiencies were observed. C. atlantica provenances exhibited a low differentiation (GST = 0.013). The high interspecific differentiation (GST = 0.279) was due to the distance between the 2 Mediterranean cypresses (C. atlantica and C. sempervirens) and the Arizonica cypress. Two enzymatic systems (GOT et SOD) strictly differentiated these 2 origins and could be used for introgr...
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Journal of Colloid Science and Biotechnology, 2014
ABSTRACT This study aims to functionalize cotton textile fibers with bioactive natural oil in ord... more ABSTRACT This study aims to functionalize cotton textile fibers with bioactive natural oil in order to prepare medical textile with induced antifungal activity. A simple and eco-friendly procedure of microencapsulation in aqueous medium was implemented through the use of two natural and biodegradable materials: Thymus leptobotrys essential oil (bioactive natural oil) and Arabic gum (stabilizing agent). Essential oil microcapsule colloidal particle having a particle-size of 1 μm were prepared. The obtained particles dispersion was used for activation of the cotton textile by simple impregnation method. Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy analysis (FTIR) confirmed the fixation of essential oil microcapsules on cotton textile fibers. Antifungal activity (A) of the functionalized textile against Aspergillus Niger ATCC 16404 was evaluated according to the ISO 13629-2 using plate count method. The determination of viable number of spores on cotton textile fibers was monitored daily for one week. From the first day, a significant decrease in spore&#39;s population was detected on the treated textile, as compared with the untreated (control) one. In addition, the study indicated a remarkable antifungal activity (A) growing constantly until reaching the value of 7.5 at the seventh day. More interestingly, the value of (A) was stable during the first three months (more at least than 4), which proof the long term antifungal activity after activation. These promising results make textile processing usable in several areas, especially those related to hygiene in the medical and food industry domains.
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Atlas Journal of Plant Biology, 2011
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Mediterranean Journal of Nutrition and Metabolism, 2013
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Organisms Diversity & Evolution, 2008
... For allozyme electrophoresis we used cellulose acetate plates, applying standard protocols (R... more ... For allozyme electrophoresis we used cellulose acetate plates, applying standard protocols (Richardson et al. 1986; Hebert and Beaton 1993). We analysed a total of 18 allozyme loci (for their electrophoretic conditions, see Habel et al. 2005). Statistics. ...
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Papers by El Mousadik Abdelhamid