Gap junctions are unique intercellular channels that connect the cytoplasm of adjacent cells. The... more Gap junctions are unique intercellular channels that connect the cytoplasm of adjacent cells. They are the only channels that mediate the direct cytoplasmic exchange of small hydrophilic molecules between cells--a process called gap junctional communication. Formed by a family of integral membrane proteins called connexins, gap junctions are dynamic multifunctional complexes that are essential for healthy vertebrate development and physiology. Defects in connexin proteins, and, therefore, in gap junctional communication, are associated with a large variety of pathologies in humans and experimental animals. Thus, knowledge of the molecules that pass through gap junction channels is extremely important. However, aside from some notable cases, the repertoire of biologically important transjunctional molecules remains relatively unexplored. Indeed, the study of the intercellular transfer of endogenous molecules presents formidable challenges. Here we review developments in identifying biologically relevant molecules that pass between cells through gap junction channels.
We employ the DAVE (differential affine velocity estimator, Schuck 2005; 2006) tracking technique... more We employ the DAVE (differential affine velocity estimator, Schuck 2005; 2006) tracking technique on a time series of MDI/1m high spatial resolution line- of-sight magnetograms to measure the photospheric flow velocity for three newly emerging bipolar active regions. We separately calculate the magnetic helicity injection rate of the leading and following polarities to confirm or refute the magnetic helicity asymmetry, found by Tian & Alexander (2009) using MDI/96m low spatial resolution magnetograms. Our results demonstrate that the magnetic helicity asymmetry is robust being present in the three active regions studied, two of which have an observed balance of the magnetic flux. The magnetic helicity injection rate measured is found to depend little on the window size selected, but does depend on the time interval used between the two successive magnetograms tracked. It is found that the measurement of the magnetic helicity injection rate performs well for a window size between 12x10 and 18x15 pixels, and at a time interval {\Delta}t=10 minutes. Moreover, the short-lived magnetic structures, 10-60 minutes, are found to contribute 30-50% of the magnetic helicity injection rate. Comparing with the results calculated by MDI/96m data, we find that the MDI/96m data, in general, can outline the main trend of the magnetic properties, but they significantly underestimate the magnetic flux in strong field region and are not appropriate for quantitative tracking studies, so provide a poor estimate of the amount of magnetic helicity injected into the corona.
There are many ways in which the dose can be expressed in inhalation toxicology studies. This can... more There are many ways in which the dose can be expressed in inhalation toxicology studies. This can lead to confusion when comparing results from studies performed in different laboratories. A working party of the Association of Inhalation Toxicologists has reviewed this subject in detail and has collected data from 10 inhalation laboratories and used these data to determine a new algorithm for the calculation of Respiratory Minute Volume (RMV), one of the most important factors in the calculation of delivered dose. The recommendations of the working party for regulatory inhalation toxicology studies with pharmaceuticals are as follows: 1. The dose should be reported as the delivered dose calculated according to the formula: DD = C x RMV x D(xIF)/BW, where DD = delivered dose (mg/Kg); C = concentration of substance in air (mg/L); RMV =respiratory minute volume or the volume of air inhaled in one minute (L/min); D = duration of exposure (min); IF = proportion by weight of particles that are inhalable by the test species, the inhalable fraction (inclusion of this parameter is not essential provided that the aerosol has reasonable respirability for the intended species. If it is included, the way in which it is determined should be clearly stated); BW = bodyweight (Kg). 2. The RMV for mice, rats, dogs and cynomolgus monkeys should be calculated according to the formula:RMV(L/min) = 0.608 x BW(Kg)(0.852). 3. If deposited dose or the amount of material actually retained inthe respiratory tract is presented as supplementary information,the way in which it is calculated should be clearly stated.4. Dose should always be presented in mg/Kg but may also bepresented in other ways, such as mg/unit body surface area, as supplementary information.
p67 is a lysosomal type I membrane glycoprotein of Trypanosoma brucei. In procyclic stage cells p... more p67 is a lysosomal type I membrane glycoprotein of Trypanosoma brucei. In procyclic stage cells p67 trafficks to the lysosome without modification, but in the bloodstream stage Golgi processing adds poly-N-acetyllactosamine to N-glycans. In both stages proteolytic fragmentation occurs in the lysosome, but turnover is approximately nine times faster in bloodstream cells. Trafficking of wildtype p67 and mutants missing the cytoplasmic (p67DeltaCD) or cytoplasmic/transmembrane domains (p67DeltaTM) was monitored by pulse-chase, surface biotinylation and immunofluorescence. Overexpressed wildtype p67 trafficks normally in procyclics, but some leaks to the cell surface suggesting that the targeting machinery is saturable. p67DeltaCD and p67DeltaTM are delivered to the cell surface and secreted, respectively. The membrane/cytoplasmic domains function correctly in procyclic cells when fused to GFP indicating that these domains are sufficient for stage-specific lysosomal targeting. In contrast, p67 wildtype and deletion reporters are overwhelmingly targeted to the lysosome and degraded in bloodstream cells. These findings suggest that either redundant developmentally regulated targeting signals/machinery are operative in this stage or that the increased endocytic activity of bloodstream cells prevents export of the deletion reporters.
Inertial confinement fusion research at Los Alamos National Laboratory is focused on high-leverag... more Inertial confinement fusion research at Los Alamos National Laboratory is focused on high-leverage areas of thermonuclear ignition to which LANL can apply its historic strengths and that are complementary to high-energy-density-physics topics. Using the Trident and Omega laser facilities, experiments are pursued in laser-plasma instabilities, symmetry, Be technologies, neutron and fusion-product diagnostics, and defect hydrodynamics.
ABSTRACT We present observations from 2007 March 2 of a partial filament eruption characterized b... more ABSTRACT We present observations from 2007 March 2 of a partial filament eruption characterized by two distinct phases of writhing motions: a quasi-static, slowly evolving phase followed by a rapid kinking phase showing a bifurcation of the filament. The quasi-static kinking motions ...
In a recent Letter, Reale & Peres demonstrated that this method can explain the almost isothe... more In a recent Letter, Reale & Peres demonstrated that this method can explain the almost isothermal appearance of T RACE loops (observed by Lenz et al.) as derived from the lter-ratio method. From model-tting of the 171 and 195 uxes of 41 loops, which have loop half-...
Publications of The Astronomical Society of The Pacific, 2007
We describe how the James Webb Space Telescope (JWST) Near-Infrared Spectrograph's (NIRSpec's) de... more We describe how the James Webb Space Telescope (JWST) Near-Infrared Spectrograph's (NIRSpec's) detectors will be read out, and present a model of how noise scales with the number of multiple non-destructive reads sampling-up-the-ramp. We believe that this noise model, which is validated using real and simulated test data, is applicable to most astronomical near-infrared instruments. We describe some non-ideal behaviors that have been observed in engineering grade NIRSpec detectors, and demonstrate that they are unlikely to affect NIRSpec sensitivity, operations, or calibration. These include a HAWAII-2RG reset anomaly and random telegraph noise (RTN). Using real test data, we show that the reset anomaly is: (1) very nearly noiseless and (2) can be easily calibrated out. Likewise, we show that large-amplitude RTN affects only a small and fixed population of pixels. It can therefore be tracked using standard pixel operability maps.
ABSTRACT We report here, for the rst time, on spatial oscillations of coronal loops, which were ... more ABSTRACT We report here, for the rst time, on spatial oscillations of coronal loops, which were detected in extreme-ultraviolet wavelengths (171 with the Transition Region and Coronal Explorer, in the tem-Aé) perature range of MK. The observed loop oscillations occurred ...
Gap junctions are unique intercellular channels that connect the cytoplasm of adjacent cells. The... more Gap junctions are unique intercellular channels that connect the cytoplasm of adjacent cells. They are the only channels that mediate the direct cytoplasmic exchange of small hydrophilic molecules between cells--a process called gap junctional communication. Formed by a family of integral membrane proteins called connexins, gap junctions are dynamic multifunctional complexes that are essential for healthy vertebrate development and physiology. Defects in connexin proteins, and, therefore, in gap junctional communication, are associated with a large variety of pathologies in humans and experimental animals. Thus, knowledge of the molecules that pass through gap junction channels is extremely important. However, aside from some notable cases, the repertoire of biologically important transjunctional molecules remains relatively unexplored. Indeed, the study of the intercellular transfer of endogenous molecules presents formidable challenges. Here we review developments in identifying biologically relevant molecules that pass between cells through gap junction channels.
We employ the DAVE (differential affine velocity estimator, Schuck 2005; 2006) tracking technique... more We employ the DAVE (differential affine velocity estimator, Schuck 2005; 2006) tracking technique on a time series of MDI/1m high spatial resolution line- of-sight magnetograms to measure the photospheric flow velocity for three newly emerging bipolar active regions. We separately calculate the magnetic helicity injection rate of the leading and following polarities to confirm or refute the magnetic helicity asymmetry, found by Tian & Alexander (2009) using MDI/96m low spatial resolution magnetograms. Our results demonstrate that the magnetic helicity asymmetry is robust being present in the three active regions studied, two of which have an observed balance of the magnetic flux. The magnetic helicity injection rate measured is found to depend little on the window size selected, but does depend on the time interval used between the two successive magnetograms tracked. It is found that the measurement of the magnetic helicity injection rate performs well for a window size between 12x10 and 18x15 pixels, and at a time interval {\Delta}t=10 minutes. Moreover, the short-lived magnetic structures, 10-60 minutes, are found to contribute 30-50% of the magnetic helicity injection rate. Comparing with the results calculated by MDI/96m data, we find that the MDI/96m data, in general, can outline the main trend of the magnetic properties, but they significantly underestimate the magnetic flux in strong field region and are not appropriate for quantitative tracking studies, so provide a poor estimate of the amount of magnetic helicity injected into the corona.
There are many ways in which the dose can be expressed in inhalation toxicology studies. This can... more There are many ways in which the dose can be expressed in inhalation toxicology studies. This can lead to confusion when comparing results from studies performed in different laboratories. A working party of the Association of Inhalation Toxicologists has reviewed this subject in detail and has collected data from 10 inhalation laboratories and used these data to determine a new algorithm for the calculation of Respiratory Minute Volume (RMV), one of the most important factors in the calculation of delivered dose. The recommendations of the working party for regulatory inhalation toxicology studies with pharmaceuticals are as follows: 1. The dose should be reported as the delivered dose calculated according to the formula: DD = C x RMV x D(xIF)/BW, where DD = delivered dose (mg/Kg); C = concentration of substance in air (mg/L); RMV =respiratory minute volume or the volume of air inhaled in one minute (L/min); D = duration of exposure (min); IF = proportion by weight of particles that are inhalable by the test species, the inhalable fraction (inclusion of this parameter is not essential provided that the aerosol has reasonable respirability for the intended species. If it is included, the way in which it is determined should be clearly stated); BW = bodyweight (Kg). 2. The RMV for mice, rats, dogs and cynomolgus monkeys should be calculated according to the formula:RMV(L/min) = 0.608 x BW(Kg)(0.852). 3. If deposited dose or the amount of material actually retained inthe respiratory tract is presented as supplementary information,the way in which it is calculated should be clearly stated.4. Dose should always be presented in mg/Kg but may also bepresented in other ways, such as mg/unit body surface area, as supplementary information.
p67 is a lysosomal type I membrane glycoprotein of Trypanosoma brucei. In procyclic stage cells p... more p67 is a lysosomal type I membrane glycoprotein of Trypanosoma brucei. In procyclic stage cells p67 trafficks to the lysosome without modification, but in the bloodstream stage Golgi processing adds poly-N-acetyllactosamine to N-glycans. In both stages proteolytic fragmentation occurs in the lysosome, but turnover is approximately nine times faster in bloodstream cells. Trafficking of wildtype p67 and mutants missing the cytoplasmic (p67DeltaCD) or cytoplasmic/transmembrane domains (p67DeltaTM) was monitored by pulse-chase, surface biotinylation and immunofluorescence. Overexpressed wildtype p67 trafficks normally in procyclics, but some leaks to the cell surface suggesting that the targeting machinery is saturable. p67DeltaCD and p67DeltaTM are delivered to the cell surface and secreted, respectively. The membrane/cytoplasmic domains function correctly in procyclic cells when fused to GFP indicating that these domains are sufficient for stage-specific lysosomal targeting. In contrast, p67 wildtype and deletion reporters are overwhelmingly targeted to the lysosome and degraded in bloodstream cells. These findings suggest that either redundant developmentally regulated targeting signals/machinery are operative in this stage or that the increased endocytic activity of bloodstream cells prevents export of the deletion reporters.
Inertial confinement fusion research at Los Alamos National Laboratory is focused on high-leverag... more Inertial confinement fusion research at Los Alamos National Laboratory is focused on high-leverage areas of thermonuclear ignition to which LANL can apply its historic strengths and that are complementary to high-energy-density-physics topics. Using the Trident and Omega laser facilities, experiments are pursued in laser-plasma instabilities, symmetry, Be technologies, neutron and fusion-product diagnostics, and defect hydrodynamics.
ABSTRACT We present observations from 2007 March 2 of a partial filament eruption characterized b... more ABSTRACT We present observations from 2007 March 2 of a partial filament eruption characterized by two distinct phases of writhing motions: a quasi-static, slowly evolving phase followed by a rapid kinking phase showing a bifurcation of the filament. The quasi-static kinking motions ...
In a recent Letter, Reale & Peres demonstrated that this method can explain the almost isothe... more In a recent Letter, Reale & Peres demonstrated that this method can explain the almost isothermal appearance of T RACE loops (observed by Lenz et al.) as derived from the lter-ratio method. From model-tting of the 171 and 195 uxes of 41 loops, which have loop half-...
Publications of The Astronomical Society of The Pacific, 2007
We describe how the James Webb Space Telescope (JWST) Near-Infrared Spectrograph's (NIRSpec's) de... more We describe how the James Webb Space Telescope (JWST) Near-Infrared Spectrograph's (NIRSpec's) detectors will be read out, and present a model of how noise scales with the number of multiple non-destructive reads sampling-up-the-ramp. We believe that this noise model, which is validated using real and simulated test data, is applicable to most astronomical near-infrared instruments. We describe some non-ideal behaviors that have been observed in engineering grade NIRSpec detectors, and demonstrate that they are unlikely to affect NIRSpec sensitivity, operations, or calibration. These include a HAWAII-2RG reset anomaly and random telegraph noise (RTN). Using real test data, we show that the reset anomaly is: (1) very nearly noiseless and (2) can be easily calibrated out. Likewise, we show that large-amplitude RTN affects only a small and fixed population of pixels. It can therefore be tracked using standard pixel operability maps.
ABSTRACT We report here, for the rst time, on spatial oscillations of coronal loops, which were ... more ABSTRACT We report here, for the rst time, on spatial oscillations of coronal loops, which were detected in extreme-ultraviolet wavelengths (171 with the Transition Region and Coronal Explorer, in the tem-Aé) perature range of MK. The observed loop oscillations occurred ...
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