Purpose CHCHD2 is an anti-apoptotic mitochondrial protein acting through the BCL2/BAX pathway in ... more Purpose CHCHD2 is an anti-apoptotic mitochondrial protein acting through the BCL2/BAX pathway in various cancers. However, data on the regulatory role of CHCHD2 in adrenal tumourigenesis are scarce. Methods We studied the expression of CHCHD2, BCL2 and BAX in human adrenocortical tissues and SW-13 cells. mRNA and protein levels were analysed through qPCR and immunoblotting respectively in 16 benign adrenocortical neoplasms (BANs) and 10 adrenocortical carcinomas (ACCs) along with the adjacent normal adrenal tissues (controls). BCL2/BAX mRNA expression was also analyzed in SW-13 cells after CHCHD2 silencing. MTS, flow cytometry and scratch assays were performed to assess cell viability, apoptosis and invasion respectively. Results BCL2 and CHCHCD2 mRNA and protein expression was increased in BANs compared with normal adrenal tissues whereas BAX was decreased. BAX and CHCHD2 mRNA and protein levels were significantly down-regulated and up-regulated respectively in ACCs compared to eit...
1- MassArray data on different FOXD3 amplicons. Raw data used in FOXD3 methylation analysis prese... more 1- MassArray data on different FOXD3 amplicons. Raw data used in FOXD3 methylation analysis presented in figure 1C. 2- Protein quantification in Paraganglioma (PGL) and GIST samples. Measurements of band densitometry to analyze FOXD3 expression in PGL and GIST samples presented in figure 2A. 3- Luciferase assay to determine FOXD3 activation. Raw data of Luciferase assay performed to study protein activation in different FOXD3 variants. Data is presented in fig 3A. 4- Measurements of band densitometry to analyze FOXD3 and c-Kit protein expression in HEK and NTERA-2 cells after FOXD3 siRNA assay. Raw data used in the HEK293 and NTERA-2 Protein quantification analysis in the fig 3B. 5- Positive c-kit cells quantification after c-kit immunostaining in inner circular muscularis and outer longitudinal muscularis of interstitial cells of Cajal of wild type and FOXD3+/- mice. Data is presented in figure 5B. Raw data of muscle thickness measurement of the ileum of wild type and FOXD3+/- mice...
Diabetes insipidus (DI) is a well‐recognised transient or permanent complication following transs... more Diabetes insipidus (DI) is a well‐recognised transient or permanent complication following transsphenoidal surgery for pituitary adenomas or other sellar/parasellar lesions. However, data regarding the prevalence of pre‐operative DI in sellar/parasellar lesions other than pituitary adenomas are scarce. We systematically reviewed the existing data for defining the prevalence of DI before any treatment in adult patients with sellar/parasellar lesions, excluding pituitary adenomas and metastatic lesions. In total, 646 patients with sellar/parasellar lesions presenting with DI at diagnosis were identified. The most common pathologies of sellar/parasellar lesions presenting with DI at diagnosis were lymphocytic hypophysitis (26.5%), craniopharyngiomas (23.4%), Langerhans's cell histiocytosis (18.9%) and Rathke's cleft cyst (12.7%), accounting for the vast majority (more than 80%) of these lesions. Overall, DI at diagnosis was found in 23.4% of all patients with sellar/parasellar lesions, albeit with a wide range from 10.6% to 76.7%, depending on the nature of the pathology. The highest prevalence of DI was found in less commonly encountered lesions namely germ‐cell tumours (76.7%), abscesses (55.4%) and neurosarcoidosis (54.5%), each accounting for less than 3% of all sellar/parasellar lesions. Most DI cases (68.8%) were associated with anterior pituitary hormonal deficiencies, in contrast to pituitary adenomas that rarely present with DI. The enlargement and enhancement of the pituitary stalk were the most common findings on magnetic resonance imaging besides the loss of the high signal of the posterior pituitary on T1‐weighted images. Resolution of DI spontaneously or following systemic and surgical management occurred in 22.4% of cases. Post‐operative DI, not evident before surgery, was found in 27.8% of non‐adenomatous sellar/parasellar lesions, and was transient in 11.6% of them. Besides distinctive imaging features and symptoms, early recognition of DI in such lesions is important because it directs the diagnosis towards a non‐adenomatous sellar/parasellar tumour and the early initiation of appropriate treatment.
Purpose CHCHD2 is an anti-apoptotic mitochondrial protein acting through the BCL2/BAX pathway in ... more Purpose CHCHD2 is an anti-apoptotic mitochondrial protein acting through the BCL2/BAX pathway in various cancers. However, data on the regulatory role of CHCHD2 in adrenal tumourigenesis are scarce. Methods We studied the expression of CHCHD2, BCL2 and BAX in human adrenocortical tissues and SW-13 cells. mRNA and protein levels were analysed through qPCR and immunoblotting respectively in 16 benign adrenocortical neoplasms (BANs) and 10 adrenocortical carcinomas (ACCs) along with the adjacent normal adrenal tissues (controls). BCL2/BAX mRNA expression was also analyzed in SW-13 cells after CHCHD2 silencing. MTS, flow cytometry and scratch assays were performed to assess cell viability, apoptosis and invasion respectively. Results BCL2 and CHCHCD2 mRNA and protein expression was increased in BANs compared with normal adrenal tissues whereas BAX was decreased. BAX and CHCHD2 mRNA and protein levels were significantly down-regulated and up-regulated respectively in ACCs compared to eit...
1- MassArray data on different FOXD3 amplicons. Raw data used in FOXD3 methylation analysis prese... more 1- MassArray data on different FOXD3 amplicons. Raw data used in FOXD3 methylation analysis presented in figure 1C. 2- Protein quantification in Paraganglioma (PGL) and GIST samples. Measurements of band densitometry to analyze FOXD3 expression in PGL and GIST samples presented in figure 2A. 3- Luciferase assay to determine FOXD3 activation. Raw data of Luciferase assay performed to study protein activation in different FOXD3 variants. Data is presented in fig 3A. 4- Measurements of band densitometry to analyze FOXD3 and c-Kit protein expression in HEK and NTERA-2 cells after FOXD3 siRNA assay. Raw data used in the HEK293 and NTERA-2 Protein quantification analysis in the fig 3B. 5- Positive c-kit cells quantification after c-kit immunostaining in inner circular muscularis and outer longitudinal muscularis of interstitial cells of Cajal of wild type and FOXD3+/- mice. Data is presented in figure 5B. Raw data of muscle thickness measurement of the ileum of wild type and FOXD3+/- mice...
Diabetes insipidus (DI) is a well‐recognised transient or permanent complication following transs... more Diabetes insipidus (DI) is a well‐recognised transient or permanent complication following transsphenoidal surgery for pituitary adenomas or other sellar/parasellar lesions. However, data regarding the prevalence of pre‐operative DI in sellar/parasellar lesions other than pituitary adenomas are scarce. We systematically reviewed the existing data for defining the prevalence of DI before any treatment in adult patients with sellar/parasellar lesions, excluding pituitary adenomas and metastatic lesions. In total, 646 patients with sellar/parasellar lesions presenting with DI at diagnosis were identified. The most common pathologies of sellar/parasellar lesions presenting with DI at diagnosis were lymphocytic hypophysitis (26.5%), craniopharyngiomas (23.4%), Langerhans's cell histiocytosis (18.9%) and Rathke's cleft cyst (12.7%), accounting for the vast majority (more than 80%) of these lesions. Overall, DI at diagnosis was found in 23.4% of all patients with sellar/parasellar lesions, albeit with a wide range from 10.6% to 76.7%, depending on the nature of the pathology. The highest prevalence of DI was found in less commonly encountered lesions namely germ‐cell tumours (76.7%), abscesses (55.4%) and neurosarcoidosis (54.5%), each accounting for less than 3% of all sellar/parasellar lesions. Most DI cases (68.8%) were associated with anterior pituitary hormonal deficiencies, in contrast to pituitary adenomas that rarely present with DI. The enlargement and enhancement of the pituitary stalk were the most common findings on magnetic resonance imaging besides the loss of the high signal of the posterior pituitary on T1‐weighted images. Resolution of DI spontaneously or following systemic and surgical management occurred in 22.4% of cases. Post‐operative DI, not evident before surgery, was found in 27.8% of non‐adenomatous sellar/parasellar lesions, and was transient in 11.6% of them. Besides distinctive imaging features and symptoms, early recognition of DI in such lesions is important because it directs the diagnosis towards a non‐adenomatous sellar/parasellar tumour and the early initiation of appropriate treatment.
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Papers by Anna Angelousi