Journal of Shahid Sadoughi University of Medical Sciences
Introduction: The brachial plexus is the largest and most complex nerve network in the body, whic... more Introduction: The brachial plexus is the largest and most complex nerve network in the body, which is formed in the neck and enters the axillary cavity after passing under the clavicle. So far, many variations have been reported in the brachial network, which are clinically important. The plexus has three cords, one of which is the medial cord. The medial cutaneous nerve of the arm and the medial cutaneous nerve of the forearm are the branches of the medial cord that innervate the skin of the medial surface of the arm and forearm, respectively. In this study, a rare variation was observed in the branches of the medial cord of the brachial plexus during the dissection of the upper limb of an approximately 80-year-old man in the Dissection Hall of Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Thus, in normal condition, there is no connection between the medial cutaneous nerve of the arm and the medial cutaneous nerve of the forearm, and each of them innervates the skin o...
The present experimental study was undertaken to investigate the effect of formaldehyde (FA) and ... more The present experimental study was undertaken to investigate the effect of formaldehyde (FA) and curcumin (CUR) on histomorphological features, antioxidant potential, and messenger ribonucleic acid (mRNA) levels of genes related to follicular development in FA-exposed rats. 24 Wistar female rats were divided into four study groups and given intraperitoneal injections of FA (10 mg/kg) (N = 6), FA (10 mg/kg) + CUR (100 mg/kg) (N = 6), sham (N = 6), and control (N = 6) for 14 days. Ovarian follicular histology, the related gene expression, blood factors, and anti/oxidation potentials were assessed using ovarian tissue and serum, respectively. The klotho was significantly overexpressed in the FA group compared with controls and shams. Contradictory, the factor in germ line alpha was significantly down-regulated in FA and FA + CUR groups compared to shams and controls. A significant decline was seen in the number of ovarian follicles in the FA group, independent of the developmental stag...
Recently, it has been proven that cartilage healing is difficult. The most commonly used treatmen... more Recently, it has been proven that cartilage healing is difficult. The most commonly used treatments are autogenously cartilage grafting and allogeneic bone grafting, but grafts cannot fully meet treatment goals because of source, price, safety, and other concerns. Thus, a combination of biological materials and tissue engineering technology has become a recent trend in studies. Among the studies performed on tissue engineering cartilage materials are hydrogels that exhibit biological activity, post-decomposition adsorption, flexibility, and easy preparation. Cell-containing hydrogels are often used in cartilage tissue engineering because of their biocompatibility, ease of use, and ability to adapt to different defects. Hydrogels are used to mimic extracellular matrices. Although multiple materials can configure and form hydrogels, hyaluronic acid and its derivatives are distinguished. Hyaluronic acid (HA) is an extracellular molecule with several physical and biological functions fo...
Introduction: Many studies have shown that medicinal herbs despite several therapeutic effects, s... more Introduction: Many studies have shown that medicinal herbs despite several therapeutic effects, sometimes can have many side effects for the fetus and even lead to miscarriage. Celery (Apiumgraveolens) is a medicinal plant in traditional medicine with numerous therapeutic effects. However, there is no information regarding its effects on the fetus; therefore, this study was performed with aim to evaluate the effects of hydro alcoholic extract of celery leaves on Balb/c mice embryo. Methods: This interventional study was performed from June 20 to 10 July 2016 on 20 female mice which were buy from the animal center of Yazd Shahid Sadoughi University of Medical Sciences in histology laboratory of the Anatomy Department of Shahid Sadoughi University of Medical Sciences in Yazd. 20 female mice after mating were randomly divided into 4 groups. Control group received distilled water (gavage) and experimental groups 1، 2 and 3 were received 200mg/kg of the celery hydro alcoholic extract dur...
Poly(lactic-co-glycolic) acid (PLGA) has attracted a considerable amount of interest for biomedic... more Poly(lactic-co-glycolic) acid (PLGA) has attracted a considerable amount of interest for biomedical application due to its biocompatibility, tailored biodegradation rate (depending on the molecular weight and copolymer ratio), approval for clinical use in humans by the U.S. Food and Drug Administration (FDA), the potential to change surface properties to create better interaction with biological materials and being suitable for export to countries and cultures where planting products with animals is unusable. For commercial use and research, PLGA has been widely studied to control small molecule drugs, proteins, and other macromolecules. This study aims to review the studies that used PLGA scaffolding and its composites as a scaffold and drug delivery in cartilage tissue engineering. It is concluded from the results that the PLGA scaffold as a synthetic scaffold, when combined with natural scaffolds or hybrids, strengthens its biological properties and performs its function better.
Background: Tamoxifen (TX) is widely used to treat idiopathic infertility in men. Using TX has be... more Background: Tamoxifen (TX) is widely used to treat idiopathic infertility in men. Using TX has been shown to produce sperm in patients with oligospermia and azoospermia and improve male fertility. The aim of this study was to evaluate the effects of TX on DNA and chromatin quality in mice regarding the importance of chromatin quality and sperm DNA at all stages of reproduction. Methods: 24 male NMRI mice were divided into 3 groups including dose 0.4 mg/kg/day that received basal diet and TX, dose 0.6 mg/kg/day that received basal diet and TX, and group 3 that received vehicle for 35 days as the control. After that, epididymal spermatozoa were analyzed for nuclear DNA quality. One-way ANOVA was performed with a Tukey test to compare sperm DNA fragmentation at different times. The p<0.05 was considered significant. Results: The use of different doses of TX may have detrimental effects on sperm chromatin protamination and DNA integrity in mice. According to Acridine Orange (AO) stai...
Objectives: The present study aimed to evaluate the sperm chromatin quality and rate of apoptosis... more Objectives: The present study aimed to evaluate the sperm chromatin quality and rate of apoptosis in formaldehyde (FA)-exposed mice and the possible protective effects of curcumin (Cur) on the fertility potential of spermatozoa. Methods: Twenty-four adult male NMRI mice were randomly divided into three groups: Group Ι (control), group ΙΙ (Sham) received Cur solvent (2 ml/day, intraperitoneal), group ΙΙΙ received FA (10 mg/kg, intraperitoneal), and group ΙV received FA+Cur (100 mg/kg, intraperitoneal). After 35 days, the spermatozoa from right cauda epididymis were analyzed using Chromomycin A3 (CMA3) and TUNEL staining. Result: Regarding CMA3 and TUNEL tests, the data revealed a significant increase (p <0.05) in control and sham groups compared to the FA and FA+Cur groups. There was also a significant decrease in CMA3 tests and apoptosis in FA+Cur group compared to FA group (p <0.05). Conclusion: Cur, as a potent antioxidant, can attenuate detrimental effects of FA on the chro...
1 Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, ... more 1 Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 2 Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 3 Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 4 Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Introduction: Considering the development of nanotechnology and extensive use of nano-materials a... more Introduction: Considering the development of nanotechnology and extensive use of nano-materials are in different fields of industry, it is necessary to investigate their destructive effects on biological systems. Titanium dioxide (TiO2) is used in the production of different dyes, cosmetics, ceramics, photocatalysts, water and sewage treatment and a lot of other products. This study was performed with aim to evaluate the Teratogenic effect of TiO2 in different gestational weeks of NMRI mice. Methods: This experimental study was performed on 30 female NMRI mice (25-30 gr, 8-12 weeks of pregnancy) in 2017. The mice were randomly assigned into 2 control groups and 4 case groups). Pregnancy was confirmed with vaginal plaque (GD0). The doses of 5 mg/kg of tio2 solution were orally gavage to pregnant mice from the first to 18th days of pregnancy. The first group, the first week of pregnancy, the second group, the second week of pregnancy and the third group received nanoparticles only in ...
The use of ovarian stimulation for infertility treatment is associated with side effects of ovari... more The use of ovarian stimulation for infertility treatment is associated with side effects of ovarian hyperstimulation syndrome (OHSS) and potential cancer risk. This is also true in high risk women such as those polycystic with ovary (PCO) and polycystic ovarian syndrome (PCOS). In vitro maturation (IVM) of oocytes was primarily developed to make IVF safe for women with PCO and at high risk of OHSS. The application of IVM of oocytes to assist clinical infertility treatment remains poor because of the reduced developmental competence of oocytes after IVM, despite several decades of research. Reduced meiotic maturation and fertilization rates, as well as low blastocyst production reveal short-term developmental insufficiency of oocytes when compared with in vivo-matured counterparts. In this review, the structural role of human oocytes, revealed by different technical approaches, to the success of IVM technology is highlighted.
Journal of Shahid Sadoughi University of Medical Sciences
The brachial plexus is the largest and most complex neural plexus in the body located in the neck... more The brachial plexus is the largest and most complex neural plexus in the body located in the neck and axilla. So far, there have been many reports of various variations in the brachial plexus that are of clinical significance. In the present study, while dissecting the upper limb of a 54-year-old man in the dissection room of Shahid Sadoughi University of Medical Sciences in Yazd, a rare variation was observed in the lateral cord branches of the plexus. In this case, the medial pectoral nerve, which normally separates from the medial cord, originated from the lateral cord. Awareness of this variation is important for anatomists, surgeons, anesthesiologists and radiologists to interpret the graphs, etc., and can help to reduce clinical complications during surgery and better interpret and diagnose the graphs.
Background and Aims: Formaldehyde (FA) exposure is an important cause of cellular injury and oxid... more Background and Aims: Formaldehyde (FA) exposure is an important cause of cellular injury and oxidative damage in testis, leading to infertility. This study aimed to assess the protective effects of curcumin on sperm and stereological parameters in testes from formaldehyde-exposed NMRI mice. Materials and Methods: At 6-8 weeks of age, 24 adult male NMRI mice weighing 30-35 g were categorized into four groups (n=6) based on the treatment they received: Group І (control) received no treatment, group ΙΙ received FA (10 mg/kg), group ΙΙΙ received FA (10 mg/kg) and curcumin (100 mg/kg), and group IV (Solvent) received dimethyl sulfoxide (0.2 ml/day). Materials were administered intraperitoneally for 35 days. After excision, epididymis tissues were placed in 1 mL aliquots of Ham’s F10 medium at 37˚C for 20 min and were then used in analyses of sperm parameters. Testes were fixed and stained with Hematoxylin & Eosin to investigate stereological indices. We also determined lipid peroxidation...
Objective: The chief outcome of testicular torsion in clinical and experimental contexts is testi... more Objective: The chief outcome of testicular torsion in clinical and experimental contexts is testicular ischemia. Curcumin, a compound with anti-inflammatory and antioxidant properties, has fascinated researchers and clinicians for its promise in the treatment of fertility diseases. Methods: Thirty-five fully grown male mice were randomly classified into five groups: control, sham, testicular torsion, treatment group 1 (testicular torsion+short-term curcumin), and treatment group 2 (testicular torsion+long-term curcumin). Thirty-five days later, spermatozoa from the right cauda epididymis were analyzed with regard to count and motility. Toluidine blue (TB), aniline blue (AB), and chromomycin A3 (CMA3) staining assays were used to evaluate the sperm chromatin integrity. In addition, the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) test was used to assess apoptosis.Result: Treatment group 1 exhibited a remarkably elevated sperm coun...
Background and Aims: This study aimed to observe the effect of Icariin on histomorphometric chang... more Background and Aims: This study aimed to observe the effect of Icariin on histomorphometric changes of testis and prostate induced by Acrylamide. Materials and Methods: Male mice were divided into four groups (n=8): A is the control group and does not get any treatment, B is the sham group and only received drinking water. C group received Acrylamide 10 mg/kg. D group received Acrylamide 15 mg/kg+1.5 mg/kg of Icariin. Histological changes in testis and prostate were examined using stereological methods. Results: Results showed decreases in testis weight of the group treated by (p≤0.01) and the group cured by Acrylamide +Icariin group (p≤0.05). The total volume of testis showed a reduction in the Acrylamide group compared to other groups (p≤0.05). The total number of spermatogonia and spermatocyte cells in the Acrylamide group showed a decrease in comparison with the other groups (p≤0.05). The total number of spermatid cells in the Acrylamide group indicated a significant reduction ...
Objective: The present study investigated sperm chromatin quality and testosterone levels in acry... more Objective: The present study investigated sperm chromatin quality and testosterone levels in acrylamide-treated mice and the possible protective effects of vitamin E on the fertility potential of spermatozoa. Methods: Thirty-two adult male mice were divided equally into four groups. Group 1 was the control, group 2 received acrylamide (10 mg/kg, water solution), group 3 received vitamin E (100 mg/kg, intraperitoneal), and group 4 received both acrylamide and vitamin E. After 35 days, spermatozoa from the right cauda epididymis were analyzed in terms of count, motility, morphology, and viability. Sperm DNA integrity and chromatin condensation were assessed by acridine orange (AO), aniline blue (AB), toluidine blue (TB), and chromomycin A3 (CMA3) staining. Results: In acrylamide-treated mice, significantly lower sperm concentration, viability, motility, and testosterone levels were found in comparison with the control and acrylamide+vitamin E groups (p<0.05). In the vitamin E group...
Journal of Shahid Sadoughi University of Medical Sciences
Introdcution: The axillary artery extends to the subclavian artery, which gives blood the axillar... more Introdcution: The axillary artery extends to the subclavian artery, which gives blood the axillary region. This artery is starting from the outset of the first ribAnd ends near the inferior terse major muscle, and then goes on with the name of the Brachial artery,the axillary artery variation is common and given the blood supply to the upper limb Clinically important .These variations seem to be an important finding that a rare case of anatomical presentation in an underground cavity to the right of a male body with an age of approximately 60-65 years was found in ShahidSadoughi University of Medical Sciences in Yazd, which was as followed:, external thoracic artery that is normally separated from the second part of the axillary artery;In this case, the third branch of the Thoracodorsal artery is separated. Since the axillary artery was exposed to damage, especially in cases of abdominal tenderness or removal of the axillary lymph nodes, the use of upper limb arches for coronary art...
Journal of Shahid Sadoughi University of Medical Sciences
Introduction: The brachial plexus is the largest and most complex nerve network in the body, whic... more Introduction: The brachial plexus is the largest and most complex nerve network in the body, which is formed in the neck and enters the axillary cavity after passing under the clavicle. So far, many variations have been reported in the brachial network, which are clinically important. The plexus has three cords, one of which is the medial cord. The medial cutaneous nerve of the arm and the medial cutaneous nerve of the forearm are the branches of the medial cord that innervate the skin of the medial surface of the arm and forearm, respectively. In this study, a rare variation was observed in the branches of the medial cord of the brachial plexus during the dissection of the upper limb of an approximately 80-year-old man in the Dissection Hall of Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Thus, in normal condition, there is no connection between the medial cutaneous nerve of the arm and the medial cutaneous nerve of the forearm, and each of them innervates the skin o...
The present experimental study was undertaken to investigate the effect of formaldehyde (FA) and ... more The present experimental study was undertaken to investigate the effect of formaldehyde (FA) and curcumin (CUR) on histomorphological features, antioxidant potential, and messenger ribonucleic acid (mRNA) levels of genes related to follicular development in FA-exposed rats. 24 Wistar female rats were divided into four study groups and given intraperitoneal injections of FA (10 mg/kg) (N = 6), FA (10 mg/kg) + CUR (100 mg/kg) (N = 6), sham (N = 6), and control (N = 6) for 14 days. Ovarian follicular histology, the related gene expression, blood factors, and anti/oxidation potentials were assessed using ovarian tissue and serum, respectively. The klotho was significantly overexpressed in the FA group compared with controls and shams. Contradictory, the factor in germ line alpha was significantly down-regulated in FA and FA + CUR groups compared to shams and controls. A significant decline was seen in the number of ovarian follicles in the FA group, independent of the developmental stag...
Recently, it has been proven that cartilage healing is difficult. The most commonly used treatmen... more Recently, it has been proven that cartilage healing is difficult. The most commonly used treatments are autogenously cartilage grafting and allogeneic bone grafting, but grafts cannot fully meet treatment goals because of source, price, safety, and other concerns. Thus, a combination of biological materials and tissue engineering technology has become a recent trend in studies. Among the studies performed on tissue engineering cartilage materials are hydrogels that exhibit biological activity, post-decomposition adsorption, flexibility, and easy preparation. Cell-containing hydrogels are often used in cartilage tissue engineering because of their biocompatibility, ease of use, and ability to adapt to different defects. Hydrogels are used to mimic extracellular matrices. Although multiple materials can configure and form hydrogels, hyaluronic acid and its derivatives are distinguished. Hyaluronic acid (HA) is an extracellular molecule with several physical and biological functions fo...
Introduction: Many studies have shown that medicinal herbs despite several therapeutic effects, s... more Introduction: Many studies have shown that medicinal herbs despite several therapeutic effects, sometimes can have many side effects for the fetus and even lead to miscarriage. Celery (Apiumgraveolens) is a medicinal plant in traditional medicine with numerous therapeutic effects. However, there is no information regarding its effects on the fetus; therefore, this study was performed with aim to evaluate the effects of hydro alcoholic extract of celery leaves on Balb/c mice embryo. Methods: This interventional study was performed from June 20 to 10 July 2016 on 20 female mice which were buy from the animal center of Yazd Shahid Sadoughi University of Medical Sciences in histology laboratory of the Anatomy Department of Shahid Sadoughi University of Medical Sciences in Yazd. 20 female mice after mating were randomly divided into 4 groups. Control group received distilled water (gavage) and experimental groups 1، 2 and 3 were received 200mg/kg of the celery hydro alcoholic extract dur...
Poly(lactic-co-glycolic) acid (PLGA) has attracted a considerable amount of interest for biomedic... more Poly(lactic-co-glycolic) acid (PLGA) has attracted a considerable amount of interest for biomedical application due to its biocompatibility, tailored biodegradation rate (depending on the molecular weight and copolymer ratio), approval for clinical use in humans by the U.S. Food and Drug Administration (FDA), the potential to change surface properties to create better interaction with biological materials and being suitable for export to countries and cultures where planting products with animals is unusable. For commercial use and research, PLGA has been widely studied to control small molecule drugs, proteins, and other macromolecules. This study aims to review the studies that used PLGA scaffolding and its composites as a scaffold and drug delivery in cartilage tissue engineering. It is concluded from the results that the PLGA scaffold as a synthetic scaffold, when combined with natural scaffolds or hybrids, strengthens its biological properties and performs its function better.
Background: Tamoxifen (TX) is widely used to treat idiopathic infertility in men. Using TX has be... more Background: Tamoxifen (TX) is widely used to treat idiopathic infertility in men. Using TX has been shown to produce sperm in patients with oligospermia and azoospermia and improve male fertility. The aim of this study was to evaluate the effects of TX on DNA and chromatin quality in mice regarding the importance of chromatin quality and sperm DNA at all stages of reproduction. Methods: 24 male NMRI mice were divided into 3 groups including dose 0.4 mg/kg/day that received basal diet and TX, dose 0.6 mg/kg/day that received basal diet and TX, and group 3 that received vehicle for 35 days as the control. After that, epididymal spermatozoa were analyzed for nuclear DNA quality. One-way ANOVA was performed with a Tukey test to compare sperm DNA fragmentation at different times. The p<0.05 was considered significant. Results: The use of different doses of TX may have detrimental effects on sperm chromatin protamination and DNA integrity in mice. According to Acridine Orange (AO) stai...
Objectives: The present study aimed to evaluate the sperm chromatin quality and rate of apoptosis... more Objectives: The present study aimed to evaluate the sperm chromatin quality and rate of apoptosis in formaldehyde (FA)-exposed mice and the possible protective effects of curcumin (Cur) on the fertility potential of spermatozoa. Methods: Twenty-four adult male NMRI mice were randomly divided into three groups: Group Ι (control), group ΙΙ (Sham) received Cur solvent (2 ml/day, intraperitoneal), group ΙΙΙ received FA (10 mg/kg, intraperitoneal), and group ΙV received FA+Cur (100 mg/kg, intraperitoneal). After 35 days, the spermatozoa from right cauda epididymis were analyzed using Chromomycin A3 (CMA3) and TUNEL staining. Result: Regarding CMA3 and TUNEL tests, the data revealed a significant increase (p <0.05) in control and sham groups compared to the FA and FA+Cur groups. There was also a significant decrease in CMA3 tests and apoptosis in FA+Cur group compared to FA group (p <0.05). Conclusion: Cur, as a potent antioxidant, can attenuate detrimental effects of FA on the chro...
1 Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, ... more 1 Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 2 Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. 3 Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. 4 Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Introduction: Considering the development of nanotechnology and extensive use of nano-materials a... more Introduction: Considering the development of nanotechnology and extensive use of nano-materials are in different fields of industry, it is necessary to investigate their destructive effects on biological systems. Titanium dioxide (TiO2) is used in the production of different dyes, cosmetics, ceramics, photocatalysts, water and sewage treatment and a lot of other products. This study was performed with aim to evaluate the Teratogenic effect of TiO2 in different gestational weeks of NMRI mice. Methods: This experimental study was performed on 30 female NMRI mice (25-30 gr, 8-12 weeks of pregnancy) in 2017. The mice were randomly assigned into 2 control groups and 4 case groups). Pregnancy was confirmed with vaginal plaque (GD0). The doses of 5 mg/kg of tio2 solution were orally gavage to pregnant mice from the first to 18th days of pregnancy. The first group, the first week of pregnancy, the second group, the second week of pregnancy and the third group received nanoparticles only in ...
The use of ovarian stimulation for infertility treatment is associated with side effects of ovari... more The use of ovarian stimulation for infertility treatment is associated with side effects of ovarian hyperstimulation syndrome (OHSS) and potential cancer risk. This is also true in high risk women such as those polycystic with ovary (PCO) and polycystic ovarian syndrome (PCOS). In vitro maturation (IVM) of oocytes was primarily developed to make IVF safe for women with PCO and at high risk of OHSS. The application of IVM of oocytes to assist clinical infertility treatment remains poor because of the reduced developmental competence of oocytes after IVM, despite several decades of research. Reduced meiotic maturation and fertilization rates, as well as low blastocyst production reveal short-term developmental insufficiency of oocytes when compared with in vivo-matured counterparts. In this review, the structural role of human oocytes, revealed by different technical approaches, to the success of IVM technology is highlighted.
Journal of Shahid Sadoughi University of Medical Sciences
The brachial plexus is the largest and most complex neural plexus in the body located in the neck... more The brachial plexus is the largest and most complex neural plexus in the body located in the neck and axilla. So far, there have been many reports of various variations in the brachial plexus that are of clinical significance. In the present study, while dissecting the upper limb of a 54-year-old man in the dissection room of Shahid Sadoughi University of Medical Sciences in Yazd, a rare variation was observed in the lateral cord branches of the plexus. In this case, the medial pectoral nerve, which normally separates from the medial cord, originated from the lateral cord. Awareness of this variation is important for anatomists, surgeons, anesthesiologists and radiologists to interpret the graphs, etc., and can help to reduce clinical complications during surgery and better interpret and diagnose the graphs.
Background and Aims: Formaldehyde (FA) exposure is an important cause of cellular injury and oxid... more Background and Aims: Formaldehyde (FA) exposure is an important cause of cellular injury and oxidative damage in testis, leading to infertility. This study aimed to assess the protective effects of curcumin on sperm and stereological parameters in testes from formaldehyde-exposed NMRI mice. Materials and Methods: At 6-8 weeks of age, 24 adult male NMRI mice weighing 30-35 g were categorized into four groups (n=6) based on the treatment they received: Group І (control) received no treatment, group ΙΙ received FA (10 mg/kg), group ΙΙΙ received FA (10 mg/kg) and curcumin (100 mg/kg), and group IV (Solvent) received dimethyl sulfoxide (0.2 ml/day). Materials were administered intraperitoneally for 35 days. After excision, epididymis tissues were placed in 1 mL aliquots of Ham’s F10 medium at 37˚C for 20 min and were then used in analyses of sperm parameters. Testes were fixed and stained with Hematoxylin & Eosin to investigate stereological indices. We also determined lipid peroxidation...
Objective: The chief outcome of testicular torsion in clinical and experimental contexts is testi... more Objective: The chief outcome of testicular torsion in clinical and experimental contexts is testicular ischemia. Curcumin, a compound with anti-inflammatory and antioxidant properties, has fascinated researchers and clinicians for its promise in the treatment of fertility diseases. Methods: Thirty-five fully grown male mice were randomly classified into five groups: control, sham, testicular torsion, treatment group 1 (testicular torsion+short-term curcumin), and treatment group 2 (testicular torsion+long-term curcumin). Thirty-five days later, spermatozoa from the right cauda epididymis were analyzed with regard to count and motility. Toluidine blue (TB), aniline blue (AB), and chromomycin A3 (CMA3) staining assays were used to evaluate the sperm chromatin integrity. In addition, the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) test was used to assess apoptosis.Result: Treatment group 1 exhibited a remarkably elevated sperm coun...
Background and Aims: This study aimed to observe the effect of Icariin on histomorphometric chang... more Background and Aims: This study aimed to observe the effect of Icariin on histomorphometric changes of testis and prostate induced by Acrylamide. Materials and Methods: Male mice were divided into four groups (n=8): A is the control group and does not get any treatment, B is the sham group and only received drinking water. C group received Acrylamide 10 mg/kg. D group received Acrylamide 15 mg/kg+1.5 mg/kg of Icariin. Histological changes in testis and prostate were examined using stereological methods. Results: Results showed decreases in testis weight of the group treated by (p≤0.01) and the group cured by Acrylamide +Icariin group (p≤0.05). The total volume of testis showed a reduction in the Acrylamide group compared to other groups (p≤0.05). The total number of spermatogonia and spermatocyte cells in the Acrylamide group showed a decrease in comparison with the other groups (p≤0.05). The total number of spermatid cells in the Acrylamide group indicated a significant reduction ...
Objective: The present study investigated sperm chromatin quality and testosterone levels in acry... more Objective: The present study investigated sperm chromatin quality and testosterone levels in acrylamide-treated mice and the possible protective effects of vitamin E on the fertility potential of spermatozoa. Methods: Thirty-two adult male mice were divided equally into four groups. Group 1 was the control, group 2 received acrylamide (10 mg/kg, water solution), group 3 received vitamin E (100 mg/kg, intraperitoneal), and group 4 received both acrylamide and vitamin E. After 35 days, spermatozoa from the right cauda epididymis were analyzed in terms of count, motility, morphology, and viability. Sperm DNA integrity and chromatin condensation were assessed by acridine orange (AO), aniline blue (AB), toluidine blue (TB), and chromomycin A3 (CMA3) staining. Results: In acrylamide-treated mice, significantly lower sperm concentration, viability, motility, and testosterone levels were found in comparison with the control and acrylamide+vitamin E groups (p<0.05). In the vitamin E group...
Journal of Shahid Sadoughi University of Medical Sciences
Introdcution: The axillary artery extends to the subclavian artery, which gives blood the axillar... more Introdcution: The axillary artery extends to the subclavian artery, which gives blood the axillary region. This artery is starting from the outset of the first ribAnd ends near the inferior terse major muscle, and then goes on with the name of the Brachial artery,the axillary artery variation is common and given the blood supply to the upper limb Clinically important .These variations seem to be an important finding that a rare case of anatomical presentation in an underground cavity to the right of a male body with an age of approximately 60-65 years was found in ShahidSadoughi University of Medical Sciences in Yazd, which was as followed:, external thoracic artery that is normally separated from the second part of the axillary artery;In this case, the third branch of the Thoracodorsal artery is separated. Since the axillary artery was exposed to damage, especially in cases of abdominal tenderness or removal of the axillary lymph nodes, the use of upper limb arches for coronary art...
Uploads
Papers