ABSTRACT The dried bark of Phellodendron amurense Rupr., known as “Guanhuangbo” in China, has bee... more ABSTRACT The dried bark of Phellodendron amurense Rupr., known as “Guanhuangbo” in China, has been widely used as a traditional Chinese medicine for thousands of years. In this paper, an accurate and reliable method using high performance liquid chromatography coupled with diode array detection and mass spectrometry (HPLC-DAD-MS) was developed for the quality evaluation of wild Guanhuangbo. Six bioactive compounds, namely, chlorogenic acid, phellodendrine, magnoflorine, jatrorrhizine, palmatine and berberine, were determined simultaneously in 37 batches of wild Guanhuangbo samples collected from different locations in China. The chromatographic conditions and extraction procedures were optimized by an orthogonal design during the study and the identities of the compounds were confirmed by LC-MS. Moreover, similarity analysis and hierarchical clustering analysis were successfully applied to demonstrate the variability of these wild Guanhuangbo samples and the data obtained from different analysis showed good consistency. The results indicated that the developed multi-compound determination method in combination with fingerprint analysis is suitable for the quantitative analysis and quality evaluation of the dried bark of wild Phellodendron amurense Rupr.
Longmen Shan tectonic belt located in West Sichuan Plateau, which keeps the good forests outlook ... more Longmen Shan tectonic belt located in West Sichuan Plateau, which keeps the good forests outlook and provides a unique natural environment for the growth of medicinal plants because of its unique geological features. It is a area that is one of the most abundant biological diversity in China and the whole world range, and also is the one of the most concentrated area of the origin of Chinese herbal medicines in Sichuan Province and China. Wenchuan Earthquake induced thousands of landslides, debris flows, which made a huge impact on the medicinal plant habitat by means of severe fragmentation. In this paper, through evaluating and integrating the multi-period remote sensing image data and basic geographic information data of the Longmen Shan region after earthquake by grid technology, the image group that covers the whole Longmen Shan belt and reflects the different features of ground object is acquired. Characters of Wenchuan earthquake disaster distributing is interpreted and analy...
Two new spirobenzofuranoid dibenzocyclooctadiene lignans, kadsulignans O (1) and P (2) were isola... more Two new spirobenzofuranoid dibenzocyclooctadiene lignans, kadsulignans O (1) and P (2) were isolated from the stems of Kadsura oblongifolia, together with five known same type lignans, kadsulignans C (3), E (4), F (5), G (6), and heteroclitin J (7). Their structures were elucidated on the basis of spectral evidences and comparisons with literatures. Compounds 3-7 were isolated from this plant for the first time.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
To provide practical method for microscopic authentication of traditional Chinese medicine Gusuib... more To provide practical method for microscopic authentication of traditional Chinese medicine Gusuibu and its adulterants. By means of light microscope, scanning electron microscopy and tissue section techniques, the morphology, the size of the rhizome scales and their bearing position in the original plants of Gusuibu and its adulterants, i. e. Drynaria roosii, D. delavayi, D. quercifolia and Pseudodrynaria coronans were analyzed. There were significant differences between scales length of D. roosii, D. delavayi and P. coronans, while there was no significant difference between that of D. roosii and D. quercifolia. The scale teeth of D. delavayi were usually curved, bifid and uneven distributed at the scale fringe, which was different from that of the other three species. The base of the scales sinks in epidermis in D. roosii, D. quercifolia, and P. coronans, while it bore at the raised part of epidermis in D. delavayi. CONCLUTION: Morphology, size and bearing position of the rhizome ...
A rapid, sensitive, and accurate reversed-phase high-performance liquid chromatography with photo... more A rapid, sensitive, and accurate reversed-phase high-performance liquid chromatography with photodiode array detection method was developed for both quantitative determination of four compounds (caffeic acid-4-O-β-D-glucopyranoside, 5,7-dihydroxychromone-7-O-rutinoside, neoeriocitrin and naringin) and fingerprint analysis of the rhizomes of Drynaria fortunei (Kunze) J. Sm. The chromatographic separation was accomplished on an MZ-C18 column (4.6 × 250 mm, 5 μm) using gradient elution with acetonitrile and 0.02% aqueous acetic acid, at a flow rate of 1.0 mL min(-1), an operating temperature of 25°C, and a wavelength of 260 nm. The four compounds showed good regression relationship (R (2) > 0.9990) within linear ranges, and their recoveries were in the range of 98.11-102.23%. In the chromatographic fingerprint, thirteen common peaks were found and selected as characteristic peaks to assess the consistency of ten batches of the rhizomes of D. fortunei. The results indicate that the m...
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
To study the procedure of seed quality testing and seed grading scale of Phellodendron amurense. ... more To study the procedure of seed quality testing and seed grading scale of Phellodendron amurense. Seed quality testing methods were developed, which included the test of sampling, seed purity, weight per 1 000 seeds, seed moisture, seed viability and germination rate. The related data from 62 cases of seed specimens of P. amurense were analyzed by cluster analysis. The seed quality test procedure was developed, and the seed quality grading scale was formulated.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
Through the study of allelopathy of the pericarp of Phellodendron amurense, the role of self-rege... more Through the study of allelopathy of the pericarp of Phellodendron amurense, the role of self-regeneration barriers was investigated in order to find ways and means for the protection of wild populations of P. amurense. Solution preparation: soaked pericarp of P. amurense in distilled water at 4 degrees C to get solution A, and reflux extraction of pericarp with distilled water at 100 degrees C to get solution B. Both of the solution A and solution B were used in the experiment of seed germination and seedling growth with the seeds of cabbage and wheat. The results showed that 20 g x L(-1) concentration of solution A and solution B inhibited significantly seed germination of cabbage and wheat, while 100 g x L(-1) concentration of solution A even completely inhibited the seed germination of wheat. 20 g x L(-1) concentration of solution A significantly inhibited the cabbage and wheat seedling growth, completely inhibited the root growth of cabbage, while 100 g x L(-1) concentrations of...
In this paper, we briefly review international Good Agricultural Practice (GAP) regulations relat... more In this paper, we briefly review international Good Agricultural Practice (GAP) regulations related to traditional Chinese medicine herbs (TCM herbs) and the background of the drafting process and the implementation of GAP for TCM herbs in China. We also have summarized progress and achievements since the implementation of GAP for TCM herbs in 2002. Up to 2010, a total of 99 GAP bases were formally adopted by GAP certification. They cover 22 provinces/municipalities, and 49 species of TCM herbs are currently cultivated in these GAP bases. Finally, we discuss the main problems in GAP implementation for Chinese crude drugs and put forward some suggestions to tackle them.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
To investigate and arrange the ethno-pharmacological information of Schisandraceae plants in Chin... more To investigate and arrange the ethno-pharmacological information of Schisandraceae plants in China. The information was obtained by literature search, specimen inspection, field resource investigation and samples collection. Twenty-six Schisandraece plants (inlcuding 4 varieties) have been used as folk medicines in different regions of China, the identical medicinal parts of different species in one genus show the similar usage, action and indications, and different medicinal parts with different administrated ways show different action and indications. The results provide reliable information for resource development and comprehensive utilization of Schisandraece plant resource in China.
A rapid, sensitive, and accurate reversed-phase high-performance liquid chromatography with photo... more A rapid, sensitive, and accurate reversed-phase high-performance liquid chromatography with photodiode array detection method was developed for both quantitative determination of four compounds (caffeic acid-4-O-β-D-glucopyranoside, 5,7-dihydroxychromone-7-O-rutinoside, neoeriocitrin and naringin) and fingerprint analysis of the rhizomes of Drynaria fortunei (Kunze) J. Sm. The chromatographic separation was accomplished on an MZ-C18 column (4.6 × 250 mm, 5 μm) using gradient elution with acetonitrile and 0.02% aqueous acetic acid, at a flow rate of 1.0 mL min(-1), an operating temperature of 25°C, and a wavelength of 260 nm. The four compounds showed good regression relationship (R (2) > 0.9990) within linear ranges, and their recoveries were in the range of 98.11-102.23%. In the chromatographic fingerprint, thirteen common peaks were found and selected as characteristic peaks to assess the consistency of ten batches of the rhizomes of D. fortunei. The results indicate that the m...
Phytomedicine : international journal of phytotherapy and phytopharmacology
In China, the plants of genus Kadsura had been used as the folk medicines for a long time and sho... more In China, the plants of genus Kadsura had been used as the folk medicines for a long time and showed good effect of activating blood and dissolving stasis, promoting qi circulation to relieve pain, dispelling wind and eliminating dampness. The bioactivities of genus Kadsura were attributed to the existence of its characteristic chemical constituents. This review systematically summarized the traditional efficacy and medicinal application of genus Kadsura in China, chemical constituents and bioactivities of the plants of genus Kadsura. And, lignans and triterpenoids were the main bioactive constituents, which exhibited good anti-HIV, anti-tumor, anti-hepatitis, anti-oxidant, anti-platelet aggregation activities and neuroprotective effect etc. Moreover, some structure-activity relationships mining would greatly enrich the opportunity of finding new and promising lead compounds and promote the reasonable development and utilization of the plants of genus Kadsura.
Phytomedicine : international journal of phytotherapy and phytopharmacology, Jan 15, 2014
The stamen of lotus, known as Nelumbinis stamen, has been used as the folk medicine and functiona... more The stamen of lotus, known as Nelumbinis stamen, has been used as the folk medicine and functional food for a long time, which showed good activities of anti-ulcer, anti-thrombosis, analgesic, anti-diarrhea, strengthen uterine contraction. The bioactivities of Nelumbinis stamen were attributed to the existence of flavonoids, its characteristic chemical constituents. A reliable method for comprehensive chemical analysis of flavonoids in Nelumbinis stamen by HPLC-DAD-MS was developed for the first time. The extraction protocol of flavonoids from Nelumbinis stamen was optimized by an orthogonal design. The chromatographic conditions were optimized, which exhibited similar level than that of the UHPLC platform allowing target compound identification in a shorter time with little solvent consumption. Moreover, similarity analysis, hierarchical clustering analysis and principal components analysis were successfully applied to demonstrate the variability of these Nelumbinis stamen samples.
2010 18th International Conference on Geoinformatics, 2010
Longmen Shan tectonic belt located in West Sichuan Plateau, which keeps the good forests outlook ... more Longmen Shan tectonic belt located in West Sichuan Plateau, which keeps the good forests outlook and provides a unique natural environment for the growth of medicinal plants because of its unique geological features. It is a area that is one of the most abundant biological diversity in China and the whole world range, and also is the one of the most concentrated area of the origin of Chinese herbal medicines in Sichuan Province and China. Wenchuan Earthquake induced thousands of landslides, debris flows, which made a huge impact on the medicinal plant habitat by means of severe fragmentation. In this paper, through evaluating and integrating the multi-period remote sensing image data and basic geographic information data of the Longmen Shan region after earthquake by grid technology, the image group that covers the whole Longmen Shan belt and reflects the different features of ground object is acquired. Characters of Wenchuan earthquake disaster distributing is interpreted and analyzed by geographic information system platform for spatial analysis with the image group. Combine with the life habits of medicinal plants in this area and the main ecological factors impacting the spatial distribution pattern of medicinal plants, the impact on distribution pattern of medicinal plants habits during the disaster discussed, as a result, medicinal plants habitat influenced in the shock in various environmental factors is concluded. It can provide the academic evidence for medicinal plant restoration in earthquake zone.
In this paper, we briefly review international Good Agricultural Practice (GAP) regulations relat... more In this paper, we briefly review international Good Agricultural Practice (GAP) regulations related to traditional Chinese medicine herbs (TCM herbs) and the background of the drafting process and the implementation of GAP for TCM herbs in China. We also have summarized progress and achievements since the implementation of GAP for TCM herbs in 2002. Up to 2010, a total of 99 GAP bases were formally adopted by GAP certification. They cover 22 provinces/municipalities, and 49 species of TCM herbs are currently cultivated in these GAP bases. Finally, we discuss the main problems in GAP implementation for Chinese crude drugs and put forward some suggestions to tackle them.
The fruit of S. sphenanthera, known as &a... more The fruit of S. sphenanthera, known as "Nanwuweizi", has been widely used as traditional Chinese medicine for several thousand years. However, the current determination methods are not sufficient to evaluate its quality. An accurate, sensitive and reliable high performance liquid chromatography coupled with photodiode array detection and mass spectrum (HPLC-PAD-MS) was developed for quantitative analysis of twelve lignans (schisandrol A, schisandrol B, gomisin G, schisantherin A, schisantherin D, schisanhenol, (+)-anwulignan, deoxyschisandrin, schisandrin B, schisandrin C, 6-O-benzoylgomisin O, and interiotherin A) in the fruit of S. sphenanthera. The chromatographic conditions and extraction procedures were optimized during the study. The identity of chromatographic peaks in the sample HPLC profiles was confirmed by comparing the retention time, ultraviolet (UV) spectra and MS data with reference compounds. The validated method was successfully used to determine the twelve lignans in the samples collected from different localities in China. The hierarchical clustering analysis (HCA) and principal components analysis (PCA) were successfully applied to the data of twelve lignans from the HPLC profiles in sixteen batches of the fruit of S. sphenanthera to discriminate the samples with different sources. Moreover, the results of the loading plot of the PCA indicated that schisantherin A, (+)-anwulignan, and deoxyshisandrin were found to be the main constituents in the fruit of S. sphenanthera, and which could be chosen as the chemical markers for evaluate the quality of the fruit of S. sphenanthera. The results indicated that the developed method was readily utilized as a quality evaluation method for the fruit of S. sphenanthera.
International Conference on Infrared and Millimeter Waves, 2006
According to the concept of phase matching bandwidth the band width of broadband source by using ... more According to the concept of phase matching bandwidth the band width of broadband source by using retracing behavior of collinear quasi-phase-matching (CQPM) optical parametric generator (OPG) in periodically poled lithium niobate (PPLN) is presented firstly. Comparing the various pump wavelengths we obtain the pumping condition and parameters of PPLN to realize the broadband source near 1550 nm in the CQPM-OPG.
International Conference on Infrared and Millimeter Waves, 2006
Dual-signal-wave optical parametric oscillators (DSW OPOs) around 1.5 mum by using two periodical... more Dual-signal-wave optical parametric oscillators (DSW OPOs) around 1.5 mum by using two periodically poled crystals was demonstrated experimentally. The widely tunable wavelength interval from 2.5 nm to 69.1 nm was obtained. It can be used for generation of terahertz (THz) waves from 0.3-8.9 THz by different frequency generation. With the incident pump power of 3 W at 50 kHz, the
The theory of THz-wave generation (3.29 THz) via difference frequency generation (DFG) of near in... more The theory of THz-wave generation (3.29 THz) via difference frequency generation (DFG) of near infrared radiations (1318.8 nm and 1338.2 nm) by a simultaneous dual-wavelength lasing (SDWL) of an Nd: YAG laser in slant-stripe-type MgO-doped periodically poled lithium niobate (MgO: PPLN) was presented. It is shown that the slant-stripe-type MgO: PPLN is capable of realizing the quasi-phase-matching in two perpendicular direction of optical and THz-wave propagation.
ABSTRACT The dried bark of Phellodendron amurense Rupr., known as “Guanhuangbo” in China, has bee... more ABSTRACT The dried bark of Phellodendron amurense Rupr., known as “Guanhuangbo” in China, has been widely used as a traditional Chinese medicine for thousands of years. In this paper, an accurate and reliable method using high performance liquid chromatography coupled with diode array detection and mass spectrometry (HPLC-DAD-MS) was developed for the quality evaluation of wild Guanhuangbo. Six bioactive compounds, namely, chlorogenic acid, phellodendrine, magnoflorine, jatrorrhizine, palmatine and berberine, were determined simultaneously in 37 batches of wild Guanhuangbo samples collected from different locations in China. The chromatographic conditions and extraction procedures were optimized by an orthogonal design during the study and the identities of the compounds were confirmed by LC-MS. Moreover, similarity analysis and hierarchical clustering analysis were successfully applied to demonstrate the variability of these wild Guanhuangbo samples and the data obtained from different analysis showed good consistency. The results indicated that the developed multi-compound determination method in combination with fingerprint analysis is suitable for the quantitative analysis and quality evaluation of the dried bark of wild Phellodendron amurense Rupr.
Longmen Shan tectonic belt located in West Sichuan Plateau, which keeps the good forests outlook ... more Longmen Shan tectonic belt located in West Sichuan Plateau, which keeps the good forests outlook and provides a unique natural environment for the growth of medicinal plants because of its unique geological features. It is a area that is one of the most abundant biological diversity in China and the whole world range, and also is the one of the most concentrated area of the origin of Chinese herbal medicines in Sichuan Province and China. Wenchuan Earthquake induced thousands of landslides, debris flows, which made a huge impact on the medicinal plant habitat by means of severe fragmentation. In this paper, through evaluating and integrating the multi-period remote sensing image data and basic geographic information data of the Longmen Shan region after earthquake by grid technology, the image group that covers the whole Longmen Shan belt and reflects the different features of ground object is acquired. Characters of Wenchuan earthquake disaster distributing is interpreted and analy...
Two new spirobenzofuranoid dibenzocyclooctadiene lignans, kadsulignans O (1) and P (2) were isola... more Two new spirobenzofuranoid dibenzocyclooctadiene lignans, kadsulignans O (1) and P (2) were isolated from the stems of Kadsura oblongifolia, together with five known same type lignans, kadsulignans C (3), E (4), F (5), G (6), and heteroclitin J (7). Their structures were elucidated on the basis of spectral evidences and comparisons with literatures. Compounds 3-7 were isolated from this plant for the first time.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
To provide practical method for microscopic authentication of traditional Chinese medicine Gusuib... more To provide practical method for microscopic authentication of traditional Chinese medicine Gusuibu and its adulterants. By means of light microscope, scanning electron microscopy and tissue section techniques, the morphology, the size of the rhizome scales and their bearing position in the original plants of Gusuibu and its adulterants, i. e. Drynaria roosii, D. delavayi, D. quercifolia and Pseudodrynaria coronans were analyzed. There were significant differences between scales length of D. roosii, D. delavayi and P. coronans, while there was no significant difference between that of D. roosii and D. quercifolia. The scale teeth of D. delavayi were usually curved, bifid and uneven distributed at the scale fringe, which was different from that of the other three species. The base of the scales sinks in epidermis in D. roosii, D. quercifolia, and P. coronans, while it bore at the raised part of epidermis in D. delavayi. CONCLUTION: Morphology, size and bearing position of the rhizome ...
A rapid, sensitive, and accurate reversed-phase high-performance liquid chromatography with photo... more A rapid, sensitive, and accurate reversed-phase high-performance liquid chromatography with photodiode array detection method was developed for both quantitative determination of four compounds (caffeic acid-4-O-β-D-glucopyranoside, 5,7-dihydroxychromone-7-O-rutinoside, neoeriocitrin and naringin) and fingerprint analysis of the rhizomes of Drynaria fortunei (Kunze) J. Sm. The chromatographic separation was accomplished on an MZ-C18 column (4.6 × 250 mm, 5 μm) using gradient elution with acetonitrile and 0.02% aqueous acetic acid, at a flow rate of 1.0 mL min(-1), an operating temperature of 25°C, and a wavelength of 260 nm. The four compounds showed good regression relationship (R (2) > 0.9990) within linear ranges, and their recoveries were in the range of 98.11-102.23%. In the chromatographic fingerprint, thirteen common peaks were found and selected as characteristic peaks to assess the consistency of ten batches of the rhizomes of D. fortunei. The results indicate that the m...
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
To study the procedure of seed quality testing and seed grading scale of Phellodendron amurense. ... more To study the procedure of seed quality testing and seed grading scale of Phellodendron amurense. Seed quality testing methods were developed, which included the test of sampling, seed purity, weight per 1 000 seeds, seed moisture, seed viability and germination rate. The related data from 62 cases of seed specimens of P. amurense were analyzed by cluster analysis. The seed quality test procedure was developed, and the seed quality grading scale was formulated.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
Through the study of allelopathy of the pericarp of Phellodendron amurense, the role of self-rege... more Through the study of allelopathy of the pericarp of Phellodendron amurense, the role of self-regeneration barriers was investigated in order to find ways and means for the protection of wild populations of P. amurense. Solution preparation: soaked pericarp of P. amurense in distilled water at 4 degrees C to get solution A, and reflux extraction of pericarp with distilled water at 100 degrees C to get solution B. Both of the solution A and solution B were used in the experiment of seed germination and seedling growth with the seeds of cabbage and wheat. The results showed that 20 g x L(-1) concentration of solution A and solution B inhibited significantly seed germination of cabbage and wheat, while 100 g x L(-1) concentration of solution A even completely inhibited the seed germination of wheat. 20 g x L(-1) concentration of solution A significantly inhibited the cabbage and wheat seedling growth, completely inhibited the root growth of cabbage, while 100 g x L(-1) concentrations of...
In this paper, we briefly review international Good Agricultural Practice (GAP) regulations relat... more In this paper, we briefly review international Good Agricultural Practice (GAP) regulations related to traditional Chinese medicine herbs (TCM herbs) and the background of the drafting process and the implementation of GAP for TCM herbs in China. We also have summarized progress and achievements since the implementation of GAP for TCM herbs in 2002. Up to 2010, a total of 99 GAP bases were formally adopted by GAP certification. They cover 22 provinces/municipalities, and 49 species of TCM herbs are currently cultivated in these GAP bases. Finally, we discuss the main problems in GAP implementation for Chinese crude drugs and put forward some suggestions to tackle them.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
To investigate and arrange the ethno-pharmacological information of Schisandraceae plants in Chin... more To investigate and arrange the ethno-pharmacological information of Schisandraceae plants in China. The information was obtained by literature search, specimen inspection, field resource investigation and samples collection. Twenty-six Schisandraece plants (inlcuding 4 varieties) have been used as folk medicines in different regions of China, the identical medicinal parts of different species in one genus show the similar usage, action and indications, and different medicinal parts with different administrated ways show different action and indications. The results provide reliable information for resource development and comprehensive utilization of Schisandraece plant resource in China.
A rapid, sensitive, and accurate reversed-phase high-performance liquid chromatography with photo... more A rapid, sensitive, and accurate reversed-phase high-performance liquid chromatography with photodiode array detection method was developed for both quantitative determination of four compounds (caffeic acid-4-O-β-D-glucopyranoside, 5,7-dihydroxychromone-7-O-rutinoside, neoeriocitrin and naringin) and fingerprint analysis of the rhizomes of Drynaria fortunei (Kunze) J. Sm. The chromatographic separation was accomplished on an MZ-C18 column (4.6 × 250 mm, 5 μm) using gradient elution with acetonitrile and 0.02% aqueous acetic acid, at a flow rate of 1.0 mL min(-1), an operating temperature of 25°C, and a wavelength of 260 nm. The four compounds showed good regression relationship (R (2) > 0.9990) within linear ranges, and their recoveries were in the range of 98.11-102.23%. In the chromatographic fingerprint, thirteen common peaks were found and selected as characteristic peaks to assess the consistency of ten batches of the rhizomes of D. fortunei. The results indicate that the m...
Phytomedicine : international journal of phytotherapy and phytopharmacology
In China, the plants of genus Kadsura had been used as the folk medicines for a long time and sho... more In China, the plants of genus Kadsura had been used as the folk medicines for a long time and showed good effect of activating blood and dissolving stasis, promoting qi circulation to relieve pain, dispelling wind and eliminating dampness. The bioactivities of genus Kadsura were attributed to the existence of its characteristic chemical constituents. This review systematically summarized the traditional efficacy and medicinal application of genus Kadsura in China, chemical constituents and bioactivities of the plants of genus Kadsura. And, lignans and triterpenoids were the main bioactive constituents, which exhibited good anti-HIV, anti-tumor, anti-hepatitis, anti-oxidant, anti-platelet aggregation activities and neuroprotective effect etc. Moreover, some structure-activity relationships mining would greatly enrich the opportunity of finding new and promising lead compounds and promote the reasonable development and utilization of the plants of genus Kadsura.
Phytomedicine : international journal of phytotherapy and phytopharmacology, Jan 15, 2014
The stamen of lotus, known as Nelumbinis stamen, has been used as the folk medicine and functiona... more The stamen of lotus, known as Nelumbinis stamen, has been used as the folk medicine and functional food for a long time, which showed good activities of anti-ulcer, anti-thrombosis, analgesic, anti-diarrhea, strengthen uterine contraction. The bioactivities of Nelumbinis stamen were attributed to the existence of flavonoids, its characteristic chemical constituents. A reliable method for comprehensive chemical analysis of flavonoids in Nelumbinis stamen by HPLC-DAD-MS was developed for the first time. The extraction protocol of flavonoids from Nelumbinis stamen was optimized by an orthogonal design. The chromatographic conditions were optimized, which exhibited similar level than that of the UHPLC platform allowing target compound identification in a shorter time with little solvent consumption. Moreover, similarity analysis, hierarchical clustering analysis and principal components analysis were successfully applied to demonstrate the variability of these Nelumbinis stamen samples.
2010 18th International Conference on Geoinformatics, 2010
Longmen Shan tectonic belt located in West Sichuan Plateau, which keeps the good forests outlook ... more Longmen Shan tectonic belt located in West Sichuan Plateau, which keeps the good forests outlook and provides a unique natural environment for the growth of medicinal plants because of its unique geological features. It is a area that is one of the most abundant biological diversity in China and the whole world range, and also is the one of the most concentrated area of the origin of Chinese herbal medicines in Sichuan Province and China. Wenchuan Earthquake induced thousands of landslides, debris flows, which made a huge impact on the medicinal plant habitat by means of severe fragmentation. In this paper, through evaluating and integrating the multi-period remote sensing image data and basic geographic information data of the Longmen Shan region after earthquake by grid technology, the image group that covers the whole Longmen Shan belt and reflects the different features of ground object is acquired. Characters of Wenchuan earthquake disaster distributing is interpreted and analyzed by geographic information system platform for spatial analysis with the image group. Combine with the life habits of medicinal plants in this area and the main ecological factors impacting the spatial distribution pattern of medicinal plants, the impact on distribution pattern of medicinal plants habits during the disaster discussed, as a result, medicinal plants habitat influenced in the shock in various environmental factors is concluded. It can provide the academic evidence for medicinal plant restoration in earthquake zone.
In this paper, we briefly review international Good Agricultural Practice (GAP) regulations relat... more In this paper, we briefly review international Good Agricultural Practice (GAP) regulations related to traditional Chinese medicine herbs (TCM herbs) and the background of the drafting process and the implementation of GAP for TCM herbs in China. We also have summarized progress and achievements since the implementation of GAP for TCM herbs in 2002. Up to 2010, a total of 99 GAP bases were formally adopted by GAP certification. They cover 22 provinces/municipalities, and 49 species of TCM herbs are currently cultivated in these GAP bases. Finally, we discuss the main problems in GAP implementation for Chinese crude drugs and put forward some suggestions to tackle them.
The fruit of S. sphenanthera, known as &a... more The fruit of S. sphenanthera, known as "Nanwuweizi", has been widely used as traditional Chinese medicine for several thousand years. However, the current determination methods are not sufficient to evaluate its quality. An accurate, sensitive and reliable high performance liquid chromatography coupled with photodiode array detection and mass spectrum (HPLC-PAD-MS) was developed for quantitative analysis of twelve lignans (schisandrol A, schisandrol B, gomisin G, schisantherin A, schisantherin D, schisanhenol, (+)-anwulignan, deoxyschisandrin, schisandrin B, schisandrin C, 6-O-benzoylgomisin O, and interiotherin A) in the fruit of S. sphenanthera. The chromatographic conditions and extraction procedures were optimized during the study. The identity of chromatographic peaks in the sample HPLC profiles was confirmed by comparing the retention time, ultraviolet (UV) spectra and MS data with reference compounds. The validated method was successfully used to determine the twelve lignans in the samples collected from different localities in China. The hierarchical clustering analysis (HCA) and principal components analysis (PCA) were successfully applied to the data of twelve lignans from the HPLC profiles in sixteen batches of the fruit of S. sphenanthera to discriminate the samples with different sources. Moreover, the results of the loading plot of the PCA indicated that schisantherin A, (+)-anwulignan, and deoxyshisandrin were found to be the main constituents in the fruit of S. sphenanthera, and which could be chosen as the chemical markers for evaluate the quality of the fruit of S. sphenanthera. The results indicated that the developed method was readily utilized as a quality evaluation method for the fruit of S. sphenanthera.
International Conference on Infrared and Millimeter Waves, 2006
According to the concept of phase matching bandwidth the band width of broadband source by using ... more According to the concept of phase matching bandwidth the band width of broadband source by using retracing behavior of collinear quasi-phase-matching (CQPM) optical parametric generator (OPG) in periodically poled lithium niobate (PPLN) is presented firstly. Comparing the various pump wavelengths we obtain the pumping condition and parameters of PPLN to realize the broadband source near 1550 nm in the CQPM-OPG.
International Conference on Infrared and Millimeter Waves, 2006
Dual-signal-wave optical parametric oscillators (DSW OPOs) around 1.5 mum by using two periodical... more Dual-signal-wave optical parametric oscillators (DSW OPOs) around 1.5 mum by using two periodically poled crystals was demonstrated experimentally. The widely tunable wavelength interval from 2.5 nm to 69.1 nm was obtained. It can be used for generation of terahertz (THz) waves from 0.3-8.9 THz by different frequency generation. With the incident pump power of 3 W at 50 kHz, the
The theory of THz-wave generation (3.29 THz) via difference frequency generation (DFG) of near in... more The theory of THz-wave generation (3.29 THz) via difference frequency generation (DFG) of near infrared radiations (1318.8 nm and 1338.2 nm) by a simultaneous dual-wavelength lasing (SDWL) of an Nd: YAG laser in slant-stripe-type MgO-doped periodically poled lithium niobate (MgO: PPLN) was presented. It is shown that the slant-stripe-type MgO: PPLN is capable of realizing the quasi-phase-matching in two perpendicular direction of optical and THz-wave propagation.
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