diff --git a/.github/workflows/unit_tests.yml b/.github/workflows/unit_tests.yml
index 8181097eedb..a4fd47910c2 100644
--- a/.github/workflows/unit_tests.yml
+++ b/.github/workflows/unit_tests.yml
@@ -21,7 +21,7 @@ jobs:
     runs-on: ${{matrix.os}}
     strategy:
       matrix:
-        python-version: ['3.9', '3.10', '3.11', '3.12', '3.13']
+        python-version: ['3.9', '3.10', '3.11', '3.12', '3.13', '3.14.0-beta.3']
         os: [ubuntu-latest, windows-latest, macos-latest]
       fail-fast: False
     steps:
@@ -36,7 +36,6 @@ jobs:
       - name: Install dependencies
         run: |
           python -W ignore -m pip install --upgrade pip
-          python -W ignore -m pip install -U pytest-cov
           python -W ignore -m pip install . --group tests
 
       - name: Test with pytest
@@ -59,11 +58,10 @@ jobs:
           TO_TEST="test_no_passport.py or test_datetime.py or test_defaults.py or test_jobqueue.py or test_applicationbuilder.py or test_ratelimiter.py or test_updater.py or test_callbackdatacache.py or test_request.py"
           pytest -v --cov -k "${TO_TEST}" --junit-xml=.test_report_no_optionals_junit.xml
           opt_dep_status=$?
-
+          
           # Test the rest
           export TEST_WITH_OPT_DEPS='true'
-          # need to manually install pytz here, because it's no longer in the optional reqs
-          pip install .[all] pytz
+          pip install .[all]
           # `-n auto --dist worksteal` uses pytest-xdist to run tests on multiple CPU
           # workers. Increasing number of workers has little effect on test duration, but it seems
           # to increase flakyness.
diff --git a/changes/unreleased/4825.R7wiTzvN37KAV656s9kfnC.toml b/changes/unreleased/4825.R7wiTzvN37KAV656s9kfnC.toml
new file mode 100644
index 00000000000..5f932e8254d
--- /dev/null
+++ b/changes/unreleased/4825.R7wiTzvN37KAV656s9kfnC.toml
@@ -0,0 +1,5 @@
+other = "Add Python 3.14 Beta To Test Matrix. *Python 3.14 is not officially supported by PTB yet!*"
+[[pull_requests]]
+uid = "4825"
+author_uid = "harshil21"
+closes_threads = []
diff --git a/pyproject.toml b/pyproject.toml
index 66589c25b0e..12c7d5df4d8 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -36,6 +36,7 @@ classifiers = [
     "Programming Language :: Python :: 3.11",
     "Programming Language :: Python :: 3.12",
     "Programming Language :: Python :: 3.13",
+    "Programming Language :: Python :: 3.14",
 ]
 dependencies = [
     "httpx >=0.27,<0.29",
@@ -91,7 +92,7 @@ socks = [
 ]
 webhooks = [
     # tornado is rather stable, but let's not allow the next major release without prior testing
-    "tornado~=6.4",
+    "tornado~=6.5",
 ]
 
 [dependency-groups]
@@ -99,7 +100,7 @@ tests = [
     # required for building the wheels for releases
     "build",
     # For the test suite
-    "pytest==8.3.5",
+    "pytest==8.4.0",
     # needed because pytest doesn't come with native support for coroutines as tests
     "pytest-asyncio==0.21.2",
     # xdist runs tests in parallel
@@ -111,6 +112,10 @@ tests = [
     # For testing with timezones. Might not be needed on all systems, but to ensure that unit tests
     # run correctly on all systems, we include it here.
     "tzdata",
+    # We've deprecated support pytz, but we still need it for testing that it works with the library.
+    "pytz",
+    # Install coverage:
+    "pytest-cov"
 ]
 docs = [
     "chango~=0.4.0; python_version >= '3.12'",
@@ -123,6 +128,13 @@ docs = [
     "sphinx-inline-tabs==2023.4.21",
     # Temporary. See #4387
     "sphinx-build-compatibility @ git+https://github.com/readthedocs/sphinx-build-compatibility.git@58aabc5f207c6c2421f23d3578adc0b14af57047",
+    # For python 3.14 support, we need a version of pydantic-core >= 2.35.0, since it upgrades the
+    # rust toolchain, required for building the project. But there isn't a version of pydantic
+    # which allows that pydantic-core version yet, so we use the latest commit on the
+    # pydantic repository, which has the required version of pydantic-core.
+    # This should ideally be done in `chango`'s dependencies. We can remove this once a new pydantic
+    # version is released.
+    "pydantic @ git+https://github.com/pydantic/pydantic ; python_version >= '3.14'"
 ]
 all = ["pre-commit", { include-group = "tests" }, { include-group = "docs" }]
 
diff --git a/tests/README.rst b/tests/README.rst
index 822c90d35c7..77fbd7b1855 100644
--- a/tests/README.rst
+++ b/tests/README.rst
@@ -42,7 +42,7 @@ such that tests marked with ``@pytest.mark.xdist_group("name")`` are run on the
 
 .. code-block:: bash
 
-    $ pytest -n auto --dist=loadgroup
+    $ pytest -n auto --dist=worksteal
 
 This will result in a significant speedup, but may cause some tests to fail. If you want to run
 the failed tests in isolation, you can use the ``--lf`` flag:
diff --git a/tests/ext/test_filters.py b/tests/ext/test_filters.py
index ae125c98a40..6802db2a206 100644
--- a/tests/ext/test_filters.py
+++ b/tests/ext/test_filters.py
@@ -18,6 +18,7 @@
 # along with this program.  If not, see [http://www.gnu.org/licenses/].
 import datetime as dtm
 import inspect
+import platform
 import re
 
 import pytest
@@ -711,7 +712,9 @@ def test_filters_document_type(self, update):
         assert not filters.Document.WAV.check_update(update)
         assert not filters.Document.AUDIO.check_update(update)
 
-        update.message.document.mime_type = "audio/x-wav"
+        update.message.document.mime_type = (
+            "audio/x-wav" if int(platform.python_version_tuple()[1]) < 14 else "audio/vnd.wave"
+        )
         assert filters.Document.WAV.check_update(update)
         assert filters.Document.AUDIO.check_update(update)
         assert not filters.Document.XML.check_update(update)